- PDB-5n78: Crystal structure of the cytosolic domain of the CorA Mg2+ channe... -
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Basic information
Entry
Database: PDB / ID: 5n78
Title
Crystal structure of the cytosolic domain of the CorA Mg2+ channel from Escherichia coli in complex with magnesium and cobalt hexammine
Components
Magnesium transport protein CorA
Keywords
TRANSPORT PROTEIN / Homopentamer Complex Transport Membrane
Function / homology
Function and homology information
nickel cation transmembrane transport / nickel cation transmembrane transporter activity / magnesium ion transport / cobalt ion transport / cobalt ion transmembrane transporter activity / magnesium ion transmembrane transporter activity / plasma membrane Similarity search - Function
Magnesium/cobalt transport protein CorA / CorA, cytoplasmic domain / CorA, transmembrane region / Mg2+ transporter protein, CorA-like/Zinc transport protein ZntB / CorA-like Mg2+ transporter protein Similarity search - Domain/homology
A: Magnesium transport protein CorA B: Magnesium transport protein CorA C: Magnesium transport protein CorA D: Magnesium transport protein CorA E: Magnesium transport protein CorA hetero molecules
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
ID
Wavelength (Å)
Relative weight
1
0.979
1
2
1.6049
1
Reflection
Resolution: 2.85→89.45 Å / Num. obs: 35009 / % possible obs: 98.2 % / Redundancy: 5.7 % / CC1/2: 0.997 / Rmerge(I) obs: 0.121 / Net I/σ(I): 9.7
Reflection shell
Resolution: 2.85→3 Å / Rmerge(I) obs: 0.91 / CC1/2: 0.671 / % possible all: 89.4
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0073
refinement
XDS
datareduction
SCALA
datascaling
PHASER
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.85→29.44 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.942 / SU B: 34.296 / SU ML: 0.293 / Cross valid method: THROUGHOUT / ESU R Free: 0.36 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.21772
1769
5.1 %
RANDOM
Rwork
0.17761
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obs
0.17969
33216
98.14 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK