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- EMDB-11893: 43S preinitiation complex from Trypanosoma cruzi with the kDDX60 ... -

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Basic information

Entry
Database: EMDB / ID: EMD-11893
Title43S preinitiation complex from Trypanosoma cruzi with the kDDX60 helicase
Map data
Sample
  • Complex: 43S preinitiation complex from Trypanosoma cruzi with the helicase kDDX60
    • Protein or peptide: x 50 types
    • RNA: x 2 types
  • Ligand: x 3 types
Keywordspreinitiation / factors / kinetoplastid / helicase / TRANSLATION
Function / homology
Function and homology information


eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex / formation of cytoplasmic translation initiation complex / protein-synthesizing GTPase / eukaryotic 43S preinitiation complex / eukaryotic 48S preinitiation complex / regulation of translational initiation / 90S preribosome / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity ...eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex / formation of cytoplasmic translation initiation complex / protein-synthesizing GTPase / eukaryotic 43S preinitiation complex / eukaryotic 48S preinitiation complex / regulation of translational initiation / 90S preribosome / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation initiation factor binding / translation initiation factor activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / modification-dependent protein catabolic process / protein tag activity / rRNA processing / metallopeptidase activity / ribosomal small subunit biogenesis / ribosome binding / kinase activity / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / cytoplasmic translation / nucleic acid binding / tRNA binding / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / translation / GTPase activity / mRNA binding / nucleotide binding / ubiquitin protein ligase binding / GTP binding / RNA binding / zinc ion binding / ATP binding / membrane / nucleus / cytosol / cytoplasm
Similarity search - Function
: / ATP-dependent RNA helicase DDX60, PIN-like domain / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor SUI1 / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain ...: / ATP-dependent RNA helicase DDX60, PIN-like domain / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor SUI1 / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / SUI1 domain superfamily / Translation initiation factor SUI1 / Translation initiation factor SUI1 family profile. / SUI1 domain / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Eukaryotic translation initiation factor 3 subunit A / Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Translation initiation factor IF2/IF5 / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / W2 domain / W2 domain profile. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / : / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / IF2a, S1-like domain / Eukaryotic translation initiation factor 2 alpha subunit / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C terminal / : / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / S1 domain profile. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S30 / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / : / Ribosomal protein S6, eukaryotic / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain
Similarity search - Domain/homology
40S ribosomal protein S33 / Putative 40S ribosomal protein S21 / Ubiquitin-ribosomal protein eL40 fusion protein / Putative 40S ribosomal protein S9 / Putative eukaryotic translation initiation factor 1A / 60S ribosomal protein L41 / 40S ribosomal protein S4 / protein-synthesizing GTPase / Small ribosomal subunit protein uS2 / 40S ribosomal protein S12 ...40S ribosomal protein S33 / Putative 40S ribosomal protein S21 / Ubiquitin-ribosomal protein eL40 fusion protein / Putative 40S ribosomal protein S9 / Putative eukaryotic translation initiation factor 1A / 60S ribosomal protein L41 / 40S ribosomal protein S4 / protein-synthesizing GTPase / Small ribosomal subunit protein uS2 / 40S ribosomal protein S12 / 40S ribosomal protein S7 / Ribosomal protein S29, putative / 40S ribosomal protein S15a, putative / 40S ribosomal protein S26 / 40S ribosomal protein S11, putative / Eukaryotic translation initiation factor 3 (eIF-3) interacting protein, putative / Ribosomal protein S20, putative / 40S ribosomal protein S14, putative / Eukaryotic translation initiation factor 3 subunit E / 40S ribosomal protein S30 / 40S ribosomal protein S13, putative / Eukaryotic translation initiation factor 3 subunit 7-like protein, putative / CSN8/PSMD8/EIF3K domain-containing protein / 40S ribosomal protein S16, putative / 40S ribosomal protein S15, putative / Translation initiation factor, putative / 40S ribosomal protein S2, putative / Ribosomal protein S19, putative / 40S ribosomal protein S17, putative / Eukaryotic translation initiation factor 3 subunit a / Protein translation factor SUI1 homolog, putative / 40S ribosomal protein S3 / Ribosomal protein S27, putative / JAB1/MPN/MOV34 metalloenzyme domain-containing protein / Eukaryotic translation initiation factor 3 subunit h / 40S ribosomal protein S6 / Activated protein kinase C receptor, putative / 40S ribosomal protein S23, putative / 40S ribosomal protein S25 / 40S ribosomal protein S8 / 40S ribosomal protein S24 / RNA-binding protein, putative / Small ribosomal subunit protein eS1B / Eukaryotic translation initiation factor 5, putative / 40S ribosomal protein S10, putative / 40S ribosomal protein S18, putative / 40S ribosomal protein S5, putative / Eukaryotic translation initiation factor 3 subunit 8, putative / ATP-dependent DEAD/H RNA helicase / Elongation initiation factor 2 alpha subunit, putative
Similarity search - Component
Biological speciesTrypanosoma cruzi (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.33 Å
AuthorsBochler A / Brito Querido J
Funding support France, Czech Republic, 4 items
OrganizationGrant numberCountry
European Research Council (ERC)ERC-2017-STG#759120TransTryp France
Laboratories of Excellence (LabEx)ANR10LABX0036NETRNA France
Laboratories of Excellence (LabEx)ANR 14 ACHN0024 CryoEM80S France
Czech Science FoundationGrant of Excellence in Basic Research (EXPRO2019) Czech Republic
CitationJournal: Cell Rep / Year: 2020
Title: Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts.
Authors: Anthony Bochler / Jailson Brito Querido / Terezie Prilepskaja / Heddy Soufari / Angelita Simonetti / Mayara Lucia Del Cistia / Lauriane Kuhn / Aline Rimoldi Ribeiro / Leoš Shivaya Valášek / Yaser Hashem /
Abstract: Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNA and several eukaryotic initiation ...Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNA and several eukaryotic initiation factors (eIFs) to the small ribosomal subunit (40S). Compared to their mammalian hosts, trypanosomatids present significant structural differences in their 40S, suggesting substantial variability in translation initiation. Here, we determine the structure of the 43S PIC from Trypanosoma cruzi, the parasite causing Chagas disease. Our structure shows numerous specific features, such as the variant eIF3 structure and its unique interactions with the large rRNA expansion segments (ESs) 9, 7, and 6, and the association of a kinetoplastid-specific DDX60-like helicase. It also reveals the 40S-binding site of the eIF5 C-terminal domain and structures of key terminal tails of several conserved eIFs underlying their activities within the PIC. Our results are corroborated by glutathione S-transferase (GST) pull-down assays in both human and T. cruzi and mass spectrometry data.
History
DepositionOct 27, 2020-
Header (metadata) releaseApr 14, 2021-
Map releaseApr 14, 2021-
UpdateOct 16, 2024-
Current statusOct 16, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0193
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.0193
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7ase
  • Surface level: 0.0193
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_11893.png

Downloads & links

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Map

FileDownload / File: emd_11893.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.1 Å/pix.
x 400 pix.
= 440. Å		1.1 Å/pix.
x 400 pix.
= 440. Å		1.1 Å/pix.
x 400 pix.
= 440. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.1 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0193 / Movie #1: 0.0193
Minimum - Maximum-0.08100778 - 0.11816512
Average (Standard dev.)0.000023447185 (±0.0053780125)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 440.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.11.11.1
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z440.000440.000440.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.0810.1180.000

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Supplemental data

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Sample components

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Entire : 43S preinitiation complex from Trypanosoma cruzi with the helicas...

