+Open data
-Basic information
Entry | Database: PDB / ID: 4m8n | ||||||
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Title | Crystal Structure of PlexinC1/Rap1B Complex | ||||||
Components |
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Keywords | SIGNALING PROTEIN / GTPase / GTPase activating protein / Rap / GTP binding / Magnesium Binding / Membrane | ||||||
Function / homology | Function and homology information Rap protein signal transduction / modification of postsynaptic structure / regulation of cell junction assembly / negative regulation of calcium ion-dependent exocytosis / positive regulation of integrin activation / negative regulation of synaptic vesicle exocytosis / calcium-ion regulated exocytosis / semaphorin receptor activity / Rap1 signalling / establishment of endothelial barrier ...Rap protein signal transduction / modification of postsynaptic structure / regulation of cell junction assembly / negative regulation of calcium ion-dependent exocytosis / positive regulation of integrin activation / negative regulation of synaptic vesicle exocytosis / calcium-ion regulated exocytosis / semaphorin receptor activity / Rap1 signalling / establishment of endothelial barrier / regulation of establishment of cell polarity / MET activates RAP1 and RAC1 / azurophil granule membrane / p130Cas linkage to MAPK signaling for integrins / GRB2:SOS provides linkage to MAPK signaling for Integrins / cellular response to cAMP / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / lipid droplet / Integrin signaling / small monomeric GTPase / G protein activity / axon guidance / establishment of localization in cell / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / GDP binding / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / cell-cell junction / Signaling by BRAF and RAF1 fusions / cell population proliferation / positive regulation of ERK1 and ERK2 cascade / GTPase activity / glutamatergic synapse / Neutrophil degranulation / protein-containing complex binding / GTP binding / extracellular exosome / membrane / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Danio rerio (zebrafish) Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3.294 Å | ||||||
Authors | Pascoe, H.G. / Wang, Y. / Brautigam, C.A. / He, H. / Zhang, X. | ||||||
Citation | Journal: Elife / Year: 2013 Title: Structural basis for activation and non-canonical catalysis of the Rap GTPase activating protein domain of plexin. Authors: Wang, Y. / Pascoe, H.G. / Brautigam, C.A. / He, H. / Zhang, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4m8n.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb4m8n.ent.gz | 969.8 KB | Display | PDB format |
PDBx/mmJSON format | 4m8n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m8/4m8n ftp://data.pdbj.org/pub/pdb/validation_reports/m8/4m8n | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 2 types, 8 molecules ABCDEFGH
#1: Protein | Mass: 69216.141 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Danio rerio (zebrafish) / Gene: Plexin C1, plxnc1 / Plasmid: modified pET28 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(Arctic Express) / References: UniProt: Q5RGW1 #2: Protein | Mass: 21409.752 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: OK/SW-cl.11, Rap 1B, RAP1B / Plasmid: modified pET28 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) / References: UniProt: P61224 |
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-Non-polymers , 4 types, 22 molecules
#3: Chemical | ChemComp-MG / #4: Chemical | ChemComp-GDP / #5: Chemical | ChemComp-AF3 / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.32 % |
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Crystal grow | Temperature: 294.15 K / Method: vapor diffusion, hanging drop / pH: 7.3 Details: 0.1 M HEPES, 5% 2-propanol, 25% PEG 3350, 3.6% polypropylene glycol P400, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97926 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 13, 2013 |
Radiation | Monochromator: si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97926 Å / Relative weight: 1 |
Reflection | Resolution: 3.294→50 Å / Num. all: 51910 / Num. obs: 47245 / % possible obs: 91 % / Observed criterion σ(I): -3 / Redundancy: 1.9 % / Biso Wilson estimate: 106.4 Å2 / Rsym value: 0.052 / Net I/σ(I): 18.6 |
Reflection shell | Resolution: 3.294→3.36 Å / Redundancy: 1.9 % / Mean I/σ(I) obs: 1.6 / Rsym value: 0.487 / % possible all: 89.2 |
-Processing
Software |
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Refinement | Resolution: 3.294→41.053 Å / SU ML: 0.56 / σ(F): 1.98 / Phase error: 35.31 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.294→41.053 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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