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- EMDB-0952: Cryo-EM structure of 90S small subunit preribosomes in transition... -

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Basic information

Entry
Database: EMDB / ID: EMD-0952
TitleCryo-EM structure of 90S small subunit preribosomes in transition states (State D)
Map data
Sample90S pre-ribosome (Dhr1-depleted, state D)
  • (nucleic-acidNucleic acid) x 3
  • (40S ribosomal protein ...) x 16
  • rRNA 2'-O-methyltransferase fibrillarin
  • (Nucleolar protein ...Nucleolus) x 2
  • (Ribosomal RNA-processing protein ...) x 2
  • 13 kDa ribonucleoprotein-associated protein
  • (U3 small nucleolar RNA-associated protein ...) x 17
  • NET1-associated nuclear protein 1
  • Periodic tryptophan protein 2
  • Bud site selection protein 21
  • (U3 small nucleolar ribonucleoprotein protein ...) x 2
  • Something about silencing protein 10
  • Protein SOF1
  • (rRNA-processing protein ...) x 2
  • rRNA biogenesis protein RRP5
  • Ribosomal RNA small subunit methyltransferase NEP1
  • Ribosome biogenesis protein BMS1
  • RNA 3'-terminal phosphate cyclase-like protein
  • (Nucleolar complex protein ...) x 2
  • Essential nuclear protein 1
  • Pno1
  • Regulator of rDNA transcription protein 14
  • (Unassigned helices ...) x 2
  • (Exosome complex component ...) x 9
  • (Exosome complex exonuclease ...) x 2
  • Exosome complex protein LRP1
  • ATP-dependent RNA helicase DOB1
  • M-phase phosphoprotein 6 homolog
  • (ligand) x 3
Function / homology
Function and homology information