EntireName: 43S preinitiation complex from Trypanosoma cruzi with the helicase kDDX60
Components
  • Complex: 43S preinitiation complex from Trypanosoma cruzi with the helicase kDDX60
    • Protein or peptide: kDDX60
    • RNA: initiator tRNA-Met
    • RNA: 18S
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: Elongation initiation factor 2 alpha subunit, putative
    • Protein or peptide: 60S ribosomal protein L40
    • Protein or peptide: Translation initiation factor, putative
    • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
    • Protein or peptide: 40S ribosomal protein S16, putative
    • Protein or peptide: 40S ribosomal protein S18, putative
    • Protein or peptide: 40S ribosomal protein S23, putative
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein S5, putative
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S13, putative
    • Protein or peptide: 40S ribosomal protein S27, putative
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: 40S ribosomal protein S33
    • Protein or peptide: 40S ribosomal protein S14, putative
    • Protein or peptide: 40S ribosomal protein S11, putative
    • Protein or peptide: Putative 40S ribosomal protein S9
    • Protein or peptide: 40S ribosomal protein SA
    • Protein or peptide: 40S ribosomal protein S2, putative
    • Protein or peptide: Putative 40S ribosomal protein S21
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: RNA-binding protein, putative
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: Eukaryotic translation initiation factor 2 subunit, putative
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S17, putative
    • Protein or peptide: Ribosomal protein S20, putative
    • Protein or peptide: 40S ribosomal protein S3, putative
    • Protein or peptide: 40S ribosomal protein S10, putative
    • Protein or peptide: Ribosomal protein S19, putative
    • Protein or peptide: 40S ribosomal protein S15a, putative
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: Protein translation factor SUI1 homolog, putative
    • Protein or peptide: Putative eukaryotic translation initiation factor 1A
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit E
    • Protein or peptide: Eukaryotic translation initiation factor 5, putative
    • Protein or peptide: Ribosomal protein S29, putative
    • Protein or peptide: eL41
    • Protein or peptide: JAB_MPN domain-containing protein
    • Protein or peptide: CSN8_PSD8_EIF3K domain-containing protein
    • Protein or peptide: 40S ribosomal protein S15, putative
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit 8, putative
    • Protein or peptide: eIF3A
    • Protein or peptide: 40S ribosomal protein S3a-2
    • Protein or peptide: Eukaryotic translation initiation factor 3 (EIF-3) interacting protein, putative
    • Protein or peptide: eIF3H
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit 7-like protein, putative
    • Protein or peptide: 40S ribosomal protein S4
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

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Supramolecule #1: 43S preinitiation complex from Trypanosoma cruzi with the helicas...

SupramoleculeName: 43S preinitiation complex from Trypanosoma cruzi with the helicase kDDX60
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#52
Source (natural)Organism: Trypanosoma cruzi (eukaryote)

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Macromolecule #1: kDDX60

MacromoleculeName: kDDX60 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 246.749875 KDa
SequenceString: MSSRYRELIA IFGEFLEEND FHVRISMDRV GEVKTLFSAK TIPVESETEA AIEVVTIEAA RRRDALRRTC NAVLRTRHSD QHVDVGTIV ADEEERLAWK FLIGALAAKN SEILPVVEAE LLLAAVEYHY ATARPELCDM FVHLSASEMF FIDGDSLFMA A LSPQSVDW ...String:
MSSRYRELIA IFGEFLEEND FHVRISMDRV GEVKTLFSAK TIPVESETEA AIEVVTIEAA RRRDALRRTC NAVLRTRHSD QHVDVGTIV ADEEERLAWK FLIGALAAKN SEILPVVEAE LLLAAVEYHY ATARPELCDM FVHLSASEMF FIDGDSLFMA A LSPQSVDW DLIQPLHVIY NAQKLLYDMH CRGARFHVVF FDSLLWIWES APAKLFMREN LRKTLMSLSE NDAKVGLSIS NF SSYYSEA FETYVKQWEP EFILMSDGEQ LGRLNPLQAF FVRPSAANNT HDSRRKANYT DADEANPYRL RRQYRSIVED EAV GDKAAL YYRCIHLWAA TRRLKVAYSS RIIYKENAMV VFTVRVDGAS FERAVTIESD VQALAQSMEH EVQLPAISSA SLGL LDEED LSCRERVVYA ALHAYLRASA RSEQEHQLCQ ALAITTYITG YLNKEARAQQ VRPNPILAGF LNEISPFLLA VWRHA DCTN GNGQEFDLID GHLFSAVSQQ LRTASVSELF DEYGVEDIES TWGVLDDQGS ADIVNAPLSY LPVIDSVDVE KLQPYP LIT HELVERLAKG FGISTHRADA PYPTDFGAAN ELAGWDITTP FDRLNDVIDA EGDAIAKSMM TEKEAKNVQE YYKKFVR NA LKQAQSMGIS GFAAHELAMV CSDNDSDDDA GGNSANNKTA GAKKVNKEHA GQRNKKERSK EDEIRERSNV IAATATVA E WHKQMNHLLH AVDMSHGRTT NRDRDESINT IMAAIKRLSQ EKFGKNFDPG YTLGGSTNTA VPLKLEMWRL LVAASQLRE VEFAFAMEDP ALKDSKGASK KKDSKSEYKM LYGFHVINQF VEREAVKGNH WGQLDPLRKA KPDMTIVEAR SYLRWVYLSF VEMHIQLKL KCRVVKLQLE NWRAERERAR LAQESPKIAL GIPLFLYCHH HVLAVIRDEG PRMSSEDIDT VRSALKHFDL P DSYYNKLD QCIARWQNMT LGTLLPSLLP QDKQLFETPE MLQLIHMGHL LERPFVREHD YRVAFNPDNW QRELLDIVDG RG SAVVCAP TSAGKTFISY YCMYKALRRT NKKVVVYLAP ARALINQAVA DVCARYGSKK YKNPGRYIYG ALGGADYHQF HDS CQVLLT VPETFETMLL SPKYTDWVEL IDYVILDEIH SMESNGNGDV WERILALLPC PFVALSATLG ETQQLCSWLN RVQG RLKEQ TEEMSGKMRD FEVHLLPSEG KSIQRWNDIK KYIYLPPPGA ALTQKKIKAQ YNNCYIRDLH PLSILTADQL QRGFP PDIS LVPSEVVSLF EKMHSKFNEV VWPNYSSLQL AKTLRAQLML IEPSKYFEAE TYITQERARQ YEAEVKNAFA YWAYLG HEG CELPENLVEE DLDDFSASMN MAVESILRTF AQKLNEDEAL LERHAADGME KKKRMLLRQQ HLQLLQQQEQ ENEPNQE ES MEQKSEEQGG AQEEEQEKET VGSVSFPGSR QFIREHILNV LRELIARDMG PTIVFSFESE DCGDLVKYVV EQLEEAES R YRKTNEFALY KARIERAAAA QEARRKQRES TLKQKRLTTG DDGDVEVADR DMSDGEGEDE LFVVPDVLPE FTFIGEKCT VEPEVVDSLM EDCEKEGEDL LLRALQRGIG MHHAGVKGKL RAHVERLFRG RHCGVIFSTE TLALGIHSPC RSVVLAGDHI LLNPTQFRQ MMGRAGRRGL DYLGHLVFLG ITMRRIKRLM TSSMTVIKGN VQMDPISNLR LLQLYDFNTL RHLKNEAGWK T HVLKLAER LFVNPLFFQG RNSVAGGNME GFTVEWLQML LGYFQREGLH FSDHASSLGS ILQDAMYVFR EAHVGNEGFS FI RMLTSGV FDKAHYSPLY DKKLNSGVLD EPLAELLAYL FSTHQTCGVP LEMHRSALLD PAVSTLWEGK TGPTQHRAVL SPI DVCSPT IHAFDNTDFF ALLSAFYNYL ASHLAPQTGA ALRLPCMKST NKKCRIFGGG STEFLLKQKL QESSVPYKAR SPFV AISGC GDLFTSVDDV TFTLRDGLYC DRTLLPILDL ADGWRHDGAQ ILINACLLDF LRAKAQIDTT RKNYRFTLLE ELNGL SQSL SYAVLNRAEK ILSNLAGLVR PTKLPRAKVL TAIMPDESEE GIFMAGAPRL LEVAERLNSL QPQIQKRLAE ELLTAK WAK RISEMNAQRK D

UniProtKB: ATP-dependent DEAD/H RNA helicase

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Macromolecule #4: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 15.706745 KDa
SequenceString:
MVFQKKKAEV TVRTSQFKVN KLLNRKQFIV EVNHPGWCGT VPAKLIRSRL ASLYKVADEN QISVFGFKTK FGGGKTTGFG LIYDDLAAL KRIEPNYRKA RLGMGKKKQP ARKSVKERRN RNKKIRGKAK GKMQTKKK

UniProtKB: 40S ribosomal protein S24

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Macromolecule #5: Elongation initiation factor 2 alpha subunit, putative

MacromoleculeName: Elongation initiation factor 2 alpha subunit, putative
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 47.700223 KDa
SequenceString: MASHGPVKDI ERVDYSTKCC CRTTDEAYYQ APKQYFVRHP TLSRRKLTLT EPFNVPFDAS TFEKVLIELE KASILSAAAA VTVDTEQSG GDNTQKPQWM KDLDKRQQRF LAACLGVTTW DGRDVCFYEE KLPKENDVVW VKVIQVNDTS AVVQLLEYGN H EGIIPYTE ...String:
MASHGPVKDI ERVDYSTKCC CRTTDEAYYQ APKQYFVRHP TLSRRKLTLT EPFNVPFDAS TFEKVLIELE KASILSAAAA VTVDTEQSG GDNTQKPQWM KDLDKRQQRF LAACLGVTTW DGRDVCFYEE KLPKENDVVW VKVIQVNDTS AVVQLLEYGN H EGIIPYTE ITRIRIRAIG KVIKVGRNEA AQVIRIDKEK GYIDLSKKQV TLKEAKECEA RFLKGNEVRS IVCHVADECG IP AAQAMEM IAYPLYRRQP GKHAWDWLHE LNRNRDVEGI LGPLHLPEKA QKLLLATLEH TVRNDTATIH ADIEMTCFQC DGV NALRDV LLLGRRFKAD QEPQIPISVT IVGPPRYRLR AKTEEREEGM RRMRETIETM AIEIAKRGGI LRVVHGPYAL GEEE QLKDE PNDDQDDDDD ETD

UniProtKB: Elongation initiation factor 2 alpha subunit, putative

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Macromolecule #6: 60S ribosomal protein L40

MacromoleculeName: 60S ribosomal protein L40 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 16.624496 KDa
SequenceString:
MQIFVKSAAG KTSAIQASAQ ETVASLKLKA GVCDGTLFYG GQCLCDEATL ADYGLQREST IQVMLPVEGG KGKKKKKKVF TKPKKPIHR HKLEKMRALK YFKVTENDDG SFKVERTRDE CPNPVCGAGV FMAQHKDRKY CGKCHLTYAA K

UniProtKB: Ubiquitin-ribosomal protein eL40 fusion protein

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Macromolecule #7: Translation initiation factor, putative

MacromoleculeName: Translation initiation factor, putative / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 38.227219 KDa
SequenceString: MAEGAKVARI VRRRRAEGDD DDDENERVPQ VEVEQQKKKT SRPKAPKAME AAPDATLATH SDPVTMGAGA SSSLPPPSAS TATVAPTAM TMGDRLDAPQ VAGSVEDVFE IAYDNYTSND LYALLKRITR QEVERRMIEH EQLRQKQQRV PQVERFLDAT S KTGSSGSS ...String:
MAEGAKVARI VRRRRAEGDD DDDENERVPQ VEVEQQKKKT SRPKAPKAME AAPDATLATH SDPVTMGAGA SSSLPPPSAS TATVAPTAM TMGDRLDAPQ VAGSVEDVFE IAYDNYTSND LYALLKRITR QEVERRMIEH EQLRQKQQRV PQVERFLDAT S KTGSSGSS GGGGNKATEE GCGYNYSVML RRLFFALNRN KESSAMTERN RLPEPKLEKI GKKKVIITNF GRICQAFNRP IQ DVKDYIE KELSVGGNLD SNDALILKYE IQKSTAFDNI LYKYLDEFVK CNSCHKIDTT LTKEGRRMEL RCNWCTATRT VQA VGSATY SAQVGKRSKA RLQAMTL

UniProtKB: Translation initiation factor, putative

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Macromolecule #8: Guanine nucleotide-binding protein subunit beta-like protein

MacromoleculeName: Guanine nucleotide-binding protein subunit beta-like protein
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 35.070312 KDa
SequenceString: MAVVYEGQLK GHRGWVTALA CPQITETYIK AVSTSRDNTL IAWGSNMDRN SEECEYGFPE RRLEGHSAFV SDVALSNNGD FAVSASWDH SLRLWNLQTG VCQHKFLGHT KDVLSVTFSP DNRQIVSGGR DNALRVWNVK GECLHTLGRG AHTDWVSCVR F SPSLETPL ...String:
MAVVYEGQLK GHRGWVTALA CPQITETYIK AVSTSRDNTL IAWGSNMDRN SEECEYGFPE RRLEGHSAFV SDVALSNNGD FAVSASWDH SLRLWNLQTG VCQHKFLGHT KDVLSVTFSP DNRQIVSGGR DNALRVWNVK GECLHTLGRG AHTDWVSCVR F SPSLETPL IVSGGWDNLV KVWDIASGRL LTDLKGHTNY ITSVTVSPDG SLCASSDKDG VARLWDLTKG EALSEMAAGA PI NQICFSP NRYWMCAATE KGIRIFDLEN KDVIVELAPE AQQKSKKTPE CMSIAWSADG NTLYSGYTDN VIRVWSVSEH A

UniProtKB: Activated protein kinase C receptor, putative

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Macromolecule #9: 40S ribosomal protein S16, putative

MacromoleculeName: 40S ribosomal protein S16, putative / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 16.841643 KDa
SequenceString:
MTAEKSHTHK QVQTFGKKKT AIAVATVTKA AQCNIRVNGV PVSQILPETL RAKIMEAVNV VGSRHFARLR IDVTVRGAGQ VAQAYATRQ AIAKGLVAYY QKYKNEVEKA ALKDKYLAYD KYLLIADPRR CEPKKWGRHS ARTRFTKSYR

UniProtKB: 40S ribosomal protein S16, putative

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Macromolecule #10: 40S ribosomal protein S18, putative

MacromoleculeName: 40S ribosomal protein S18, putative / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 17.585469 KDa
SequenceString:
MSLTLQSEQF QHIVRLLNTN VEGKRKVPFA LRMVKGVGIR FAYMVCKKAG IDVERRAGTL TAEELERISE VIADPAKFKI PDWFLNRQR DPKTGKTEHL TSAMVDTRLR EDLERLRKNR VHRGVRHAYG LRVRGQHTCT SGRHGKTVGV SRTK

UniProtKB: 40S ribosomal protein S18, putative

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Macromolecule #11: 40S ribosomal protein S23, putative

MacromoleculeName: 40S ribosomal protein S23, putative / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 15.960672 KDa
SequenceString:
MTKANGQHAA RKLVRLRRKN RWADKGWKKS HTFVAQKANP FGGSSHAKGI VLEKIGVGAK QPNSAIRKCV RVQLIKNDKK IIAFVPNDG CLHFIEENDE VLVSGFGRSG HAVGDIPGVR FKIVKVSNVG LYALYRQKKE KPRN

UniProtKB: 40S ribosomal protein S23, putative

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Macromolecule #12: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 25.246783 KDa
SequenceString: MGIVRSRLHK RKITGGKTKI HRKRMKAELG RLPAHTKLGA RRVSPVRARG GNFKLRGLRL DTGNFAWSTE AIAQRARILD VVYNATSNE LVRTKTLVKN CIVVVDAAPF KLWYAKHYGI DLDAAKSKKT LQSTTEKKKS KKTSHAMTEK YDVKKASDEL K RKWMLRRE ...String:
MGIVRSRLHK RKITGGKTKI HRKRMKAELG RLPAHTKLGA RRVSPVRARG GNFKLRGLRL DTGNFAWSTE AIAQRARILD VVYNATSNE LVRTKTLVKN CIVVVDAAPF KLWYAKHYGI DLDAAKSKKT LQSTTEKKKS KKTSHAMTEK YDVKKASDEL K RKWMLRRE NHKIEKAVAD QLKEGRLLAR ITSRPGQTAR ADGALLEGAE LQFYLKKLEK KKR

UniProtKB: 40S ribosomal protein S8

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Macromolecule #13: 40S ribosomal protein S5, putative

MacromoleculeName: 40S ribosomal protein S5, putative / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 21.292715 KDa
SequenceString:
MSAKAPKLFN KWSYENLQTT EIALSDHISK TATYVPHSAG RWQKKRFRKA RIPIVERLTN GLMFKGRGNG KKLQAVRLVK HTLEIIHLL TDQNPLQVVI DAVSKGAPRE DSTRVGSGGV VRRQAVDVSP MRRVNEAIYL MCKGAREAAF RNLKTLPECL A DEIVNASK GSSNSYAIKK KDEVERVAKA NR

UniProtKB: 40S ribosomal protein S5, putative

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Macromolecule #14: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 24.896504 KDa
SequenceString: MAATRKGGRM SVKPHLRKLR KLKRPNPSED EDSVAKALFE LEATHKTLRT QLPRFHINTV RTLSSPRFRR TATVVFYPLR FLMLVRKIQ RTLTAELEKR FPGSIVVLVA QRKITKRPKD VYKLQKVQRS RTSVAVFENI LNDLIYPCDV MGRRWRCRTD G TKVMKVFL ...String:
MAATRKGGRM SVKPHLRKLR KLKRPNPSED EDSVAKALFE LEATHKTLRT QLPRFHINTV RTLSSPRFRR TATVVFYPLR FLMLVRKIQ RTLTAELEKR FPGSIVVLVA QRKITKRPKD VYKLQKVQRS RTSVAVFENI LNDLIYPCDV MGRRWRCRTD G TKVMKVFL DSRDRKRVES RLPIIAHVYK QLTHRTVSFG FMWNPKLQQV SSR

UniProtKB: 40S ribosomal protein S7

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Macromolecule #15: 40S ribosomal protein S13, putative

MacromoleculeName: 40S ribosomal protein S13, putative / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 17.295621 KDa
SequenceString:
MVRMQGVGHG KSASSLPFCR TPPSWLKIAS RDVIDAVCKL AKKGLTPSRI GMQLRDSMGI AQVKNVTGRK ILRILKHKGM APEIPEDLY CLIKRATEMR KHLERNTKDR DTKFRLILVE SRIHRLARYY KRVKQLPPTW KYESSTASAM VA

UniProtKB: 40S ribosomal protein S13, putative

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Macromolecule #16: 40S ribosomal protein S27, putative

MacromoleculeName: 40S ribosomal protein S27, putative / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 9.581131 KDa
SequenceString:
MGFFDSDLSY PTVRSERMKH KRRRLVQGPN SYFMDVKCPG CNAITIVYSH ATSEVQCNGC ATKLCRPTGG KAMLTTGCGF RKKADH

UniProtKB: Ribosomal protein S27, putative

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Macromolecule #17: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 12.793177 KDa
SequenceString:
MTTKRRNHGR AKPPCSRGRT RPVHCFNCGR LAPKDKAVGR FVVRRMLDQA SARDVAEASN VYGAGFPMPK LYMKQRFCIA CAIHSRTVR ARPTDMRKRR FTTKVPFRPT GAK

UniProtKB: 40S ribosomal protein S26

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Macromolecule #18: 40S ribosomal protein S33

MacromoleculeName: 40S ribosomal protein S33 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 10.023522 KDa
SequenceString:
MTEVAAAKKD KKAAQEEVAV QQSDQAQVGL IIKVLGRTGS RGNVTQVRVR LMAEEGSPDA NRTIVRNVKG PCKEGDMLSL METEREARR LR

UniProtKB: 40S ribosomal protein S33

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Macromolecule #19: 40S ribosomal protein S14, putative

MacromoleculeName: 40S ribosomal protein S14, putative / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 15.596004 KDa
SequenceString:
MSKKQEVKFY GSSAAKDELV YGVVHIYASF NDTFVHVTDM SGRETFCKIT GGMKVKADRD ESSPYAAMMA AQDVVARCKE CGINALHVK MRATGGVGTR SPGPGAQAAL RALARAGMKI GRIEDVTPIP TDSTRRKGSR RGRRL

UniProtKB: 40S ribosomal protein S14, putative

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Macromolecule #20: 40S ribosomal protein S11, putative

MacromoleculeName: 40S ribosomal protein S11, putative / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 20.018307 KDa
SequenceString:
MTAPEQYARV HSVDLTLQHE KAYQRQTAVN ENSHNPNKKH VNMSGHIRYS KKIGLGFKTP KSAINGKYID RKCPFTSDVS IRGRILRGV VHSTKMKRSI VIRRDYLHFI RKYQRYQKRH RSLTVHCSPC FDPKPGDEVI VGQCRPLSKT IRYNVLEVVS K SAPGKLGR KFSKN

UniProtKB: 40S ribosomal protein S11, putative

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Macromolecule #21: Putative 40S ribosomal protein S9

MacromoleculeName: Putative 40S ribosomal protein S9 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 22.180945 KDa
SequenceString:
MRNYNNFNRV WKAPRRPFEK ERLDREMKLC GQYGLRCKRE IWRVNMTLSK MRRTARLLLT LPENHPRRQL EGSAIMRRCH DYGFLEEEK DKLDYVLSLT VPDILERRLQ TVVFKHGLAK SVHHARVLIL QRHIAVAKQI VTIPSFIVRV SSEHHIAFAD A SPFGNGRP GRVKRVKRKA AKKGAAGGDN DE

UniProtKB: Putative 40S ribosomal protein S9

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Macromolecule #22: 40S ribosomal protein SA

MacromoleculeName: 40S ribosomal protein SA / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 27.913182 KDa
SequenceString: MTSVESGAKV LRMKEGDVQK LVAMHCHLGT KNRSNAMKKY IHSRTKEGTN IIDLHMTWEK LILAARVIAA VENPQDVTVC STRLFGQRA IFKFSQLVGT SFLAGRFIPG TFTNQIQKKF MQPRVLLVTD PRTDHQALRE ASLVNIPVIA FCDTDAPLEF V DIAIPCNN ...String:
MTSVESGAKV LRMKEGDVQK LVAMHCHLGT KNRSNAMKKY IHSRTKEGTN IIDLHMTWEK LILAARVIAA VENPQDVTVC STRLFGQRA IFKFSQLVGT SFLAGRFIPG TFTNQIQKKF MQPRVLLVTD PRTDHQALRE ASLVNIPVIA FCDTDAPLEF V DIAIPCNN RGRYSISMMY WLLAREVLRL RGTIPRSVPW DVKVDLFFYR DPEEALKHEE VNQAAAPVAE VDEGFGWVER DN NAWEQ

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #23: 40S ribosomal protein S2, putative

MacromoleculeName: 40S ribosomal protein S2, putative / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 28.574951 KDa
SequenceString: MADAQRAPSG EQRGFGRGRG GRGGRGGRGG RGGSNEKEEW VPCTKLGRLV KEQKITSLEE IFLFSMPIKE HQIVDQLIKE SDMRDELML IVPVQKATSA GQRTRFKAFN IVGDGNGHIG VGARVGKEVS LAIRASMIAA KLNVVPIRRG YWGNKIGEPH T IPMKVTGK ...String:
MADAQRAPSG EQRGFGRGRG GRGGRGGRGG RGGSNEKEEW VPCTKLGRLV KEQKITSLEE IFLFSMPIKE HQIVDQLIKE SDMRDELML IVPVQKATSA GQRTRFKAFN IVGDGNGHIG VGARVGKEVS LAIRASMIAA KLNVVPIRRG YWGNKIGEPH T IPMKVTGK CGSVSVRLIP APRGAGIVAA PVPKKILEFA GVEDVYTSSC GNTRTRGNFI MATFYALRKT YGFLTPDLWA ET EVSRDPT DEHSDFLTMG SKMIAA

UniProtKB: 40S ribosomal protein S2, putative

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Macromolecule #24: Putative 40S ribosomal protein S21

MacromoleculeName: Putative 40S ribosomal protein S21 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 27.634291 KDa
SequenceString: MTTIGTYNEE GVNVDLYIPR KCHATNNLIT SYDHSAVQIA IANVDANGVL NGTTTTFCIA GYLRRQAESD HAINHLAISK GIIRIKTGK KPRAKKLKNV KGLGVRGLPR GALQQRGARA LPTQRGIAQR GGAQKGNVRK LQPQQQKQRS QLNQRSQQQK Q RPQQQHGA ...String:
MTTIGTYNEE GVNVDLYIPR KCHATNNLIT SYDHSAVQIA IANVDANGVL NGTTTTFCIA GYLRRQAESD HAINHLAISK GIIRIKTGK KPRAKKLKNV KGLGVRGLPR GALQQRGARA LPTQRGIAQR GGAQKGNVRK LQPQQQKQRS QLNQRSQQQK Q RPQQQHGA RPTRKEEGGR MQRGVRDAPQ ARKQQGRDAP QARKQQGRNE PQARKQQGRN EPQARKQQGR NEPQARKQQG RN APRSQKA

UniProtKB: Putative 40S ribosomal protein S21

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Macromolecule #25: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 12.379955 KDa
SequenceString:
MPPKKNAKPT KPAPKKGKME NMNKGAKKAA KKWSKGRTRE ALQNAVVFDK ETMDKLLSEV PKYKVITPSI ISDRLKISVA LAAKGLQHL CRQKLIKLVS CSSKFRLYSR V

UniProtKB: 40S ribosomal protein S25

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Macromolecule #26: RNA-binding protein, putative

MacromoleculeName: RNA-binding protein, putative / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 27.676303 KDa
SequenceString: MPAKSANKPA SKPASKPAAK PAAKPAAKAP APKAEKKGAA KAPAPKAAAP APKAAAAAPK PAVRDAKQRS DAANHNGLYV KNWGQGSVD DARALFGTAG KVVGVRVRRR RYAIIFFENA AAVKKAIDLF NGKEFMGNVL SVVPAKTTPK PDPHANSSVV F VSPIFRAS ...String:
MPAKSANKPA SKPASKPAAK PAAKPAAKAP APKAEKKGAA KAPAPKAAAP APKAAAAAPK PAVRDAKQRS DAANHNGLYV KNWGQGSVD DARALFGTAG KVVGVRVRRR RYAIIFFENA AAVKKAIDLF NGKEFMGNVL SVVPAKTTPK PDPHANSSVV F VSPIFRAS TTKKQILELF SGMKVLRLRT YRNNYAYVYL DTPAAAQRAV KEKNGAEFRG KQLRVALSTR SLAKDRARAE RA RLLMAAQ KFNKRKNHTK

