rRNA acetylation involved in maturation of SSU-rRNA / Noc4p-Nop14p complex / rRNA cytidine N-acetyltransferase activity / t-UTP complex / tRNA acetylation / Pwp2p-containing subcomplex of 90S preribosome / sno(s)RNA processing / RNA fragment catabolic process / rRNA small subunit pseudouridine methyltransferase Nep1 / mRNA modification ...rRNA acetylation involved in maturation of SSU-rRNA / Noc4p-Nop14p complex / rRNA cytidine N-acetyltransferase activity / t-UTP complex / tRNA acetylation / Pwp2p-containing subcomplex of 90S preribosome / sno(s)RNA processing / RNA fragment catabolic process / rRNA small subunit pseudouridine methyltransferase Nep1 / mRNA modification / histone-glutamine methyltransferase activity / box C/D RNA 3'-end processing / Mpp10 complex / rRNA methyltransferase activity / rRNA (pseudouridine) methyltransferase activity / rRNA 2'-O-methylation / septum digestion after cytokinesis / rRNA modification / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of transcription by RNA polymerase I / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of RNA binding / nuclear microtubule / rDNA heterochromatin / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex / snRNA binding / rRNA primary transcript binding / positive regulation of transcription by RNA polymerase I / rRNA base methylation / single-stranded telomeric DNA binding / protein localization to nucleolus / small nuclear ribonucleoprotein complex / U4 snRNA binding / histone glutamine methylation / sno(s)RNA-containing ribonucleoprotein complex / U3 snoRNA binding / Cul4-RING E3 ubiquitin ligase complex / poly(A)+ mRNA export from nucleus / rRNA export from nucleus / tRNA export from nucleus / precatalytic spliceosome / 90S preribosome / preribosome, small subunit precursor / O-methyltransferase activity / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / rRNA methylation / snoRNA binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / U4/U6 x U5 tri-snRNP complex / regulation of translational fidelity / small-subunit processome / ribosomal small subunit biogenesis / nuclear periphery / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / maturation of LSU-rRNA / Cajal body / establishment of cell polarity / endoribonuclease activity / vesicle-mediated transport / maintenance of translational fidelity / positive regulation of translational fidelity / enzyme activator activity / ribosomal small subunit assembly / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA processing / mRNA splicing, via spliceosome / cytoplasmic translation / Transferases; Transferring one-carbon groups; Methyltransferases / protein transport / cytosolic small ribosomal subunit / small ribosomal subunit / tRNA binding / ribosome / rRNA binding / structural constituent of ribosome / cell cycle / translation / ATPase / GTPase activity / mRNA binding / GTP binding / nucleolus / mitochondrion / RNA binding / nucleoplasm / ATP binding / identical protein binding / nucleus / cytosol / cytoplasm
Nucleic acid-binding, OB-fold / NUC153 / Nucleolar protein 14 / Sof1-like protein / Small-subunit processome, Utp21 / Helicase domain / Bystin / Quinoprotein alcohol dehydrogenase-like superfamily / Down-regulated-in-metastasis protein / Tetratricopeptide-like helical domain superfamily ...Nucleic acid-binding, OB-fold / NUC153 / Nucleolar protein 14 / Sof1-like protein / Small-subunit processome, Utp21 / Helicase domain / Bystin / Quinoprotein alcohol dehydrogenase-like superfamily / Down-regulated-in-metastasis protein / Tetratricopeptide-like helical domain superfamily / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / Apoptosis-antagonizing transcription factor, C-terminal / Sas10/Utp3/C1D / Ribosomal protein L23/L15e core domain superfamily / AARP2CN / BING4, C-terminal domain / BP28, C-terminal domain / NOP5, N-terminal / NOSIC / U3 snoRNA associated / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / RNA 3'-terminal phosphate cyclase, insert domain / Small-subunit processome, Utp12 / Small-subunit processome, Utp11 / Ribosomal protein S4e, N-terminal / Ribosomal protein L7Ae conserved site / WD40 repeat / Ribosomal protein S4/S9, N-terminal / Ribosomal protein S24e / H/ACA ribonucleoprotein complex, subunit Nhp2, eukaryote / Nop domain / PIN domain / RNA-binding S4 domain / HAT (Half-A-TPR) repeat / ATPase, AAA-type, core / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Brix domain / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / CCAAT-binding factor / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S5/S7, eukaryotic/archaeal / KOW / Ribosomal protein S12/S23 / Small-subunit processome, Utp14 / rRNA-processing protein Fcf1/Utp23 / Ribosome biogenesis protein BMS1/TSR1, C-terminal / RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta / Ribosomal protein S4e, central region / Ribosomal protein S8e / Periodic tryptophan protein 2 / Fibrillarin, conserved site / HEAT, type 2 / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S4/S9 / RNA 3'-terminal phosphate cyclase domain / Ribosomal protein S7 domain / Anaphase-promoting complex subunit 4, WD40 domain / AATF leucine zipper-containing domain / Nucleolar complex protein 4 / Ribosomal protein S7, conserved site / P-loop containing nucleoside triphosphate hydrolase / Possible tRNA binding domain / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S28e conserved site / Helix hairpin bin domain superfamily / tRNA (guanine-N1-)-methyltransferase, N-terminal / Alpha/beta knot methyltransferases / PIN-like domain superfamily / S-adenosyl-L-methionine-dependent methyltransferase / 50S ribosomal protein L30e-like / RNA 3'-terminal phosphate cyclase-like, conserved site / Ribosomal protein S9, conserved site / Small-subunit processome, Utp13 / Ribosomal protein S4, conserved site / U3 small nucleolar RNA-associated protein 6 / Ribosomal protein S6, eukaryotic / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein L2, domain 2 / Fcf2 pre-rRNA processing, C-terminal / WD40/YVTN repeat-like-containing domain superfamily / Armadillo-type fold / RNA 3'-terminal phosphate cyclase type 2 / WD40-repeat-containing domain / Ribosomal S24e conserved site / Ribosomal protein S5 domain 2-type fold / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S6e, conserved site / Ribosomal protein S8e, conserved site / Ribosomal protein L7Ae/L8/Nhp2 family / U3 small nucleolar RNA-associated protein 8 / Sas10 C-terminal domain / U3 small nucleolar RNA-associated protein 15, C-terminal / WD40 repeat, conserved site / Ribosomal protein S17, conserved site / G-protein beta WD-40 repeat / Ribosomal protein S6e / Ribosomal protein S4e / 40S ribosomal protein S4, C-terminal domain
Ribosomal RNA-processing protein 9 / U3 small nucleolar RNA-associated protein 6 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 15 / U3 small nucleolar RNA-associated protein 14 / rRNA-processing protein FCF1 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 ...Ribosomal RNA-processing protein 9 / U3 small nucleolar RNA-associated protein 6 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 15 / U3 small nucleolar RNA-associated protein 14 / rRNA-processing protein FCF1 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / Nucleolar protein 58 / Nucleolar complex protein 4 / Protein BFR2 / U3 small nucleolar RNA-associated protein 4 / RNA 3'-terminal phosphate cyclase-like protein / Bud site selection protein 21 / Ribosome biogenesis protein BMS1 / rRNA-processing protein FCF2 / Something about silencing protein 10 / U3 small nucleolar RNA-associated protein 12 / Nucleolar protein 56 / RNA cytidine acetyltransferase / 40S ribosomal protein S28-A / U3 small nucleolar ribonucleoprotein protein IMP4 / U3 small nucleolar RNA-associated protein 20 / U3 small nucleolar RNA-associated protein 8 / 40S ribosomal protein S5 / 40S ribosomal protein S9-A / 40S ribosomal protein S23-A / 40S ribosomal protein S24-A / 40S ribosomal protein S4-A / 40S ribosomal protein S6-A / 40S ribosomal protein S8-A / 40S ribosomal protein S11-A / 40S ribosomal protein S16-A / rRNA 2'-O-methyltransferase fibrillarin / Periodic tryptophan protein 2 / U3 small nucleolar ribonucleoprotein protein IMP3 / Ribosome biogenesis protein ENP2 / Protein SOF1 / U3 small nucleolar RNA-associated protein 11 / Essential nuclear protein 1 / U3 small nucleolar RNA-associated protein 9 / 13 kDa ribonucleoprotein-associated protein / U3 small nucleolar RNA-associated protein 7 / U3 small nucleolar ribonucleoprotein protein LCP5 / U3 small nucleolar RNA-associated protein 18 / U3 small nucleolar RNA-associated protein 10 / U3 small nucleolar RNA-associated protein MPP10 / Nucleolar complex protein 14
National Natural Science Foundation of China (NSFC)
91940302, 91540201, 31430024, 31325007
China
Chinese Academy of Sciences
XDB08010203
China
Citation
Journal: Science / Year: 2020 Title: Cryo-EM structure of 90 small ribosomal subunit precursors in transition states. Authors: Yifei Du / Weidong An / Xing Zhu / Qi Sun / Jia Qi / Keqiong Ye / Abstract: The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by ...The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by determining cryo-electron microscopy structures of intermediates in the path from the 90 to the pre-40 The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5' external transcribed spacer (5' ETS) is degraded within 90, driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90 through helicase Mtr4 and is primed to digest the 3' end of the 5' ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5' ETS degradation in 90 progression.
3A: U3 snoRNA 5A: 5' ETS SA: 18S pre-rRNA SF: 40S ribosomal protein S4-A SG: 40S ribosomal protein S5 SH: 40S ribosomal protein S6-A SJ: 40S ribosomal protein S8-A SK: 40S ribosomal protein S9-A SM: 40S ribosomal protein S11-A SR: 40S ribosomal protein S16-A SY: 40S ribosomal protein S23-A SZ: 40S ribosomal protein S24-A Sd: 40S ribosomal protein S28-A 3B: rRNA 2'-O-methyltransferase fibrillarin 3C: rRNA 2'-O-methyltransferase fibrillarin 3D: Nucleolar protein 56 3E: Nucleolar protein 58 3F: Ribosomal RNA-processing protein 9 3G: 13 kDa ribonucleoprotein-associated protein 3H: 13 kDa ribonucleoprotein-associated protein A4: U3 small nucleolar RNA-associated protein 4 A5: U3 small nucleolar RNA-associated protein 5 A8: U3 small nucleolar RNA-associated protein 8 A9: U3 small nucleolar RNA-associated protein 9 AE: U3 small nucleolar RNA-associated protein 10 AF: U3 small nucleolar RNA-associated protein 15 AG: NET1-associated nuclear protein 1 B1: Periodic tryptophan protein 2 B2: U3 small nucleolar RNA-associated protein 12 B3: U3 small nucleolar RNA-associated protein 13 B8: U3 small nucleolar RNA-associated protein 18 BE: U3 small nucleolar RNA-associated protein 21 B6: U3 small nucleolar RNA-associated protein 6 5B: Bud site selection protein 21 5C: U3 small nucleolar RNA-associated protein 7 5D: U3 small nucleolar RNA-associated protein 11 5E: U3 small nucleolar RNA-associated protein MPP10 5F: U3 small nucleolar ribonucleoprotein protein IMP3 5G: U3 small nucleolar ribonucleoprotein protein IMP4 5H: Something about silencing protein 10 5I: Protein SOF1 5J: rRNA-processing protein FCF2 5K: rRNA-processing protein FCF1 RA: Ribosome biogenesis protein ENP2 RB: U3 small nucleolar ribonucleoprotein protein LCP5 RG: Ribosomal RNA small subunit methyltransferase NEP1 RH: Ribosomal RNA small subunit methyltransferase NEP1 RJ: Ribosome biogenesis protein BMS1 RK: RNA 3'-terminal phosphate cyclase-like protein RL: RNA cytidine acetyltransferase RM: RNA cytidine acetyltransferase RN: Nucleolar complex protein 14 RO: Nucleolar complex protein 4 RP: U3 small nucleolar RNA-associated protein 20 RQ: U3 small nucleolar RNA-associated protein 14 RS: Essential nuclear protein 1 RY: Protein BFR2 X1: Unassigned helices hetero molecules
Mass: 7605.847 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q3E7X9
