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- PDB-5opt: Structure of KSRP in context of Trypanosoma cruzi 40S -

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Basic information

Entry
Database: PDB / ID: 5opt
TitleStructure of KSRP in context of Trypanosoma cruzi 40S
Components
  • (40S ribosomal protein ...) x 26
  • (Ribosomal protein ...) x 5
  • 18S rRNA
  • Activated protein kinase C receptor, putative
  • RNA-binding protein, putative
  • Ubiquitin/ribosomal protein S27a, putative
KeywordsRIBOSOME / Kinetoplastids / cryo-EM / KSRP
Function / homology
Function and homology information


90S preribosome / translation regulator activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / rRNA processing / kinase activity / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly ...90S preribosome / translation regulator activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / rRNA processing / kinase activity / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytoplasmic translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / nucleolus / RNA binding / zinc ion binding / nucleus / cytoplasm / cytosol
Similarity search - Function
Ribosomal protein S26 / Ribosomal protein S17 / Ribosomal protein S4, central domain / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #1000 / Ribosomal protein S21 / Hypothetical Cytosolic Protein; Chain: A; / Ribosomal protein S27 / first zn-finger domain of poly(adp-ribose) polymerase-1 / Alpha-Beta Plaits - #3370 / Diphtheria Toxin Repressor; domain 2 ...Ribosomal protein S26 / Ribosomal protein S17 / Ribosomal protein S4, central domain / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #1000 / Ribosomal protein S21 / Hypothetical Cytosolic Protein; Chain: A; / Ribosomal protein S27 / first zn-finger domain of poly(adp-ribose) polymerase-1 / Alpha-Beta Plaits - #3370 / Diphtheria Toxin Repressor; domain 2 / Ribosomal protein S3, C-terminal domain / : / Ribosomal Protein S8; Chain: A, domain 1 - #30 / Ribosomal protein S3 C-terminal domain / Dna Ligase; domain 1 - #10 / Ribosomal protein S11/S14 / Ribosomal protein S10 / S15/NS1, RNA-binding / Ribosomal Protein S7 / Ribosomal protein S7/S5 / RNA-binding S4 domain / SH3 type barrels. - #30 / Ribosomal protein L30/S12 / Structural Genomics Hypothetical 15.5 Kd Protein In mrcA-pckA Intergenic Region; Chain A / K homology (KH) domain / Double Stranded RNA Binding Domain - #20 / Glucose-6-phosphate isomerase like protein; domain 1 / Ribosomal Protein S8; Chain: A, domain 1 / N-terminal domain of TfIIb / Ribosomal Protein S5; domain 2 - #10 / GMP Synthetase; Chain A, domain 3 / Ribosomal Protein S5; domain 2 / Double Stranded RNA Binding Domain / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / : / Ribosomal protein S12e signature. / 60s Ribosomal Protein L30; Chain: A; / Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / S27a-like superfamily / 40S Ribosomal protein S10 / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S3, eukaryotic/archaeal / Single Sheet / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S19A/S15e / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / Ribosomal protein S7e / 40S ribosomal protein S1/3, eukaryotes / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / : / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S28e conserved site / Ribosomal protein S28e signature. / Ribosomal protein S27 / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S27 / Ribosomal protein S8e / Ribosomal protein S3Ae / Ribosomal S3Ae family
Similarity search - Domain/homology
: / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS2 / 40S ribosomal protein S12 / 40S ribosomal protein S7 / Ribosomal protein S29, putative / 40S ribosomal protein S26 ...: / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS2 / 40S ribosomal protein S12 / 40S ribosomal protein S7 / Ribosomal protein S29, putative / 40S ribosomal protein S26 / 40S ribosomal protein S11, putative / Ubiquitin-ribosomal protein eL40 fusion protein / Small ribosomal subunit protein uS4 / 40S ribosomal protein S4 / Small ribosomal subunit protein uS10 / 40S ribosomal protein S14, putative / 40S ribosomal protein S33, putative / 40S ribosomal protein S30 / 40S ribosomal protein S13, putative / 40S ribosomal protein S16, putative / 40S ribosomal protein S15, putative / Small ribosomal subunit protein uS5 / Ribosomal protein S19, putative / 40S ribosomal protein S17, putative / 40S ribosomal protein S3 / Ribosomal protein S27, putative / 40S ribosomal protein S6 / Activated protein kinase C receptor homolog / 40S ribosomal protein S23, putative / 40S ribosomal protein S25 / 40S ribosomal protein S8 / 40S ribosomal protein S24 / RNA-binding protein, putative / Small ribosomal subunit protein eS1B / 40S ribosomal protein S10, putative / 40S ribosomal protein S18, putative / 40S ribosomal protein S15a, putative / 40S ribosomal protein S5, putative / 40S ribosomal protein S21, putative
Similarity search - Component
Biological speciesTrypanosoma cruzi (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4 Å
AuthorsBrito Querido, J. / Mancera-Martinez, E. / Vicens, Q. / Bochler, A. / Chicher, J. / Simonetti, A. / Hashem, Y.
Funding support France, 2items
OrganizationGrant numberCountry
French National Research AgencyANR-14-ACHN-0024 France
LABEXANR-10-LABX-0036_NETRNA France
CitationJournal: Structure / Year: 2017
Title: The cryo-EM Structure of a Novel 40S Kinetoplastid-Specific Ribosomal Protein.
Authors: Jailson Brito Querido / Eder Mancera-Martínez / Quentin Vicens / Anthony Bochler / Johana Chicher / Angelita Simonetti / Yaser Hashem /
Abstract: Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti- ...Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti-kinetoplastid therapeutic molecules that can replace the scarce and highly cytotoxic current drugs. The kinetoplastid ribosome represents a potential therapeutic target due to its relative structural divergence when compared with its human counterpart. However, several kinetoplastid-specific ribosomal features remain uncharacterized. Here, we present the near-atomic cryoelectron microscopy structure of a novel bona fide kinetoplastid-specific ribosomal (r-) protein (KSRP) bound to the ribosome. KSRP is an essential protein located at the solvent face of the 40S subunit, where it binds and stabilizes kinetoplastid-specific domains of rRNA, suggesting its role in ribosome integrity. KSRP also interacts with the r-protein eS6 at a region that is only conserved in kinetoplastids. The kinetoplastid-specific ribosomal environment of KSRP provides a promising target for the design of safer anti-kinetoplastidian drugs.
History
DepositionAug 10, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 15, 2017Provider: repository / Type: Initial release
Revision 1.1Dec 13, 2017Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2Nov 20, 2024Group: Author supporting evidence / Data collection ...Author supporting evidence / Data collection / Database references / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / em_admin / pdbx_audit_support / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _em_admin.last_update / _pdbx_audit_support.funding_organization