UniProtKB: RNA-binding protein, putative

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Macromolecule #27: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 15.79211 KDa
SequenceString:
MAEETPVLEV SPAVVDAVMD ATPENLEDAL RIVLQKSREV NGLIRGLSEV ARALDRRTAH LCVLAEDCED EEYKKLITAL AKQGEVDLI NVEEREKLAQ WAGLTKMDST GELKKTFKCS CVAVRDFGER TRALEFLLSH LR

UniProtKB: 40S ribosomal protein S12

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Macromolecule #28: Eukaryotic translation initiation factor 2 subunit, putative

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit, putative
type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 52.642418 KDa
SequenceString: MDENDIADFT MDENTFVTNE QNERGLATQD INALNLDKIN AETFEVMSRQ ATVNIGTIGH VAHGKSTVVK ALSGVKTQKY HREAVMNIT IHLGYANAKV FKCDKCELPA AFHAFPSSQP DKTDCPTCGS PLTLKRHFSF VDCPGHDVLM ATMLNGAAIM D AALLLIAA ...String:
MDENDIADFT MDENTFVTNE QNERGLATQD INALNLDKIN AETFEVMSRQ ATVNIGTIGH VAHGKSTVVK ALSGVKTQKY HREAVMNIT IHLGYANAKV FKCDKCELPA AFHAFPSSQP DKTDCPTCGS PLTLKRHFSF VDCPGHDVLM ATMLNGAAIM D AALLLIAA NEPFPQPQTL EHLKAVEIMR LANLVILQNK IDLVGEVHAQ DQYHKIRNYI DSTIGSNIPI IPISAQLKRN ID YLLEYLC HIPLPTRQLN CPAHMTVVRS FDINKPGEVD IENLRGGVAG GTVTRGIIRV NQVLEIRPGQ VHAQTGGTFS CTP LRTRAL TLKAEDNSLQ YAVPGGLIAV GTTLDPTLTR QDKMVGHMIA DEGSLPEVYA EIEVQYFLFE EMVGRSKQRD RNAK RVQKL NLQETLQINV GTLTAGATVV NITKNPDIAK LTLVTPVCCT LDEHIAISRL VEKNFRLIGW GIIRRGVPVK LN

UniProtKB: protein-synthesizing GTPase

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Macromolecule #29: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 28.344391 KDa
SequenceString: MKLNIAYPRN GTVKQFEITE EVLRRVSLTD YRLGNEVDGA IFGDAFRGYT FKLQGGSDKQ GFPMVQGVMA PSRVSLLVKR GAVGFNTFR GYQGERRRKS LRGCILASDI AVLNVVIVKT GEQPIEGVTD VSVPRRLGPK RANKIRKLFN LSRGDDVRKY V IRRKVVKE ...String:
MKLNIAYPRN GTVKQFEITE EVLRRVSLTD YRLGNEVDGA IFGDAFRGYT FKLQGGSDKQ GFPMVQGVMA PSRVSLLVKR GAVGFNTFR GYQGERRRKS LRGCILASDI AVLNVVIVKT GEQPIEGVTD VSVPRRLGPK RANKIRKLFN LSRGDDVRKY V IRRKVVKE GKKDRFKAPK IQRLITTAVK ARRAKKAKVA IEKVRKSAEE RRAYLHLIGT RRRAARQRNS ARRHAHKVAA QK QALALFK GKK

UniProtKB: 40S ribosomal protein S6

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Macromolecule #30: 40S ribosomal protein S17, putative

MacromoleculeName: 40S ribosomal protein S17, putative / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 16.255222 KDa
SequenceString:
MGKIRTKTVK RASKQLVEKY FSKLNKDFYQ NKRVVMDVTI ARSKKLKNKI AGYTTHIMKR LARGPVRGIS LKLQEEERER RMDYVPEVS HVDQAIQDGV HVDKQTFSML KRMETGVPRH VALTTVVAPV TKKGGRRAPA RK

UniProtKB: 40S ribosomal protein S17, putative

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Macromolecule #31: Ribosomal protein S20, putative

MacromoleculeName: Ribosomal protein S20, putative / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 13.248478 KDa
SequenceString:
MAYVKKDQAP AQETQRVLVK MTSRNAKAVE NVMTELLLHA RNEKVVLRGP VRLPTRTLKV TTRKTPCGNG TNTWDTYELK IYKRLVELR APTELVKKIT SFPIDAGVDV AITIPHDQ

UniProtKB: Ribosomal protein S20, putative

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Macromolecule #32: 40S ribosomal protein S3, putative

MacromoleculeName: 40S ribosomal protein S3, putative / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 24.188436 KDa
SequenceString: MGPLSKKRMI IRDGVFYAEL FEFLKRELAD DGFAGVEHRV TSTRTEIVIR ATKTREVLGE KGRRIRELTA CLQQRFHYKE GKLQLFAER VEVRGLSAMA QAESLRFKLL SNLQVRRAAM GIIRHVMESG AKGCEVTVGG KIKGQRAKSM TFRDGYMIKS G TAHKNFVD ...String:
MGPLSKKRMI IRDGVFYAEL FEFLKRELAD DGFAGVEHRV TSTRTEIVIR ATKTREVLGE KGRRIRELTA CLQQRFHYKE GKLQLFAER VEVRGLSAMA QAESLRFKLL SNLQVRRAAM GIIRHVMESG AKGCEVTVGG KIKGQRAKSM TFRDGYMIKS G TAHKNFVD VATRHCHLRA GTLGVKVKIM LPKSMQNEEE ILPDVITVLE PKEITA

UniProtKB: 40S ribosomal protein S3

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Macromolecule #33: 40S ribosomal protein S10, putative

MacromoleculeName: 40S ribosomal protein S10, putative / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 18.284725 KDa
SequenceString:
MATYVPKRSR DDVYRFFFTE GVISCKKDPL GTWTGSLGGK TFTVPSIQVM QLMRSMKSRG LIKEQFAWRH FYWFLNDEGV EYLRKYLFL PHDAVPNTHK AEYKVLEREG GRGRGRGEGR GRGRGEGRGR GRGEGRGRGR GFHSERDAYR TGAAAAPAVE A Q

UniProtKB: 40S ribosomal protein S10, putative

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Macromolecule #34: Ribosomal protein S19, putative

MacromoleculeName: Ribosomal protein S19, putative / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 18.861287 KDa
SequenceString:
MVAARTKANS KIRRIGKKKG ATMKDVHPWR WVKLCSQHFK QEGKLMVPNC TEIVKSSHGR ERAPQNPDWY YIRCAAILRA VYMRPGVGY GGLSKRFSNK KNRGSRPEIT VRASKGLLHW GCKSLTKLGL IEKCESSGHR ITKNGRKLAD AIAFKVAIRK L NTATSKN

UniProtKB: Ribosomal protein S19, putative

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Macromolecule #35: 40S ribosomal protein S15a, putative

MacromoleculeName: 40S ribosomal protein S15a, putative / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 16.255128 KDa
SequenceString:
MLPTASQGTK PAKEKMTMMS VLANALRCIA SAERRGKRQV LIRPSSKVVV KFLQVMQKHG YIGEFEIIDD HRAGKIVVNL NGRLNKCGA ICPRFDCTTP DFEKWVKNIL PSRQFGFVVL TTSLGIMDHE EARARNTGGK VLGFFY

UniProtKB: 40S ribosomal protein S15a, putative

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Macromolecule #36: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 7.445938 KDa
SequenceString:
MGKVHGSLAR AGKVKNQTPK VAKQEKKKQP RGRAFKRLKY TQRYLAKTLK PGEKLHMNKQ PPGKAG

UniProtKB: 40S ribosomal protein S30

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Macromolecule #37: Protein translation factor SUI1 homolog, putative

MacromoleculeName: Protein translation factor SUI1 homolog, putative / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 12.577262 KDa
SequenceString:
MLNNELANLV DQQKRSVQDA LEAQKVHIRV QQRKGKKFVT SVQGLNQALN FRRISREFQR RWGCNGTVIV TPDAGTVIQL QGNWSEEIK KFLLDENMAT EQNLEIHSLN

UniProtKB: Protein translation factor SUI1 homolog, putative

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Macromolecule #38: Putative eukaryotic translation initiation factor 1A

MacromoleculeName: Putative eukaryotic translation initiation factor 1A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 18.418516 KDa
SequenceString:
MGKGGKSFKA GNAKGIMQNQ KRDIVYADPQ EGEEYAQVKK ALGNLRLELQ LADGSKAIGS IRGAMVRKVW IGQGDVVLVS KRSFNKNDI VDVIHRYNPA EVRALVKDEV IPRDFRSSDE RDAKNAHSDY VFVAENDDDG GADDDDQNVL DRNKVVLDDP L ATFDEL

