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TitleCryo-EM structure of 90 small ribosomal subunit precursors in transition states.
Journal, issue, pagesScience, Vol. 369, Issue 6510, Page 1477-1481, Year 2020
Publish dateSep 18, 2020
AuthorsYifei Du / Weidong An / Xing Zhu / Qi Sun / Jia Qi / Keqiong Ye /
PubMed AbstractThe 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by ...The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by determining cryo-electron microscopy structures of intermediates in the path from the 90 to the pre-40 The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5' external transcribed spacer (5' ETS) is degraded within 90, driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90 through helicase Mtr4 and is primed to digest the 3' end of the 5' ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5' ETS degradation in 90 progression.
External linksScience / PubMed:32943522
MethodsEM (single particle)
Resolution3.2 - 8.6 Å
Structure data

EMDB-0949, PDB-6lqp:
Cryo-EM structure of 90S small subunit preribosomes in transition states (State A)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-0950, PDB-6lqq:
Cryo-EM structure of 90S small subunit preribosomes in transition states (State B)
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-0951, PDB-6lqr:
Cryo-EM structure of 90S small subunit preribosomes in transition states (State C)
Method: EM (single particle) / Resolution: 8.6 Å

EMDB-0952, PDB-6lqs:
Cryo-EM structure of 90S small subunit preribosomes in transition states (State D)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-0953, PDB-6lqt:
Cryo-EM structure of 90S small subunit preribosomes in transition states (State E)
Method: EM (single particle) / Resolution: 4.9 Å

EMDB-0954, PDB-6lqu:
Cryo-EM structure of 90S small subunit preribosomes in transition states (State A1)
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-0955, PDB-6lqv:
Cryo-EM structure of 90S small subunit preribosomes in transition states (State C1)
Method: EM (single particle) / Resolution: 4.8 Å

Chemicals

ChemComp-ZN:
ZINC ION / Zinc

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
MAGNESIUM ION / Magnesium

Source
  • saccharomyces cerevisiae s288c (yeast)
  • Baker's yeast (baker's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsCryoelectron Microscopy / DEAD-box RNA Helicases / ECM16 protein, S cerevisiae / Exosomes / Gene Deletion / MTR4 protein, S cerevisiae / Protein Domains / RNA Stability / RNA, Ribosomal, 18S / Ribosome Subunits, Large, Eukaryotic / Ribosome Subunits, Small, Eukaryotic / Saccharomyces cerevisiae / Saccharomyces cerevisiae Proteins / RIBOSOME / ribosome assembly / 90S to pre-40S transition / cryo-EM / Dhr1

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