[English] 日本語
Yorodumi
- EMDB-11362: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cere... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-11362
TitleCryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Dis-B (Poly-Ala)
Map data
Sample
  • Complex: 90S pre-ribbosome state Dis-B
    • Protein or peptide: x 38 types
    • RNA: x 3 types
  • Ligand: x 1 types
Function / homology
Function and homology information


18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / Noc4p-Nop14p complex / regulation of ribosomal protein gene transcription by RNA polymerase II / rRNA small subunit pseudouridine methyltransferase Nep1 / CURI complex / UTP-C complex / Pwp2p-containing subcomplex of 90S preribosome / nuclear microtubule / Mpp10 complex ...18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / Noc4p-Nop14p complex / regulation of ribosomal protein gene transcription by RNA polymerase II / rRNA small subunit pseudouridine methyltransferase Nep1 / CURI complex / UTP-C complex / Pwp2p-containing subcomplex of 90S preribosome / nuclear microtubule / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / regulation of rRNA processing / rRNA (adenine-N6,N6-)-dimethyltransferase activity / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA modification / septum digestion after cytokinesis / tRNA export from nucleus / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA base methylation / single-stranded telomeric DNA binding / rRNA primary transcript binding / sno(s)RNA-containing ribonucleoprotein complex / mTORC1-mediated signalling / Protein hydroxylation / rRNA methylation / U3 snoRNA binding / : / poly(A)+ mRNA export from nucleus / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / snoRNA binding / establishment of cell polarity / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / nucleolar large rRNA transcription by RNA polymerase I / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / enzyme activator activity / proteasome assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / RNA endonuclease activity / nuclear periphery / small-subunit processome / helicase activity / maintenance of translational fidelity / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / rRNA processing / cytosolic small ribosomal subunit / unfolded protein binding / ribosome biogenesis / small ribosomal subunit / cytoplasmic translation / RNA helicase activity / rRNA binding / RNA helicase / ribosome / structural constituent of ribosome / cell cycle / translation / mRNA binding / GTPase activity / GTP binding / nucleolus / ATP hydrolysis activity / mitochondrion / RNA binding / nucleoplasm / ATP binding / identical protein binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Nucleolar complex protein 4 / Nucleolar protein 14 / Nop14-like family / NOL6/Upt22 / Nrap protein domain 1 / Nrap protein, domain 2 / Nrap protein, domain 3 / Nrap protein, domain 4 / Nrap protein, domain 5 / Nrap protein, domain 6 ...Nucleolar complex protein 4 / Nucleolar protein 14 / Nop14-like family / NOL6/Upt22 / Nrap protein domain 1 / Nrap protein, domain 2 / Nrap protein, domain 3 / Nrap protein, domain 4 / Nrap protein, domain 5 / Nrap protein, domain 6 / Nrap protein domain 1 / Nrap protein PAP/OAS-like domain / Nrap protein domain 3 / Nrap protein nucleotidyltransferase domain 4 / Nrap protein PAP/OAS1-like domain 5 / Nrap protein domain 6 / Ribosomal RNA-processing protein 7, C-terminal domain / Ribosomal RNA-processing protein 7 / Rrp7, RRM-like N-terminal domain / Ribosomal RNA-processing protein 7 (RRP7) C-terminal domain / Rrp7 RRM-like N-terminal domain / Sas10 C-terminal domain / Sas10 C-terminal domain / RNA 3'-terminal phosphate cyclase-like, conserved site / Ribosome biogenesis protein Bms1, N-terminal / RNA 3'-terminal phosphate cyclase signature. / U3 small nucleolar RNA-associated protein 13, C-terminal / Periodic tryptophan protein 2 / Utp13 specific WD40 associated domain / Small-subunit processome, Utp12 / Dip2/Utp12 Family / Sas10/Utp3/C1D / Small-subunit processome, Utp21 / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / RNA 3'-terminal phosphate cyclase type 2 / Sas10/Utp3/C1D family / Mpp10 protein / Utp21 specific WD40 associated putative domain / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / EMG1/NEP1 methyltransferase / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase, insert domain / RNA 3'-terminal phosphate cyclase domain / RNA 3'-terminal phosphate cyclase, insert domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase (RTC), insert domain / Ribosomal RNA adenine dimethylase / CCAAT-binding factor / CBF/Mak21 family / Krr1, KH1 domain / Krr1 KH1 domain / Bystin / Bystin / Ribosomal RNA adenine methylase transferase, conserved site / Ribosomal RNA adenine methylase transferase, N-terminal / Ribosomal RNA adenine dimethylases signature. / Ribosomal RNA adenine dimethylases / Ribosomal RNA adenine methyltransferase KsgA/Erm / Ribosomal RNA adenine dimethylase / rRNA adenine N(6)-methyltransferase family profile. / U3 snoRNP protein/Ribosome production factor 1 / Ribosome biogenesis protein BMS1/TSR1, C-terminal / AARP2CN / Bms1/Tsr1-type G domain / Ribosome biogenesis protein Bms1/Tsr1 / 40S ribosome biogenesis protein Tsr1 and BMS1 C-terminal / AARP2CN (NUC121) domain / Bms1-type guanine nucleotide-binding (G) domain profile. / AARP2CN (NUC121) domain / Protein of unknown function (DUF663) / : / Helicase associated domain (HA2), ratchet-like / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase-associated domain / Helicase associated domain (HA2), winged-helix / Helicase associated domain (HA2) Add an annotation / Brix domain / Brix domain / Brix domain profile. / Brix / Anaphase-promoting complex subunit 4, WD40 domain / RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta / Anaphase-promoting complex subunit 4 WD40 domain / tRNA (guanine-N1-)-methyltransferase, N-terminal / Alpha/beta knot methyltransferases / K Homology domain, type 1 superfamily / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / DEAH-box subfamily ATP-dependent helicases signature. / Quinoprotein alcohol dehydrogenase-like superfamily / Ribosomal protein S19e, conserved site / Ribosomal protein S25 / : / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S8e, conserved site
Similarity search - Domain/homology
Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A ...Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Ribosomal RNA-processing protein 7 / Periodic tryptophan protein 2 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7A / U3 small nucleolar ribonucleoprotein protein IMP3 / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein eS27A / Essential nuclear protein 1 / Dimethyladenosine transferase / U3 small nucleolar RNA-associated protein MPP10 / U3 small nucleolar RNA-associated protein 22 / U3 small nucleolar ribonucleoprotein protein IMP4 / Probable ATP-dependent RNA helicase DHR1 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / Nucleolar complex protein 4 / RNA 3'-terminal phosphate cyclase-like protein / Ribosome biogenesis protein BMS1 / Something about silencing protein 10 / U3 small nucleolar RNA-associated protein 12 / Small ribosomal subunit protein eS25A / Small ribosomal subunit protein eS28A / Nucleolar complex protein 14 / Pre-rRNA-processing protein PNO1
Similarity search - Component
Biological speciesaccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) / Baker's yeast (brewer's yeast) / Saccharomyces cerevisiae S288C (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.9 Å
AuthorsCheng J / Lau B / Venuta GL / Berninghausen O / Hurt E / Beckmann R
CitationJournal: Science / Year: 2020
Title: 90 pre-ribosome transformation into the primordial 40 subunit.
Authors: Jingdong Cheng / Benjamin Lau / Giuseppe La Venuta / Michael Ameismeier / Otto Berninghausen / Ed Hurt / Roland Beckmann /
Abstract: Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this ...Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this process by biochemical and cryo-electron microscopy analysis of intermediates along this pathway in yeast. First, the remodeling RNA helicase Dhr1 engages the 90 pre-ribosome, followed by Utp24 endonuclease-driven RNA cleavage at site A, thereby separating the 5'-external transcribed spacer (ETS) from 18 ribosomal RNA. Next, the 5'-ETS and 90 assembly factors become dislodged, but this occurs sequentially, not en bloc. Eventually, the primordial pre-40 emerges, still retaining some 90 factors including Dhr1, now ready to unwind the final small nucleolar U3-18 RNA hybrid. Our data shed light on the elusive 90 to pre-40 transition and clarify the principles of assembly and remodeling of large ribonucleoproteins.
History
DepositionJul 9, 2020-
Header (metadata) releaseSep 23, 2020-
Map releaseSep 23, 2020-
UpdateSep 30, 2020-
Current statusSep 30, 2020Processing site: PDBe / Status: Released