+
Protein , 14 types, 18 molecules 3B3C3F3G3HAGB15B5H5IRGRHRKRLRMRSRYX1
#14: Protein
rRNA2'-O-methyltransferasefibrillarin / Nop1 / Histone-glutamine methyltransferase / U3 small nucleolar RNA-associated protein NOP1 / U3 ...Nop1 / Histone-glutamine methyltransferase / U3 small nucleolar RNA-associated protein NOP1 / U3 snoRNA-associated protein NOP1
Mass: 34525.418 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c References: UniProt: P15646, Transferases; Transferring one-carbon groups; Methyltransferases
#17: Protein
RibosomalRNA-processingprotein9 / Rrp9
Mass: 65146.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q06506
#18: Protein
13kDaribonucleoprotein-associatedprotein / Snu13 / Small nuclear ribonucleoprotein-associated protein 1
Mass: 13582.855 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P39990
#25: Protein
NET1-associatednuclearprotein1 / Utp17 / U three protein 17 / t-17 / U3 protein 17 required for transcription / U3 small nucleolar ...Utp17 / U three protein 17 / t-17 / U3 protein 17 required for transcription / U3 small nucleolar RNA-associated protein 17 / U3 snoRNA-associated protein 17
Mass: 101341.734 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q02931
#26: Protein
Periodictryptophanprotein2 / Utp1 / U three protein 1 / U3 small nucleolar RNA-associated protein 1 / U3 snoRNA-associated protein 1
Mass: 104097.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P25635
#32: Protein
Budsiteselectionprotein21 / Bud21 / U three protein 16 / U3 small nucleolar RNA-associated protein 16 / U3 snoRNA-associated protein 16
Mass: 24431.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q08492
#38: Protein
Somethingaboutsilencingprotein10 / Sas10 / U three protein 3 / U3 small nucleolar RNA-associated protein 3 / U3 snoRNA-associated protein 11
Mass: 70364.398 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q12136
#39: Protein
ProteinSOF1 / Sof1 / U3 small nucleolar RNA-associated protein SOF1 / U3 snoRNA-associated protein SOF1
Mass: 56888.918 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P33750
Mass: 119509.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c References: UniProt: P53914, Transferases; Acyltransferases; Transferring groups other than aminoacyl groups
#52: Protein
Essentialnuclearprotein1 / Enp1
Mass: 55207.422 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P38333
#53: Protein
ProteinBFR2 / Brefeldin A resistance protein 2
Mass: 61285.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q06631
#54: Protein
Unassignedhelices
Mass: 29549.291 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
+
Nucleolar protein ... , 2 types, 2 molecules 3D3E
#15: Protein
Nucleolarprotein56 / Nucleolus / Nop56 / Ribosome biosynthesis protein SIK1 / Suppressor of I kappa b protein 1
Mass: 56961.152 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q12460
#16: Protein
Nucleolarprotein58 / Nucleolus / Nucleolar protein 5
Mass: 57060.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q12499
+
U3 small nucleolar RNA-associated protein ... , 16 types, 16 molecules A4A5A8A9AEAFB2B3B8BEB65C5D5ERPRQ
#19: Protein
U3smallnucleolarRNA-associatedprotein4 / Utp4 / U3 snoRNA-associated protein 4 / U three protein 4 / U3 protein 4 required for transcription / t-UTP4
Mass: 87909.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q06679
#20: Protein
U3smallnucleolarRNA-associatedprotein5 / Utp5 / U3 snoRNA-associated protein 5 / U three protein 5 / U3 protein 5 required for transcription / t-UTP5
Mass: 72079.445 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q04177
#21: Protein
U3smallnucleolarRNA-associatedprotein8 / Utp8 / U3 snoRNA-associated protein 8 / U three protein 8 / U3 protein 8 required for transcription / t-UTP8
Mass: 80269.648 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P53276
#22: Protein
U3smallnucleolarRNA-associatedprotein9 / Utp9 / U3 snoRNA-associated protein 9 / U three protein 9 / U3 protein 9 required for transcription / t-UTP9
