|Entry||Database: PDB / ID: 5nd1|
|Title||Viral evolution results in multiple, surface-allocated enzymatic activities in a fungal double-stranded RNA virus|
|Keywords||VIRUS / RnQV1 / dsRNA virus / fungal virus / virus|
|Specimen source||Rosellinia necatrix quadrivirus 1 / virus / RnQV1|
|Method||Electron microscopy (3.7 Å resolution / Particle / Single particle)|
|Authors||Mata, C.P. / Luque, D. / Gomez Blanco, J. / Rodriguez, J.M. / Suzuki, N. / Ghabrial, S.A. / Carrascosa, J.L. / Trus, B.L. / Caston, J.R.|
|Citation||To Be Published|
To Be Published Search PubMed
SummaryFull reportAbout validation report
|Date||Deposition: Mar 7, 2017 / Release: Nov 29, 2017|
Downloads & links
A: Capsid protein
B: Capsid protein
Mass: 147782.375 Da / Num. of mol.: 1 / Source: (natural) Rosellinia necatrix quadrivirus 1 / virus / References: UniProt: M1VMJ0
|Experiment||Method: ELECTRON MICROSCOPY|
|EM experiment||Aggregation state: PARTICLE / Reconstruction method: SINGLE PARTICLE|
|Component||Name: virus / Type: VIRUS / Entity ID: 1, 2|
|Molecular weight||Value: 15.9 deg. / Units: MEGADALTONS / Experimental value: YES|
|Details of virus||Empty: YES / Enveloped: NO / Virus isolate: STRAIN / Virus type: VIRION|
|Natural host||Organism: Rosellinia necatrix|
|Virus shell||Name: P2 and P4 capsid proteins / Diameter: 470 Å / Triangulation number (T number): 1|
|Buffer solution||Details: 50 mM Tris-HCl pH 7.8, 150 mM NaCl, 5 mM EDTA / pH: 7.8|
|Specimen||Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES|
|Specimen support||Grid material: COPPER/RHODIUM / Grid mesh size: 300 / Grid type: Quantifoil R2/2|
|Vitrification||Instrument: LEICA EM CPC / Cryogen name: ETHANE|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Microscopy||Microscope model: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM|
|Electron lens||Mode: BRIGHT FIELD / Calibrated defocus min: 700 nm / Calibrated defocus max: 3500 nm / Cs: 2.7 mm|
|Specimen holder||Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER|
|Image recording||Average exposure time: 2 sec. / Electron dose: 1.7 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)|
|Image scans||Movie frames/image: 24 / Used frames/image: 4-21|
|CTF correction||Type: PHASE FLIPPING ONLY|
|Particle selection||Number of particles selected: 53683|
|3D reconstruction||Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 37531 / Symmetry type: POINT|
|Atomic model building||Ref protocol: BACKBONE TRACE|
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Three pioneers of this field were awarded Nobel Prize in Chemistry 2017
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External links: The 2017 Nobel Prize in Chemistry - Press Release
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