-
Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.01
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.01
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-6zqf
  • Surface level: 0.01
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_11362.png

Downloads & links

-
Map

FileDownload / File: emd_11362.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.059 Å
Density
Contour LevelBy AUTHOR: 0.01 / Movie #1: 0.01
Minimum - Maximum-0.024460558 - 0.053018425
Average (Standard dev.)0.0001849365 (±0.0016468018)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 508.32 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0591.0591.059
M x/y/z480480480
origin x/y/z0.0000.0000.000
length x/y/z508.320508.320508.320
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ100115122
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS480480480
D min/max/mean-0.0240.0530.000

-
Supplemental data

-
Sample components

+
Entire : 90S pre-ribbosome state Dis-B

EntireName: 90S pre-ribbosome state Dis-B
Components
  • Complex: 90S pre-ribbosome state Dis-B
    • Protein or peptide: Periodic tryptophan protein 2
    • Protein or peptide: Nucleolar complex protein 14
    • Protein or peptide: Something about silencing protein 10
    • Protein or peptide: U3 small nucleolar RNA-associated protein 12
    • Protein or peptide: U3 small nucleolar RNA-associated protein 13
    • Protein or peptide: Nucleolar complex protein 4
    • Protein or peptide: U3 small nucleolar RNA-associated protein 21
    • Protein or peptide: U3 small nucleolar RNA-associated protein 22
    • Protein or peptide: U3 small nucleolar ribonucleoprotein protein IMP3
    • Protein or peptide: U3 small nucleolar ribonucleoprotein protein IMP4
    • Protein or peptide: U3 small nucleolar RNA-associated protein MPP10
    • Protein or peptide: Ribosome biogenesis protein BMS1
    • Protein or peptide: RNA 3'-terminal phosphate cyclase-like protein
    • Protein or peptide: Ribosomal RNA-processing protein 7
    • Protein or peptide: Probable ATP-dependent RNA helicase DHR1
    • Protein or peptide: Ribosomal RNA small subunit methyltransferase NEP1
    • Protein or peptide: Essential nuclear protein 1
    • Protein or peptide: Dimethyladenosine transferase
    • Protein or peptide: Pre-rRNA-processing protein PNO1
    • Protein or peptide: Rps5p
    • Protein or peptide: 40S ribosomal protein S16-ARibosome
    • Protein or peptide: 40S ribosomal protein S18-ARibosome
    • Protein or peptide: 40S ribosomal protein S19-ARibosome
    • Protein or peptide: 40S ribosomal protein S28-ARibosome
    • RNA: 5ETS RNA
    • RNA: 18S rRNA18S ribosomal RNA
    • Protein or peptide: 40S ribosomal protein S1-ARibosome
    • Protein or peptide: 40S ribosomal protein S4-ARibosome
    • Protein or peptide: 40S ribosomal protein S6-ARibosome
    • Protein or peptide: 40S ribosomal protein S7-ARibosome
    • Protein or peptide: 40S ribosomal protein S8-ARibosome
    • Protein or peptide: 40S ribosomal protein S9-ARibosome
    • Protein or peptide: 40S ribosomal protein S11-ARibosome
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: 40S ribosomal protein S14-ARibosome
    • Protein or peptide: 40S ribosomal protein S25-ARibosome
    • Protein or peptide: 40S ribosomal protein S22-ARibosome
    • Protein or peptide: 40S ribosomal protein S23-ARibosome
    • Protein or peptide: 40S ribosomal protein S24-ARibosome
    • Protein or peptide: 40S ribosomal protein S27-ARibosome
    • RNA: U3 snoRNA
  • Ligand: ZINC ION

+
Supramolecule #1: 90S pre-ribbosome state Dis-B

SupramoleculeName: 90S pre-ribbosome state Dis-B / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#41
Source (natural)Organism: accharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

+
Macromolecule #1: Periodic tryptophan protein 2

MacromoleculeName: Periodic tryptophan protein 2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 104.097039 KDa
SequenceString: MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF EYEHRKNIAA IDLNKQGTLL ISIDEDGRAI LVNFKARNV LHHFNFKEKC SAVKFSPDGR LFALASGRFL QIWKTPDVNK DRQFAPFVRH RVHAGHFQDI TSLTWSQDSR F ILTTSKDL ...String:
MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF EYEHRKNIAA IDLNKQGTLL ISIDEDGRAI LVNFKARNV LHHFNFKEKC SAVKFSPDGR LFALASGRFL QIWKTPDVNK DRQFAPFVRH RVHAGHFQDI TSLTWSQDSR F ILTTSKDL SAKIWSVDSE EKNLAATTFN GHRDYVMGAF FSHDQEKIYT VSKDGAVFVW EFTKRPSDDD DNESEDDDKQ EE VDISKYS WRITKKHFFY ANQAKVKCVT FHPATRLLAV GFTSGEFRLY DLPDFTLIQQ LSMGQNPVNT VSVNQTGEWL AFG SSKLGQ LLVYEWQSES YILKQQGHFD STNSLAYSPD GSRVVTASED GKIKVWDITS GFCLATFEEH TSSVTAVQFA KRGQ VMFSS SLDGTVRAWD LIRYRNFRTF TGTERIQFNC LAVDPSGEVV CAGSLDNFDI HVWSVQTGQL LDALSGHEGP VSCLS FSQE NSVLASASWD KTIRIWSIFG RSQQVEPIEV YSDVLALSMR PDGKEVAVST LKGQISIFNI EDAKQVGNID CRKDII SGR FNQDRFTAKN SERSKFFTTI HYSFDGMAIV AGGNNNSICL YDVPNEVLLK RFIVSRNMAL NGTLEFLNSK KMTEAGS LD LIDDAGENSD LEDRIDNSLP GSQRGGDLST RKMRPEVRVT SVQFSPTANA FAAASTEGLL IYSTNDTILF DPFDLDVD V TPHSTVEALR EKQFLNALVM AFRLNEEYLI NKVYEAIPIK EIPLVASNIP AIYLPRILKF IGDFAIESQH IEFNLIWIK ALLSASGGYI NEHKYLFSTA MRSIQRFIVR VAKEVVNTTT DNKYTYRFLV STDGSMEDGA ADDDEVLLKD DADEDNEENE ENDVVMESD DEEGWIGFNG KDNKLPLSNE NDSSDEEENE KELP

+
Macromolecule #2: Nucleolar complex protein 14

MacromoleculeName: Nucleolar complex protein 14 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 94.463195 KDa
SequenceString: MAGSQLKNLK AALKARGLTG QTNVKSKNKK NSKRQAKEYD REEKKKAIAE IREEFNPFEI KAARNKRRDG LPSKTADRIA VGKPGISKQ IGEEQRKRAF EARKMMKNKR GGVIDKRFGE RDKLLTEEEK MLERFTRERQ SQSKRNANLF NLEDDEDDGD M FGDGLTHL ...String:
MAGSQLKNLK AALKARGLTG QTNVKSKNKK NSKRQAKEYD REEKKKAIAE IREEFNPFEI KAARNKRRDG LPSKTADRIA VGKPGISKQ IGEEQRKRAF EARKMMKNKR GGVIDKRFGE RDKLLTEEEK MLERFTRERQ SQSKRNANLF NLEDDEDDGD M FGDGLTHL GQSLSLEDEL ANDEEDFLAS KRFNEDDAEL QQPQRKKTKA EVMKEVIAKS KFYKQERQKA QGIMEDQIDN LD DNFEDVM SELMMTQPKK NPMEPKTDLD KEYDIKVKEL QLDKRAAPSD RTKTEEEKNA EAEEKKRELE QQRLDRMNGM IEL EEGEER GVEDLDDGFW ENEEDYEDDN DGIADSDDDI KFEDQGRDEG FSQILKKKNI SISCPRTHDA LLDQVKKLDL DDHP KIVKN IIKAYQPKLA EGNKEKLGKF TAVLLRHIIF LSNQNYLKNV QSFKRTQNAL ISILKSLSEK YNRELSEECR DYINE MQAR YKKNHFDALS NGDLVFFSII GILFSTSDQY HLVITPALIL MSQFLEQIKF NSLKRIAFGA VLVRIVSQYQ RISKRY IPE VVYFFQKILL TFIVEKENQE KPLDFENIRL DSYELGLPLD VDFTKKRSTI IPLHTLSTMD TEAHPVDQCV SVLLNVM ES LDATISTVWK SLPAFNEIIL PIQQLLSAYT SKYSDFEKPR NILNKVEKLT KFTEHIPLAL QNHKPVSIPT HAPKYEEN F NPDKKSYDPD RTRSEINKMK AQLKKERKFT MKEIRKDAKF EARQRIEEKN KESSDYHAKM AHIVNTINTE EGAEKNKYE RERKLRGGKK

+
Macromolecule #3: Something about silencing protein 10

MacromoleculeName: Something about silencing protein 10 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 70.364398 KDa
SequenceString: MVRKGSNRTK TSEVGDEINP YGLNEVDDFA SKREKVLLGQ STFGDSNKDD DHSLLEDEDE EEVLAMDEDD ESIDEREDEE EEEEEELDG AAAYKKIFGR NLETDQLPEE DEENGMLDNE NAWGSTKGEY YGADDLDDDE AAKEIEKEAL RQQKKHLEEL N MNDYLDEE ...String:
MVRKGSNRTK TSEVGDEINP YGLNEVDDFA SKREKVLLGQ STFGDSNKDD DHSLLEDEDE EEVLAMDEDD ESIDEREDEE EEEEEELDG AAAYKKIFGR NLETDQLPEE DEENGMLDNE NAWGSTKGEY YGADDLDDDE AAKEIEKEAL RQQKKHLEEL N MNDYLDEE EEEEWVKSAK EFDMGEFKNS TKQADTKTSI TDILNMDDEA RDNYLRTMFP EFAPLSKEFT ELAPKFDELK KS EENEFNK LKLIALGSYL GTISCYYSIL LHELHNNEDF TSMKGHPVME KILTTKEIWR QASELPSSFD VNEGDGSESE ETA NIEAFN EKKLNELQNS EDSDAEDGGK QKQEIDEEER ESDEEEEEED VDIDDFEEYV AQSRLHSKPK TSSMPEADDF IESE IADVD AQDKKARRRT LRFYTSKIDQ QENKKTDRFK GDDDIPYKER LFERQQRLLD EARKRGMHDN NGADLDDKDY GSEDE AVSR SINTQGENDY YQQVQRGKQD KKISRKEAHK NAVIAAREGK LAELAENVSG DGKRAINYQI LKNKGLTPKR NKDNRN SRV KKRKKYQKAQ KKLKSVRAVY SGGQSGVYEG EKTGIKKGLT RSVKFKN

+
Macromolecule #4: U3 small nucleolar RNA-associated protein 12

MacromoleculeName: U3 small nucleolar RNA-associated protein 12 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 106.481133 KDa
SequenceString: MVKSYQRFEQ AAAFGVIASN ANCVWIPASS GNSNGSGPGQ LITSALEDVN IWDIKTGDLV SKLSDGLPPG ASDARGAKPA ECTYLEAHK DTDLLAVGYA DGVIKVWDLM SKTVLLNFNG HKAAITLLQF DGTGTRLISG SKDSNIIVWD LVGEVGLYKL R SHKDSITG ...String:
MVKSYQRFEQ AAAFGVIASN ANCVWIPASS GNSNGSGPGQ LITSALEDVN IWDIKTGDLV SKLSDGLPPG ASDARGAKPA ECTYLEAHK DTDLLAVGYA DGVIKVWDLM SKTVLLNFNG HKAAITLLQF DGTGTRLISG SKDSNIIVWD LVGEVGLYKL R SHKDSITG FWCQGEDWLI STSKDGMIKL WDLKTHQCIE THIAHTGECW GLAVKDDLLI TTGTDSQVKI WKLDIENDKM GG KLTEMGI FEKQSKQRGL KIEFITNSSD KTSFFYIQNA DKTIETFRIR KEEEIARGLK KREKRLKEKG LTEEEIAKSI KES YSSFIL HPFQTIRSLY KIKSASWTTV SSSKLELVLT TSSNTIEYYS IPYEKRDPTS PAPLKTHTIE LQGQRTDVRS IDIS DDNKL LATASNGSLK IWNIKTHKCI RTFECGYALT CKFLPGGLLV ILGTRNGELQ LFDLASSSLL DTIEDAHDAA IWSLD LTSD GKRLVTGSAD KTVKFWDFKV ENSLVPGTKN KFLPVLKLHH DTTLELTDDI LCVRVSPDDR YLAISLLDNT VKVFFL DSM KFYLSLYGHK LPVLSIDISF DSKMIITSSA DKNIKIWGLD FGDCHKSLFA HQDSIMNVKF LPQSHNFFSC SKDAVVK YW DGEKFECIQK LYAHQSEVWA LAVATDGGFV VSSSHDHSIR IWEETEDQVF LEEEKEKELE EQYEDTLLTS LEEGNGDD A FKADASGEGV EDEASGVHKQ TLESLKAGER LMEALDLGIA EIEGLEAYNR DMKLWQRKKL GEAPIKPQGN AVLIAVNKT PEQYIMDTLL RIRMSQLEDA LMVMPFSYVL KFLKFIDTVM QNKTLLHSHL PLICKNLFFI IKFNHKELVS QKNEELKLQI NRVKTELRS ALKSTEDDLG FNVQGLKFVK QQWNLRHNYE FVDEYDQQEK ESNSARKRVF GTVI

+
Macromolecule #5: U3 small nucleolar RNA-associated protein 13

MacromoleculeName: U3 small nucleolar RNA-associated protein 13 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 91.132562 KDa
SequenceString: MDLKTSYKGI SLNPIYAGSS AVATVSENGK ILATPVLDEI NIIDLTPGSR KILHKISNED EQEITALKLT PDGQYLTYVS QAQLLKIFH LKTGKVVRSM KISSPSYILD ADSTSTLLAV GGTDGSIIVV DIENGYITHS FKGHGGTISS LKFYGQLNSK I WLLASGDT ...String:
MDLKTSYKGI SLNPIYAGSS AVATVSENGK ILATPVLDEI NIIDLTPGSR KILHKISNED EQEITALKLT PDGQYLTYVS QAQLLKIFH LKTGKVVRSM KISSPSYILD ADSTSTLLAV GGTDGSIIVV DIENGYITHS FKGHGGTISS LKFYGQLNSK I WLLASGDT NGMVKVWDLV KRKCLHTLQE HTSAVRGLDI IEVPDNDEPS LNLLSGGRDD IINLWDFNMK KKCKLLKTLP VN QQVESCG FLKDGDGKRI IYTAGGDAIF QLIDSESGSV LKRTNKPIEE LFIIGVLPIL SNSQMFLVLS DQTLQLINVE EDL KNDEDT IQVTSSIAGN HGIIADMRYV GPELNKLALA TNSPSLRIIP VPDLSGPEAS LPLDVEIYEG HEDLLNSLDA TEDG LWIAT ASKDNTAIVW RYNENSCKFD IYAKYIGHSA AVTAVGLPNI VSKGYPEFLL TASNDLTIKK WIIPKPTASM DVQII KVSE YTRHAHEKDI NALSVSPNDS IFATASYDKT CKIWNLENGE LEATLANHKR GLWDVSFCQY DKLLATSSGD KTVKIW SLD TFSVMKTLEG HTNAVQRCSF INKQKQLISC GADGLIKIWD CSSGECLKTL DGHNNRLWAL STMNDGDMIV SADADGV FQ FWKDCTEQEI EEEQEKAKLQ VEQEQSLQNY MSKGDWTNAF LLAMTLDHPM RLFNVLKRAL GESRSRQDTE EGKIEVIF N EELDQAISIL NDEQLILLMK RCRDWNTNAK THTIAQRTIR CILMHHNIAK LSEIPGMVKI VDAIIPYTQR HFTRVDNLV EQSYILDYAL VEMDKLF

+
Macromolecule #6: Nucleolar complex protein 4

MacromoleculeName: Nucleolar complex protein 4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 63.707844 KDa
SequenceString: MVLLISEIKD IAKRLTAAGD RKQYNSIIKL INELVIPENV TQLEEDETEK NLRFLVMSLF QIFRKLFSRG DLTLPSSKKS TLEKEQFVN WCRKVYEAFK TKLLAIISDI PFETSLGLDS LDVYLQLAEL ESTHFASEKG APFFPNKTFR KLIIALWSSN M GEIEDVKS ...String:
MVLLISEIKD IAKRLTAAGD RKQYNSIIKL INELVIPENV TQLEEDETEK NLRFLVMSLF QIFRKLFSRG DLTLPSSKKS TLEKEQFVN WCRKVYEAFK TKLLAIISDI PFETSLGLDS LDVYLQLAEL ESTHFASEKG APFFPNKTFR KLIIALWSSN M GEIEDVKS SGASENLIIV EFTEKYYTKF ADIQYYFQSE FNQLLEDPAY QDLLLKNVGK WLALVNHDKH CSSVDADLEI FV PNPPQAI ENESKFKSNF EKNWLSLLNG QLSLQQYKSI LLILHKRIIP HFHTPTKLMD FLTDSYNLQS SNKNAGVVPI LAL NGLFEL MKRFNLEYPN FYMKLYQIIN PDLMHVKYRA RFFRLMDVFL SSTHLSAHLV ASFIKKLARL TLESPPSAIV TVIP FIYNL IRKHPNCMIM LHNPAFISNP FQTPDQVANL KTLKENYVDP FDVHESDPEL THALDSSLWE LASLMEHYHP NVATL AKIF AQPFKKLSYN MEDFLDWNYD SLLNAESSRK LKTLPTLEFE AFTNVFDNED GDSEASSQGN VYLPGVAW

+
Macromolecule #7: U3 small nucleolar RNA-associated protein 21

MacromoleculeName: U3 small nucleolar RNA-associated protein 21 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 104.927844 KDa
SequenceString: MSIDLKKRKV EEDVRSRGKN SKIFSPFRII GNVSNGVPFA TGTLGSTFYI VTCVGKTFQI YDANTLHLLF VSEKETPSSI VALSAHFHY VYAAYENKVG IYKRGIEEHL LELETDANVE HLCIFGDYLC ASTDDNSIFI YKKSDPQDKY PSEFYTKLTV T EIQGGEIV ...String:
MSIDLKKRKV EEDVRSRGKN SKIFSPFRII GNVSNGVPFA TGTLGSTFYI VTCVGKTFQI YDANTLHLLF VSEKETPSSI VALSAHFHY VYAAYENKVG IYKRGIEEHL LELETDANVE HLCIFGDYLC ASTDDNSIFI YKKSDPQDKY PSEFYTKLTV T EIQGGEIV SLQHLATYLN KLTVVTKSNV LLFNVRTGKL VFTSNEFPDQ ITTAEPAPVL DIIALGTVTG EVIMFNMRKG KR IRTIKIP QSRISSLSFR TDGSSHLSVG TSSGDLIFYD LDRRSRIHVL KNIHRESYGG VTQATFLNGQ PIIVTSGGDN SLK EYVFDP SLSQGSGDVV VQPPRYLRSR GGHSQPPSYI AFADSQSHFM LSASKDRSLW SFSLRKDAQS QEMSQRLHKK QDGG RVGGS TIKSKFPEIV ALAIENARIG EWENIITAHK DEKFARTWDM RNKRVGRWTF DTTDDGFVKS VAMSQCGNFG FIGSS NGSI TIYNMQSGIL RKKYKLHKRA VTGISLDGMN RKMVSCGLDG IVGFYDFNKS TLLGKLKLDA PITAMVYHRS SDLFAL ALD DLSIVVIDAV TQRVVRQLWG HSNRITAFDF SPEGRWIVSA SLDSTIRTWD LPTGGCIDGI IVDNVATNVK FSPNGDL LA TTHVTGNGIC IWTNRAQFKT VSTRTIDESE FARMALPSTS VRGNDSMLSG ALESNGGEDL NDIDFNTYTS LEQIDKEL L TLSIGPRSKM NTLLHLDVIR KRSKPKEAPK KSEKLPFFLQ LSGEKVGDEA SVREGIAHET PEEIHRRDQE AQKKLDAEE QMNKFKVTGR LGFESHFTKQ LREGSQSKDY SSLLATLINF SPAAVDLEIR SLNSFEPFDE IVWFIDALTQ GLKSNKNFEL YETFMSLLF KAHGDVIHAN NKNQDIASAL QNWEDVHKKE DRLDDLVKFC MGVAAFVTTA

+
Macromolecule #8: U3 small nucleolar RNA-associated protein 22

MacromoleculeName: U3 small nucleolar RNA-associated protein 22 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 140.660141 KDa
SequenceString: MATSVKRKAS ETSDQNIVKV QKKHSTQDST TDNGSKENDH SSQAINERTV PEQENDESDT SPESNEVATN TAATRHNGKV TATESYDIH IARETAELFK SNIFKLQIDE LLEQVKLKQK HVLKVEKFLH KLYDILQEIP DWEEKSLAEV DSFFKNKIVS V PFVDPKPI ...String:
MATSVKRKAS ETSDQNIVKV QKKHSTQDST TDNGSKENDH SSQAINERTV PEQENDESDT SPESNEVATN TAATRHNGKV TATESYDIH IARETAELFK SNIFKLQIDE LLEQVKLKQK HVLKVEKFLH KLYDILQEIP DWEEKSLAEV DSFFKNKIVS V PFVDPKPI PQNTNYKFNY KKPDISLIGS FALKAGIYQP NGSSIDTLLT MPKELFEKKD FLNFRCLHKR SVYLAYLTHH LL ILLKKDK LDSFLQLEYS YFDNDPLLPI LRISCSKPTG DSLSDYNFYK TRFSINLLIG FPYKVFEPKK LLPNRNCIRI AQE SKEQSL PATPLYNFSV LSSSTHENYL KYLYKTKKQT ESFVEATVLG RLWLQQRGFS SNMSHSGSLG GFGTFEFTIL MAAL LNGGG INSNKILLHG FSSYQLFKGV IKYLATMDLC HDGHLQFHSN PENSSSSPAS KYIDEGFQTP TLFDKSTKVN ILTKM TVSS YQILKEYAGE TLRMLNNVVQ DQFSNIFLTN ISRFDNLKYD LCYDVQLPLG KYNNLETSLA ATFGSMERVK FITLEN FLA HKITNVARYA LGDRIKYIQI EMVGQKSDFP ITKRKVYSNT GGNHFNFDFV RVKLIVNPSE CDKLVTKGPA HSETMST EA AVFKNFWGIK SSLRRFKDGS ITHCCVWSTS SSEPIISSIV NFALQKHVSK KAQISNETIK KFHNFLPLPN LPSSAKTS V LNLSSFFNLK KSFDDLYKII FQMKLPLSVK SILPVGSAFR YTSLCQPVPF AYSDPDFFQD VILEFETSPK WPDEITSLE KAKTAFLLKI QEELSANSST YRSFFSRDES IPYNLEIVTL NILTPEGYGF KFRVLTERDE ILYLRAIANA RNELKPELEA TFLKFTAKY LASVRHTRTL ENISHSYQFY SPVVRLFKRW LDTHLLLGHI TDELAELIAI KPFVDPAPYF IPGSLENGFL K VLKFISQW NWKDDPLILD LVKPEDDIRD TFETSIGAGS ELDSKTMKKL SERLTLAQYK GIQMNFTNLR NSDPNGTHLQ FF VASKNDP SGILYSSGIP LPIATRLTAL AKVAVNLLQT HGLNQQTINL LFTPGLKDYD FVVDLRTPIG LKSSCGILSA TEF KNITND QAPSNFPENL NDLSEKMDPT YQLVKYLNLK YKNSLILSSR KYIGVNGGEK GDKNVITGLI KPLFKGAHKF RVNL DCNVK PVDDENVILN KEAIFHEIAA FGNDMVINFE TD

+
Macromolecule #9: U3 small nucleolar ribonucleoprotein protein IMP3

MacromoleculeName: U3 small nucleolar ribonucleoprotein protein IMP3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.928529 KDa
SequenceString:
MVRKLKHHEQ KLLKKVDFLE WKQDQGHRDT QVMRTYHIQN REDYHKYNRI CGDIRRLANK LSLLPPTDPF RRKHEQLLLD KLYAMGVLT TKSKISDLEN KVTVSAICRR RLPVIMHRLK MAETIQDAVK FIEQGHVRVG PNLINDPAYL VTRNMEDYVT W VDNSKIKK TLLRYRNQID DFDFS

+
Macromolecule #10: U3 small nucleolar ribonucleoprotein protein IMP4

MacromoleculeName: U3 small nucleolar ribonucleoprotein protein IMP4 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 33.536168 KDa
SequenceString: MLRRQARERR EYLYRKAQEL QDSQLQQKRQ IIKQALAQGK PLPKELAEDE SLQKDFRYDQ SLKESEEADD LQVDDEYAAT SGIMDPRII VTTSRDPSTR LSQFAKEIKL LFPNAVRLNR GNYVMPNLVD ACKKSGTTDL VVLHEHRGVP TSLTISHFPH G PTAQFSLH ...String:
MLRRQARERR EYLYRKAQEL QDSQLQQKRQ IIKQALAQGK PLPKELAEDE SLQKDFRYDQ SLKESEEADD LQVDDEYAAT SGIMDPRII VTTSRDPSTR LSQFAKEIKL LFPNAVRLNR GNYVMPNLVD ACKKSGTTDL VVLHEHRGVP TSLTISHFPH G PTAQFSLH NVVMRHDIIN AGNQSEVNPH LIFDNFTTAL GKRVVCILKH LFNAGPKKDS ERVITFANRG DFISVRQHVY VR TREGVEI AEVGPRFEMR LFELRLGTLE NKDADVEWQL RRFIRTANKK DYL

+
Macromolecule #11: U3 small nucleolar RNA-associated protein MPP10

MacromoleculeName: U3 small nucleolar RNA-associated protein MPP10 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 67.042492 KDa
SequenceString: MSELFGVLKS NAGRIILKDP SATSKDVKAY IDSVINTCKN GSITKKAELD EITVDGLDAN QVWWQVKLVL DSIDGDLIQG IQELKDVVT PSHNLSDGST LNSSSGEESE LEEAESVFKE KQMLSADVSE IEEQSNDSLS ENDEEPSMDD EKTSAEAARE E FAEEKRIS ...String:
MSELFGVLKS NAGRIILKDP SATSKDVKAY IDSVINTCKN GSITKKAELD EITVDGLDAN QVWWQVKLVL DSIDGDLIQG IQELKDVVT PSHNLSDGST LNSSSGEESE LEEAESVFKE KQMLSADVSE IEEQSNDSLS ENDEEPSMDD EKTSAEAARE E FAEEKRIS SGQDERHSSP DPYGINDKFF DLEKFNRDTL AAEDSNEASE GSEDEDIDYF QDMPSDDEEE EAIYYEDFFD KP TKEPVKK HSDVKDPKED EELDEEEHDS AMDKVKLDLF ADEEDEPNAE GVGEASDKNL SSFEKQQIEI RKQIEQLENE AVA EKKWSL KGEVKAKDRP EDALLTEELE FDRTAKPVPV ITSEVTESLE DMIRRRIQDS NFDDLQRRTL LDITRKSQRP QFEL SDVKS SKSLAEIYED DYTRAEDESA LSEELQKAHS EISELYANLV YKLDVLSSVH FVPKPASTSL EIRVETPTIS MEDAQ PLYM SNASSLAPQE IYNVGKAEKD GEIRLKNGVA MSKEELTRED KNRLRRALKR KRSKANLPNV NKRSKRNDVV DTLSKA KNI TVINQKGEKK DVSGKTKKSR SGPDSTNIKL

