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Open data
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Basic information
Entry | Database: PDB / ID: 1jcf | ||||||
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Title | MREB FROM THERMOTOGA MARITIMA, TRIGONAL | ||||||
![]() | ROD SHAPE-DETERMINING PROTEIN MREB | ||||||
![]() | STRUCTURAL PROTEIN / MreB / rod-shape determining / Mbl / actin / hsp-70 / FtsZ | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | van den Ent, F. / Amos, L.A. / Lowe, J. | ||||||
![]() | ![]() Title: Prokaryotic origin of the actin cytoskeleton. Authors: van den Ent, F. / Amos, L.A. / Lowe, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82 KB | Display | ![]() |
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PDB format | ![]() | 60.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1jceSC ![]() 1jcgC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | Crystal packing generates one-dimensional filaments alon a and b, similar to one strand in F-actin (protofilament) |
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Components
#1: Protein | Mass: 36799.582 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 58.98 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 19 ℃ / pH: 10.5 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: May 8, 2001 |
Radiation | Monochromator: double / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9393 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→50 Å / Num. all: 27249 / Num. obs: 26139 / % possible obs: 95.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Biso Wilson estimate: 25 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 2.09→2.2 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.115 / % possible all: 92.4 |
Reflection | *PLUS Rmerge(I) obs: 0.06 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1JCE Resolution: 2.1→50 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: CNS 1.0, protein_rep.param
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Displacement parameters | Biso mean: 30.31 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→50 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 50 Å / σ(F): 0 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |