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- PDB-5knm: Human leukocyte antigen F (HLA-F) presents peptides and regulates... -

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Basic information

Entry
Database: PDB / ID: 5knm
TitleHuman leukocyte antigen F (HLA-F) presents peptides and regulates immunity through interactions with NK-cell receptors
Components
  • Beta-2-microglobulin
  • Leukocyte immunoglobulin-like receptor subfamily B member 1
  • Peptide ILE-LEU-ARG-TRP-GLU-GLN
  • cDNA FLJ39643 fis, clone SMINT2004023, highly similar to HLA class I histocompatibility antigen, alphachain F
KeywordsPROTEIN BINDING / MHC-Ib / IMMUNE SYSTEM / PEPTIDE BINDING PROTEIN
Function / homology
Function and homology information


negative regulation of natural killer cell cytokine production / negative regulation of natural killer cell degranulation / HLA-A specific inhibitory MHC class I receptor activity / positive regulation of gamma-delta T cell activation involved in immune response / negative regulation of serotonin secretion / MHC class Ib protein complex binding / positive regulation of natural killer cell degranulation / HLA-B specific inhibitory MHC class I receptor activity / immune response-inhibiting cell surface receptor signaling pathway / inhibitory MHC class I receptor activity ...negative regulation of natural killer cell cytokine production / negative regulation of natural killer cell degranulation / HLA-A specific inhibitory MHC class I receptor activity / positive regulation of gamma-delta T cell activation involved in immune response / negative regulation of serotonin secretion / MHC class Ib protein complex binding / positive regulation of natural killer cell degranulation / HLA-B specific inhibitory MHC class I receptor activity / immune response-inhibiting cell surface receptor signaling pathway / inhibitory MHC class I receptor activity / negative regulation of dendritic cell differentiation / Fc receptor mediated inhibitory signaling pathway / antigen processing and presentation of exogenous peptide antigen via MHC class Ib / MHC class Ib protein complex / MHC class Ib receptor activity / negative regulation of T cell cytokine production / positive regulation of natural killer cell cytokine production / TAP2 binding / TAP1 binding / MHC class Ib protein binding / negative regulation of T cell mediated cytotoxicity / immune response-regulating signaling pathway / negative regulation of CD8-positive, alpha-beta T cell activation / MHC class I receptor activity / negative regulation of transforming growth factor beta production / negative regulation of alpha-beta T cell activation / negative regulation of cytokine production involved in immune response / dendritic cell differentiation / interleukin-10-mediated signaling pathway / negative regulation of osteoclast development / protein phosphatase 1 binding / negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell / negative regulation of interleukin-12 production / negative regulation of dendritic cell apoptotic process / negative regulation of endocytosis / negative regulation of interferon-beta production / negative regulation of mononuclear cell proliferation / negative regulation of natural killer cell mediated cytotoxicity / T cell proliferation involved in immune response / positive regulation of macrophage cytokine production / negative regulation of interleukin-10 production / beta-2-microglobulin binding / negative regulation of calcium ion transport / negative regulation of cell cycle / MHC class I protein binding / negative regulation of type II interferon production / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / negative regulation of tumor necrosis factor production / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / negative regulation of T cell proliferation / positive regulation of defense response to virus by host / 14-3-3 protein binding / SH2 domain binding / negative regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / transferrin transport / cellular response to iron ion / Endosomal/Vacuolar pathway / lumenal side of endoplasmic reticulum membrane / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide antigen assembly with MHC class II protein complex / cellular response to iron(III) ion / MHC class II protein complex / negative regulation of forebrain neuron differentiation / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of iron ion transport / regulation of erythrocyte differentiation / HFE-transferrin receptor complex / response to molecule of bacterial origin / MHC class I peptide loading complex / T cell mediated cytotoxicity / positive regulation of T cell cytokine production / antigen processing and presentation of endogenous peptide antigen via MHC class I / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / MHC class I protein complex / positive regulation of T cell activation / peptide antigen binding / positive regulation of receptor-mediated endocytosis / negative regulation of neurogenesis / receptor internalization / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / multicellular organismal-level iron ion homeostasis / response to virus / specific granule lumen / positive regulation of type II interferon production / phagocytic vesicle membrane / recycling endosome membrane / Interferon gamma signaling / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / negative regulation of epithelial cell proliferation / MHC class II protein complex binding / Interferon alpha/beta signaling / Modulation by Mtb of host immune system / late endosome membrane
Similarity search - Function
: / Immunoglobulin domain / Immunoglobulin / Immunoglobulin domain / MHC class I-like antigen recognition-like / Murine Class I Major Histocompatibility Complex, H2-DB; Chain A, domain 1 / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type ...: / Immunoglobulin domain / Immunoglobulin / Immunoglobulin domain / MHC class I-like antigen recognition-like / Murine Class I Major Histocompatibility Complex, H2-DB; Chain A, domain 1 / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin subtype / Immunoglobulin / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold / Immunoglobulins / Immunoglobulin-like / Sandwich / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
cDNA FLJ39643 fis, clone SMINT2004023, highly similar to HLA class I histocompatibility antigen, alphachain F / HLA class I histocompatibility antigen, alpha chain F / Beta-2-microglobulin / Leukocyte immunoglobulin-like receptor subfamily B member 1
Similarity search - Component
Biological speciesHomo sapiens (human)
Trichoplusia ni (cabbage looper)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.3 Å
AuthorsDulberger, C.L. / Adams, E.J.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI073922 United States
CitationJournal: Immunity / Year: 2017
Title: Human Leukocyte Antigen F Presents Peptides and Regulates Immunity through Interactions with NK Cell Receptors.
Authors: Dulberger, C.L. / McMurtrey, C.P. / Holzemer, A. / Neu, K.E. / Liu, V. / Steinbach, A.M. / Garcia-Beltran, W.F. / Sulak, M. / Jabri, B. / Lynch, V.J. / Altfeld, M. / Hildebrand, W.H. / Adams, E.J.
History
DepositionJun 28, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 14, 2017Provider: repository / Type: Initial release
Revision 1.1Jul 5, 2017Group: Database references / Category: citation / citation_author
Item: _citation.journal_abbrev / _citation.journal_id_CSD ..._citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Sep 27, 2017Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3Dec 11, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4Jul 29, 2020Group: Data collection / Derived calculations / Structure summary
Category: chem_comp / entity ...chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / struct_site / struct_site_gen
Item: _chem_comp.name / _chem_comp.type ..._chem_comp.name / _chem_comp.type / _entity.pdbx_description / _pdbx_entity_nonpoly.name
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 1.5Sep 27, 2023Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.6Oct 16, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: cDNA FLJ39643 fis, clone SMINT2004023, highly similar to HLA class I histocompatibility antigen, alphachain F
B: Beta-2-microglobulin
D: Leukocyte immunoglobulin-like receptor subfamily B member 1
N: Peptide ILE-LEU-ARG-TRP-GLU-GLN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)83,3635
Polymers83,1414
Non-polymers2211
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)231.820, 231.820, 73.250
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number177
Space group name H-MP622

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Components

#1: Protein cDNA FLJ39643 fis, clone SMINT2004023, highly similar to HLA class I histocompatibility antigen, alphachain F


Mass: 32769.074 Da / Num. of mol.: 1 / Fragment: UNP residues 22-305
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pACgp67a / Cell (production host): High Five / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: B3KUD8, UniProt: P30511*PLUS
#2: Protein Beta-2-microglobulin


Mass: 20060.281 Da / Num. of mol.: 1 / Fragment: UNP rrsidues 21-119
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Plasmid: pACgp67a / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P61769
#3: Protein Leukocyte immunoglobulin-like receptor subfamily B member 1 / Leukocyte immunoglobulin-like receptor 1 / CD85 antigen-like family member J / Immunoglobulin-like ...Leukocyte immunoglobulin-like receptor 1 / CD85 antigen-like family member J / Immunoglobulin-like transcript 2 / ILT-2 / Monocyte/macrophage immunoglobulin-like receptor 7 / MIR-7


Mass: 29467.047 Da / Num. of mol.: 1 / Fragment: UNP residues 24-221
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: LILRB1, ILT2, LIR1, MIR7 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q8NHL6
#4: Protein/peptide Peptide ILE-LEU-ARG-TRP-GLU-GLN


Mass: 844.977 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trichoplusia ni (cabbage looper) / Production host: Trichoplusia ni (cabbage looper)
#5: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.42 Å3/Da / Density % sol: 67.59 % / Description: hexagonal plates
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: 27% PEG 550 MME .1 M Tris-HCl 300 mM NaCl

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.03318 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 20, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.03318 Å / Relative weight: 1
ReflectionResolution: 3.304→49.406 Å / Num. obs: 17859 / % possible obs: 99 % / Redundancy: 9.4 % / CC1/2: 0.997 / Rmerge(I) obs: 0.1413 / Net I/σ(I): 1.57
Reflection shellResolution: 3.304→3.422 Å / Redundancy: 9.8 % / Rmerge(I) obs: 1.136 / Mean I/σ(I) obs: 1.57 / CC1/2: 0.801 / % possible all: 99

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Processing

Software
NameVersionClassification
PHENIX(1.10_2155: ???)refinement
xia2data reduction
xia2data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1PSA