+
Macromolecule #12: Ribosome biogenesis protein BMS1

MacromoleculeName: Ribosome biogenesis protein BMS1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 135.792281 KDa
SequenceString: MEQSNKQHRK AKEKNTAKKK LHTQGHNAKA FAVAAPGKMA RTMQRSSDVN ERKLHVPMVD RTPEDDPPPF IVAVVGPPGT GKTTLIRSL VRRMTKSTLN DIQGPITVVS GKHRRLTFLE CPADDLNAMI DIAKIADLVL LLIDGNFGFE METMEFLNIA Q HHGMPRVL ...String:
MEQSNKQHRK AKEKNTAKKK LHTQGHNAKA FAVAAPGKMA RTMQRSSDVN ERKLHVPMVD RTPEDDPPPF IVAVVGPPGT GKTTLIRSL VRRMTKSTLN DIQGPITVVS GKHRRLTFLE CPADDLNAMI DIAKIADLVL LLIDGNFGFE METMEFLNIA Q HHGMPRVL GVATHLDLFK SQSTLRASKK RLKHRFWTEV YQGAKLFYLS GVINGRYPDR EILNLSRFIS VMKFRPLKWR NE HPYMLAD RFTDLTHPEL IETQGLQIDR KVAIYGYLHG TPLPSAPGTR VHIAGVGDFS VAQIEKLPDP CPTPFYQQKL DDF EREKMK EEAKANGEIT TASTTRRRKR LDDKDKLIYA PMSDVGGVLM DKDAVYIDIG KKNEEPSFVP GQERGEGEKL MTGL QSVEQ SIAEKFDGVG LQLFSNGTEL HEVADHEGMD VESGEESIED DEGKSKGRTS LRKPRIYGKP VQEEDADIDN LPSDE EPYT NDDDVQDSEP RMVEIDFNNT GEQGAEKLAL ETDSEFEESE DEFSWERTAA NKLKKTESKK RTWNIGKLIY MDNISP EEC IRRWRGEDDD SKDESDIEED VDDDFFRKKD GTVTKEGNKD HAVDLEKFVP YFDTFEKLAK KWKSVDAIKE RFLGAGI LG NDNKTKSDSN EGGEELYGDF EDLEDGNPSE QAEDNSDKES EDEDENEDTN GDDDNSFTNF DAEEKKDLTM EQEREMNA A KKEKLRAQFE IEEGENFKED DENNEYDTWY ELQKAKISKQ LEINNIEYQE MTPEQRQRIE GFKAGSYVRI VFEKVPMEF VKNFNPKFPI VMGGLLPTEI KFGIVKARLR RHRWHKKILK TNDPLVLSLG WRRFQTLPIY TTTDSRTRTR MLKYTPEHTY CNAAFYGPL CSPNTPFCGV QIVANSDTGN GFRIAATGIV EEIDVNIEIV KKLKLVGFPY KIFKNTAFIK DMFSSAMEVA R FEGAQIKT VSGIRGEIKR ALSKPEGHYR AAFEDKILMS DIVILRSWYP VRVKKFYNPV TSLLLKEKTE WKGLRLTGQI RA AMNLETP SNPDSAYHKI ERVERHFNGL KVPKAVQKEL PFKSQIHQMK PQKKKTYMAK RAVVLGGDEK KARSFIQKVL TIS KAKDSK RKEQKASQRK ERLKKLAKME EEKSQRDKEK KKEYFAQNGK RTTMGGDDES RPRKMRR

+
Macromolecule #13: RNA 3'-terminal phosphate cyclase-like protein

MacromoleculeName: RNA 3'-terminal phosphate cyclase-like protein / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 40.220559 KDa
SequenceString: MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHI CPSSKPVGYF VEPMLYLAPF SKKKFSILFK GITASHNDAG IEAIKWGLMP VMEKFGVREC ALHTLKRGSP P LGGGEVHL ...String:
MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHI CPSSKPVGYF VEPMLYLAPF SKKKFSILFK GITASHNDAG IEAIKWGLMP VMEKFGVREC ALHTLKRGSP P LGGGEVHL VVDSLIAQPI TMHEIDRPII SSITGVAYST RVSPSLVNRM IDGAKKVLKN LQCEVNITAD VWRGENSGKS PG WGITLVA QSKQKGWSYF AEDIGDAGSI PEELGEKVAC QLLEEISKSA AVGRNQLPLA IVYMVIGKED IGRLRINKEQ IDE RFIILL RDIKKIFNTE VFLKPVDEAD NEDMIATIKG IGFTNTSKKI A

+
Macromolecule #14: Ribosomal RNA-processing protein 7

MacromoleculeName: Ribosomal RNA-processing protein 7 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 34.526441 KDa
SequenceString: MGIEDISAMK NGFIVVPFKL PDHKALPKSQ EASLHFMFAK RHQSSNSNES DCLFLVNLPL LSNIEHMKKF VGQLCGKYDT VSHVEELLY NDEFGLHEVD LSALTSDLMS STDVNEKRYT PRNTALLKFV DAASINNCWN ALKKYSNLHA KHPNELFEWT Y TTPSFTTF ...String:
MGIEDISAMK NGFIVVPFKL PDHKALPKSQ EASLHFMFAK RHQSSNSNES DCLFLVNLPL LSNIEHMKKF VGQLCGKYDT VSHVEELLY NDEFGLHEVD LSALTSDLMS STDVNEKRYT PRNTALLKFV DAASINNCWN ALKKYSNLHA KHPNELFEWT Y TTPSFTTF VNFYKPLDID YLKEDIHTHM AIFEQREAQA QEDVQSSIVD EDGFTLVVGK NTKSLNSIRK KILNKNPLSK HE NKAKPIS NIDKKAKKDF YRFQVRERKK QEINQLLSKF KEDQERIKVM KAKRKFNPYT