regulation of exoribonuclease activity / nuclear mRNA surveillance of spliceosomal pre-mRNA splicing / ncRNA polyadenylation / ncRNA 3'-end processing / TRAMP complex / nuclear mRNA surveillance of mRNA 3'-end processing / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process ...regulation of exoribonuclease activity / nuclear mRNA surveillance of spliceosomal pre-mRNA splicing / ncRNA polyadenylation / ncRNA 3'-end processing / TRAMP complex / nuclear mRNA surveillance of mRNA 3'-end processing / nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / 3'-5' RNA helicase activity / Noc4p-Nop14p complex / nuclear retention of pre-mRNA with aberrant 3'-ends at the site of transcription / nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' / CURI complex / t-UTP complex / nuclear polyadenylation-dependent CUT catabolic process / U1 snRNA 3'-end processing / nuclear retention of unspliced pre-mRNA at the site of transcription / UTP-C complex / cytoplasmic exosome (RNase complex) / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear retention of pre-mRNA at the site of transcription / nuclear polyadenylation-dependent mRNA catabolic process / sno(s)RNA processing / Pwp2p-containing subcomplex of 90S preribosome / U5 snRNA 3'-end processing / RNA fragment catabolic process / nuclear polyadenylation-dependent tRNA catabolic process / rRNA small subunit pseudouridine methyltransferase Nep1 / histone-glutamine methyltransferase activity / nuclear polyadenylation-dependent rRNA catabolic process / box C/D RNA binding / box C/D RNA 3'-end processing / CUT catabolic process / exosome (RNase complex) / U4 snRNA 3'-end processing / nuclear exosome (RNase complex) / Mpp10 complex / box H/ACA snoRNA binding / rRNA methyltransferase activity / polyadenylation-dependent snoRNA 3'-end processing / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA (pseudouridine) methyltransferase activity / septum digestion after cytokinesis / rRNA 2'-O-methylation / mRNA 3'-UTR AU-rich region binding => GO:0035925 / rRNA modification / nuclear mRNA surveillance / posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear-transcribed mRNA catabolic process, non-stop decay / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of transcription by RNA polymerase I / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of RNA binding / nuclear microtubule / rDNA heterochromatin / histone mRNA catabolic process / 90S preribosome assembly / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex / rRNA primary transcript binding / snRNA binding / rRNA metabolic process / nonfunctional rRNA decay / rRNA catabolic process / exonucleolytic catabolism of deadenylated mRNA / positive regulation of transcription by RNA polymerase I / rRNA base methylation / single-stranded telomeric DNA binding / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / maturation of 5.8S rRNA / protein localization to nucleolus / U4 snRNA binding / histone glutamine methylation / U3 snoRNA binding / poly(A) binding / Cul4-RING E3 ubiquitin ligase complex / RNA catabolic process / poly(A)+ mRNA export from nucleus / rRNA export from nucleus / sno(s)RNA-containing ribonucleoprotein complex / tRNA export from nucleus / precatalytic spliceosome / 90S preribosome / preribosome, small subunit precursor / O-methyltransferase activity / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / rRNA methylation / snoRNA binding / mRNA catabolic process / poly(U) RNA binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / regulation of translational fidelity / U4/U6 x U5 tri-snRNP complex / small-subunit processome / ribosomal small subunit biogenesis / nuclear periphery / maturation of LSU-rRNA
Nucleolar protein 14 / Brix domain / Bystin / Small-subunit processome, Utp21 / Sof1-like protein / Small-subunit processome, Utp12 / Sas10/Utp3/C1D / Small-subunit processome, Utp11 / Ribosome biogenesis protein BMS1/TSR1, C-terminal / HRDC-like superfamily ...Nucleolar protein 14 / Brix domain / Bystin / Small-subunit processome, Utp21 / Sof1-like protein / Small-subunit processome, Utp12 / Sas10/Utp3/C1D / Small-subunit processome, Utp11 / Ribosome biogenesis protein BMS1/TSR1, C-terminal / HRDC-like superfamily / rRNA-processing protein Fcf1/Utp23 / Small-subunit processome, Utp14 / Ribosomal protein S12/S23 / KOW / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4/S9, eukaryotic/archaeal / S15/NS1, RNA-binding / Quinoprotein alcohol dehydrogenase-like superfamily / CCAAT-binding factor / Nucleic acid-binding, OB-fold / BING4, C-terminal domain / AARP2CN / Ribosomal protein L23/L15e core domain superfamily / Nucleotide-binding alpha-beta plait domain superfamily / Ribosomal protein S13/S15, N-terminal / Exosome-associated factor Rrp6, N-terminal / Ribonuclease H-like superfamily / Exosome-associated factor Rrp47/DNA strand repair C1D / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / Tetratricopeptide-like helical domain superfamily / DEAD/DEAH box helicase domain / Down-regulated-in-metastasis protein / Zinc-binding ribosomal protein / Prismane-like superfamily / Ribosomal protein S23, eukaryotic/archaeal / NOL6/Upt22 / ATP-dependent RNA helicase Ski2, C-terminal / Fibrillarin / Ribosomal protein S3Ae / Ribosomal protein S6e / Exoribonuclease, phosphorolytic domain 1 / Ribosomal protein S8e / Ribosomal protein S4e / Ribosomal protein S9 / Ribosomal protein S8 / WD40 repeat / Ribosomal protein S27e / Ribosomal protein S15 / Ribosomal protein S7e / Ribosomal protein S28e / Ribosomal protein S17/S11 / Ribosomal protein S5/S7 / Helicase, C-terminal / Ribonuclease II/R / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / RNA-binding S4 domain / K Homology domain, type 1 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae conserved site / ATPase, AAA-type, core / HAT (Half-A-TPR) repeat / S1 domain / PIN domain / Ribosomal protein S4/S9, N-terminal / Nop domain / 3'-5' exonuclease domain / H/ACA ribonucleoprotein complex, subunit Nhp2, eukaryote / HRDC domain / Ribosomal protein S24e / Ribosomal protein S11 / BP28, C-terminal domain / NOP5, N-terminal / 40S ribosomal protein S11, N-terminal / Ribonuclease II/R, conserved site / Anaphase-promoting complex subunit 4, WD40 domain / Ribosomal RNA-processing protein 7, C-terminal domain / Ribosomal protein S7 domain / RNA 3'-terminal phosphate cyclase domain / Ribosomal protein S15P / RNA-binding domain, S1 / Ribosomal protein S4/S9 / Exosome complex component, N-terminal domain / Ribosomal protein S8e/ribosomal biogenesis NSA2 / U3 small nucleolar RNA-associated protein 10, N-terminal / HEAT, type 2 / Fibrillarin, conserved site / RNA 3'-terminal phosphate cyclase-like, conserved site / Ribosomal protein S7, conserved site / rRNA-processing arch domain / Exosome complex RNA-binding protein 1/RRP40/RRP4 / Ribosomal protein S5 domain 2-type fold / tRNA (guanine-N1-)-methyltransferase, N-terminal / Regulator of rDNA transcription 14 / Bms1/Tsr1-type G domain / 50S ribosomal protein L30e-like / S-adenosyl-L-methionine-dependent methyltransferase / PIN-like domain superfamily / Alpha/beta knot methyltransferases / Helix hairpin bin domain superfamily / Periodic tryptophan protein 2
U3 small nucleolar RNA-associated protein 8 / M-phase phosphoprotein 6 homolog / Exosome complex component CSL4 / U3 small nucleolar ribonucleoprotein protein IMP4 / U3 small nucleolar RNA-associated protein 6 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 14 / rRNA biogenesis protein RRP5 / rRNA-processing protein FCF1 ...U3 small nucleolar RNA-associated protein 8 / M-phase phosphoprotein 6 homolog / Exosome complex component CSL4 / U3 small nucleolar ribonucleoprotein protein IMP4 / U3 small nucleolar RNA-associated protein 6 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 14 / rRNA biogenesis protein RRP5 / rRNA-processing protein FCF1 / Exosome complex component RRP45 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / U3 small nucleolar RNA-associated protein 15 / Nucleolar complex protein 4 / Ribosomal RNA-processing protein 9 / U3 small nucleolar RNA-associated protein 12 / U3 small nucleolar RNA-associated protein 22 / 40S ribosomal protein S22-B / 40S ribosomal protein S28-A / Nucleolar protein 58 / Nucleolar protein 56 / Exosome complex component RRP42 / Exosome complex exonuclease RRP6 / U3 small nucleolar RNA-associated protein 4 / Something about silencing protein 10 / rRNA-processing protein FCF2 / Ribosome biogenesis protein BMS1 / Bud site selection protein 21 / Exosome complex component RRP40 / Exosome complex exonuclease DIS3 / RNA 3'-terminal phosphate cyclase-like protein / Exosome complex component RRP46 / 40S ribosomal protein S1-A / Exosome complex component MTR3 / 40S ribosomal protein S8-A / Periodic tryptophan protein 2 / Ribosomal RNA-processing protein 7 / Exosome complex component RRP43 / rRNA 2'-O-methyltransferase fibrillarin / 40S ribosomal protein S16-A / 40S ribosomal protein S11-A / 40S ribosomal protein S6-A / 40S ribosomal protein S7-A / 40S ribosomal protein S4-A / 40S ribosomal protein S24-A / 40S ribosomal protein S23-A / 40S ribosomal protein S14-A / 40S ribosomal protein S13 / 40S ribosomal protein S9-A / 40S ribosomal protein S5 / U3 small nucleolar ribonucleoprotein protein IMP3 / U3 small nucleolar RNA-associated protein MPP10 / 13 kDa ribonucleoprotein-associated protein / ATP-dependent RNA helicase DOB1 / Exosome complex component SKI6 / U3 small nucleolar RNA-associated protein 10 / Regulator of rDNA transcription protein 14 / U3 small nucleolar RNA-associated protein 18 / U3 small nucleolar RNA-associated protein 7 / U3 small nucleolar RNA-associated protein 9 / Protein SOF1 / Exosome complex protein LRP1 / Exosome complex component RRP4 / Essential nuclear protein 1 / 40S ribosomal protein S27-A / U3 small nucleolar RNA-associated protein 20 / U3 small nucleolar RNA-associated protein 11 / Nucleolar complex protein 14
Biological speciesSaccharomyces cerevisiae S288C (yeast) / Baker's yeast (baker's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsDu Y / Ye K
Funding support China, 2 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)91940302, 91540201, 31430024, 31325007 China
Chinese Academy of SciencesXDB08010203 China
CitationJournal: Science / Year: 2020
Title: Cryo-EM structure of 90 small ribosomal subunit precursors in transition states.
Authors: Yifei Du / Weidong An / Xing Zhu / Qi Sun / Jia Qi / Keqiong Ye /
Abstract: The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by ...The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by determining cryo-electron microscopy structures of intermediates in the path from the 90 to the pre-40 The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5' external transcribed spacer (5' ETS) is degraded within 90, driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90 through helicase Mtr4 and is primed to digest the 3' end of the 5' ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5' ETS degradation in 90 progression.
Validation ReportSummary, Full report, XML, About validation report
History
DepositionJan 14, 2020-
Header (metadata) releaseSep 16, 2020-
Map releaseSep 16, 2020-
UpdateSep 30, 2020-
Current statusSep 30, 2020Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.02
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  • Surface view colored by radius
  • Surface level: 0.02
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  • Surface view with fitted model
  • Atomic models: PDB-6lqs
  • Surface level: 0.02
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_0952.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.33 Å/pix.
x 448 pix.
= 597.632 Å
1.33 Å/pix.
x 448 pix.
= 597.632 Å
1.33 Å/pix.
x 448 pix.
= 597.632 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.334 Å
Density
Contour LevelBy AUTHOR: 0.02 / Movie #1: 0.02
Minimum - Maximum-0.0888008 - 0.14618781
Average (Standard dev.)0.00047531986 (±0.0038771639)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions448448448
Spacing448448448
CellA=B=C: 597.632 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.3341.3341.334
M x/y/z448448448
origin x/y/z0.0000.0000.000
length x/y/z597.632597.632597.632
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS448448448
D min/max/mean-0.0890.1460.000