UniProtKB: Putative eukaryotic translation initiation factor 1A

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Macromolecule #39: Eukaryotic translation initiation factor 3 subunit E

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit E / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 46.320785 KDa
SequenceString: MTEMLRCLLP HLDKHLALGL LYFYDEQGLD VADALRAVQA TTALTAEGEV SVEQERKIKE TAQRARPALD EFFEKSETEN STYQFKLTG SEIDALRSNR ELSRDVLEAK GITSNVMKAV MELAYLYYDA ARYTDASELL SLCQCVVGYE IDQRTLLWGK L VSDMCTCN ...String:
MTEMLRCLLP HLDKHLALGL LYFYDEQGLD VADALRAVQA TTALTAEGEV SVEQERKIKE TAQRARPALD EFFEKSETEN STYQFKLTG SEIDALRSNR ELSRDVLEAK GITSNVMKAV MELAYLYYDA ARYTDASELL SLCQCVVGYE IDQRTLLWGK L VSDMCTCN WPSAIAAAEK IRRQQNADVF EEDIFRVANT TTTRERAWLL HWVLFPFFKG GNQYSTHLLN FVFDIKTNFV YQ SVVETVC PHYLRYICAA AILNKQRRSA LRSAAAMVLN VYEYSDPITQ LVNAIVNRQS FEDALALLPE VKSTALGDYF LIL HANEIL ENARRLIFAR YMMTHGVVSI PYVAEKLGTR TADAEVWLAN LISETKQRAK IDSVSEQMIV GSQARSVHQT VLDK LEVVD RR

UniProtKB: Eukaryotic translation initiation factor 3 subunit E

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Macromolecule #40: Eukaryotic translation initiation factor 5, putative

MacromoleculeName: Eukaryotic translation initiation factor 5, putative / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 42.887738 KDa
SequenceString: MSVPMIPIDP DKRDDLYYRY KMPTIQTKVE GSGNGIKTVF PNIHDVCLAI NRPEEVLMKF FQSEIGAQRT VLEKDDKFLI MGSHTEERV QEKIYDFIRK FVLCRSCRNP ETQLFVERNK KDVPHISMSC GACGKVMKLS DLDARYVTAI VTYFAKNPQV A MKKGAGTA ...String:
MSVPMIPIDP DKRDDLYYRY KMPTIQTKVE GSGNGIKTVF PNIHDVCLAI NRPEEVLMKF FQSEIGAQRT VLEKDDKFLI MGSHTEERV QEKIYDFIRK FVLCRSCRNP ETQLFVERNK KDVPHISMSC GACGKVMKLS DLDARYVTAI VTYFAKNPQV A MKKGAGTA EARSSQKNQQ AAAAAAAAAV APPAEPEKKQ IQRSDLEDTR EPPQKILARC MQQYPDESEE VMRRCIELMT TY NLKEKMG PLLVLDGIEL AEKEFMAGLR RHSRLLKRFC RVTGTALLDA ATDEATKSDL LRREKRLQSA TIEESARICA RRF EPEQMV VILFVLFIEG VLKSATIEEW CGDEKPISKV DPAVDKKMKQ AAAPLVEWLV GST

UniProtKB: Eukaryotic translation initiation factor 5, putative

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Macromolecule #41: Ribosomal protein S29, putative

MacromoleculeName: Ribosomal protein S29, putative / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 6.651826 KDa
SequenceString:
MGHLEQWRSR QKIGMGKGSR HCVICSNQKA VIRKYELNVC RQCFRENAAN IGFSKLR

UniProtKB: Ribosomal protein S29, putative

+
Macromolecule #42: eL41

MacromoleculeName: eL41 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 4.348417 KDa
SequenceString:
MGTISRPGGM RPKWHKKRIK RLKRRRRRMR QRSK

UniProtKB: 60S ribosomal protein L41

+
Macromolecule #43: JAB_MPN domain-containing protein

MacromoleculeName: JAB_MPN domain-containing protein / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 38.589625 KDa
SequenceString: MAQMMGTLVL PPFVAAAIVE HAKRRRNSAG YLVGSRSGNQ ITVTDYIPCT HESTSDVRTR AYAEELKERV ALKKCYTPSI TLVGWYAAA TPEPGKERAF DLWCQAPGAS FSKIRSHNQA VMLLGRMPTA ADLSIRWEAY ITSNMNDGDS LQCERMQQLT V CVEAETPS ...String:
MAQMMGTLVL PPFVAAAIVE HAKRRRNSAG YLVGSRSGNQ ITVTDYIPCT HESTSDVRTR AYAEELKERV ALKKCYTPSI TLVGWYAAA TPEPGKERAF DLWCQAPGAS FSKIRSHNQA VMLLGRMPTA ADLSIRWEAY ITSNMNDGDS LQCERMQQLT V CVEAETPS MNVLLAEMIS KTLYNGSMPY PTNRITNLDR VAVEAESREA EFGRKDNSRN DAEPRPVEAA LLNVQNKLHQ AI SHARAIL VSGNKNKSER QNESAAVVEN YEAILAEKSQ QSSRDDFITE SYKDALMIKY TAALLRRHVM EIERHGRQEL KEK TQHGHG HGQNNTSSPR GPRKLGAFR

UniProtKB: JAB1/MPN/MOV34 metalloenzyme domain-containing protein

+
Macromolecule #44: CSN8_PSD8_EIF3K domain-containing protein

MacromoleculeName: CSN8_PSD8_EIF3K domain-containing protein / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 22.458547 KDa
SequenceString: MSRDLAKEVD EALKQDKYDI QLFLRFLKSY VMAGTQPDKR LLLGILLQTL PRFHSNDFLA CISLVPGHVQ DAPYVEKELG TIYDLENYL SCGRFVQFWE VWNQSKSLPA ASPSFESQVR AGILIVVSST LEKVPVAKMA AYLGVNPDQL QSTLTEAASI A GEAVSIVS ...String:
MSRDLAKEVD EALKQDKYDI QLFLRFLKSY VMAGTQPDKR LLLGILLQTL PRFHSNDFLA CISLVPGHVQ DAPYVEKELG TIYDLENYL SCGRFVQFWE VWNQSKSLPA ASPSFESQVR AGILIVVSST LEKVPVAKMA AYLGVNPDQL QSTLTEAASI A GEAVSIVS CDTETVTFAK SIFNAPESDS NQQPLRFSDI VSIVS

UniProtKB: CSN8/PSMD8/EIF3K domain-containing protein

+
Macromolecule #45: 40S ribosomal protein S15, putative

MacromoleculeName: 40S ribosomal protein S15, putative / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 17.554453 KDa
SequenceString:
MTSNITQERY EQLKKERTFH KFTYRGLEID PLLALSEEEF KTLVHARARR HMNRHANRRA PVLLKRLREA KKDVKAGEKP RAVKTHLRD VVITPEMVGS VVGIYNGRQF NAVEIKGEMI GHYLGEFSMT YKPVIHGRPG VGATHSSRFI PLK

UniProtKB: 40S ribosomal protein S15, putative

+
Macromolecule #46: Eukaryotic translation initiation factor 3 subunit 8, putative

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit 8, putative
type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 82.331086 KDa
SequenceString: MSNFFDVSDS DESLDEVIHH DEQVERKVAQ IDPKWFEVTD DEDADERQVV LSRNEKSLNE IQTTCDLFDF NVDHESWSEA EKAFIQLRQ KASAHKEKFK VIPWPFLECL RNTPDLSEKM DEKETFKRPE DFYSLKRLIK ALQELTEIHK NDIERLHDEE S EEDGGDEG ...String:
MSNFFDVSDS DESLDEVIHH DEQVERKVAQ IDPKWFEVTD DEDADERQVV LSRNEKSLNE IQTTCDLFDF NVDHESWSEA EKAFIQLRQ KASAHKEKFK VIPWPFLECL RNTPDLSEKM DEKETFKRPE DFYSLKRLIK ALQELTEIHK NDIERLHDEE S EEDGGDEG QGEEEKELTE EDIAQELKQS VIQKGKRAAR CQKLAQESKK RGLTALRITA LGILAEALLE EDTRLPYVAT AT WTRSFDA VSRIYSLITE NPAIAVKEVF SGDLTSKRAV IMDGLCGLLQ KLHVHLQRIA QFKTGATDEY FEIIHLENQL VDL ADSVLG YYQQRKRGKA ICCQILIEIL GSRRQQAHDI LYHKMTRLTR NIVTTSVIET VRELYQELLV IGNEEAKCSA LLYL AYQMG LEGKYRDGRD LVLRSGVEET VEKSVHLAIL YNRVIAQLGL ASFAAGDVIQ AYNLLSSLWS NRNHDVLISQ RMPDY VKEN DEEELKFRDL LVPPHAYIQH AQLELATMLS TLVVDTPKEA KKPYEGSRHQ SYFFRIINQM AYQPLLGDPV EFREQL TAA YINLKLGDYA KASEVIKNMG AWSMMPNGDE ALKTFLQHLK EAALRIFCYN NRCNFATISV DLMMKKYGLN ENEVKCI IN DIISESNSSL IAFWDREDKY LHVDRSNTSR LQYLVEGIAE SVVEVAQYSE RRVRDSDFRG RGRGRGRGRG RGGF

