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- PDB-1ijj: THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETA... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ijj | ||||||
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Title | THE X-RAY CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RABBIT SKELETAL MUSCLE ACTIN AND LATRUNCULIN A AT 2.85 A RESOLUTION | ||||||
![]() | ACTIN, ALPHA SKELETAL MUSCLE | ||||||
![]() | CONTRACTILE PROTEIN / actin / latrunculin / cytoskeleton | ||||||
Function / homology | ![]() cytoskeletal motor activator activity / tropomyosin binding / mesenchyme migration / troponin I binding / myosin heavy chain binding / filamentous actin / actin filament bundle / skeletal muscle thin filament assembly / striated muscle thin filament / actin filament bundle assembly ...cytoskeletal motor activator activity / tropomyosin binding / mesenchyme migration / troponin I binding / myosin heavy chain binding / filamentous actin / actin filament bundle / skeletal muscle thin filament assembly / striated muscle thin filament / actin filament bundle assembly / skeletal muscle myofibril / actin monomer binding / skeletal muscle fiber development / stress fiber / titin binding / actin filament polymerization / filopodium / actin filament / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / calcium-dependent protein binding / lamellipodium / cell body / hydrolase activity / protein domain specific binding / calcium ion binding / positive regulation of gene expression / magnesium ion binding / ATP binding / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Vorobiev, S.M. / Bubb, M.R. / Almo, S.C. | ||||||
![]() | ![]() Title: Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution Authors: Bubb, M.R. / Govindasamy, L. / Yarmola, E.G. / Vorobiev, S.M. / Almo, S.C. / Somasundaram, T. / Chapman, M.S. / Agbandje-McKenna, M. / McKenna, R. #1: ![]() Title: Actin-latrunculin A structure and function. Differential modulation of actin-binding protein function by latrunculin A Authors: Yarmola, E.G. / Somasundaram, T. / Boring, T.A. / Spector, I. / Bubb, M.R. #2: ![]() Title: Latrunculin alters the actin-monomer subunit interface to prevent polymerization Authors: Morton, W.M. / Ayscough, K.R. / McLaughlin, P.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 158.8 KB | Display | ![]() |
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PDB format | ![]() | 121 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 663.7 KB | Display | ![]() |
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Full document | ![]() | 732.4 KB | Display | |
Data in XML | ![]() | 26.2 KB | Display | |
Data in CIF | ![]() | 37.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1lcuC ![]() 1yagS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 42096.953 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.78 Å3/Da / Density % sol: 67.46 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.8 Details: ammonium sulfate, magnesium chloride, pH 6.8, VAPOR DIFFUSION, HANGING DROP at 298 K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.984 Å / Relative weight: 1 |
Reflection | Resolution: 2.85→30 Å / Num. obs: 29997 / % possible obs: 98.7 % / Redundancy: 4 % / Rmerge(I) obs: 0.049 |
Reflection shell | Resolution: 2.85→2.95 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.527 / Num. unique all: 2969 / % possible all: 99.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1YAG Resolution: 2.85→15 Å / Isotropic thermal model: RESTRAINED / σ(F): 2
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Displacement parameters |
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Refine analyze | Luzzati coordinate error obs: 0.48 Å | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.85→15 Å
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Refine LS restraints |
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