negative regulation of protein deubiquitination / regulation of viral entry into host cell / protein K27-linked ubiquitination / suppression of viral release by host / regulation of type I interferon production / cellular response to chemical stress / negative regulation of viral transcription / protein K6-linked ubiquitination / STING mediated induction of host immune responses / stress granule disassembly ...negative regulation of protein deubiquitination / regulation of viral entry into host cell / protein K27-linked ubiquitination / suppression of viral release by host / regulation of type I interferon production / cellular response to chemical stress / negative regulation of viral transcription / protein K6-linked ubiquitination / STING mediated induction of host immune responses / stress granule disassembly / negative regulation of NF-kappaB transcription factor activity / pyroptotic inflammatory response / response to type II interferon / protein monoubiquitination / protein K63-linked ubiquitination / autophagosome / proteasomal protein catabolic process / protein autoubiquitination / protein K48-linked ubiquitination / positive regulation of autophagy / positive regulation of cell cycle / Regulation of innate immune responses to cytosolic DNA / negative regulation of innate immune response / P-body / protein destabilization / RING-type E3 ubiquitin transferase / positive regulation of DNA-binding transcription factor activity / cytoplasmic stress granule / protein polyubiquitination / ubiquitin-protein transferase activity / regulation of protein localization / Interferon gamma signaling / KEAP1-NFE2L2 pathway / ubiquitin protein ligase activity / Antigen processing: Ubiquitination & Proteasome degradation / positive regulation of protein binding / cytoplasmic vesicle / positive regulation of canonical NF-kappaB signal transduction / transcription coactivator activity / positive regulation of viral entry into host cell / protein ubiquitination / ribonucleoprotein complex / cell cycle / innate immune response / protein kinase binding / protein homodimerization activity / DNA binding / RNA binding / zinc ion binding / nucleoplasm / identical protein binding / nucleus / cytoplasm / cytosol Similarity search - Function
Resolution: 1.95→2 Å / Redundancy: 7.5 % / Rmerge(I) obs: 0.9 / Mean I/σ(I) obs: 2.2 / % possible all: 99.4
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0158
refinement
iMOSFLM
datareduction
Aimless
datascaling
PHENIX
phasing
Refinement
Method to determine structure: SAD / Resolution: 1.95→67.95 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.928 / SU B: 5.324 / SU ML: 0.138 / Cross valid method: THROUGHOUT / ESU R: 0.172 / ESU R Free: 0.166 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.26162
1025
4.9 %
RANDOM
Rwork
0.20833
-
-
-
obs
0.21087
19875
98.59 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK