Mass: 18.015 Da / Num. of mol.: 483 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.38 Å3/Da / Density % sol: 48.3 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 12 mg/mL protein stock in mixed 1:1 with 1.2 M NaH2PO4/K2HPO4 (1:9 v/v) at 293 K.
Resolution: 1.24→28.88 Å / Cor.coef. Fo:Fc: 0.979 / Cor.coef. Fo:Fc free: 0.97 / SU B: 1.48 / SU ML: 0.028 / Cross valid method: THROUGHOUT / ESU R: 0.037 / ESU R Free: 0.038 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.16028
6276
5 %
RANDOM
Rwork
0.12933
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obs
0.13084
120177
89.59 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å