EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	90 pre-ribosome transformation into the primordial 40 subunit.
ジャーナル・号・ページ	Science, Vol. 369, Issue 6510, Page 1470-1476, Year 2020
掲載日	2020年9月18日
著者	Jingdong Cheng / Benjamin Lau / Giuseppe La Venuta / Michael Ameismeier / Otto Berninghausen / Ed Hurt / Roland Beckmann /
PubMed 要旨	Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this ...Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this process by biochemical and cryo-electron microscopy analysis of intermediates along this pathway in yeast. First, the remodeling RNA helicase Dhr1 engages the 90 pre-ribosome, followed by Utp24 endonuclease-driven RNA cleavage at site A, thereby separating the 5'-external transcribed spacer (ETS) from 18 ribosomal RNA. Next, the 5'-ETS and 90 assembly factors become dislodged, but this occurs sequentially, not en bloc. Eventually, the primordial pre-40 emerges, still retaining some 90 factors including Dhr1, now ready to unwind the final small nucleolar U3-18 RNA hybrid. Our data shed light on the elusive 90 to pre-40 transition and clarify the principles of assembly and remodeling of large ribonucleoproteins.
リンク	Science / PubMed:32943521
手法	EM (単粒子)
解像度	3.5 - 7.1 Å
構造データ	11357 6zqa EMDB-11357, PDB-6zqa: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state A (Poly-Ala) 手法: EM (単粒子) / 解像度: 4.4 Å 11358 6zqb EMDB-11358, PDB-6zqb: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state B2 手法: EM (単粒子) / 解像度: 3.9 Å 11359 6zqc EMDB-11359, PDB-6zqc: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Pre-A1 手法: EM (単粒子) / 解像度: 3.8 Å 11360 6zqd EMDB-11360, PDB-6zqd: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Post-A1 手法: EM (単粒子) / 解像度: 3.8 Å 11361 6zqe EMDB-11361, PDB-6zqe: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Dis-A (Poly-Ala) 手法: EM (単粒子) / 解像度: 7.1 Å 11362 6zqf EMDB-11362, PDB-6zqf: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Dis-B (Poly-Ala) 手法: EM (単粒子) / 解像度: 4.9 Å 11363 6zqg EMDB-11363, PDB-6zqg: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Dis-C 手法: EM (単粒子) / 解像度: 3.5 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子*YM
由来	saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) saccharomyces cerevisiae s288c (パン酵母) accharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
キーワード	RIBOSOME / 90S pre-ribosome / 40S pre-ribosome / A1 cleavage / Dhr1