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Structure visualization

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Assembly

Deposited unit
p: Activated protein kinase C receptor, putative
q: Ribosomal protein S29, putative
r: 40S ribosomal protein S16, putative
t: 40S ribosomal protein S15, putative
u: 40S ribosomal protein S18, putative
L: 40S ribosomal protein S4
M: 40S ribosomal protein S23, putative
O: 40S ribosomal protein S5, putative
Q: Ribosomal protein S7, putative
R: 40S ribosomal protein S13, putative
S: 40S ribosomal protein S27, putative
T: 40S ribosomal protein S26
U: 40S ribosomal protein S33, putative
V: 40S ribosomal protein S14, putative
W: 40S ribosomal protein S3a-2
X: 40S ribosomal protein S11, putative
Y: 40S ribosomal protein S24
Z: 40S ribosomal protein S8
b: 40S ribosomal protein S9, putative
f: 40S ribosomal protein SA
d: 40S ribosomal protein S2, putative
e: 40S ribosomal protein S15a, putative
g: 40S ribosomal protein S21, putative
a: Ribosomal protein S25, putative
i: 40S ribosomal protein S12
j: Ubiquitin/ribosomal protein S27a, putative
P: 40S ribosomal protein S6
k: 40S ribosomal protein S17, putative
l: Ribosomal protein S20, putative
m: 40S ribosomal protein S3, putative
n: 40S ribosomal protein S10, putative
o: Ribosomal protein S19, putative
c: 40S ribosomal protein S30
h: RNA-binding protein, putative
E: 18S rRNA


Theoretical massNumber of molelcules
Total (without water)1,416,99235
Polymers1,416,99235
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: mass spectrometry
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area204110 Å2
ΔGint-1489 kcal/mol
Surface area433550 Å2
MethodPISA

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Components

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Protein , 3 types, 3 molecules pjh

#1: Protein Activated protein kinase C receptor, putative


Mass: 35070.312 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053511211.120, Tc00.1047053511211.130 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DTN2
#26: Protein Ubiquitin/ribosomal protein S27a, putative


Mass: 16608.434 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053510409.39 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4D1T6
#34: Protein RNA-binding protein, putative


Mass: 27676.303 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053511727.270 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DY32

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Ribosomal protein ... , 5 types, 5 molecules qQalo

#2: Protein Ribosomal protein S29, putative


Mass: 6651.826 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener
Gene: Tc00.1047053506025.14, Tc00.1047053511209.54, Tc00.1047053511805.15
Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4CXR4
#9: Protein Ribosomal protein S7, putative


Mass: 24896.504 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053506593.30 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4CUC7
#24: Protein Ribosomal protein S25, putative


Mass: 12379.955 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053504105.94, Tc00.1047053509233.190 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DTX6
#29: Protein Ribosomal protein S20, putative


Mass: 13248.478 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener
Gene: Tc00.1047053508475.10, Tc00.1047053508823.120, Tc00.1047053508823.140
Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4D6H7
#32: Protein Ribosomal protein S19, putative


Mass: 18861.287 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053510879.20 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DJY1

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40S ribosomal protein ... , 26 types, 26 molecules rtuLMORSTUVWXYZbfdegiPkmnc

#3: Protein 40S ribosomal protein S16, putative


Mass: 16841.643 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053503899.20, Tc00.1047053503899.30 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DEK4
#4: Protein 40S ribosomal protein S15, putative


Mass: 17554.453 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener
Gene: Tc00.1047053511809.130, Tc00.1047053511809.99, Tc00.1047053511811.10
Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DGZ5
#5: Protein 40S ribosomal protein S18, putative


Mass: 17585.469 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053506679.100, Tc00.1047053506679.94 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4E093
#6: Protein 40S ribosomal protein S4


Mass: 30936.521 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener
Gene: Tc00.1047053509683.117, Tc00.1047053511051.39, Tc00.1047053511051.50
Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4D5P4
#7: Protein 40S ribosomal protein S23, putative


Mass: 15960.672 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener
Gene: Tc00.1047053504181.20, Tc00.1047053504181.30, Tc00.1047053509695.180, Tc00.1047053509695.184
Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DTQ1
#8: Protein 40S ribosomal protein S5, putative


Mass: 21292.715 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053506297.150, Tc00.1047053506297.160 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4E0Q3
#10: Protein 40S ribosomal protein S13, putative


Mass: 17295.621 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053511291.90 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DC38
#11: Protein 40S ribosomal protein S27, putative / Ribosomal protein S27 / putative


Mass: 9581.131 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener
Gene: Tc00.1047053506963.14, Tc00.1047053511233.14, Tc00.1047053511277.175
Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DN73
#12: Protein 40S ribosomal protein S26


Mass: 12793.177 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053503801.20, Tc00.1047053508817.60 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4CYE4
#13: Protein 40S ribosomal protein S33, putative


Mass: 12427.547 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053511237.60 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4D7C8
#14: Protein 40S ribosomal protein S14, putative


Mass: 15596.004 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener
Gene: Tc00.1047053409117.20, Tc00.1047053506945.230, Tc00.1047053508823.50
Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4D6I5
#15: Protein 40S ribosomal protein S3a-2


Mass: 29909.693 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053511001.9, Tc00.1047053511001.18 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DZ41
#16: Protein 40S ribosomal protein S11, putative


Mass: 20018.307 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053508319.70, Tc00.1047053508405.40 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4D0U7
#17: Protein 40S ribosomal protein S24


Mass: 15706.745 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053507681.150, Tc00.1047053507681.160 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DW38
#18: Protein 40S ribosomal protein S8


Mass: 25246.783 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053511903.110 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DU83
#19: Protein 40S ribosomal protein S9, putative


Mass: 22181.932 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053504163.30, Tc00.1047053506401.120 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4D4S1
#20: Protein 40S ribosomal protein SA


Mass: 27885.129 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053510425.19 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4CQ63
#21: Protein 40S ribosomal protein S2, putative


Mass: 28574.951 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener
Gene: Tc00.1047053503833.40, Tc00.1047053506213.60, Tc00.1047053511287.120, Tc00.1047053511287.40
Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DIZ9
#22: Protein 40S ribosomal protein S15a, putative


Mass: 14683.270 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053506297.330 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4E0N6
#23: Protein 40S ribosomal protein S21, putative


Mass: 26094.570 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053510101.420 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4E3M0
#25: Protein 40S ribosomal protein S12


Mass: 15792.110 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053503395.50, Tc00.1047053506181.59 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4CSL1
#27: Protein 40S ribosomal protein S6


Mass: 28344.391 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053510769.49 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DSU0
#28: Protein 40S ribosomal protein S17, putative


Mass: 22680.713 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053508827.70 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DK40
#30: Protein 40S ribosomal protein S3, putative


Mass: 24188.436 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener
Gene: Tc00.1047053504021.109, Tc00.1047053507677.39, Tc00.1047053509353.30
Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DMK9
#31: Protein 40S ribosomal protein S10, putative


Mass: 18284.725 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053506679.140, Tc00.1047053506679.150 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4E088
#33: Protein 40S ribosomal protein S30


Mass: 7445.938 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (strain CL Brener) (eukaryote)
Strain: CL Brener / Gene: Tc00.1047053507019.83, Tc00.1047053507019.86 / Production host: Trypanosoma cruzi (eukaryote) / References: UniProt: Q4DA48

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RNA chain , 1 types, 1 molecules E

#35: RNA chain 18S rRNA


Mass: 746696.125 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma cruzi (eukaryote) / Production host: Trypanosoma cruzi (eukaryote) / References: GenBank: 320364483

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 80S ribosome from Trypanosoma cruzi / Type: RIBOSOME / Entity ID: all / Source: NATURAL
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Buffer solutionpH: 7.2
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 2.2 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 86000 / Symmetry type: POINT

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