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Supplemental data

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Additional map: Rrp5 map

Fileemd_0952_additional_1.map
AnnotationRrp5 map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Additional map: Exosome-90S map

Fileemd_0952_additional_2.map
AnnotationExosome-90S map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire 90S pre-ribosome (Dhr1-depleted, state D)

EntireName: 90S pre-ribosome (Dhr1-depleted, state D) / Number of components: 80

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Component #1: protein, 90S pre-ribosome (Dhr1-depleted, state D)

ProteinName: 90S pre-ribosome (Dhr1-depleted, state D) / Recombinant expression: No
MassTheoretical: 5 MDa
SourceSpecies: Saccharomyces cerevisiae S288C (yeast)

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Component #2: nucleic-acid, U3 snoRNA

nucleic acidName: U3 snoRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: GUCGACGUAC UUCAUAGGAU CAUUUCUAUA GGAAUCGUCA CUCUUUGACU CUUCAAAAGA GCCACUGAAU CCAACUUGGU UGAUGAGUCC CAUAACCUUU GUACCCCAGA GUGAGAAACC GAAAUUGAAU CUAAAUUAGC UUGGUCCGCA AUCCUUAGCG GUUCGGCCAU ...Sequence:
GUCGACGUAC UUCAUAGGAU CAUUUCUAUA GGAAUCGUCA CUCUUUGACU CUUCAAAAGA GCCACUGAAU CCAACUUGGU UGAUGAGUCC CAUAACCUUU GUACCCCAGA GUGAGAAACC GAAAUUGAAU CUAAAUUAGC UUGGUCCGCA AUCCUUAGCG GUUCGGCCAU CUAUAAUUUU GAAUAAAAAU UUUGCUUUGC CGUUGCAUUU GUAGUUUUUU CCUUUGGAAG UAAUUACAAU AUUUUAUGGC GCGAUGAUCU UGACCCAUCC UAUGUACUUC UUUUUUGAAG GGAUAGGGCU CUAUGGGUGG GUACAAAUGG CAGUCUGACA AGU
MassTheoretical: 106.503258 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #3: nucleic-acid, 5' ETS

nucleic acidName: 5' ETS / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: AUGCGAAAGC AGUUGAAGAC AAGUUCGAAA AGAGUUUGGA AACGAAUUCG AGUAGGCUUG UCGUUCGUUA UGUUUUUGUA AAUGGCCUCG UCAAACGGUG GAGAGAGUCG CUAGGUGAUC GUCAGAUCUG CCUAGUCUCU AUACAGCGUG UUUAAUUGAC AUGGGUUGAU ...Sequence:
AUGCGAAAGC AGUUGAAGAC AAGUUCGAAA AGAGUUUGGA AACGAAUUCG AGUAGGCUUG UCGUUCGUUA UGUUUUUGUA AAUGGCCUCG UCAAACGGUG GAGAGAGUCG CUAGGUGAUC GUCAGAUCUG CCUAGUCUCU AUACAGCGUG UUUAAUUGAC AUGGGUUGAU GCGUAUUGAG AGAUACAAUU UGGGAAGAAA UUCCCAGAGU GUGUUUCUUU UGCGUUUAAC CUGAACAGUC UCAUCGUGGG CAUCUUGCGA UUCCAUUGGU GAGCAGCGAA GGAUUUGGUG GAUUACUAGC UAAUAGCAAU CUAUUUCAAA GAAUUCAAAC UUGGGGGAAU GCCUUGUUGA AUAGCCGGUC GCAAGACUGU GAUUCUUCAA GUGUAACCUC CUCUCAAAUC AGCGAUAUCA AACGUACCAU UCCGUGAAAC ACCGGGGUAU CUGUUUGGUG GAACCUGAUU AGAGGAAACU CAAAGAGUGC UAUGGUAUGG UGACGGAGUG CGCUGGUCAA GAGUGUAAAA GCUUUUUGAA CAGAGAGCAU UUCCGGCAGC AGAGAGACCU GAAAAAGCAA UUUUUCUGGA AUUUCAGCUG UUUCCAAACU CAAUAAGUAU CUUCUAGCAA GAGGGAAUAG GUGGGAAAAA AAAAAAGAGA UUUCGGUUUC UUUCUUUUUU ACUGCUUGUU GCUUCUUCUU UUAAGAUAGU
MassTheoretical: 225.543094 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #4: nucleic-acid, 18S pre-rRNA