UniProtKB: Eukaryotic translation initiation factor 3 subunit 8, putative

+
Macromolecule #47: eIF3A

MacromoleculeName: eIF3A / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 87.439898 KDa
SequenceString: MSTEDKTRGC LTKAQTLRAS GNYKEAVAAL QSLSEHGVQW GPMYIAALDL LAELCFSQEQ GITVDRFFPA FKWNRNKLRG SQHLEEGTK RIVEIAMKHL RALGERAHTN AKATGETPSE EELILAALSG VSPAQRAKER YLVPAETVAQ FLGSELLSFN A IGHSRKLL ...String:
MSTEDKTRGC LTKAQTLRAS GNYKEAVAAL QSLSEHGVQW GPMYIAALDL LAELCFSQEQ GITVDRFFPA FKWNRNKLRG SQHLEEGTK RIVEIAMKHL RALGERAHTN AKATGETPSE EELILAALSG VSPAQRAKER YLVPAETVAQ FLGSELLSFN A IGHSRKLL PIYLDTATEL IKYCQQHNLK RAIGRIADAY VRFFRRFLLS PIPSIVETDN PHLITMHKEL EADREDFYKE KP NTDRAVR VFCHLLQTLT EMNSWHAAWS TLQCFTRVMQ EITQHPDPSR ECQIIANSAM AAVFWKCSHY AFHAHCLGVA AFL TGNGGE AAAAASRAVL ATLCVPNTNK ERRNFERGSD SVFEKNARIA QLFGLQSAPA GLALWQRLQR MQVFQKAFPE VQAL DGLLR NEMSDENIAR QAIKQLSIIV QKDPSLEMYE KPLRKVVIQR YLECMAVRTT RVEASSLQIG ENEASEEVYI HEIEP YILN ESGIAVEIDH KTGFISFSNT TKMRVLEAFD ALAERVDFHP PALRRKLDIR PEHLLRAHDR SSIIHRLQHT CEETAE ARR QSAKEREEAE RENARLERIQ NEEKKKEAVR LAQEARGLAE YQEHINQNRR KVVLRRLKEK YKGFDAPPAL TLRASTD FV QELTTLLTAH LKKTTQQKTA DVTKMNHFER ACRELEIPKR KAIELEELEQ HKAERAAARE NFLIQHRKEF EKRQLDNQ I LKKFIKEAAA FAEQTQMKGK TSKRDEQQML LQQERERLQG L

UniProtKB: Eukaryotic translation initiation factor 3 subunit a

+
Macromolecule #48: 40S ribosomal protein S3a-2

MacromoleculeName: 40S ribosomal protein S3a-2 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 29.134721 KDa
SequenceString: RISKGGKRGK KKAQETMSRK EWYDVVAPKN FEVRQFGKTI CNKTQGTKIA ADYLRGRVYE SNLADLNKTQ GDDDAYRKVK FVVQEVQGR NLLTQFHSME MTSDRVYFLL RKWCTTIEAA VEAKTADGYT LRLFVIAFTK KQSNQLSKNC YAKTRLVKWV R HRITNLIR ...String:
RISKGGKRGK KKAQETMSRK EWYDVVAPKN FEVRQFGKTI CNKTQGTKIA ADYLRGRVYE SNLADLNKTQ GDDDAYRKVK FVVQEVQGR NLLTQFHSME MTSDRVYFLL RKWCTTIEAA VEAKTADGYT LRLFVIAFTK KQSNQLSKNC YAKTRLVKWV R HRITNLIR QRLSKVNINE AVTLLTRNIL RDRLAKRCNP IVPLRDLRIR KVKVVRTPRF DAQALLNAHG EIPASAEGEA RV VEEAQEA PAAEATA

UniProtKB: Small ribosomal subunit protein eS1B

+
Macromolecule #49: Eukaryotic translation initiation factor 3 (EIF-3) interacting pr...

MacromoleculeName: Eukaryotic translation initiation factor 3 (EIF-3) interacting protein, putative
type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 56.33125 KDa
SequenceString: MTTNPDIESD IPHEVYSFFR ALNKAVDSWD TSLLHELYEN QFPGLTQNFY MAGPGQFRPW PSLRIKAVAD CFKNRTAEQL YSFLRLKHL FTDRAVSPND ARSSWDTFNN LFNALPSGSC DVPNWLLWDI FDEFLFQMTV VYQKRFTGRV GWSVNEGMQL M ERVVAESG ...String:
MTTNPDIESD IPHEVYSFFR ALNKAVDSWD TSLLHELYEN QFPGLTQNFY MAGPGQFRPW PSLRIKAVAD CFKNRTAEQL YSFLRLKHL FTDRAVSPND ARSSWDTFNN LFNALPSGSC DVPNWLLWDI FDEFLFQMTV VYQKRFTGRV GWSVNEGMQL M ERVVAESG IEEAIQSENL DEITKPGARH AQWMCGFFGI VTIAKVNVLL GDYMGALSAL KPLDVYGRGR QILLVVAPAY VS LLYHMGF SYLMLRRYAD ASRVFRLSLT TKVSSRKFSE KMQFDCAYMH VISCILGGMQ PDNLSWLVEP RKLSGFEDEK ELL SAGDEE RFREVFDRCS PKFLAIPPIT TIMYKGTDGK ELQARLFRRA VKQQEDIIKL RGFFGVYQTT TTELVKTVLD VDDG HVPLF AMRLRSRQLV HDGSSADLLS GSYAVRSAID YTVKGENIDV VQKSSYRTTE SKYFMRINNL RRRQRHFENQ RQERR QAQP ISIAK

UniProtKB: Eukaryotic translation initiation factor 3 (eIF-3) interacting protein, putative

+
Macromolecule #50: eIF3H

MacromoleculeName: eIF3H / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 38.408816 KDa
SequenceString: MFHGFPDVQI APRDTVISEE FHLHDKEGRM GTLLQPMAQR MRISFSALTC LGSYAQNATC HYALFGRLCG VQLNETIEVT NVLPPVINP RPPEDETPEQ REKRLLAQQR EERQMYERMG KMFFKEELDS YHVGYFAICS AYTNAPYSVR TVQQLAQLAL D GNPSVLVV ...String:
MFHGFPDVQI APRDTVISEE FHLHDKEGRM GTLLQPMAQR MRISFSALTC LGSYAQNATC HYALFGRLCG VQLNETIEVT NVLPPVINP RPPEDETPEQ REKRLLAQQR EERQMYERMG KMFFKEELDS YHVGYFAICS AYTNAPYSVR TVQQLAQLAL D GNPSVLVV YDPFRTSLMG KLYLRAFVPT REYVEFYTRL TDKRNILREN RLMRECNVGK GGVLREVKVE VDVDEYQLLC LS GFNVAPL SSTCRTLHSE VMTDYMAALI ESVRHNADEL SRGLHSESYF SQKEESYGPL GQRIDTLLKL MQLREQTQHL ESL CDGVLL NTSLLRDL

UniProtKB: Eukaryotic translation initiation factor 3 subunit h

+
Macromolecule #51: Eukaryotic translation initiation factor 3 subunit 7-like protein...

