EMDB-11361: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cere...

Basic information

• Entry: Database: EMDB / ID: EMD-11361
• Title: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Dis-A (Poly-Ala)

Sample

• Complex: 90S pre-ribbosome state Dis-A
  • Protein or peptide: x 56 types
  • RNA: x 3 types
• Ligand: x 1 types

Function / homology

Function:

18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / Noc4p-Nop14p complex / regulation of ribosomal protein gene transcription by RNA polymerase II / rRNA small subunit pseudouridine methyltransferase Nep1 / t-UTP complex / RNA fragment catabolic process / CURI complex / UTP-C complex / rRNA 2'-O-methylation / Pwp2p-containing subcomplex of 90S preribosome / histone H2AQ104 methyltransferase activity / nuclear microtubule / Mpp10 complex / snoRNA guided rRNA 2'-O-methylation / rRNA (pseudouridine) methyltransferase activity / regulation of rRNA processing / rRNA (adenine-N6,N6-)-dimethyltransferase activity / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA modification / septum digestion after cytokinesis / snRNA binding / positive regulation of RNA binding / SUMOylation of RNA binding proteins / box C/D sno(s)RNA 3'-end processing / tRNA export from nucleus / regulation of transcription by RNA polymerase I / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rDNA heterochromatin / rRNA methyltransferase activity / positive regulation of rRNA processing / box C/D methylation guide snoRNP complex / U4/U6 snRNP / rRNA base methylation / single-stranded telomeric DNA binding / rRNA primary transcript binding / sno(s)RNA-containing ribonucleoprotein complex / U4 snRNA binding / protein localization to nucleolus / O-methyltransferase activity / U4 snRNP / mTORC1-mediated signalling / Protein hydroxylation / rRNA methylation / U3 snoRNA binding / : / poly(A)+ mRNA export from nucleus / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / precatalytic spliceosome / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / snoRNA binding / spliceosomal complex assembly / establishment of cell polarity / positive regulation of transcription by RNA polymerase I / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / nucleolar large rRNA transcription by RNA polymerase I / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / enzyme activator activity / proteasome assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / RNA processing / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / U4/U6 x U5 tri-snRNP complex / RNA endonuclease activity / Transferases; Transferring one-carbon groups; Methyltransferases / nuclear periphery / small-subunit processome / helicase activity / maintenance of translational fidelity / spliceosomal complex / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / mRNA splicing, via spliceosome / ribosomal small subunit assembly / rRNA processing / cytosolic small ribosomal subunit / unfolded protein binding / ribosome biogenesis / cytoplasmic translation / small ribosomal subunit / cytosolic large ribosomal subunit / tRNA binding / RNA helicase activity / rRNA binding / RNA helicase / ribosome / structural constituent of ribosome

Domain/homology:

U3 small nucleolar RNA-associated protein 8 / Utp8 family / U3 small nucleolar RNA-associated protein 20, N-terminal / U3 snoRNA associated / Nucleolar complex protein 4 / U3 small nucleolar RNA-associated protein 20, C-terminal / U3 small nucleolar RNA-associated protein 20, N-terminal / U3 snoRNA associated / U3 small nucleolar RNA-associated protein 20, C-terminal / Nucleolar protein 14 / Nop14-like family / rRNA-processing protein Fcf1, PIN domain / NOL6/Upt22 / Small-subunit processome, Utp11 / Fcf2 pre-rRNA processing, C-terminal / Nrap protein domain 1 / Nrap protein, domain 2 / Nrap protein, domain 3 / Nrap protein, domain 4 / Nrap protein, domain 5 / Nrap protein, domain 6 / Ribosomal RNA-processing protein Rrp9-like / Fcf2/DNTTIP2 / Nrap protein domain 1 / Utp11 protein / Fcf2 pre-rRNA processing / Nrap protein PAP/OAS-like domain / Nrap protein domain 3 / Nrap protein nucleotidyltransferase domain 4 / Nrap protein PAP/OAS1-like domain 5 / Nrap protein domain 6 / rRNA-processing protein Fcf1/Utp23 / Ribosomal RNA-processing protein 7, C-terminal domain / Ribosomal RNA-processing protein 7 / Rrp7, RRM-like N-terminal domain / Fcf1 / Ribosomal RNA-processing protein 7 (RRP7) C-terminal domain / Rrp7 RRM-like N-terminal domain / Small-subunit processome, Utp14 / U3 small nucleolar RNA-associated protein 15, C-terminal / Utp14 protein / UTP15 C terminal / Sas10 C-terminal domain / Sas10 C-terminal domain / Nucleolar protein 58/56, N-terminal / U3 small nucleolar RNA-associated protein 18 / NOP5NT (NUC127) domain / BP28, C-terminal domain / RNA 3'-terminal phosphate cyclase-like, conserved site / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosome biogenesis protein Bms1, N-terminal / U3 small nucleolar RNA-associated protein 10 / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 10 / RNA 3'-terminal phosphate cyclase signature. / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 13, C-terminal / Periodic tryptophan protein 2 / Utp13 specific WD40 associated domain / Small-subunit processome, Utp12 / Dip2/Utp12 Family / Sas10/Utp3/C1D / Small-subunit processome, Utp21 / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / RNA 3'-terminal phosphate cyclase type 2 / Sas10/Utp3/C1D family / Mpp10 protein / Utp21 specific WD40 associated putative domain / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / EMG1/NEP1 methyltransferase / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase, insert domain / RNA 3'-terminal phosphate cyclase domain / RNA 3'-terminal phosphate cyclase, insert domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase (RTC), insert domain / Ribosomal RNA adenine dimethylase / Nucleolar protein Nop56/Nop58 / CCAAT-binding factor / CBF/Mak21 family / Krr1, KH1 domain / Krr1 KH1 domain / Bystin / Bystin / rRNA 2'-O-methyltransferase fibrillarin-like / Fibrillarin, conserved site / Fibrillarin / Fibrillarin signature. / Fibrillarin / Ribosomal RNA adenine methylase transferase, conserved site / Ribosomal RNA adenine methylase transferase, N-terminal / Ribosomal RNA adenine dimethylases signature. / Ribosomal RNA adenine dimethylases / Ribosomal RNA adenine methyltransferase KsgA/Erm / Ribosomal RNA adenine dimethylase / rRNA adenine N(6)-methyltransferase family profile. / U3 snoRNP protein/Ribosome production factor 1 / Large family of predicted nucleotide-binding domains / Ribosome biogenesis protein BMS1/TSR1, C-terminal

Component:

Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / rRNA 2'-O-methyltransferase fibrillarin / Ribosomal RNA-processing protein 7 / Periodic tryptophan protein 2 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7A / U3 small nucleolar ribonucleoprotein protein IMP3 / Small ribosomal subunit protein eS1A / U3 small nucleolar RNA-associated protein 11 / U3 small nucleolar RNA-associated protein 20 / Small ribosomal subunit protein eS27A / Essential nuclear protein 1 / U3 small nucleolar RNA-associated protein 9 / 13 kDa ribonucleoprotein-associated protein / U3 small nucleolar RNA-associated protein 18 / Dimethyladenosine transferase / U3 small nucleolar RNA-associated protein 10 / U3 small nucleolar RNA-associated protein MPP10 / U3 small nucleolar RNA-associated protein 22 / U3 small nucleolar RNA-associated protein 8 / U3 small nucleolar ribonucleoprotein protein IMP4 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / Probable ATP-dependent RNA helicase DHR1 / U3 small nucleolar RNA-associated protein 15 / U3 small nucleolar RNA-associated protein 14 / rRNA-processing protein FCF1 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / Ribosomal RNA-processing protein 9 / Nucleolar complex protein 4 / U3 small nucleolar RNA-associated protein 4 / RNA 3'-terminal phosphate cyclase-like protein / U3 small nucleolar RNA-associated protein 16 / Ribosome biogenesis protein BMS1 / rRNA-processing protein FCF2 / Something about silencing protein 10 / U3 small nucleolar RNA-associated protein 12 / Nucleolar protein 56 / Nucleolar protein 58 / Small ribosomal subunit protein eS28A / Nucleolar complex protein 14 / Pre-rRNA-processing protein PNO1

• Biological species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) / Baker's yeast (brewer's yeast) / Saccharomyces cerevisiae S288C (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 7.1 Å
• Authors: Cheng J / Lau B / Venuta GL / Berninghausen O / Hurt E / Beckmann R
• Citation: Journal: Science / Year: 2020; Title: 90 pre-ribosome transformation into the primordial 40 subunit.; Authors: Jingdong Cheng / Benjamin Lau / Giuseppe La Venuta / Michael Ameismeier / Otto Berninghausen / Ed Hurt / Roland Beckmann / ; Abstract: Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this process by biochemical and cryo-electron microscopy analysis of intermediates along this pathway in yeast. First, the remodeling RNA helicase Dhr1 engages the 90 pre-ribosome, followed by Utp24 endonuclease-driven RNA cleavage at site A, thereby separating the 5'-external transcribed spacer (ETS) from 18 ribosomal RNA. Next, the 5'-ETS and 90 assembly factors become dislodged, but this occurs sequentially, not en bloc. Eventually, the primordial pre-40 emerges, still retaining some 90 factors including Dhr1, now ready to unwind the final small nucleolar U3-18 RNA hybrid. Our data shed light on the elusive 90 to pre-40 transition and clarify the principles of assembly and remodeling of large ribonucleoproteins.

History

Deposition	Jul 9, 2020	-
Header (metadata) release	Sep 23, 2020	-
Map release	Sep 23, 2020	-
Update	Sep 30, 2020	-
Current status	Sep 30, 2020	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_11361.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.059 Å

Density

Contour Level	By AUTHOR: 0.005 / Movie #1: 0.005
Minimum - Maximum	-0.012674955 - 0.036449794
Average (Standard dev.)	0.00027297647 (±0.0016071454)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 508.32 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.059	1.059	1.059
M x/y/z	480	480	480
origin x/y/z	0.000	0.000	0.000
length x/y/z	508.320	508.320	508.320
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	100	115	122
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	480	480	480
D min/max/mean	-0.013	0.036	0.000

Supplemental data

Sample components

Entire : 90S pre-ribbosome state Dis-A

• Entire: Name: 90S pre-ribbosome state Dis-A

Components

• Complex: 90S pre-ribbosome state Dis-A
  • Protein or peptide: Periodic tryptophan protein 2
  • Protein or peptide: Nucleolar complex protein 14
  • Protein or peptide: Something about silencing protein 10
  • Protein or peptide: U3 small nucleolar RNA-associated protein 4
  • Protein or peptide: U3 small nucleolar RNA-associated protein 9
  • Protein or peptide: U3 small nucleolar RNA-associated protein 8
  • Protein or peptide: U3 small nucleolar RNA-associated protein 5
  • Protein or peptide: U3 small nucleolar RNA-associated protein 10
  • Protein or peptide: U3 small nucleolar RNA-associated protein 11
  • Protein or peptide: U3 small nucleolar RNA-associated protein 12
  • Protein or peptide: U3 small nucleolar RNA-associated protein 13
  • Protein or peptide: U3 small nucleolar RNA-associated protein 14
  • Protein or peptide: U3 small nucleolar RNA-associated protein 15
  • Protein or peptide: Bud site selection protein 21
  • Protein or peptide: NET1-associated nuclear protein 1
  • Protein or peptide: U3 small nucleolar RNA-associated protein 18
  • Protein or peptide: Nucleolar complex protein 4
  • Protein or peptide: U3 small nucleolar RNA-associated protein 20
  • Protein or peptide: U3 small nucleolar RNA-associated protein 21
  • Protein or peptide: U3 small nucleolar RNA-associated protein 22
  • Protein or peptide: rRNA-processing protein FCF1
  • Protein or peptide: rRNA 2'-O-methyltransferase fibrillarin
  • Protein or peptide: Nucleolar protein 56Nucleolus
  • Protein or peptide: Nucleolar protein 58Nucleolus
  • Protein or peptide: 13 kDa ribonucleoprotein-associated protein
  • Protein or peptide: Ribosomal RNA-processing protein 9
  • Protein or peptide: U3 small nucleolar ribonucleoprotein protein IMP3
  • Protein or peptide: U3 small nucleolar ribonucleoprotein protein IMP4
  • Protein or peptide: U3 small nucleolar RNA-associated protein MPP10
  • Protein or peptide: Ribosome biogenesis protein BMS1
  • Protein or peptide: RNA 3'-terminal phosphate cyclase-like protein
  • Protein or peptide: Ribosomal RNA-processing protein 7
  • Protein or peptide: Probable ATP-dependent RNA helicase DHR1
  • Protein or peptide: Ribosomal RNA small subunit methyltransferase NEP1
  • Protein or peptide: Essential nuclear protein 1
  • Protein or peptide: Dimethyladenosine transferase
  • Protein or peptide: rRNA-processing protein FCF2
  • Protein or peptide: 40S ribosomal protein S27-ARibosome
  • Protein or peptide: Pre-rRNA-processing protein PNO1
  • Protein or peptide: 40S ribosomal protein S1-ARibosome
  • Protein or peptide: 40S ribosomal protein S4-ARibosome
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S6-ARibosome
  • Protein or peptide: 40S ribosomal protein S7-ARibosome
  • Protein or peptide: 40S ribosomal protein S8-ARibosome
  • Protein or peptide: 40S ribosomal protein S9-ARibosome
  • Protein or peptide: 40S ribosomal protein S11-ARibosome
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S14-ARibosome
  • Protein or peptide: 40S ribosomal protein S16-ARibosome
  • Protein or peptide: 40S ribosomal protein S18-ARibosome
  • Protein or peptide: 40S ribosomal protein S19-ARibosome
  • Protein or peptide: 40S ribosomal protein S22-ARibosome
  • Protein or peptide: 40S ribosomal protein S23-ARibosome
  • Protein or peptide: 40S ribosomal protein S24-ARibosome
  • Protein or peptide: 40S ribosomal protein S28-ARibosome
  • RNA: 5ETS RNA
  • RNA: 18S rRNA18S ribosomal RNA
  • RNA: U3 snoRNA
• Ligand: ZINC ION

Supramolecule #1: 90S pre-ribbosome state Dis-A

• Supramolecule: Name: 90S pre-ribbosome state Dis-A / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#59
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

Macromolecule #1: Periodic tryptophan protein 2

• Macromolecule: Name: Periodic tryptophan protein 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 104.097039 KDa

Sequence

String:

MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF EYEHRKNIAA IDLNKQGTLL ISIDEDGRAI LVNFKARNV LHHFNFKEKC SAVKFSPDGR LFALASGRFL QIWKTPDVNK DRQFAPFVRH RVHAGHFQDI TSLTWSQDSR F ILTTSKDL SAKIWSVDSE EKNLAATTFN GHRDYVMGAF FSHDQEKIYT VSKDGAVFVW EFTKRPSDDD DNESEDDDKQ EE VDISKYS WRITKKHFFY ANQAKVKCVT FHPATRLLAV GFTSGEFRLY DLPDFTLIQQ LSMGQNPVNT VSVNQTGEWL AFG SSKLGQ LLVYEWQSES YILKQQGHFD STNSLAYSPD GSRVVTASED GKIKVWDITS GFCLATFEEH TSSVTAVQFA KRGQ VMFSS SLDGTVRAWD LIRYRNFRTF TGTERIQFNC LAVDPSGEVV CAGSLDNFDI HVWSVQTGQL LDALSGHEGP VSCLS FSQE NSVLASASWD KTIRIWSIFG RSQQVEPIEV YSDVLALSMR PDGKEVAVST LKGQISIFNI EDAKQVGNID CRKDII SGR FNQDRFTAKN SERSKFFTTI HYSFDGMAIV AGGNNNSICL YDVPNEVLLK RFIVSRNMAL NGTLEFLNSK KMTEAGS LD LIDDAGENSD LEDRIDNSLP GSQRGGDLST RKMRPEVRVT SVQFSPTANA FAAASTEGLL IYSTNDTILF DPFDLDVD V TPHSTVEALR EKQFLNALVM AFRLNEEYLI NKVYEAIPIK EIPLVASNIP AIYLPRILKF IGDFAIESQH IEFNLIWIK ALLSASGGYI NEHKYLFSTA MRSIQRFIVR VAKEVVNTTT DNKYTYRFLV STDGSMEDGA ADDDEVLLKD DADEDNEENE ENDVVMESD DEEGWIGFNG KDNKLPLSNE NDSSDEEENE KELP

Macromolecule #2: Nucleolar complex protein 14

• Macromolecule: Name: Nucleolar complex protein 14 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 94.463195 KDa

Sequence

String:

MAGSQLKNLK AALKARGLTG QTNVKSKNKK NSKRQAKEYD REEKKKAIAE IREEFNPFEI KAARNKRRDG LPSKTADRIA VGKPGISKQ IGEEQRKRAF EARKMMKNKR GGVIDKRFGE RDKLLTEEEK MLERFTRERQ SQSKRNANLF NLEDDEDDGD M FGDGLTHL GQSLSLEDEL ANDEEDFLAS KRFNEDDAEL QQPQRKKTKA EVMKEVIAKS KFYKQERQKA QGIMEDQIDN LD DNFEDVM SELMMTQPKK NPMEPKTDLD KEYDIKVKEL QLDKRAAPSD RTKTEEEKNA EAEEKKRELE QQRLDRMNGM IEL EEGEER GVEDLDDGFW ENEEDYEDDN DGIADSDDDI KFEDQGRDEG FSQILKKKNI SISCPRTHDA LLDQVKKLDL DDHP KIVKN IIKAYQPKLA EGNKEKLGKF TAVLLRHIIF LSNQNYLKNV QSFKRTQNAL ISILKSLSEK YNRELSEECR DYINE MQAR YKKNHFDALS NGDLVFFSII GILFSTSDQY HLVITPALIL MSQFLEQIKF NSLKRIAFGA VLVRIVSQYQ RISKRY IPE VVYFFQKILL TFIVEKENQE KPLDFENIRL DSYELGLPLD VDFTKKRSTI IPLHTLSTMD TEAHPVDQCV SVLLNVM ES LDATISTVWK SLPAFNEIIL PIQQLLSAYT SKYSDFEKPR NILNKVEKLT KFTEHIPLAL QNHKPVSIPT HAPKYEEN F NPDKKSYDPD RTRSEINKMK AQLKKERKFT MKEIRKDAKF EARQRIEEKN KESSDYHAKM AHIVNTINTE EGAEKNKYE RERKLRGGKK

Macromolecule #3: Something about silencing protein 10

• Macromolecule: Name: Something about silencing protein 10 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 70.364398 KDa

Sequence

String:

MVRKGSNRTK TSEVGDEINP YGLNEVDDFA SKREKVLLGQ STFGDSNKDD DHSLLEDEDE EEVLAMDEDD ESIDEREDEE EEEEEELDG AAAYKKIFGR NLETDQLPEE DEENGMLDNE NAWGSTKGEY YGADDLDDDE AAKEIEKEAL RQQKKHLEEL N MNDYLDEE EEEEWVKSAK EFDMGEFKNS TKQADTKTSI TDILNMDDEA RDNYLRTMFP EFAPLSKEFT ELAPKFDELK KS EENEFNK LKLIALGSYL GTISCYYSIL LHELHNNEDF TSMKGHPVME KILTTKEIWR QASELPSSFD VNEGDGSESE ETA NIEAFN EKKLNELQNS EDSDAEDGGK QKQEIDEEER ESDEEEEEED VDIDDFEEYV AQSRLHSKPK TSSMPEADDF IESE IADVD AQDKKARRRT LRFYTSKIDQ QENKKTDRFK GDDDIPYKER LFERQQRLLD EARKRGMHDN NGADLDDKDY GSEDE AVSR SINTQGENDY YQQVQRGKQD KKISRKEAHK NAVIAAREGK LAELAENVSG DGKRAINYQI LKNKGLTPKR NKDNRN SRV KKRKKYQKAQ KKLKSVRAVY SGGQSGVYEG EKTGIKKGLT RSVKFKN

Macromolecule #4: U3 small nucleolar RNA-associated protein 4

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 87.909242 KDa

Sequence

String:

MSSSLLSVLK EKSRSLKIRN KPVKMTSQER MIVHRCRFVD FTPATITSLA FSHKSNINKL TPSDLRLAIG RSNGNIEIWN PRNNWFQEM VIEGGKDRSI EGLCWSNVNG ESLRLFSIGG STVVTEWDLA TGLPLRNYDC NSGVIWSISI NDSQDKLSVG C DNGTVVLI DISGGPGVLE HDTILMRQEA RVLTLAWKKD DFVIGGCSDG RIRIWSAQKN DENMGRLLHT MKVDKAKKES TL VWSVIYL PRTDQIASGD STGSIKFWDF QFATLNQSFK AHDADVLCLT TDTDNNYVFS AGVDRKIFQF SQNTNKSQKN NRW VNSSNR LLHGNDIRAI CAYQSKGADF LVSGGVEKTL VINSLTSFSN GNYRKMPTVE PYSKNVLVNK EQRLVVSWSE STVK IWTMG TDSSTEQNYK LVCKLTLKDD QNISTCSLSP DGQVLVVGRP STTKVFHLQP VGNKLKVTKL DNDLLLRTST KLVKF IDNS KIVICSCEDD VFIVDLESEE DEKPQEVELL EVTSTKSSIK VPYINRINHL EVDQNIAVIS RGCGVVDILD LKARIS KPL ARLNNFITAV HINTSRKSVV VITADNKIYE FNMNLNSEAE NEDSESVLTQ WSKNNTDNLP KEWKTLKENC VGIFSDI EN SSRLWFWGAT WISRIDFDVD FPINKRRKQK KRTHEGLTIT DESNFMNDEE DDEDDDIDME ISENLNVLLN QGNKIKST D VQRNEESSGH FFFTDKYKPL LFVDLISSNE LAIIERNPLT FHSKQKAFIQ PKLVF