nucleic acidName: 18S pre-rRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: AAGAUAGUUA UCUGGUUGAU CCUGCCAGUA GUCAUAUGCU UGUCUCAAAG AUUAAGCCAU GCAUGUCUAA GUAUAAGCAA UUUAUACAGU GAAACUGCGA AUGGCUCAUU AAAUCAGUUA UCGUUUAUUU GAUAGUUCCU UUACUACAUG GUAUAACUGU GGUAAUUCUA ...Sequence:
AAGAUAGUUA UCUGGUUGAU CCUGCCAGUA GUCAUAUGCU UGUCUCAAAG AUUAAGCCAU GCAUGUCUAA GUAUAAGCAA UUUAUACAGU GAAACUGCGA AUGGCUCAUU AAAUCAGUUA UCGUUUAUUU GAUAGUUCCU UUACUACAUG GUAUAACUGU GGUAAUUCUA GAGCUAAUAC AUGCUUAAAA UCUCGACCCU UUGGAAGAGA UGUAUUUAUU AGAUAAAAAA UCAAUGUCUU CGGACUCUUU GAUGAUUCAU AAUAACUUUU CGAAUCGCAU GGCCUUGUGC UGGCGAUGGU UCAUUCAAAU UUCUGCCCUA UCAACUUUCG AUGGUAGGAU AGUGGCCUAC CAUGGUUUCA ACGGGUAACG GGGAAUAAGG GUUCGAUUCC GGAGAGGGAG CCUGAGAAAC GGCUACCACA UCCAAGGAAG GCAGCAGGCG CGCAAAUUAC CCAAUCCUAA UUCAGGGAGG UAGUGACAAU AAAUAACGAU ACAGGGCCCA UUCGGGUCUU GUAAUUGGAA UGAGUACAAU GUAAAUACCU UAACGAGGAA CAAUUGGAGG GCAAGUCUGG UGCCAGCAGC CGCGGUAAUU CCAGCUCCAA UAGCGUAUAU UAAAGUUGUU GCAGUUAAAA AGCUCGUAGU UGAACUUUGG GCCCGGUUGG CCGGUCCGAU UUUUUCGUGU ACUGGAUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUGUG GCUCUUGGCG AACCAGGACU UUUACUUUGA AAAAAUUAGA GUGUUCAAAG CAGGCGUAUU GCUCGAAUAU AUUAGCAUGG AAUAAUAGAA UAGGACGUUU GGUUCUAUUU UGUUGGUUUC UAGGACCAUC GUAAUGAUUA AUAGGGACGG UCGGGGGCAU CAGUAUUCAA UUGUCAGAGG UGAAAUUCUU GGAUUUAUUG AAGACUAACU ACUGCGAAAG CAUUUGCCAA GGACGUUUUC AUUAAUCAAG AACGAAAGUU AGGGGAUCGA AGAUGAUCAG AUACCGUCGU AGUCUUAACC AUAAACUAUG CCGACUAGGG AUCGGGUGGU GUUUUUUUAA UGACCCACUC GGCACCUUAC GAGAAAUCAA AGUCUUUGGG UUCUGGGGGG AGUAUGGUCG CAAGGCUGAA ACUUAAAGGA AUUGACGGAA GGGCACCACC AGGAGUGGAG CCUGCGGCUU AAUUUGACUC AACACGGGGA AACUCACCAG GUCCAGACAC AAUAAGGAUU GACAGAUUGA GAGCUCUUUC UUGAUUUUGU GGGUGGUGGU GCAUGGCCGU UCUUAGUUGG UGGAGUGAUU UGUCUGCUUA AUUGCGAUAA CGAACGAGAC CUUAACCUAC UAAAUAGUGG UGCUAGCAUU UGCUGGUUAU CCACUUCUUA GAGGGACUAU CGGUUUCAAG CCGAUGGAAG UUUGAGGCAA UAACAGGUCU GUGAUGCCCU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUUGU GAAACUCCGU CGUGCUGGGG AUAGAGCAUU GUAAUUAUUG CUCUUCAACG AGGAAUUCCU AGUAAGCGCA AGUCAUCAGC UUGCGUUGAU UACGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUAGUAC CGAUUGAAUG GCUUAGUGAG GCCUCAGGAU CUGCUUAGAG AAGGGGGCAA CUCCAUCUCA GAGCGGAGAA UUUGGACAAA CUUGGUCAUU UAGAGGAACU AAAAGUCGUA ACAAGGUUUC CGUAGGUGAA CCUGCGGAAG GAUCAUUAA
MassTheoretical: 582.710875 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #5: protein, 40S ribosomal protein S1-A

ProteinName: 40S ribosomal protein S1-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.798467 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #6: protein, 40S ribosomal protein S4-A

ProteinName: 40S ribosomal protein S4-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.46933 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #7: protein, 40S ribosomal protein S5

ProteinName: 40S ribosomal protein S5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.0726 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #8: protein, 40S ribosomal protein S6-A

ProteinName: 40S ribosomal protein S6-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.054486 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #9: protein, 40S ribosomal protein S7-A

ProteinName: 40S ribosomal protein S7-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.658209 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #10: protein, 40S ribosomal protein S8-A

ProteinName: 40S ribosomal protein S8-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.537803 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #11: protein, 40S ribosomal protein S9-A

ProteinName: 40S ribosomal protein S9-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.487893 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #12: protein, 40S ribosomal protein S11-A

ProteinName: 40S ribosomal protein S11-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.785934 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #13: protein, 40S ribosomal protein S13

ProteinName: 40S ribosomal protein S13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.059945 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #14: protein, 40S ribosomal protein S14-A

ProteinName: 40S ribosomal protein S14-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.562655 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #15: protein, 40S ribosomal protein S16-A

ProteinName: 40S ribosomal protein S16-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.87749 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #16: protein, 40S ribosomal protein S22-B

ProteinName: 40S ribosomal protein S22-BRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.650062 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #17: protein, 40S ribosomal protein S23-A

ProteinName: 40S ribosomal protein S23-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.073896 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

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Component #18: protein, 40S ribosomal protein S24-A

ProteinName: 40S ribosomal protein S24-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.362848 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #19: protein, 40S ribosomal protein S27-A

ProteinName: 40S ribosomal protein S27-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.893391 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #20: protein, 40S ribosomal protein S28-A

