- PDB-5noc: Solution NMR Structure of the C-terminal domain of ParB (Spo0J) -
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Basic information
Entry
Database: PDB / ID: 5noc
Title
Solution NMR Structure of the C-terminal domain of ParB (Spo0J)
Components
Stage 0 sporulation protein J
Keywords
DNA BINDING PROTEIN / homodimer / bacterial / chromosome / segregation / centromere
Function / homology
Function and homology information
positive regulation of sporulation resulting in formation of a cellular spore / bacterial nucleoid / sporulation resulting in formation of a cellular spore / chromosome segregation / chromosome / DNA binding Similarity search - Function
ParB/Spo0J, HTH domain / HTH domain found in ParB protein / ParB/RepB/Spo0J partition protein / ParB/Sulfiredoxin domain / ParB/Sulfiredoxin / ParB-like nuclease domain / ParB/Sulfiredoxin superfamily Similarity search - Domain/homology
3 mM [U-13C; U-15N] ParB (Spo0J), 137 mM sodium chloride, 2.7 mM potassium chloride, 10 mM Na2HPO4, 1.8 mM KH2PO4, 95% H2O/5% D2O
15N13C
95% H2O/5% D2O
solution
3
1.5 mM ParB (Spo0J), 1.5 mM [U-13C; U-15N] ParB (Spo0J), 137 mM sodium chloride, 2.7 mM potassium chloride, 10 mM Na2HPO4, 1.8 mM KH2PO4, 95% H2O/5% D2O
mixed_labelled
95% H2O/5% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
3mM
ParB (Spo0J)
naturalabundance
1
137mM
sodiumchloride
naturalabundance
1
2.7mM
potassiumchloride
naturalabundance
1
10mM
Na2HPO4
naturalabundance
1
1.8mM
KH2PO4
naturalabundance
1
3mM
ParB (Spo0J)
[U-13C; U-15N]
2
137mM
sodiumchloride
naturalabundance
2
2.7mM
potassiumchloride
naturalabundance
2
10mM
Na2HPO4
naturalabundance
2
1.8mM
KH2PO4
naturalabundance
2
1.5mM
ParB (Spo0J)
naturalabundance
3
1.5mM
ParB (Spo0J)-label
[U-13C; U-15N]
3
137mM
sodiumchloride
naturalabundance
3
2.7mM
potassiumchloride
naturalabundance
3
10mM
Na2HPO4
naturalabundance
3
1.8mM
KH2PO4
naturalabundance
3
Sample conditions
Ionic strength: 150 mM / Label: standard / pH: 6.1 / Pressure: 1 atm / Temperature: 208 K
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