Macromolecule #5: U3 small nucleolar RNA-associated protein 9

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 9 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 65.347254 KDa

Sequence

String:

MGSSLDLVAS FSHDSTRFAF QASVAQKNNV DIYPLNETKD YVVNSSLVSH IDYETNDMKV SDVIFFGWCS DLIDTQSSNI KRKLDEDEG TGESSEQRCE NFFVNGFPDG RIVVYSSNGK DIVNIIKNKK EILGADTDES DIWILDSDKV VKKLQYNNSK P LKTFTLVD GKDDEIVHFQ ILHQNGTLLV CIITKQMVYI VDPSKRRPST KYSFEISDAV ACEFSSDGKY LLIANNEELI AY DLKEDSK LIQSWPVQVK TLKTLDDLIM ALTTDGKINN YKIGEADKVC SIVVNEDLEI IDFTPINSKQ QVLISWLNVN EPN FESISL KEIETQGYIT INKNEKNNAD EADQKKLEEK EEEAQPEVQH EKKETETKIN KKVSKSDQVE IANILSSHLE ANST EILDD LMSGSWTEPE IKKFILTKIN TVDHLSKIFL TISKSITQNP WNEENLLPLW LKWLLTLKSG ELNSIKDKHT KKNCK HLKS ALRSSEEILP VLLGIQGRLE MLRRQAKLRE DLAQLSMQEG EDDEIEVIEH SNVISNPLQD QASPVEKLEP DSIVYA NGE SDEFVDASEY KD

Macromolecule #6: U3 small nucleolar RNA-associated protein 8

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 8 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 80.269648 KDa

Sequence

String:

MPSLSQPFRL ATLPKIASLS NFSLQADYVQ VADGTFNEST NNITLGISGS SISQYIINPT PKLTFDYPIP STNIITACNA EKGQANIDG NIEASTDDEA NNEKTINTQK KRNVEIWAFG LMVNKGNYTL NVITKALEDT TDTSNDHLSE SDIDNKAYTG S DEFLSQYK IKAKAKVMSI KIDTKNSLVI AILQNGLIEI FDFKLTLLHS FDISYDNLKY AKWFTENGTE YVFVLCPLQD DK VCYKLLE LTDCGSGESS PIKELSSTII EGFSFENSKL CYQFGKLYKL NQGKIYIYSL PHCQLQQVIE FPMVDKLSPG DDL ISFQPV SVNRVLLTVN NVIYLLDLLH CSTLSQRELT HVKTFQLLKS AVINSEKSHN SKTIAIGIST KNGPNPTSSL EIIN IDVGT NTLKDSLGKS FQVGNNDSSV ILKPLFDDKD INDKRVKCND VSGDSSVPVL HCNEVIEKLS ALQDNDITSF DDIFF KELK IKEEHYTEKD RYISDPGFLN KVLDLIFGKF SGNDYPKTLT FLLTHPLFPL SRTRNLLSLL RDQPRLFKQA IVTCPN LPL NELLEELFSI RNRELLLDIS FRILQDFTRD SIKQEMKKLS KLDVQNFIEF ITSGGEDSSP ECFNPSQSTQ LFQLLSL VL DSIGLFSLEG ALLENLTLYI DKQVEIAERN TELWNLIDTK GFQHGFASST FDNGTSQKRA LPTYTMEYLD I

Macromolecule #7: U3 small nucleolar RNA-associated protein 5

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 5 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 72.079445 KDa

Sequence

String:

MDSPVLQSAY DPSGQYLCYV TVALDKQRVG VQPTQRATSS GVDTVWNENF LYLEDSKLKV TCLKWVNLAS SDTVAIILGM NNGEIWLYS VLANEVTYKF TTGNSYEIKD IDLMGNQLWC IDSSDAFYQF DLLQFKLLQH FRINNCVQLN KLTIVPAGDS V AQLLVASH SISLIDIEEK KVVMTFPGHV SPVSTLQVIT NEFFISGAEG DRFLNVYDIH SGMTKCVLVA ESDIKELSHS GQ ADSIAVT TEDGSLEIFV DPLVSSSTKK RGNKSKKSSK KIQIVSKDGR KVPIYNAFIN KDLLNVSWLQ NATMPYFKNL QWR EIPNEY TVEISLNWNN KNKSADRDLH GKDLASATNY VEGNARVTSG DNFKHVDDAI KSWERELTSL EQEQAKPPQA NELL TETFG DKLESSTVAR ISGKKTNLKG SNLKTATTTG TVTVILSQAL QSNDHSLLET VLNNRDERVI RDTIFRLKPA LAVIL LERL AERIARQTHR QGPLNVWVKW CLIIHGGYLV SIPNLMSTLS SLHSTLKRRS DLLPRLLALD ARLDCTINKF KTLNYE AGD IHSSEPVVEE DEDDVEYNEE LDDAGLIEDG EESYGSEEEE EGDSDNEEEQ KHTSSKQDGR LETEQSDGEE EAGYSDV EM E

Macromolecule #8: U3 small nucleolar RNA-associated protein 10

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 10 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 200.298984 KDa

Sequence

String:

MSSLSDQLAQ VASNNATVAL DRKRRQKLHS ASLIYNSKTA ATQDYDFIFE NASKALEELS QIEPKFAIFS RTLFSESSIS LDRNVQTKE EIKDLDNAIN AYLLLASSKW YLAPTLHATE WLVRRFQIHV KNTEMLLLST LNYYQTPVFK RILSIIKLPP L FNCLSNFV RSEKPPTALT MIKLFNDMDF LKLYTSYLDQ CIKHNATYTN QLLFTTCCFI NVVAFNSNND EKLNQLVPIL LE ISAKLLA SKSKDCQIAA HTILVVFATA LPLKKTIILA AMETILSNLD AKEAKHSALL TICKLFQTLK GQGNVDQLPS KIF KLFDSK FDTVSILTFL DKEDKPVCDK FITSYTRSIA RYDRSKLNII LSLLKKIRLE RYEVRLIITD LIYLSEILED KSQL VELFE YFISINEDLV LKCLKSLGLT GELFEIRLTT SLFTNADVNT DIVKQLSDPV ETTKKDTASF QTFLDKHSEL INTTN VSML TETGERYKKV LSLFTEAIGK GYKASSFLTS FFTTLESRIT FLLRVTISPA APTALKLISL NNIAKYINSI EKEVNI FTL VPCLICALRD ASIKVRTGVK KILSLIAKRP STKHYFLSDK LYGENVTIPM LNPKDSEAWL SGFLNEYVTE NYDISRI LT PKRNEKVFLM FWANQALLIP SPYAKTVLLD NLNKSPTYAS SYSSLFEEFI SHYLENRSSW EKSCIANKTN FEHFERSL V NLVSPKEKQS FMIDFVLSAL NSDYEQLANI AAERLISIFA SLNNAQKLKI VQNIVDSSSN VESSYDTVGV LQSLPLDSD IFVSILNQNS ISNEMDQTDF SKRRRRRSST SKNAFLKEEV SQLAELHLRK LTIILEALDK VRNVGSEKLL FTLLSLLSDL ETLDQDGGL PVLYAQETLI SCTLNTITYL KEHGCTELTN VRADILVSAI RNSASPQVQN KLLLVIGSLA TLSSEVILHS V MPIFTFMG AHSIRQDDEF TTKVVERTIL TVVPALIKNS KGNEKEEMEF LLLSFTTALQ HVPRHRRVKL FSTLIKTLDP VK ALGSFLF LIAQQYSSAL VNFKIGEARI LIEFIKALLV DLHVNEELSG LNDLLDIIKL LTSSKSSSEK KKSLESRVLF SNG VLNFSE SEFLTFMNNT FEFINKITEE TDQDYYDVRR NLRLKVYSVL LDETSDKKLI RNIREEFGTL LEGVLFFINS VELT FSCIT SQENEEASDS ETSLSDHTTE IKEILFKVLG NVLQILPVDE FVNAVLPLLS TSTNEDIRYH LTLVIGSKFE LEGSE AIPI VNNVMKVLLD RMPLESKSVV ISQVILNTMT ALVSKYGKKL EGSILTQALT LATEKVSSDM TEVKISSLAL ITNCVQ VLG VKSIAFYPKI VPPSIKLFDA SLADSSNPLK EQLQVAILLL FAGLIKRIPS FLMSNILDVL HVIYFSREVD SSIRLSV IS LIIENIDLKE VLKVLFRIWS TEIATSNDTV AVSLFLSTLE STVENIDKKS ATSQSPIFFK LLLSLFEFRS ISSFDNNT I SRIEASVHEI SNSYVLKMND KVFRPLFVIL VRWAFDGEGV TNAGITETER LLAFFKFFNK LQENLRGIIT SYFTYLLEP VDMLLKRFIS KDMENVNLRR LVINSLTSSL KFDRDEYWKS TSRFELISVS LVNQLSNIEN SIGKYLVKAI GALASNNSGV DEHNQILNK LIVEHMKASC SSNEKLWAIR AMKLIYSKIG ESWLVLLPQL VPVIAELLED DDEEIEREVR TGLVKVVENV L GEPFDRYL D

Macromolecule #9: U3 small nucleolar RNA-associated protein 11

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 11 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 29.806348 KDa

Sequence

String:

MAKLVHDVQK KQHRERSQLT SRSRYGFLEK HKDYVKRAQD FHRKQSTLKV LREKAKERNP DEYYHAMHSR KTDAKGLLIS SRHGDEEDE SLSMDQVKLL KTQDSNYVRT LRQIELKKLE KGAKQLMFKS SGNHTIFVDS REKMNEFTPE KFFNTTSEMV N RSENRLTK DQLAQDISNN RNASSIMPKE SLDKKKLKKF KQVKQHLQRE TQLKQVQQRM DAQRELLKKG SKKKIVDSSG KI SFKWKKQ RKR