ProteinName: 40S ribosomal protein S28-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.605847 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #21: protein, rRNA 2'-O-methyltransferase fibrillarin

ProteinName: rRNA 2'-O-methyltransferase fibrillarin / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 34.525418 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #22: protein, Nucleolar protein 56

ProteinName: Nucleolar protein 56Nucleolus / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 56.961152 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #23: protein, Nucleolar protein 58

ProteinName: Nucleolar protein 58Nucleolus / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 57.060344 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #24: protein, Ribosomal RNA-processing protein 9

ProteinName: Ribosomal RNA-processing protein 9 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 65.146969 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #25: protein, 13 kDa ribonucleoprotein-associated protein

ProteinName: 13 kDa ribonucleoprotein-associated protein / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 13.582855 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #26: protein, U3 small nucleolar RNA-associated protein 4

ProteinName: U3 small nucleolar RNA-associated protein 4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 87.909242 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #27: protein, U3 small nucleolar RNA-associated protein 5

ProteinName: U3 small nucleolar RNA-associated protein 5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 72.079445 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #28: protein, U3 small nucleolar RNA-associated protein 8

ProteinName: U3 small nucleolar RNA-associated protein 8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 80.269648 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #29: protein, U3 small nucleolar RNA-associated protein 9

ProteinName: U3 small nucleolar RNA-associated protein 9 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 65.347254 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #30: protein, U3 small nucleolar RNA-associated protein 10

ProteinName: U3 small nucleolar RNA-associated protein 10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 200.298984 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #31: protein, U3 small nucleolar RNA-associated protein 15

ProteinName: U3 small nucleolar RNA-associated protein 15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 57.765289 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #32: protein, NET1-associated nuclear protein 1

ProteinName: NET1-associated nuclear protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 101.341734 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #33: protein, Periodic tryptophan protein 2

ProteinName: Periodic tryptophan protein 2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 101.467367 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #34: protein, U3 small nucleolar RNA-associated protein 12

ProteinName: U3 small nucleolar RNA-associated protein 12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 106.481133 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #35: protein, U3 small nucleolar RNA-associated protein 13

ProteinName: U3 small nucleolar RNA-associated protein 13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 91.132562 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #36: protein, U3 small nucleolar RNA-associated protein 18

ProteinName: U3 small nucleolar RNA-associated protein 18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 66.49425 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #37: protein, U3 small nucleolar RNA-associated protein 21

ProteinName: U3 small nucleolar RNA-associated protein 21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 104.927844 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #38: protein, U3 small nucleolar RNA-associated protein 6

ProteinName: U3 small nucleolar RNA-associated protein 6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 52.495277 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #39: protein, Bud site selection protein 21

ProteinName: Bud site selection protein 21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.431016 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #40: protein, U3 small nucleolar RNA-associated protein 7

ProteinName: U3 small nucleolar RNA-associated protein 7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 62.41857 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #41: protein, U3 small nucleolar RNA-associated protein 11

ProteinName: U3 small nucleolar RNA-associated protein 11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.806348 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #42: protein, U3 small nucleolar RNA-associated protein MPP10

ProteinName: U3 small nucleolar RNA-associated protein MPP10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 67.042492 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #43: protein, U3 small nucleolar ribonucleoprotein protein IMP3

ProteinName: U3 small nucleolar ribonucleoprotein protein IMP3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.928529 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #44: protein, U3 small nucleolar ribonucleoprotein protein IMP4

ProteinName: U3 small nucleolar ribonucleoprotein protein IMP4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.536168 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #45: protein, Something about silencing protein 10

ProteinName: Something about silencing protein 10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 70.364398 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #46: protein, Protein SOF1

ProteinName: Protein SOF1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 56.888918 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #47: protein, rRNA-processing protein FCF2

ProteinName: rRNA-processing protein FCF2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.68924 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #48: protein, rRNA-processing protein FCF1

ProteinName: rRNA-processing protein FCF1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.650729 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #49: protein, rRNA biogenesis protein RRP5

ProteinName: rRNA biogenesis protein RRP5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 193.411422 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #50: protein, U3 small nucleolar RNA-associated protein 22