Mass: 65347.254 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P38882
#23: Protein
U3smallnucleolarRNA-associatedprotein10 / Utp10 / U3 snoRNA-associated protein 10 / U three protein 10 / U3 protein 10 required for ...Utp10 / U3 snoRNA-associated protein 10 / U three protein 10 / U3 protein 10 required for transcription / t-UTP10
Mass: 200298.984 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P42945
#24: Protein
U3smallnucleolarRNA-associatedprotein15 / Utp15 / U3 snoRNA-associated protein 15 / U three protein 15 / U3 protein 15 required for ...Utp15 / U3 snoRNA-associated protein 15 / U three protein 15 / U3 protein 15 required for transcription / t-UTP15
Mass: 57765.289 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q04305
#27: Protein
U3smallnucleolarRNA-associatedprotein12 / Utp12 / U3 snoRNA-associated protein 12 / DOM34-interacting protein 2 / U three protein 12
Mass: 106481.133 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q12220
#28: Protein
U3smallnucleolarRNA-associatedprotein13 / Utp13 / U3 snoRNA-associated protein 13 / U three protein 13
Mass: 91132.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q05946
#29: Protein
U3smallnucleolarRNA-associatedprotein18 / Utp18 / U3 snoRNA-associated protein 18 / U three protein 18
Mass: 66494.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P40362
#30: Protein
U3smallnucleolarRNA-associatedprotein21 / U3 snoRNA-associated protein 21 / U three protein 21
Mass: 104927.844 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q06078
#31: Protein
U3smallnucleolarRNA-associatedprotein6 / Utp6 / U3 snoRNA-associated protein 6 / U three protein 6
Mass: 52495.277 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q02354
#33: Protein
U3smallnucleolarRNA-associatedprotein7 / Utp7 / U3 snoRNA-associated protein 7 / U three protein 7
Mass: 62418.570 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P40055
#34: Protein
U3smallnucleolarRNA-associatedprotein11 / Utp11 / U3 snoRNA-associated protein 11 / U three protein 11
Mass: 29806.348 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P34247
#35: Protein
U3smallnucleolarRNA-associatedproteinMPP10 / Mpp10 / U3 snoRNA-associated protein MPP10 / M phase phosphoprotein 10
Mass: 67042.492 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P47083
#50: Protein
U3smallnucleolarRNA-associatedprotein20 / U3 snoRNA-associated protein 20 / U three protein 20
Mass: 287915.500 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P35194
#51: Protein
U3smallnucleolarRNA-associatedprotein14 / Utp14 / U3 snoRNA-associated protein 14 / U three protein 14
Mass: 103189.750 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: Q04500
+
U3 small nucleolar ribonucleoprotein protein ... , 3 types, 3 molecules 5F5GRB
#36: Protein
U3smallnucleolarribonucleoproteinproteinIMP3 / Imp3 / U3 snoRNP protein IMP3 / Interacting with MPP10 protein 3
Mass: 21928.529 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P32899
#37: Protein
U3smallnucleolarribonucleoproteinproteinIMP4 / Imp4 / U3 snoRNP protein IMP4 / Interacting with MPP10 protein 4
Mass: 33536.168 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / References: UniProt: P53941
Electron dose: 50 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of grids imaged: 10 / Num. of real images: 18028
Image scans
Movie frames/image: 32 / Used frames/image: 1-32
-
Processing
EM software
ID
Name
Version
Category
2
SerialEM
imageacquisition
4
CTFFIND
4.1.8
CTFcorrection
7
UCSF Chimera
modelfitting
8
Coot
modelfitting
10
RELION
initialEulerassignment
11
RELION
finalEulerassignment
12
RELION
classification
13
RELION
3Dreconstruction
CTF correction
Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selection
Num. of particles selected: 382298
3D reconstruction
Resolution: 4.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 9421 / Symmetry type: POINT
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