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit 7-like protein, putative
type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 57.492965 KDa
SequenceString: MGFELPEIFV NAPFTWGPPP SEIEMDGMKV RLYQKTDAIA PSDWLEAMLD QANETKQFTT VKDENRLKAL RNLHAKERRH GPERRFVKH YQNARSHFAN KAKRNLTLLP DTVKVPTDVL IFAEFTQAEL AKMQNLQDAP TVTDISLHNR PLVYNNAMEK A SCKTPIRL ...String:
MGFELPEIFV NAPFTWGPPP SEIEMDGMKV RLYQKTDAIA PSDWLEAMLD QANETKQFTT VKDENRLKAL RNLHAKERRH GPERRFVKH YQNARSHFAN KAKRNLTLLP DTVKVPTDVL IFAEFTQAEL AKMQNLQDAP TVTDISLHNR PLVYNNAMEK A SCKTPIRL EETNKSEEFF ARSTTVEDGT LRDILKKEAA GTHPIVVTTD EVLALMMTCS RGLHPWHLEI FRYNRMVFIS KT EKSNVEV QWVGETADTL RRPVENDPNE SERITNLAKE STKAFNAFVA QACLKTRYQM KCEKNPFPDT QPRLYRYRRF VMH AETDDH YDIIVRCEID AVQNDKYVRI FGLLEQCADG VESEWRKTLD SQGAKWISDE YRRNAQKMSR WVCLCHLSGT LMKI GFLSR SYRSNGTLDP NKHEVLATHT KDPGPLAAQL GIKVGNMWAI ADAIIMAFLK QQDLSEALLV KKSGGQSIML IEKME DEEE EDDDDDDDDD GGSSDGDV

UniProtKB: Eukaryotic translation initiation factor 3 subunit 7-like protein, putative

+
Macromolecule #52: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 30.936521 KDa
SequenceString: MTKKHLKRLY APKDWMLSKL TGVFAPRPRA GPHKLRECMT LMIIIRNRLK YALNAAEAQM ILRQGLVCVD GKPRKDTKYP VGFMDVVEI PRTGDRFRIL YDVKGRFALV KVGEAEGNIK LLKVENVYTS TGRIPVAMTH DGHRIRYPDP RTHRGDTLVY N LKEKKVVD ...String:
MTKKHLKRLY APKDWMLSKL TGVFAPRPRA GPHKLRECMT LMIIIRNRLK YALNAAEAQM ILRQGLVCVD GKPRKDTKYP VGFMDVVEI PRTGDRFRIL YDVKGRFALV KVGEAEGNIK LLKVENVYTS TGRIPVAMTH DGHRIRYPDP RTHRGDTLVY N LKEKKVVD LIKSSNGKVV MVTGGANRGR IGEIMSIERH PGAFDIARLK DAAGHEFATR ASNIFVIGKD MQSVPVTLPK QQ GLRINVI QEREEKLIAA EARKNMQTRG VRKARK

UniProtKB: 40S ribosomal protein S4

+
Macromolecule #2: initiator tRNA-Met

MacromoleculeName: initiator tRNA-Met / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 24.255533 KDa
SequenceString:
AGCACCGUGG CGCAGUGGAA GCGCGAUGGG CUCAUAACCC AUAGGACGUU GGAUCGAAAC CAACCGGUGC UACCA

+
Macromolecule #3: 18S

MacromoleculeName: 18S / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 746.696125 KDa
SequenceString: UGAUCUGGUU GAUUCUGCCA GUAGUCAUAU GCUUGUUUCA AGGACUUAGC CAUGCAUGCC UCAGAAUCAC UGCAUUGCAG GAAUCUGCG CAUGGCUCAU UACAUCAGAC GUAAUCUGCC GCAAAAAUCU UGCGGUCUCC GCAACAUUGG AUAACUUGGC G AAACGCCA ...String:
UGAUCUGGUU GAUUCUGCCA GUAGUCAUAU GCUUGUUUCA AGGACUUAGC CAUGCAUGCC UCAGAAUCAC UGCAUUGCAG GAAUCUGCG CAUGGCUCAU UACAUCAGAC GUAAUCUGCC GCAAAAAUCU UGCGGUCUCC GCAACAUUGG AUAACUUGGC G AAACGCCA AGCUAAUACA UGAACCAACC GGAUGUUCUC UGUUCCGGCG GCAGGGCAAC CUGCUGCCAU GGGACGUCCA GC GAAUGAA UGAAAGUAAA ACCAAUGCCU UCACCGGCAG UAACACUCAG AAGUGUUGAU UCAAUUCAUU CCGUGCGAAA GCC GGGUUU UUUUAUCCGG CGUCUUUUGA CGAACAACUG CCCUAUCAGC CAGCGAUGGC CGUGUAGUGG ACUGCCAUGG CGUU GACGG GAGCGGGGGA UUAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAAUAGCUA CCACUUCUAC GGAGGGCAGC AGGCG CGCA AAUUGCCCAA UGUCAAAAAA AAAAGAUGAG GCAGCGAAAA GAAAUAGAGC CGACAGUGCU UUUGCAUUGU CGUUUU CAA UGGGGGAUAU UUAAACCCAU CCAAAAUCGA GUAACAAUUG GAGGACAAGU CUGGUGCCAG CACCCGCGGU AAUUCCA GC UCCAAAAGCG UAUAUUAAUG CUGUUGCUGU UAAAGGGUUC GUAGUUGAAU UGAGGGCCUC UAAGGCGCAA UGGUUUAG U CCCAUCCACU UCGGAUUGGU GACCCAUGCC CUUGUGGUCC GUGAACAGAC AUUCAGAAAC AAAAAACACG GGAGUGGUA CCUUUCCUGA UUAUCGCAUG UCAUGCAUGC CAGAGGGCGC CCGUGAUUUU UUACUGUGAC UAAAAAAGUG UGACCAAAGC AGUCAUUCG ACUUGAAUUA GAAAGCAUGG GAUAACAAAG GAGCAGCCUC UGGGCCACCG UUUCGGCUUU UGUUGGUUUU A AAAGUCCA UUGGAGAUUA UGGGGCAGUG UGACAAGCGG CUGGGUGGUU AUUCCACACA CACACACACA CGCUCCUUUU UU UUGGACG UGUUUUGUGU GUGUAUGUGG CACUCGUCGC CUUUGUGGGA AAUCCGUGUG GCACUGUGUU UGAUGUUGUU GGC AGAGAC UUCGGUCUUU UGCCUUCGCA UAUUUCACAC AUGUGUCAUG CCUUCCCUCA ACUCACGGCA UCCAGGAAUG AAGG AGGGU AGUUCGGGGG AGAACGUACU GGUGCGUCAG AGGUGAAAUU CUUAGACCGC ACCAAGACGA ACUACAGCGA AGGCA UUCU UCAAGGAUAC CUUCCUCAAU CAAGAACCAA AGUGUGGGGA UCGAAGAUGA UUAGAGACCA UUGUAGUCCA CACUGC AAA CGAUGACACC CAUGAAUUGG GGAGUUUUUG GUCGUAGGCG UGGUCGGGCU UGAUUAUUAU UUUUCAUCCC GUUCCUC GU CUCGCCAAUG AAUAUUAAAU UUACGUGCAU AUUCUUUUUG GUCUUCGUUU UUUUACGGCG AGGGCCUUUA ACGGGAAU A UCCUCAGCAC GUUAUCUGAC UUCUUCACGC GAAAGCUUUG AGGUUACAGU CUCAGGGGGG AGUACGUUCG CAAGAGUGA AACUUAAAGA AAUUGACGGA AUGGCACCAC AAGACGUGGA GCGUGCGGUU UAAUUUGACU CAACACGGGG AACUUUACCA GAUCCGGAC AGGGUGAGGA UUGACAGAUU GAGUGUUCUU UCUCGAUCCC CUGAAUGGUG GUGCAUGGCC GCUUUUGGUC G GUGGAGUG AUUUGUUUGG UUGAUUCCGU CAACGGACGA GAUCCAAGCU GCCCAGUAGG AUUCAGAAUU GCCCAUAGGA UA GCAAUCC CUUCCGCGGG UUUUACCCAA GGGGGGGCGG UAUUCGCUUG UAUCCUUCUC UGCGGGAUUC CUUGUUUUGC GCA AGGUGA GAUUUUGGGC AACAGCAGGU CUGUGAUGCU CCUCAAUGUU CUGGGCGACA CGCGCACUAC AAUGUCAGUG AGAA CAAGA AAAACGACUC UUGUCGGACC UACUUGAUCA AAAGAGUGGG AAAACCCCGG AAUCACGUAG ACCCACUUGG GACCG AGUA UUGCAAUUAU UGGUCGCGCA ACGAGGAAUG UCUCGUAGGC GCAGCUCAUC AAACUGUGCC GAUUACGUCC CUGCCA UUU GUACACACCG CCCGUCGUUG UUUCCGAUGA UGGUGCAAUA CAGGUGAUCG GACAGUCGAG UGCUUCACUU GACCGAA AG UUCACCGAUA UUUCUUCAAU AGAGGAAGCA AAAGUCGUAA CAAGGUAGCU GUAGGUGAAC CUGCAGCUGG AUCAUUU

GENBANK: GENBANK: FJ900241.2

+
Macromolecule #53: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 53 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #54: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #55: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 55 / Number of copies: 1 / Formula: GNP
Molecular weightTheoretical: 522.196 Da
Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
GridModel: Quantifoil R2/2 / Mesh: 300
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 30.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: OTHER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5opt

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.33 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 33775
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING

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