Macromolecule #10: U3 small nucleolar RNA-associated protein 12

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 12 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 106.481133 KDa

Sequence

String:

MVKSYQRFEQ AAAFGVIASN ANCVWIPASS GNSNGSGPGQ LITSALEDVN IWDIKTGDLV SKLSDGLPPG ASDARGAKPA ECTYLEAHK DTDLLAVGYA DGVIKVWDLM SKTVLLNFNG HKAAITLLQF DGTGTRLISG SKDSNIIVWD LVGEVGLYKL R SHKDSITG FWCQGEDWLI STSKDGMIKL WDLKTHQCIE THIAHTGECW GLAVKDDLLI TTGTDSQVKI WKLDIENDKM GG KLTEMGI FEKQSKQRGL KIEFITNSSD KTSFFYIQNA DKTIETFRIR KEEEIARGLK KREKRLKEKG LTEEEIAKSI KES YSSFIL HPFQTIRSLY KIKSASWTTV SSSKLELVLT TSSNTIEYYS IPYEKRDPTS PAPLKTHTIE LQGQRTDVRS IDIS DDNKL LATASNGSLK IWNIKTHKCI RTFECGYALT CKFLPGGLLV ILGTRNGELQ LFDLASSSLL DTIEDAHDAA IWSLD LTSD GKRLVTGSAD KTVKFWDFKV ENSLVPGTKN KFLPVLKLHH DTTLELTDDI LCVRVSPDDR YLAISLLDNT VKVFFL DSM KFYLSLYGHK LPVLSIDISF DSKMIITSSA DKNIKIWGLD FGDCHKSLFA HQDSIMNVKF LPQSHNFFSC SKDAVVK YW DGEKFECIQK LYAHQSEVWA LAVATDGGFV VSSSHDHSIR IWEETEDQVF LEEEKEKELE EQYEDTLLTS LEEGNGDD A FKADASGEGV EDEASGVHKQ TLESLKAGER LMEALDLGIA EIEGLEAYNR DMKLWQRKKL GEAPIKPQGN AVLIAVNKT PEQYIMDTLL RIRMSQLEDA LMVMPFSYVL KFLKFIDTVM QNKTLLHSHL PLICKNLFFI IKFNHKELVS QKNEELKLQI NRVKTELRS ALKSTEDDLG FNVQGLKFVK QQWNLRHNYE FVDEYDQQEK ESNSARKRVF GTVI

Macromolecule #11: U3 small nucleolar RNA-associated protein 13

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 91.132562 KDa

Sequence

String:

MDLKTSYKGI SLNPIYAGSS AVATVSENGK ILATPVLDEI NIIDLTPGSR KILHKISNED EQEITALKLT PDGQYLTYVS QAQLLKIFH LKTGKVVRSM KISSPSYILD ADSTSTLLAV GGTDGSIIVV DIENGYITHS FKGHGGTISS LKFYGQLNSK I WLLASGDT NGMVKVWDLV KRKCLHTLQE HTSAVRGLDI IEVPDNDEPS LNLLSGGRDD IINLWDFNMK KKCKLLKTLP VN QQVESCG FLKDGDGKRI IYTAGGDAIF QLIDSESGSV LKRTNKPIEE LFIIGVLPIL SNSQMFLVLS DQTLQLINVE EDL KNDEDT IQVTSSIAGN HGIIADMRYV GPELNKLALA TNSPSLRIIP VPDLSGPEAS LPLDVEIYEG HEDLLNSLDA TEDG LWIAT ASKDNTAIVW RYNENSCKFD IYAKYIGHSA AVTAVGLPNI VSKGYPEFLL TASNDLTIKK WIIPKPTASM DVQII KVSE YTRHAHEKDI NALSVSPNDS IFATASYDKT CKIWNLENGE LEATLANHKR GLWDVSFCQY DKLLATSSGD KTVKIW SLD TFSVMKTLEG HTNAVQRCSF INKQKQLISC GADGLIKIWD CSSGECLKTL DGHNNRLWAL STMNDGDMIV SADADGV FQ FWKDCTEQEI EEEQEKAKLQ VEQEQSLQNY MSKGDWTNAF LLAMTLDHPM RLFNVLKRAL GESRSRQDTE EGKIEVIF N EELDQAISIL NDEQLILLMK RCRDWNTNAK THTIAQRTIR CILMHHNIAK LSEIPGMVKI VDAIIPYTQR HFTRVDNLV EQSYILDYAL VEMDKLF

Macromolecule #12: U3 small nucleolar RNA-associated protein 14

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 1.518649 KDa

Sequence

String:

TTLPGWGEWA GAGSK

Macromolecule #13: U3 small nucleolar RNA-associated protein 15

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 57.765289 KDa

Sequence

String:

MSTARPRIIT SKAPLLPQQT TPEQRYWRQY TSAQLVKEHN SVTHISFNPQ HPHDFAVTSS TRVQIFSSRT RQVIKTFSRF KDVVYSASF RSDGKLLCAG DATGLVSVYD SYNPRTILLS INASTHPTHV TKFHTQDNKI LATASDDRVT RLWDISNAYE P QLELTGAT DYVRTLSFIP AAPHLVATGS YDGLIRLYDT RSSGSTPIYS LNHDQPVENV IAVSPTQIVS CGGNNFKVWD LT SNKKLYE RGNFNKAVTC LDYVENFDSP MQSALIASSL DGHVKVFDPL DNFQVKFGWK FSGPVLSCAV SPSTAQGNRH LVA GLSSGL LAIRTKKKEK RSSDKENAPA SFNKNAKSNN FQRMMRGSEY QGDQEHIIHN DKVRSQRRMR AFERNINQFK WSEA LDNAF VPGMAKELTL TVLQELRKRG KVRVALYGRD ESTLEPLLNW CLKGIEDVRS ASIVADWVAV VLELYGNTLE SSPVL QELM IDLKTKVRHE IHKSKEAQRI EGMLQLLTS

Macromolecule #14: Bud site selection protein 21

• Macromolecule: Name: Bud site selection protein 21 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.431016 KDa

Sequence

String:

MSNGHVKFDA DESQASASAV TDRQDDVLVI SKKDKEVHSS SDEESDDDDA PQEEGLHSGK SEVESQITQR EEAIRLEQSQ LRSKRRKQN ELYAKQKKSV NETEVTDEVI AELPEELLKN IDQKDEGSTQ YSSSRHVTFD KLDESDENEE ALAKAIKTKK R KTLKNLRK DSVKRGKFRV QLLSTTQDSK TLPPKKESSI IRSKDRWLNR KALNKG

Macromolecule #15: NET1-associated nuclear protein 1

• Macromolecule: Name: NET1-associated nuclear protein 1 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 101.341734 KDa

Sequence

String:

MTQSLGIEQY KLSVVSGGKP ALNNLSSVTG NKNIARLSQD QRNYIIPFNN QIKVYSVETR QCVKTLKFAN NSLLSGIFLQ EEENNESIV KILLGDITVP QQEDAHLITV FTNNGHVIVL NYKGKLVESP KHFKISLADE KLANVFHSEG NYRILTTFKD P SQKAHNSL QSYRLYALTF DDAKKQFEVA HQAEWHNVIL SNISSNGKLL AHMCKDVSTK DHEHKSISVV SLFDDSVNLS FP LGSILSS QTQSLSYNTR YVSSMAIDNM GQQLAVGFAS GVISIVSLAD LQIRLLKWHI DSVLSLSFSH DGSYLLSGGW EKV MSLWQL ETNSQQFLPR LNGIIIDCQV LGPQGNYYSL ILQMTENNSN SDYQFLLLNA SDLTSKLSIN GPLPVFNSTI KHIQ QPISA MNTKNSNSIT SLNHSKKKQS RKLIKSRRQD FTTNVEINPI NKNLYFPHIS AVQIFDFYKN EQVNYQYLTS GVNNS MGKV RFELNLQDPI ITDLKFTKDG QWMITYEIEY PPNDLLSSKD LTHILKFWTK NDNETNWNLK TKVINPHGIS VPITKI LPS PRSVNNSQGC LTADNNGGLK FWSFDSHESN WCLKKISLPN FNHFSNSVSL AWSQDGSLIF HGFDDKLQIL DFDTFKK FE SLENTKTVSE FTLDSEIQTV KLINDTNLIV ATRTTLNAIN LLRGQVINSF DLYPFVNGVY KNGHMDRLIT CDERTGNI A LVINQQLTDL DGVPTINYKS RIIIFDSDLS TKLGNFTHHE YISWIGWNYD TDFIFLDIES TLGVVGTTVN TQLSDEVNN EGILDGLVSN TITTSASNSD IFAEQLHKLS SRGKKSDTRD KNTNDNDEDE EDIALEFING EKKDKLVNMN SFTSMFDNIQ NVQMDTFFD RVMKVLT

Macromolecule #16: U3 small nucleolar RNA-associated protein 18

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 18 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 66.49425 KDa

Sequence

String:

MTMATTAMNV SVPPPDEEEQ LLAKFVFGDT TDLQENLAKF NADFIFNEQE MDVEDQEDEG SESDNSEEDE AQNGELDHVN NDQLFFVDD GGNEDSQDKN EDTMDVDDED DSSSDDYSED SEEAAWIDSD DEKIKVPILV TNKTKKLRTS YNESKINGVH Y INRLRSQF EKIYPRPKWV DDESDSELDD EEDDEEEGSN NVINGDINAL TKILSTTYNY KDTLSNSKLL PPKKLDIVRL KD ANASHPS HSAIQSLSFH PSKPLLLTGG YDKTLRIYHI DGKTNHLVTS LHLVGSPIQT CTFYTSLSNQ NQQNIFTAGR RRY MHSWDL SLENLTHSQT AKIEKFSRLY GHESTQRSFE NFKVAHLQNS QTNSVHGIVL LQGNNGWINI LHSTSGLWLM GCKI EGVIT DFCIDYQPIS RGKFRTILIA VNAYGEVWEF DLNKNGHVIR RWKDQGGVGI TKIQVGGGTT TTCPALQISK IKQNR WLAV GSESGFVNLY DRNNAMTSST PTPVAALDQL TTTISNLQFS PDGQILCMAS RAVKDALRLV HLPSCSVFSN WPTSGT PLG KVTSVAFSPS GGLLAVGNEQ GKVRLWKLNH Y

Macromolecule #17: Nucleolar complex protein 4

• Macromolecule: Name: Nucleolar complex protein 4 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 63.707844 KDa

Sequence

String:

MVLLISEIKD IAKRLTAAGD RKQYNSIIKL INELVIPENV TQLEEDETEK NLRFLVMSLF QIFRKLFSRG DLTLPSSKKS TLEKEQFVN WCRKVYEAFK TKLLAIISDI PFETSLGLDS LDVYLQLAEL ESTHFASEKG APFFPNKTFR KLIIALWSSN M GEIEDVKS SGASENLIIV EFTEKYYTKF ADIQYYFQSE FNQLLEDPAY QDLLLKNVGK WLALVNHDKH CSSVDADLEI FV PNPPQAI ENESKFKSNF EKNWLSLLNG QLSLQQYKSI LLILHKRIIP HFHTPTKLMD FLTDSYNLQS SNKNAGVVPI LAL NGLFEL MKRFNLEYPN FYMKLYQIIN PDLMHVKYRA RFFRLMDVFL SSTHLSAHLV ASFIKKLARL TLESPPSAIV TVIP FIYNL IRKHPNCMIM LHNPAFISNP FQTPDQVANL KTLKENYVDP FDVHESDPEL THALDSSLWE LASLMEHYHP NVATL AKIF AQPFKKLSYN MEDFLDWNYD SLLNAESSRK LKTLPTLEFE AFTNVFDNED GDSEASSQGN VYLPGVAW

Macromolecule #18: U3 small nucleolar RNA-associated protein 20

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 20 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 287.9155 KDa

Sequence

String:

MAKQRQTTKS SKRYRYSSFK ARIDDLKIEP ARNLEKRVHD YVESSHFLAS FDQWKEINLS AKFTEFAAEI EHDVQTLPQI LYHDKKIFN SLVSFINFHD EFSLQPLLDL LAQFCHDLGP DFLKFYEEAI KTLINLLDAA IEFESSNVFE WGFNCLAYIF K YLSKFLVK KLVLTCDLLI PLLSHSKEYL SRFSAEALSF LVRKCPVSNL REFVRSVFEK LEGDDEQTNL YEGLLILFTE SM TSTQETL HSKAKAIMSV LLHEALTKSS PERSVSLLSD IWMNISKYAS IESLLPVYEV MYQDFNDSLD ATNIDRILKV LTT IVFSES GRKIPDWNKI TILIERIMSQ SENCASLSQD KVAFLFALFI RNSDVKTLTL FHQKLFNYAL TNISDCFLEF FQFA LRLSY ERVFSFNGLK FLQLFLKKNW QSQGKKIALF FLEVDDKPEL QKVREVNFPE EFILSIRDFF VTAEINDSND LFEIY WRAI IFKYSKLQNT EIIIPLLERI FSTFASPDNF TKDMVGTLLK IYRKEDDASG NNLLKTILDN YENYKESLNF LRGWNK LVS NLHPSESLKG LMSHYPSLLL SLTDNFMLPD GKIRYETLEL MKTLMILQGM QVPDLLSSCM VIEEIPLTLQ NARDLTI RI KNVGAEFGKT KTDKLVSSFF LKYLFGLLTV RFSPVWTGVF DTLPNVYTKD EALVWKLVLS FIKLPDENQN LDYYQPLL E DGANKVLWDS SVVRLRDTID TFSHIWSKYS TQNTSIISTT IERRGNTTYP ILIRNQALKV MLSIPQVAEN HFVDIAPFV YNDFKTYKDE EDMENERVIT GSWTEVDRNV FLKTLSKFKN IKNVYSATEL HDHLMVLLGS RNTDVQKLAL DALLAYKNPT LNKYRDNLK NLLDDTLFKD EITTFLTENG SQSIKAEDEK VVMPYVLRIF FGRAQVPPTS GQKRSRKIAV ISVLPNFKKP Y INDFLSLA SERLDYNYFF GNSHQINSSK ATLKTIRRMT GFVNIVNSTL SVLRTNFPLH TNSVLQPLIY SIAMAYYVLD TE STEEVHL RKMASNLRQQ GLKCLSSVFE FVGNTFDWST SMEDIYAVVV KPRISHFSDE NLQQPSSLLR LFLYWAHNPS LYQ FLYYDE FATATALMDT ISNQHVKEAV IGPIIEAADS IIRNPVNDDH YVDLVTLICT SCLKILPSLY VKLSDSNSIS TFLN LLVSI TEMGFIQDDH VRSRLISSLI SILKGKLKKL QENDTQKILK ILKLIVFNYN CSWSDIEELY TTISSLFKTF DERNL RVSL TELFIELGRK VPELESISKL VADLNSYSSS RMHEYDFPRI LSTFKGLIED GYKSYSELEW LPLLFTFLHF INNKEE LAL RTNASHAIMK FIDFINEKPN LNEASKSISM LKDILLPNIR IGLRDSLEEV QSEYVSVLSY MVKNTKYFTD FEDMAIL LY NGDEEADFFT NVNHIQLHRR QRAIKRLGEH AHQLKDNSIS HYLIPMIEHY VFSDDERYRN IGNETQIAIG GLAQHMSW N QYKALLRRYI SMLKTKPNQM KQAVQLIVQL SVPLRETLRI VRDGAESKLT LSKFPSNLDE PSNFIKQELY PTLSKILGT RDDETIIERM PIAEALVNIV LGLTNDDITN FLPSILTNIC QVLRSKSEEL RDAVRVTLGK ISIILGAEYL VFVIKELMAT LKRGSQIHV LSYTVHYILK SMHGVLKHSD LDTSSSMIVK IIMENIFGFA GEEKDSENYH TKVKEIKSNK SYDAGEILAS N ISLTEFGT LLSPVKALLM VRINLRNQNK LSELLRRYLL GLNHNSDSES ESILKFCHQL FQESEMSNSP QIPKKKVKDQ VD EKEDFFL VNLESKSYTI NSNSLLLNST LQKFALDLLR NVITRHRSFL TVSHLEGFIP FLRDSLLSEN EGVVISTLRI LIT LIRLDF SDESSEIFKN CARKVLNIIK VSPSTSSELC QMGLKFLSAF IRHTDSTLKD TALSYVLGRV LPDLNEPSRQ GLAF NFLKA LVSKHIMLPE LYDIADTTRE IMVTNHSKEI RDVSRSVYYQ FLMEYDQSKG RLEKQFKFMV DNLQYPTESG RQSVM ELIN LIITKANPAL LSKLSSSFFL ALVNVSFNDD APRCREMASV LISTMLPKLE NKDLEIVEKY IAAWLKQVDN ASFLNL GLR TYKVYLKSIG FEHTIELDEL AIKRIRYILS DTSVGSEHQW DLVYSALNTF SSYMEATESV YKHGFKDIWD GIITCLL YP HSWVRQSAAN LVHQLIANKD KLEISLTNLE IQTIATRILH QLGAPSIPEN LANVSIKTLV NISILWKEQR TPFIMDVS K QTGEDLKYTT AIDYMVTRIG GIIRSDEHRM DSFMSKKACI QLLALLVQVL DEDEVIAEGE KILLPLYGYL ETYYSRAVD EEQEELRTLS NECLKILEDK LQVSDFTKIY TAVKQTVLER RKERRSKRAI LAVNAPQISA DKKLRKHARS REKRKHEKDE NGYYQRRNK RKRA

Macromolecule #19: U3 small nucleolar RNA-associated protein 21

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 104.927844 KDa

Sequence

String:

MSIDLKKRKV EEDVRSRGKN SKIFSPFRII GNVSNGVPFA TGTLGSTFYI VTCVGKTFQI YDANTLHLLF VSEKETPSSI VALSAHFHY VYAAYENKVG IYKRGIEEHL LELETDANVE HLCIFGDYLC ASTDDNSIFI YKKSDPQDKY PSEFYTKLTV T EIQGGEIV SLQHLATYLN KLTVVTKSNV LLFNVRTGKL VFTSNEFPDQ ITTAEPAPVL DIIALGTVTG EVIMFNMRKG KR IRTIKIP QSRISSLSFR TDGSSHLSVG TSSGDLIFYD LDRRSRIHVL KNIHRESYGG VTQATFLNGQ PIIVTSGGDN SLK EYVFDP SLSQGSGDVV VQPPRYLRSR GGHSQPPSYI AFADSQSHFM LSASKDRSLW SFSLRKDAQS QEMSQRLHKK QDGG RVGGS TIKSKFPEIV ALAIENARIG EWENIITAHK DEKFARTWDM RNKRVGRWTF DTTDDGFVKS VAMSQCGNFG FIGSS NGSI TIYNMQSGIL RKKYKLHKRA VTGISLDGMN RKMVSCGLDG IVGFYDFNKS TLLGKLKLDA PITAMVYHRS SDLFAL ALD DLSIVVIDAV TQRVVRQLWG HSNRITAFDF SPEGRWIVSA SLDSTIRTWD LPTGGCIDGI IVDNVATNVK FSPNGDL LA TTHVTGNGIC IWTNRAQFKT VSTRTIDESE FARMALPSTS VRGNDSMLSG ALESNGGEDL NDIDFNTYTS LEQIDKEL L TLSIGPRSKM NTLLHLDVIR KRSKPKEAPK KSEKLPFFLQ LSGEKVGDEA SVREGIAHET PEEIHRRDQE AQKKLDAEE QMNKFKVTGR LGFESHFTKQ LREGSQSKDY SSLLATLINF SPAAVDLEIR SLNSFEPFDE IVWFIDALTQ GLKSNKNFEL YETFMSLLF KAHGDVIHAN NKNQDIASAL QNWEDVHKKE DRLDDLVKFC MGVAAFVTTA

Macromolecule #20: U3 small nucleolar RNA-associated protein 22

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 22 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 140.660141 KDa

Sequence

String:

MATSVKRKAS ETSDQNIVKV QKKHSTQDST TDNGSKENDH SSQAINERTV PEQENDESDT SPESNEVATN TAATRHNGKV TATESYDIH IARETAELFK SNIFKLQIDE LLEQVKLKQK HVLKVEKFLH KLYDILQEIP DWEEKSLAEV DSFFKNKIVS V PFVDPKPI PQNTNYKFNY KKPDISLIGS FALKAGIYQP NGSSIDTLLT MPKELFEKKD FLNFRCLHKR SVYLAYLTHH LL ILLKKDK LDSFLQLEYS YFDNDPLLPI LRISCSKPTG DSLSDYNFYK TRFSINLLIG FPYKVFEPKK LLPNRNCIRI AQE SKEQSL PATPLYNFSV LSSSTHENYL KYLYKTKKQT ESFVEATVLG RLWLQQRGFS SNMSHSGSLG GFGTFEFTIL MAAL LNGGG INSNKILLHG FSSYQLFKGV IKYLATMDLC HDGHLQFHSN PENSSSSPAS KYIDEGFQTP TLFDKSTKVN ILTKM TVSS YQILKEYAGE TLRMLNNVVQ DQFSNIFLTN ISRFDNLKYD LCYDVQLPLG KYNNLETSLA ATFGSMERVK FITLEN FLA HKITNVARYA LGDRIKYIQI EMVGQKSDFP ITKRKVYSNT GGNHFNFDFV RVKLIVNPSE CDKLVTKGPA HSETMST EA AVFKNFWGIK SSLRRFKDGS ITHCCVWSTS SSEPIISSIV NFALQKHVSK KAQISNETIK KFHNFLPLPN LPSSAKTS V LNLSSFFNLK KSFDDLYKII FQMKLPLSVK SILPVGSAFR YTSLCQPVPF AYSDPDFFQD VILEFETSPK WPDEITSLE KAKTAFLLKI QEELSANSST YRSFFSRDES IPYNLEIVTL NILTPEGYGF KFRVLTERDE ILYLRAIANA RNELKPELEA TFLKFTAKY LASVRHTRTL ENISHSYQFY SPVVRLFKRW LDTHLLLGHI TDELAELIAI KPFVDPAPYF IPGSLENGFL K VLKFISQW NWKDDPLILD LVKPEDDIRD TFETSIGAGS ELDSKTMKKL SERLTLAQYK GIQMNFTNLR NSDPNGTHLQ FF VASKNDP SGILYSSGIP LPIATRLTAL AKVAVNLLQT HGLNQQTINL LFTPGLKDYD FVVDLRTPIG LKSSCGILSA TEF KNITND QAPSNFPENL NDLSEKMDPT YQLVKYLNLK YKNSLILSSR KYIGVNGGEK GDKNVITGLI KPLFKGAHKF RVNL DCNVK PVDDENVILN KEAIFHEIAA FGNDMVINFE TD

Macromolecule #21: rRNA-processing protein FCF1

• Macromolecule: Name: rRNA-processing protein FCF1 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.650729 KDa

Sequence

String:

MGKAKKTRKF GLVKRTLNTK KDQRLKKNQE NIKTKEDPEL TRNIPQVSSA LFFQYNQAIK PPYQVLIDTN FINFSIQKKV DIVRGMMDC LLAKCNPLIT DCVMAELEKL GPKYRIALKL ARDPRIKRLS CSHKGTYADD CLVHRVLQHK CYIVATNDAG L KQRIRKIP GIPLMSVGGH AYVIEKLPDV F

Macromolecule #22: rRNA 2'-O-methyltransferase fibrillarin

• Macromolecule: Name: rRNA 2'-O-methyltransferase fibrillarin / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO; EC number: Transferases; Transferring one-carbon groups; Methyltransferases
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 34.525418 KDa

Sequence

String:

MSFRPGSRGG SRGGSRGGFG GRGGSRGGAR GGSRGGFGGR GGSRGGARGG SRGGFGGRGG SRGGARGGSR GGRGGAAGGA RGGAKVVIE PHRHAGVYIA RGKEDLLVTK NMAPGESVYG EKRISVEEPS KEDGVPPTKV EYRVWNPFRS KLAAGIMGGL D ELFIAPGK KVLYLGAASG TSVSHVSDVV GPEGVVYAVE FSHRPGRELI SMAKKRPNII PIIEDARHPQ KYRMLIGMVD CV FADVAQP DQARIIALNS HMFLKDQGGV VISIKANCID STVDAETVFA REVQKLREER IKPLEQLTLE PYERDHCIVV GRY MRSGLK K

Macromolecule #23: Nucleolar protein 56

• Macromolecule: Name: Nucleolar protein 56 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 56.961152 KDa

Sequence

String:

MAPIEYLLFE EPTGYAVFKV KLQQDDIGSR LKEVQEQIND FGAFTKLIEL VSFAPFKGAA EALENANDIS EGLVSESLKA ILDLNLPKA SSKKKNITLA ISDKNLGPSI KEEFPYVDCI SNELAQDLIR GVRLHGEKLF KGLQSGDLER AQLGLGHAYS R AKVKFSVQ KNDNHIIQAI ALLDQLDKDI NTFAMRVKEW YGWHFPELAK LVPDNYTFAK LVLFIKDKAS LNDDSLHDLA AL LNEDSGI AQRVIDNARI SMGQDISETD MENVCVFAQR VASLADYRRQ LYDYLCEKMH TVAPNLSELI GEVIGARLIS HAG SLTNLS KQAASTVQIL GAEKALFRAL KTKGNTPKYG LIYHSGFISK ASAKNKGRIS RYLANKCSMA SRIDNYSEEP SNVF GSVLK KQVEQRLEFY NTGKPTLKNE LAIQEAMELY NKDKPAAEVE ETKEKESSKK RKLEDDDEEK KEKKEKKSKK EKKEK KEKK DKKEKKDKKE KKDKKKKSKD

Macromolecule #24: Nucleolar protein 58

• Macromolecule: Name: Nucleolar protein 58 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 57.060344 KDa

Sequence

String:

MAYVLTETSA GYALLKASDK KIYKSSSLIQ DLDSSDKVLK EFKIAAFSKF NSAANALEEA NSIIEGKVSS QLEKLLEEIK KDKKSTLIV SETKLANAIN KLGLNFNVVS DAVTLDIYRA IKEYLPELLP GMSDNDLSKM SLGLAHSIGR HKLKFSADKV D VMIIQAIA LLDDLDKELN TYAMRCKEWY GWHFPELAKI VTDSVAYARI ILTMGIRSKA SETDLSEILP EEIEERVKTA AE VSMGTEI TQTDLDNINA LAEQIVEFAA YREQLSNYLS ARMKAIAPNL TQLVGELVGA RLIAHSGSLI SLAKSPASTI QIL GAEKAL FRALKTKHDT PKYGLLYHAS LVGQATGKNK GKIARVLAAK AAVSLRYDAL AEDRDDSGDI GLESRAKVEN RLSQ LEGRD LRTTPKVVRE AKKVEMTEAR AYNADADTAK AASDSESDSD DEEEEKKEKK EKKRKRDDDE DSKDSKKAKK EKKDK KEKK EKKEKKEKKE KKEKKEKKSK KEKKEKK

Macromolecule #25: 13 kDa ribonucleoprotein-associated protein

• Macromolecule: Name: 13 kDa ribonucleoprotein-associated protein / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.582855 KDa

Sequence

String:

MSAPNPKAFP LADAALTQQI LDVVQQAANL RQLKKGANEA TKTLNRGISE FIIMAADCEP IEILLHLPLL CEDKNVPYVF VPSRVALGR ACGVSRPVIA ASITTNDASA IKTQIYAVKD KIETLLI

Macromolecule #26: Ribosomal RNA-processing protein 9

• Macromolecule: Name: Ribosomal RNA-processing protein 9 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 65.146969 KDa

Sequence

String:

MSDVTQQKKR KRSKGEVNPS KPTVDEEITD PSSNEDEQLE VSDEEDALES EEEFEGENPA DKRRRLAKQY LENLKSEAND ILTDNRNAE EKDLNNLKER TIDEYNNFDA GDLDKDIIAS RLKEDVAEQQ GRVFRYFGDK LLISEAKQSF TRVGENNLTC I SCFQPVLN KYTFEESSNG DKNKGRLFAY TVSKDLQLTK YDITDFSKRP KKLKYAKGGA KYIPTSKHEY ENTTEGHYDE IL TVAASPD GKYVVTGGRD RKLIVWSTES LSPVKVIPTK DRRGEVLSLA FRKNSDQLYA SCADFKIRTY SINQFSQLEI LYG HHDIVE DISALAMERC VTVGARDRTA MLWKIPDETR LTFRGGDEPQ KLLRRWMKEN AKEGEDGEVK YPDESEAPLF FCEG SIDVV SMVDDFHFIT GSDNGNICLW SLAKKKPIFT ERIAHGILPE PSFNDISGET DEELRKRQLQ GKKLLQPFWI TSLYA IPYS NVFISGSWSG SLKVWKISDN LRSFELLGEL SGAKGVVTKI QVVESGKHGK EKFRILASIA KEHRLGRWIA NVSGAR NGI YSAVIDQTGF

Macromolecule #27: U3 small nucleolar ribonucleoprotein protein IMP3

• Macromolecule: Name: U3 small nucleolar ribonucleoprotein protein IMP3 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.928529 KDa

Sequence

String:

MVRKLKHHEQ KLLKKVDFLE WKQDQGHRDT QVMRTYHIQN REDYHKYNRI CGDIRRLANK LSLLPPTDPF RRKHEQLLLD KLYAMGVLT TKSKISDLEN KVTVSAICRR RLPVIMHRLK MAETIQDAVK FIEQGHVRVG PNLINDPAYL VTRNMEDYVT W VDNSKIKK TLLRYRNQID DFDFS

Macromolecule #28: U3 small nucleolar ribonucleoprotein protein IMP4

• Macromolecule: Name: U3 small nucleolar ribonucleoprotein protein IMP4 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 33.536168 KDa

Sequence

String:

MLRRQARERR EYLYRKAQEL QDSQLQQKRQ IIKQALAQGK PLPKELAEDE SLQKDFRYDQ SLKESEEADD LQVDDEYAAT SGIMDPRII VTTSRDPSTR LSQFAKEIKL LFPNAVRLNR GNYVMPNLVD ACKKSGTTDL VVLHEHRGVP TSLTISHFPH G PTAQFSLH NVVMRHDIIN AGNQSEVNPH LIFDNFTTAL GKRVVCILKH LFNAGPKKDS ERVITFANRG DFISVRQHVY VR TREGVEI AEVGPRFEMR LFELRLGTLE NKDADVEWQL RRFIRTANKK DYL

Macromolecule #29: U3 small nucleolar RNA-associated protein MPP10

• Macromolecule: Name: U3 small nucleolar RNA-associated protein MPP10 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 67.042492 KDa

Sequence

String:

MSELFGVLKS NAGRIILKDP SATSKDVKAY IDSVINTCKN GSITKKAELD EITVDGLDAN QVWWQVKLVL DSIDGDLIQG IQELKDVVT PSHNLSDGST LNSSSGEESE LEEAESVFKE KQMLSADVSE IEEQSNDSLS ENDEEPSMDD EKTSAEAARE E FAEEKRIS SGQDERHSSP DPYGINDKFF DLEKFNRDTL AAEDSNEASE GSEDEDIDYF QDMPSDDEEE EAIYYEDFFD KP TKEPVKK HSDVKDPKED EELDEEEHDS AMDKVKLDLF ADEEDEPNAE GVGEASDKNL SSFEKQQIEI RKQIEQLENE AVA EKKWSL KGEVKAKDRP EDALLTEELE FDRTAKPVPV ITSEVTESLE DMIRRRIQDS NFDDLQRRTL LDITRKSQRP QFEL SDVKS SKSLAEIYED DYTRAEDESA LSEELQKAHS EISELYANLV YKLDVLSSVH FVPKPASTSL EIRVETPTIS MEDAQ PLYM SNASSLAPQE IYNVGKAEKD GEIRLKNGVA MSKEELTRED KNRLRRALKR KRSKANLPNV NKRSKRNDVV DTLSKA KNI TVINQKGEKK DVSGKTKKSR SGPDSTNIKL

Macromolecule #30: Ribosome biogenesis protein BMS1

• Macromolecule: Name: Ribosome biogenesis protein BMS1 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 135.792281 KDa

Sequence

String:

MEQSNKQHRK AKEKNTAKKK LHTQGHNAKA FAVAAPGKMA RTMQRSSDVN ERKLHVPMVD RTPEDDPPPF IVAVVGPPGT GKTTLIRSL VRRMTKSTLN DIQGPITVVS GKHRRLTFLE CPADDLNAMI DIAKIADLVL LLIDGNFGFE METMEFLNIA Q HHGMPRVL GVATHLDLFK SQSTLRASKK RLKHRFWTEV YQGAKLFYLS GVINGRYPDR EILNLSRFIS VMKFRPLKWR NE HPYMLAD RFTDLTHPEL IETQGLQIDR KVAIYGYLHG TPLPSAPGTR VHIAGVGDFS VAQIEKLPDP CPTPFYQQKL DDF EREKMK EEAKANGEIT TASTTRRRKR LDDKDKLIYA PMSDVGGVLM DKDAVYIDIG KKNEEPSFVP GQERGEGEKL MTGL QSVEQ SIAEKFDGVG LQLFSNGTEL HEVADHEGMD VESGEESIED DEGKSKGRTS LRKPRIYGKP VQEEDADIDN LPSDE EPYT NDDDVQDSEP RMVEIDFNNT GEQGAEKLAL ETDSEFEESE DEFSWERTAA NKLKKTESKK RTWNIGKLIY MDNISP EEC IRRWRGEDDD SKDESDIEED VDDDFFRKKD GTVTKEGNKD HAVDLEKFVP YFDTFEKLAK KWKSVDAIKE RFLGAGI LG NDNKTKSDSN EGGEELYGDF EDLEDGNPSE QAEDNSDKES EDEDENEDTN GDDDNSFTNF DAEEKKDLTM EQEREMNA A KKEKLRAQFE IEEGENFKED DENNEYDTWY ELQKAKISKQ LEINNIEYQE MTPEQRQRIE GFKAGSYVRI VFEKVPMEF VKNFNPKFPI VMGGLLPTEI KFGIVKARLR RHRWHKKILK TNDPLVLSLG WRRFQTLPIY TTTDSRTRTR MLKYTPEHTY CNAAFYGPL CSPNTPFCGV QIVANSDTGN GFRIAATGIV EEIDVNIEIV KKLKLVGFPY KIFKNTAFIK DMFSSAMEVA R FEGAQIKT VSGIRGEIKR ALSKPEGHYR AAFEDKILMS DIVILRSWYP VRVKKFYNPV TSLLLKEKTE WKGLRLTGQI RA AMNLETP SNPDSAYHKI ERVERHFNGL KVPKAVQKEL PFKSQIHQMK PQKKKTYMAK RAVVLGGDEK KARSFIQKVL TIS KAKDSK RKEQKASQRK ERLKKLAKME EEKSQRDKEK KKEYFAQNGK RTTMGGDDES RPRKMRR

Macromolecule #31: RNA 3'-terminal phosphate cyclase-like protein

• Macromolecule: Name: RNA 3'-terminal phosphate cyclase-like protein / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 40.220559 KDa

Sequence

String:

MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHI CPSSKPVGYF VEPMLYLAPF SKKKFSILFK GITASHNDAG IEAIKWGLMP VMEKFGVREC ALHTLKRGSP P LGGGEVHL VVDSLIAQPI TMHEIDRPII SSITGVAYST RVSPSLVNRM IDGAKKVLKN LQCEVNITAD VWRGENSGKS PG WGITLVA QSKQKGWSYF AEDIGDAGSI PEELGEKVAC QLLEEISKSA AVGRNQLPLA IVYMVIGKED IGRLRINKEQ IDE RFIILL RDIKKIFNTE VFLKPVDEAD NEDMIATIKG IGFTNTSKKI A

Macromolecule #32: Ribosomal RNA-processing protein 7

• Macromolecule: Name: Ribosomal RNA-processing protein 7 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 34.526441 KDa

Sequence

String:

MGIEDISAMK NGFIVVPFKL PDHKALPKSQ EASLHFMFAK RHQSSNSNES DCLFLVNLPL LSNIEHMKKF VGQLCGKYDT VSHVEELLY NDEFGLHEVD LSALTSDLMS STDVNEKRYT PRNTALLKFV DAASINNCWN ALKKYSNLHA KHPNELFEWT Y TTPSFTTF VNFYKPLDID YLKEDIHTHM AIFEQREAQA QEDVQSSIVD EDGFTLVVGK NTKSLNSIRK KILNKNPLSK HE NKAKPIS NIDKKAKKDF YRFQVRERKK QEINQLLSKF KEDQERIKVM KAKRKFNPYT

Macromolecule #33: Probable ATP-dependent RNA helicase DHR1

• Macromolecule: Name: Probable ATP-dependent RNA helicase DHR1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 145.171406 KDa

Sequence

String:

MGTYRKRFNE KARSGHMAKL KELKRIRNKQ FTRQDENDER VENPDSAPAE SSTTEPNANA EILEPLTEEE KKMKKRKLQE LFTPKESKV SRLKKKRLDK FIEHQLKREE RKTIIGKLQD YKIDTSLLTS SKRLGEGRQT KKEEFKEALS LERQGRGNEQ T NEILYEEY EPKVWDEYGE GGSSEDDDGE DDFEASFGSM PKPTDNEEKK SSGFIDHRPA KFGGSGLSFG FSNIKVINKE SK TPKKKYN WRQRVEMEEL KKHGKEDEMD FDTTSEDDDE EEDQEEEDKM HPSENPLEEV ESADSETGSE KFDQNDVANE FKD WANQEI KKLEGRDQEL VTPTLNIDYK PIIRKEDLDD GLQEAYVPIN ENSTRKAFYV EVSRSDEIQK ARIQLPVFGE EHKI MEAIH HNDVVIICGE TGSGKTTQVP QFLYEAGFGA EDSPDYPGMV GITQPRRVAA VSMAERVANE LGDHGHKVGY QIRFD STAK EDTKVKFMTD GVLLREMMHD FKLTKYSSII IDEAHERNIN TDILIGMLSR CVRLRAKLHK ENPIEHKKLK LIIMSA TLR VSDFSENKTL FPIAPPVLQV DARQFPVSIH FNRRTAFNYT DEAFRKTCKI HQKLPPGAIL VFLTGQQEIT HMVKRLR KE FPFKKNSKYN KDLETPVSKM GINSKTTDLE AEDIDFSVQV IDQDKFKSAI RYEEDEGNSG NGEDEEDEEE EGFEEVLT E GQTANDPLYV LPLYSLLPTK EQMRVFQKPP QGSRLCIVAT NVAETSLTIP GVRYVVDSGR SKERKYNESN GVQSFEVGW VSKASANQRS GRAGRTGPGH CYRLYSSAVF EHDFEQFSKP EILRMPVESI VLQMKSMAIH NIINFPFPTP PDRVALSKAI QLLQYLGAL DNKEMITEDG KKMSLFPLSP RFSKMLLVSD EKACLPYIVA IVSALSVGDP FINEFELGIN EISRKPNPDE N LDDKIREH DESTPGMDPE LKKELRSKFY KSRSQFSKLD KFSDVFRLLS VVSAMDYVPK EQKEIFMKKN FLRGKLMEEI VK LRKQLMY IIKSNTSKEN IAVVIRNEDL KSDIPSVIQI KLLKQMICAG FVDHVAVRAD VLFPDDAKIT NRTSIINIPY IPV LATRTP NIEDCFVYIH PTSILNNLGE MPPKYMLYYS LHLGGNNKTR MNTLCDIAST PLANIARKGL LLTYSKPLTG QGLK TVNLS PTERYCYVVP RFGSTVDNDL KIGWDLNPIA VHQKKQKGQW TVIKFITRKG FQTITGEEKE KK

Macromolecule #34: Ribosomal RNA small subunit methyltransferase NEP1

• Macromolecule: Name: Ribosomal RNA small subunit methyltransferase NEP1 / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO; EC number: rRNA small subunit pseudouridine methyltransferase Nep1
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.936461 KDa

Sequence

String:

MVEDSRVRDA LKGGDQKALP ASLVPQAPPV LTSKDKITKR MIVVLAMASL ETHKISSNGP GGDKYVLLNC DDHQGLLKKM GRDISEARP DITHQCLLTL LDSPINKAGK LQVYIQTSRG ILIEVNPTVR IPRTFKRFSG LMVQLLHKLS IRSVNSEEKL L KVIKNPIT DHLPTKCRKV TLSFDAPVIR VQDYIEKLDD DESICVFVGA MARGKDNFAD EYVDEKVGLS NYPLSASVAC SK FCHGAED AWNIL

Macromolecule #35: Essential nuclear protein 1

• Macromolecule: Name: Essential nuclear protein 1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 55.207422 KDa

Sequence

String:

MARASSTKAR KQRHDPLLKD LDAAQGTLKK INKKKLAQND AANHDAANEE DGYIDSKASR KILQLAKEQQ DEIEGEELAE SERNKQFEA RFTTMSYDDE DEDEDEDEEA FGEDISDFEP EGDYKEEEEI VEIDEEDAAM FEQYFKKSDD FNSLSGSYNL A DKIMASIR EKESQVEDMQ DDEPLANEQN TSRGNISSGL KSGEGVALPE KVIKAYTTVG SILKTWTHGK LPKLFKVIPS LR NWQDVIY VTNPEEWSPH VVYEATKLFV SNLTAKESQK FINLILLERF RDNIETSEDH SLNYHIYRAV KKSLYKPSAF FKG FLFPLV ETGCNVREAT IAGSVLAKVS VPALHSSAAL SYLLRLPFSP PTTVFIKILL DKKYALPYQT VDDCVYYFMR FRIL DDGSN GEDATRVLPV IWHKAFLTFA QRYKNDITQD QRDFLLETVR QRGHKDIGPE IRRELLAGAS REFVDPQEAN DDLMI DVN