1psa


Resolution: 3.3→49.41 Å / SU ML: 0.56 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 39.49 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.326 915 5.12 %
Rwork0.303 --
obs0.305 17858 99.7 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.3→49.41 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4514 0 14 0 4528
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0034653
X-RAY DIFFRACTIONf_angle_d0.7746337
X-RAY DIFFRACTIONf_dihedral_angle_d8.0253753
X-RAY DIFFRACTIONf_chiral_restr0.044664
X-RAY DIFFRACTIONf_plane_restr0.006836
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.3036-3.47780.37171230.42982349X-RAY DIFFRACTION99
3.4778-3.69560.40681250.40132362X-RAY DIFFRACTION100
3.6956-3.98080.39161290.38352377X-RAY DIFFRACTION100
3.9808-4.38120.33981470.332380X-RAY DIFFRACTION100
4.3812-5.01460.32751520.28582384X-RAY DIFFRACTION100
5.0146-6.31580.33371090.30052472X-RAY DIFFRACTION100
6.3158-49.41190.28521300.25472619X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.59792.45851.95676.24112.76872.7260.6157-1.17853.0290.3759-0.7581.7697-0.727-0.76790.25111.19370.07510.07871.4993-0.62524.0996-74.1984136.9749121.3148
22.6639-0.3047-3.15924.5589-3.33736.7692-0.5007-2.63461.31731.7834-1.69330.9970.8995-1.16930.78811.2103-0.3412-0.00982.1009-1.06122.2853-64.846134.0467125.1442
35.93436.5216-3.82437.619-2.86596.5593-1.0408-2.07250.0470.35510.1807-1.395-0.39840.82181.18121.0693-0.0217-0.02281.6601-0.88043.1647-67.8627132.591120.222
42.56173.27850.40136.14232.55662.2088-1.60090.39021.6162-3.34020.72741.752-1.17250.0813-0.20682.2580.0467-0.35430.4760.81235.9471-62.6398148.1568100.8539
50.3295-0.7368-0.45114.24621.94480.97930.99451.6442.4761-1.4021-0.99762.3714-0.93850.2479-0.16231.8756-0.3602-0.36681.4841.05323.4953-71.366146.492790.9939
63.49330.3843-0.30656.1463.71036.50440.1476-0.29241.5016-2.2087-0.37481.1929-2.01360.88190.36391.9997-0.6836-1.2061.76530.56693.0136-69.3275139.767596.084
75.6079-0.18071.07919.45322.32725.5434-0.4183-1.57333.06960.95930.5451-1.48670.435-1.5257-0.15711.63790.1607-0.54812.05160.01343.4386-77.4008148.425696.4088
86.6357-5.70131.58114.7504-1.22035.6775-1.40280.13660.25720.26151.03032.0195-1.35490.5838-0.0321.9374-0.2361-0.3341.54620.4373.9422-65.0513145.7224101.8526
96.9135-6.80063.48438.9954-1.50042.7994-1.16610.32223.0736-0.7931.0855-1.0122-0.61350.00360.17882.0651-0.73560.35511.78280.45222.5657-27.3322141.681692.9197
100.07770.24930.44690.85611.61862.9163-0.61670.40830.3635-0.3390.09230.0009-1.99530.0676-0.99452.6125-1.26520.08272.24191.19954.1007-27.9779148.147282.3581
112.3414-1.8080.94293.00140.31322.67480.28942.04512.069-1.307-0.2348-1.2736-0.5335-0.3064-0.14932.6279-0.78180.13962.1330.82292.7252-37.9086137.788282.3442
