+Open data
-Basic information
Entry | Database: PDB / ID: 4cze | |||||||||
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Title | C. crescentus MreB, double filament, empty | |||||||||
Components | ROD SHAPE-DETERMINING PROTEIN MREB | |||||||||
Keywords | STRUCTURAL PROTEIN / BACTERIAL ACTIN / BACTERIAL CYTOSKELETON | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | CAULOBACTER VIBRIOIDES (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | |||||||||
Authors | Lowe, J. / van den Ent, F. | |||||||||
Citation | Journal: Elife / Year: 2014 Title: Bacterial Actin Mreb Forms Antiparallel Double Filaments. Authors: Van Den Ent, F. / Izore, T. / Bharat, T.A. / Johnson, C.M. / Lowe, J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4cze.cif.gz | 81.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4cze.ent.gz | 58.9 KB | Display | PDB format |
PDBx/mmJSON format | 4cze.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cz/4cze ftp://data.pdbj.org/pub/pdb/validation_reports/cz/4cze | HTTPS FTP |
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-Related structure data
Related structure data | 4czfC 4czgC 4czhC 4cziC 4czjC 4czkC 4czlC 4czmC 1jceS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 36967.672 Da / Num. of mol.: 1 / Fragment: RESIDUES 9-347 Source method: isolated from a genetically manipulated source Details: M-I9-CCMREB-A347-GSHHHHHH / Source: (gene. exp.) CAULOBACTER VIBRIOIDES (bacteria) / Plasmid: PHIS17 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): C41 / References: UniProt: B8H609, UniProt: A0A0H3C7V4*PLUS |
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#2: Chemical | ChemComp-PO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 60.34 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.9173 |
Detector | Type: DECTRIS PILATUS / Detector: PIXEL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9173 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. obs: 29232 / % possible obs: 96.1 % / Observed criterion σ(I): 0 / Redundancy: 3.2 % / Biso Wilson estimate: 23 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 7 |
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.38 / Mean I/σ(I) obs: 3.3 / % possible all: 98.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1JCE Resolution: 2→34.241 Å / SU ML: 0.43 / σ(F): 0.86 / Phase error: 21.3 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 49.225 Å2 / ksol: 0.396 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2→34.241 Å
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Refine LS restraints |
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LS refinement shell |
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