Macromolecule #36: Dimethyladenosine transferase

• Macromolecule: Name: Dimethyladenosine transferase / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO; EC number: 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 36.003836 KDa

Sequence

String:

MGKAAKKKYS GATSSKQVSA EKHLSSVFKF NTDLGQHILK NPLVAQGIVD KAQIRPSDVV LEVGPGTGNL TVRILEQAKN VVAVEMDPR MAAELTKRVR GTPVEKKLEI MLGDFMKTEL PYFDICISNT PYQISSPLVF KLINQPRPPR VSILMFQREF A LRLLARPG DSLYCRLSAN VQMWANVTHI MKVGKNNFRP PPQVESSVVR LEIKNPRPQV DYNEWDGLLR IVFVRKNRTI SA GFKSTTV MDILEKNYKT FLAMNNEMVD DTKGSMHDVV KEKIDTVLKE TDLGDKRAGK CDQNDFLRLL YAFHQVGIHF S

Macromolecule #37: rRNA-processing protein FCF2

• Macromolecule: Name: rRNA-processing protein FCF2 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.68924 KDa

Sequence

String:

MDQSVEDLFG ALRDASASLE VKNSAKEQVS LQQEDVLQIG NNDDEVEIES KFQEIETNLK KLPKLETGFD ALANKKKKKN VLPSVETED KRKPNKSDKN DNDWFTLPKP DDNMRREVQR DLLLIKHRAA LDPKRHYKKQ RWEVPERFAI GTIIEDKSEF Y SSRMNRKE RKSTILETLM GDEASNKYFK RKYNEIQEKS TSGRKAHYKK MKEMRKKRR

Macromolecule #38: 40S ribosomal protein S27-A

• Macromolecule: Name: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 8.893391 KDa

Sequence

String:

MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK

Macromolecule #39: Pre-rRNA-processing protein PNO1

• Macromolecule: Name: Pre-rRNA-processing protein PNO1 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 30.380623 KDa

Sequence

String:

MVAPTALKKA TVTPVSGQDG GSSRIIGINN TESIDEDDDD DVLLDDSDNN TAKEEVEGEE GSRKTHESKT VVVDDQGKPR FTSASKTQG NKIKFESRKI MVPPHRMTPL RNSWTKIYPP LVEHLKLQVR MNLKTKSVEL RTNPKFTTDP GALQKGADFI K AFTLGFDL DDSIALLRLD DLYIETFEVK DVKTLTGDHL SRAIGRIAGK DGKTKFAIEN ATRTRIVLAD SKIHILGGFT HI RMARESV VSLILGSPPG KVYGNLRTVA SRLKERY

Macromolecule #40: 40S ribosomal protein S1-A

• Macromolecule: Name: 40S ribosomal protein S1-A / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 28.798467 KDa

Sequence

String:

MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI SEILTKEVQG STLAQLTSKL IPEVINKEIE NATKDIFPLQ NIHVRKVKLL KQPKFDVGAL MALHGEGSGE EK GKKVTGF KDEVLETV

Macromolecule #41: 40S ribosomal protein S4-A

• Macromolecule: Name: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 29.46933 KDa

Sequence

String:

MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ITDFIKFDAG KLVYVTGGRN LGRIGTIVHK ERHDGGFDLV HIKDSLDNTF VTRLNNVFVI GEQGKPYISL PK GKGIKLS IAEERDRRRA QQGL

Macromolecule #42: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.0726 KDa

Sequence

String:

MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV NQAIALLTIG AREAAFRNIK TIAETLAEEL INAAKGSSTS YAIKKKDELE RVAKSNR

Macromolecule #43: 40S ribosomal protein S6-A

• Macromolecule: Name: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.054486 KDa

Sequence

String:

MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK RRASSLKA

Macromolecule #44: 40S ribosomal protein S7-A

• Macromolecule: Name: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.658209 KDa

Sequence

String:

MSAPQAKILS QAPTELELQV AQAFVELENS SPELKAELRP LQFKSIREID VAGGKKALAI FVPVPSLAGF HKVQTKLTRE LEKKFQDRH VIFLAERRIL PKPSRTSRQV QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKVLLDSKDV Q QIDYKLES FQAVYNKLTG KQIVFEIPSE TH

Macromolecule #45: 40S ribosomal protein S8-A

• Macromolecule: Name: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.537803 KDa

Sequence

String:

MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

Macromolecule #46: 40S ribosomal protein S9-A

• Macromolecule: Name: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.487893 KDa

Sequence

String:

MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR PGRVARRNAA RKAEASGEAA DEADEADEE

Macromolecule #47: 40S ribosomal protein S11-A

• Macromolecule: Name: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.785934 KDa

Sequence

String:

MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTKM HRTIVIRRA YLHYIPKYNR YEKRHKNVPV HVSPAFRVQV GDIVTVGQCR PISKTVRFNV VKVSAAAGKA NKQFAKF

Macromolecule #48: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.059945 KDa

Sequence

String:

MGRMHSAGKG ISSSAIPYSR NAPAWFKLSS ESVIEQIVKY ARKGLTPSQI GVLLRDAHGV TQARVITGNK IMRILKSNGL APEIPEDLY YLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVAVLPPNW KYESATASAL VN

Macromolecule #49: 40S ribosomal protein S14-A

• Macromolecule: Name: 40S ribosomal protein S14-A / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.562655 KDa

Sequence

String:

MSNVVQARDN SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGT RTKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

Macromolecule #50: 40S ribosomal protein S16-A

• Macromolecule: Name: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.87749 KDa

Sequence

String:

MSAVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVE PEILRFKVYE PLLLVGLDKF SNIDIRVRVT GGGHVSQVYA IRQAIAKGL VAYHQKYVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGKGARSRFQ KSYR

Macromolecule #51: 40S ribosomal protein S18-A

• Macromolecule: Name: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.071641 KDa

Sequence

String:

MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ NDITDGKDYH TLANNVESKL RDDLERLKKI RAHRGIRHFW GLRVRGQHTK TTGRRRA

Macromolecule #52: 40S ribosomal protein S19-A

• Macromolecule: Name: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.942125 KDa

Sequence

String:

MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVR PYKHIDASGS INRKVLQALE KIGIVEISPK GGRRISENGQ RDLDRIAAQT LEEDE

Macromolecule #53: 40S ribosomal protein S22-A

• Macromolecule: Name: 40S ribosomal protein S22-A / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 14.650062 KDa

Sequence

String:

MTRSSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIGDIEKW TANLLPARQF GYVILTTSAG IMDHEEARRK HVSGKILGFV Y

Macromolecule #54: 40S ribosomal protein S23-A

• Macromolecule: Name: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.073896 KDa

Sequence

String:

MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK GIVLEKLGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

Macromolecule #55: 40S ribosomal protein S24-A

• Macromolecule: Name: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.362848 KDa

Sequence

String:

MSDAVTIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEVYKA EKDAVSVFGF RTQFGGGKSV GFGLVYNSVA EAKKFEPTY RLVRYGLAEK VEKASRQQRK QKKNRDKKIF GTGKRLAKKV ARRNAD

Macromolecule #56: 40S ribosomal protein S28-A

• Macromolecule: Name: 40S ribosomal protein S28-A / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 7.605847 KDa

Sequence

String:

MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE NDILVLMESE REARRLR

Macromolecule #57: 5ETS RNA

• Macromolecule: Name: 5ETS RNA / type: rna / ID: 57 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 26.247596 KDa

Sequence

String:

UGCGAAAGCA GUUGAAGACA AGUGCUUGUC GUUCGUUAAA AUGGCCUCGU CGGAUUUGGU GGAAGGAAAC UCAAAGAGUG C

Macromolecule #58: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 58 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 580.404438 KDa

Sequence

String:

UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCCGAU UUUUUCGUGU ACUGGAUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUG U GGCUCUUGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGUAU UGCUCGAAUA UAUUAGCAU GGAAUAAUAG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUU CAAUUGUCAG AGGUGAAAUU CUUGGAUUUA UUGAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU U UCAUUAAU CAAGAACGAA AGUUAGGGGA UCGAAGAUGA UCAGAUACCG UCGUAGUCUU AACCAUAAAC UAUGCCGACU AG GGAUCGG GUGGUGUUUU UUUAAUGACC CACUCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGU CGCAAG GCUAAAACUU AAAGGAAUUG ACGGAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGG GAAAC UCACCAGGUC CAGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCC GUUC UUAGUUGGUG GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCA UUU GCUGGUUAUC CACUUCUUAG AGGGACUAUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACAGGUCUG UGAUGCC CU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUU G UGAAACUCCG UCGUGCUGGG GAUAGAGCAU UGUAAUUAUU GCUCUUCAAC GAGGAAUUCC UAGUAAGCGC AAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUGAA UGGCUUAGUG AGGCCUCAGG AUCUGCUUA GAGAAGGGGG CAACUCCAUC UCAGAGCGGA GAAUUUGGAC AAACUUGGUC AUUUAGAGGA ACUAAAAGUC G UAACAAGG UUUCCGUAGG UGAACCUGCG GAAGGAUCAU UAAA

Macromolecule #59: U3 snoRNA

• Macromolecule: Name: U3 snoRNA / type: rna / ID: 59 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 106.542297 KDa

Sequence

String:

GUCGACGUAC UUCAUAGGAU CAUUUCUAUA GGAAUCGUCA CUCUUUGACU CUUCAAAAGA GCCACUGAAU CCAACUUGGU UGAUGAGUC CCAUAACCUU UGUACCCCAG AGUGAGAAAC CGAAAUUGAA UCUAAAUUAG CUUGGUCCGC AAUCCUUAGC G GUUCGGCC AUCUAUAAUU UUGAAUAAAA AUUUUGCUGU GCCGUUGCAU UUGUAGUUUU UUCCUUUGGA AGUAAUUACA AU AUUUUAU GGCGCGAUGA UCUUGACCCA UCCUAUGUAC UUCUUUUUUG AAGGGAUAGG GCUCUAUGGG UGGGUACAAA UGG CAGUCU GACAAGU

Macromolecule #60: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 60 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: Quantifoil / Material: COPPER / Support film - Material: CARBON
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 44.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Initial angle assignment: Type: OTHER / Details: Relion
• Final angle assignment: Type: OTHER / Details: Relion
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 7.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 11312

Atomic model buiding 1

• Refinement: Space: REAL

Output model

PDB-6zqe:
Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Dis-A (Poly-Ala)