ProteinName: U3 small nucleolar RNA-associated protein 22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 140.660141 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #51: protein, Ribosomal RNA-processing protein 7

ProteinName: Ribosomal RNA-processing protein 7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.526441 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #52: protein, Ribosomal RNA small subunit methyltransferase NEP1

ProteinName: Ribosomal RNA small subunit methyltransferase NEP1 / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 27.936461 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #53: protein, Ribosome biogenesis protein BMS1

ProteinName: Ribosome biogenesis protein BMS1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 135.792281 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #54: protein, RNA 3'-terminal phosphate cyclase-like protein

ProteinName: RNA 3'-terminal phosphate cyclase-like protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 40.220559 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #55: protein, Nucleolar complex protein 14

ProteinName: Nucleolar complex protein 14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 94.463195 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #56: protein, Nucleolar complex protein 4

ProteinName: Nucleolar complex protein 4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 63.707844 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #57: protein, U3 small nucleolar RNA-associated protein 20

ProteinName: U3 small nucleolar RNA-associated protein 20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 287.9155 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #58: protein, U3 small nucleolar RNA-associated protein 14

ProteinName: U3 small nucleolar RNA-associated protein 14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 103.18975 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #59: protein, Essential nuclear protein 1

ProteinName: Essential nuclear protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 54.879105 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #60: protein, Pno1

ProteinName: Pno1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 36.356477 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #61: protein, Regulator of rDNA transcription protein 14

ProteinName: Regulator of rDNA transcription protein 14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.898289 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #62: protein, Unassigned helices 1

ProteinName: Unassigned helices 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.549291 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #63: protein, Unassigned helices 2

ProteinName: Unassigned helices 2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 59.080809 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #64: protein, Exosome complex component RRP45

ProteinName: Exosome complex component RRP45 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.001859 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #65: protein, Exosome complex component SKI6

ProteinName: Exosome complex component SKI6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.599727 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #66: protein, Exosome complex component RRP43

ProteinName: Exosome complex component RRP43 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 44.060934 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #67: protein, Exosome complex component RRP46

ProteinName: Exosome complex component RRP46 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.430193 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #68: protein, Exosome complex component RRP42

ProteinName: Exosome complex component RRP42 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.085174 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #69: protein, Exosome complex component MTR3

ProteinName: Exosome complex component MTR3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.603988 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #70: protein, Exosome complex component RRP40

ProteinName: Exosome complex component RRP40 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.583354 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #71: protein, Exosome complex component RRP4

ProteinName: Exosome complex component RRP4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 39.470176 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #72: protein, Exosome complex component CSL4

ProteinName: Exosome complex component CSL4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 31.619279 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #73: protein, Exosome complex exonuclease DIS3

ProteinName: Exosome complex exonuclease DIS3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 113.857734 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #74: protein, Exosome complex exonuclease RRP6

ProteinName: Exosome complex exonuclease RRP6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 84.16057 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #75: protein, Exosome complex protein LRP1

ProteinName: Exosome complex protein LRP1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.086297 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #76: protein, ATP-dependent RNA helicase DOB1

ProteinName: ATP-dependent RNA helicase DOB1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 122.228031 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #77: protein, M-phase phosphoprotein 6 homolog

ProteinName: M-phase phosphoprotein 6 homolog / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.185549 kDa
SourceSpecies: Baker's yeast (baker's yeast) / Strain: ATCC 204508 / S288c

+
Component #78: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

+
Component #79: ligand, GUANOSINE-5'-TRIPHOSPHATE

LigandName: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.52318 kDa

+
Component #80: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

-
Experimental details

-
Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionSpecimen conc.: 2 mg/mL / pH: 7.4
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 %

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 50 e/Å2 / Illumination mode: FLOOD BEAM
LensCs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: 1500.0 - 2500.0 nm
Specimen HolderModel: FEI TITAN KRIOS AUTOGRID HOLDER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

-
Image acquisition

Image acquisitionNumber of digital images: 18028

-
Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 75404
3D reconstructionSoftware: RELION / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

-
Atomic model buiding

Modeling #1Input PDB model: 6LQP
Output model

+
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