+
Macromolecule #15: Probable ATP-dependent RNA helicase DHR1

MacromoleculeName: Probable ATP-dependent RNA helicase DHR1 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 145.171406 KDa
SequenceString: MGTYRKRFNE KARSGHMAKL KELKRIRNKQ FTRQDENDER VENPDSAPAE SSTTEPNANA EILEPLTEEE KKMKKRKLQE LFTPKESKV SRLKKKRLDK FIEHQLKREE RKTIIGKLQD YKIDTSLLTS SKRLGEGRQT KKEEFKEALS LERQGRGNEQ T NEILYEEY ...String:
MGTYRKRFNE KARSGHMAKL KELKRIRNKQ FTRQDENDER VENPDSAPAE SSTTEPNANA EILEPLTEEE KKMKKRKLQE LFTPKESKV SRLKKKRLDK FIEHQLKREE RKTIIGKLQD YKIDTSLLTS SKRLGEGRQT KKEEFKEALS LERQGRGNEQ T NEILYEEY EPKVWDEYGE GGSSEDDDGE DDFEASFGSM PKPTDNEEKK SSGFIDHRPA KFGGSGLSFG FSNIKVINKE SK TPKKKYN WRQRVEMEEL KKHGKEDEMD FDTTSEDDDE EEDQEEEDKM HPSENPLEEV ESADSETGSE KFDQNDVANE FKD WANQEI KKLEGRDQEL VTPTLNIDYK PIIRKEDLDD GLQEAYVPIN ENSTRKAFYV EVSRSDEIQK ARIQLPVFGE EHKI MEAIH HNDVVIICGE TGSGKTTQVP QFLYEAGFGA EDSPDYPGMV GITQPRRVAA VSMAERVANE LGDHGHKVGY QIRFD STAK EDTKVKFMTD GVLLREMMHD FKLTKYSSII IDEAHERNIN TDILIGMLSR CVRLRAKLHK ENPIEHKKLK LIIMSA TLR VSDFSENKTL FPIAPPVLQV DARQFPVSIH FNRRTAFNYT DEAFRKTCKI HQKLPPGAIL VFLTGQQEIT HMVKRLR KE FPFKKNSKYN KDLETPVSKM GINSKTTDLE AEDIDFSVQV IDQDKFKSAI RYEEDEGNSG NGEDEEDEEE EGFEEVLT E GQTANDPLYV LPLYSLLPTK EQMRVFQKPP QGSRLCIVAT NVAETSLTIP GVRYVVDSGR SKERKYNESN GVQSFEVGW VSKASANQRS GRAGRTGPGH CYRLYSSAVF EHDFEQFSKP EILRMPVESI VLQMKSMAIH NIINFPFPTP PDRVALSKAI QLLQYLGAL DNKEMITEDG KKMSLFPLSP RFSKMLLVSD EKACLPYIVA IVSALSVGDP FINEFELGIN EISRKPNPDE N LDDKIREH DESTPGMDPE LKKELRSKFY KSRSQFSKLD KFSDVFRLLS VVSAMDYVPK EQKEIFMKKN FLRGKLMEEI VK LRKQLMY IIKSNTSKEN IAVVIRNEDL KSDIPSVIQI KLLKQMICAG FVDHVAVRAD VLFPDDAKIT NRTSIINIPY IPV LATRTP NIEDCFVYIH PTSILNNLGE MPPKYMLYYS LHLGGNNKTR MNTLCDIAST PLANIARKGL LLTYSKPLTG QGLK TVNLS PTERYCYVVP RFGSTVDNDL KIGWDLNPIA VHQKKQKGQW TVIKFITRKG FQTITGEEKE KK

+
Macromolecule #16: Ribosomal RNA small subunit methyltransferase NEP1

MacromoleculeName: Ribosomal RNA small subunit methyltransferase NEP1 / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
EC number: rRNA small subunit pseudouridine methyltransferase Nep1
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 27.936461 KDa
SequenceString: MVEDSRVRDA LKGGDQKALP ASLVPQAPPV LTSKDKITKR MIVVLAMASL ETHKISSNGP GGDKYVLLNC DDHQGLLKKM GRDISEARP DITHQCLLTL LDSPINKAGK LQVYIQTSRG ILIEVNPTVR IPRTFKRFSG LMVQLLHKLS IRSVNSEEKL L KVIKNPIT ...String:
MVEDSRVRDA LKGGDQKALP ASLVPQAPPV LTSKDKITKR MIVVLAMASL ETHKISSNGP GGDKYVLLNC DDHQGLLKKM GRDISEARP DITHQCLLTL LDSPINKAGK LQVYIQTSRG ILIEVNPTVR IPRTFKRFSG LMVQLLHKLS IRSVNSEEKL L KVIKNPIT DHLPTKCRKV TLSFDAPVIR VQDYIEKLDD DESICVFVGA MARGKDNFAD EYVDEKVGLS NYPLSASVAC SK FCHGAED AWNIL

+
Macromolecule #17: Essential nuclear protein 1

MacromoleculeName: Essential nuclear protein 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 55.207422 KDa
SequenceString: MARASSTKAR KQRHDPLLKD LDAAQGTLKK INKKKLAQND AANHDAANEE DGYIDSKASR KILQLAKEQQ DEIEGEELAE SERNKQFEA RFTTMSYDDE DEDEDEDEEA FGEDISDFEP EGDYKEEEEI VEIDEEDAAM FEQYFKKSDD FNSLSGSYNL A DKIMASIR ...String:
MARASSTKAR KQRHDPLLKD LDAAQGTLKK INKKKLAQND AANHDAANEE DGYIDSKASR KILQLAKEQQ DEIEGEELAE SERNKQFEA RFTTMSYDDE DEDEDEDEEA FGEDISDFEP EGDYKEEEEI VEIDEEDAAM FEQYFKKSDD FNSLSGSYNL A DKIMASIR EKESQVEDMQ DDEPLANEQN TSRGNISSGL KSGEGVALPE KVIKAYTTVG SILKTWTHGK LPKLFKVIPS LR NWQDVIY VTNPEEWSPH VVYEATKLFV SNLTAKESQK FINLILLERF RDNIETSEDH SLNYHIYRAV KKSLYKPSAF FKG FLFPLV ETGCNVREAT IAGSVLAKVS VPALHSSAAL SYLLRLPFSP PTTVFIKILL DKKYALPYQT VDDCVYYFMR FRIL DDGSN GEDATRVLPV IWHKAFLTFA QRYKNDITQD QRDFLLETVR QRGHKDIGPE IRRELLAGAS REFVDPQEAN DDLMI DVN

+
Macromolecule #18: Dimethyladenosine transferase

MacromoleculeName: Dimethyladenosine transferase / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
EC number: 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 36.003836 KDa
SequenceString: MGKAAKKKYS GATSSKQVSA EKHLSSVFKF NTDLGQHILK NPLVAQGIVD KAQIRPSDVV LEVGPGTGNL TVRILEQAKN VVAVEMDPR MAAELTKRVR GTPVEKKLEI MLGDFMKTEL PYFDICISNT PYQISSPLVF KLINQPRPPR VSILMFQREF A LRLLARPG ...String:
MGKAAKKKYS GATSSKQVSA EKHLSSVFKF NTDLGQHILK NPLVAQGIVD KAQIRPSDVV LEVGPGTGNL TVRILEQAKN VVAVEMDPR MAAELTKRVR GTPVEKKLEI MLGDFMKTEL PYFDICISNT PYQISSPLVF KLINQPRPPR VSILMFQREF A LRLLARPG DSLYCRLSAN VQMWANVTHI MKVGKNNFRP PPQVESSVVR LEIKNPRPQV DYNEWDGLLR IVFVRKNRTI SA GFKSTTV MDILEKNYKT FLAMNNEMVD DTKGSMHDVV KEKIDTVLKE TDLGDKRAGK CDQNDFLRLL YAFHQVGIHF S

+
Macromolecule #19: Pre-rRNA-processing protein PNO1

MacromoleculeName: Pre-rRNA-processing protein PNO1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 30.380623 KDa
SequenceString: MVAPTALKKA TVTPVSGQDG GSSRIIGINN TESIDEDDDD DVLLDDSDNN TAKEEVEGEE GSRKTHESKT VVVDDQGKPR FTSASKTQG NKIKFESRKI MVPPHRMTPL RNSWTKIYPP LVEHLKLQVR MNLKTKSVEL RTNPKFTTDP GALQKGADFI K AFTLGFDL ...String:
MVAPTALKKA TVTPVSGQDG GSSRIIGINN TESIDEDDDD DVLLDDSDNN TAKEEVEGEE GSRKTHESKT VVVDDQGKPR FTSASKTQG NKIKFESRKI MVPPHRMTPL RNSWTKIYPP LVEHLKLQVR MNLKTKSVEL RTNPKFTTDP GALQKGADFI K AFTLGFDL DDSIALLRLD DLYIETFEVK DVKTLTGDHL SRAIGRIAGK DGKTKFAIEN ATRTRIVLAD SKIHILGGFT HI RMARESV VSLILGSPPG KVYGNLRTVA SRLKERY

+
Macromolecule #20: Rps5p

MacromoleculeName: Rps5p / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 25.0726 KDa
SequenceString: MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV ...String:
MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV NQAIALLTIG AREAAFRNIK TIAETLAEEL INAAKGSSTS YAIKKKDELE RVAKSNR

+
Macromolecule #21: 40S ribosomal protein S16-A

MacromoleculeName: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.87749 KDa
SequenceString:
MSAVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVE PEILRFKVYE PLLLVGLDKF SNIDIRVRVT GGGHVSQVYA IRQAIAKGL VAYHQKYVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGKGARSRFQ KSYR

+
Macromolecule #22: 40S ribosomal protein S18-A

MacromoleculeName: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 17.071641 KDa
SequenceString:
MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ NDITDGKDYH TLANNVESKL RDDLERLKKI RAHRGIRHFW GLRVRGQHTK TTGRRRA

+
Macromolecule #23: 40S ribosomal protein S19-A

MacromoleculeName: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.942125 KDa
SequenceString:
MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVR PYKHIDASGS INRKVLQALE KIGIVEISPK GGRRISENGQ RDLDRIAAQT LEEDE

+
Macromolecule #24: 40S ribosomal protein S28-A

MacromoleculeName: 40S ribosomal protein S28-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 7.605847 KDa
SequenceString:
MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE NDILVLMESE REARRLR

+
Macromolecule #27: 40S ribosomal protein S1-A

MacromoleculeName: 40S ribosomal protein S1-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 28.798467 KDa
SequenceString: MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI ...String:
MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI SEILTKEVQG STLAQLTSKL IPEVINKEIE NATKDIFPLQ NIHVRKVKLL KQPKFDVGAL MALHGEGSGE EK GKKVTGF KDEVLETV

+
Macromolecule #28: 40S ribosomal protein S4-A

MacromoleculeName: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 29.46933 KDa
SequenceString: MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ...String:
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ITDFIKFDAG KLVYVTGGRN LGRIGTIVHK ERHDGGFDLV HIKDSLDNTF VTRLNNVFVI GEQGKPYISL PK GKGIKLS IAEERDRRRA QQGL

+
Macromolecule #29: 40S ribosomal protein S6-A

MacromoleculeName: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 27.054486 KDa
SequenceString: MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT ...String:
MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK RRASSLKA

+
Macromolecule #30: 40S ribosomal protein S7-A

MacromoleculeName: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.658209 KDa
SequenceString:
MSAPQAKILS QAPTELELQV AQAFVELENS SPELKAELRP LQFKSIREID VAGGKKALAI FVPVPSLAGF HKVQTKLTRE LEKKFQDRH VIFLAERRIL PKPSRTSRQV QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKVLLDSKDV Q QIDYKLES FQAVYNKLTG KQIVFEIPSE TH

+
Macromolecule #31: 40S ribosomal protein S8-A

MacromoleculeName: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 22.537803 KDa
SequenceString: MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ...String:
MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

+
Macromolecule #32: 40S ribosomal protein S9-A

MacromoleculeName: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 22.487893 KDa
SequenceString: MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR ...String:
MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR PGRVARRNAA RKAEASGEAA DEADEADEE

+
Macromolecule #33: 40S ribosomal protein S11-A

MacromoleculeName: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 17.785934 KDa
SequenceString:
MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTKM HRTIVIRRA YLHYIPKYNR YEKRHKNVPV HVSPAFRVQV GDIVTVGQCR PISKTVRFNV VKVSAAAGKA NKQFAKF

+
Macromolecule #34: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 17.059945 KDa
SequenceString:
MGRMHSAGKG ISSSAIPYSR NAPAWFKLSS ESVIEQIVKY ARKGLTPSQI GVLLRDAHGV TQARVITGNK IMRILKSNGL APEIPEDLY YLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVAVLPPNW KYESATASAL VN

+
Macromolecule #35: 40S ribosomal protein S14-A

MacromoleculeName: 40S ribosomal protein S14-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.562655 KDa
SequenceString:
MSNVVQARDN SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGT RTKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

+
Macromolecule #36: 40S ribosomal protein S25-A

MacromoleculeName: 40S ribosomal protein S25-A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 12.067272 KDa
SequenceString:
MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGII KPISKHSKQA IYTRATASE

+
Macromolecule #37: 40S ribosomal protein S22-A

MacromoleculeName: 40S ribosomal protein S22-A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.650062 KDa
SequenceString:
MTRSSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIGDIEKW TANLLPARQF GYVILTTSAG IMDHEEARRK HVSGKILGFV Y

+
Macromolecule #38: 40S ribosomal protein S23-A

MacromoleculeName: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 16.073896 KDa
SequenceString:
MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK GIVLEKLGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

+
Macromolecule #39: 40S ribosomal protein S24-A

MacromoleculeName: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.362848 KDa
SequenceString:
MSDAVTIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEVYKA EKDAVSVFGF RTQFGGGKSV GFGLVYNSVA EAKKFEPTY RLVRYGLAEK VEKASRQQRK QKKNRDKKIF GTGKRLAKKV ARRNAD

+
Macromolecule #40: 40S ribosomal protein S27-A

MacromoleculeName: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 8.893391 KDa
SequenceString:
MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK

+
Macromolecule #25: 5ETS RNA

MacromoleculeName: 5ETS RNA / type: rna / ID: 25 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 6.441831 KDa
SequenceString:
GGAUUUGGUG GAUUACUAGC

+
Macromolecule #26: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 26 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 566.062188 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCCGAU UUUUUCGUGU ACUGGAUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUG U GGCUCUUGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGUAU UGCUCGAAUA UAUUAGCAU GGAAUAAUAG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUU CAAUUGUCAG AGGUGAAAUU CUUGGAUUUA UUGAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU U UCAUUAAU CAAGAACGAA AGUUAGGGGA UCGAAGAUGA UCAGAUACCG UCGUAGUCUU AACCAUAAAC UAUGCCGACU AG GGAUCGG GUGGUGUUUU UUUAAUGACC CACUCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGU CGCAAG GCUAAAACUU AAAGGAAUUG ACGGAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGG GAAAC UCACCAGGUC CAGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCC GUUC UUAGUUGGUG GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCA UUU GCUGGUUAUC CACUUCUUAG AGGGACUAUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACAGGUCUG UGAUGCC CU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUU G UGAAACUCCG UCGUGCUGGG GAUAGAGCAU UGUAAUUAUU GCUCUUCAAC GAGGAAUUCC UAGUAAGCGC AAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUUCC AUCUCAGAGC GGAGAAUUUG GACAAACUU GGUCAUUUAG AGGAACUAAA AGUCGUAACA AGGUUUCCGU AGGUGAACCU GCGGAAGGAU CAUUAAAGA

+
Macromolecule #41: U3 snoRNA

MacromoleculeName: U3 snoRNA / type: rna / ID: 41 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 11.187725 KDa
SequenceString:
GUCGACGUAC UUCAAGGAUC AGCCACUGAA UCCAA

+
Macromolecule #42: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 42 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
GridModel: Quantifoil / Material: COPPER / Support film - Material: CARBON
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 44.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Initial angle assignmentType: OTHER / Details: Relion
Final angle assignmentType: OTHER / Details: Relion
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 16654
FSC plot (resolution estimation)

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more