128.81511.54595.62162.0342.31864.51760.16891.3526-0.4393-2.30462.64891.98280.52792.008-2.47513.7876-1.8754-0.63634.24721.23383.0666-39.8656138.203969.6337
139.03552.0399-2.57354.9354-0.54835.3550.74751.7211.3541-2.02260.1056-1.2841-1.3580.3828-0.66891.81890.11030.47921.23280.06361.8345-31.1896125.711694.2896
147.84051.381-4.14525.17211.68044.6879-0.57791.78880.9757-1.1736-0.17341.5664-0.6653-1.53770.4951.1719-0.2463-0.17421.08610.20781.5124-48.8618119.0403105.9748
153.2817-2.1511-0.5442.02170.53317.5217-0.54090.81741.4866-1.10460.66910.3767-0.2238-0.6604-0.13081.0348-0.1546-0.10250.7735-0.1822.0579-47.8564121.4954108.3889
169.3158-3.8306-3.66177.25435.40625.2106-0.6112-1.3226-1.26410.4290.13752.24491.14990.66940.13740.8727-0.06-0.01260.75250.34221.2899-41.8953112.5073109.2807
176.9263-2.23320.85965.91893.92483.5610.55261.44691.7919-2.5754-0.09710.7689-1.8085-0.567-0.51211.6365-1.15910.03261.3337-0.6484.1841-49.1473142.899296.3834
188.9559-0.3124-7.46588.4021-1.83496.86850.3448-1.71943.55690.38570.41411.47610.16272.2481-0.69571.234-0.2633-0.28391.3826-0.39351.8321-35.9771134.0754115.2108
198.2703-0.8216-0.13553.1014-2.79383.62840.285-0.15954.9498-0.65571.266-0.3678-2.2341-0.6612-1.5361.3959-0.1792-0.06911.17260.20113.3533-43.2309146.901299.9062
207.04040.2367-0.02314.8736-6.31168.3390.6838-0.74882.55281.8891-0.5084-1.3837-3.9292.3479-0.15981.7736-0.44060.00171.7721-1.03262.9992-39.2831148.3585118.5458
215.4839-2.26844.63983.82811.87429.284-1.1286-1.21092.7586-0.40840.6592-1.83280.3294-0.33440.84831.7128-0.6121-0.11051.12740.05133.3656-32.7658143.9876103.6779
220.24730.0256-0.13331.04750.19830.58270.07310.6056-0.3639-0.9357-0.14240.35830.0238-0.4586-0.25372.2593-2.0584-0.23141.10332.57032.4747-41.9187137.818691.9141
239.145-1.38752.8963.6374-0.29010.93440.2574-4.40120.48371.74820.51230.7627-1.0338-0.2270.06790.8134-1.1415-0.19121.0493-0.60762.5177-36.4851140.0991118.1041
249.6281-4.71043.18549.13-5.74593.9848-1.3392-0.30593.936-0.47951.69032.9338-1.6932-1.26250.24861.4407-0.1304-0.10671.04290.20463.9444-47.7932148.4359107.7661
251.33782.02-2.17668.1267-2.61113.6571.2538-0.17721.6710.3040.41240.8912-0.23472.4949-1.67261.2541-0.2029-0.03561.5868-0.51812.4484-45.7889136.5957116.8338
266.40262.8012.6634.92193.33162.3764-0.18860.5576-0.44181.1930.47512.32482.1991-1.89640.47853.8594-1.7155-0.19172.17491.46695.6672-28.1726142.239777.3012
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN 'D' AND (RESID 1 THROUGH 34 )
2X-RAY DIFFRACTION2CHAIN 'D' AND (RESID 35 THROUGH 57 )
3X-RAY DIFFRACTION3CHAIN 'D' AND (RESID 58 THROUGH 92 )
4X-RAY DIFFRACTION4CHAIN 'D' AND (RESID 93 THROUGH 106 )
5X-RAY DIFFRACTION5CHAIN 'D' AND (RESID 107 THROUGH 133 )
6X-RAY DIFFRACTION6CHAIN 'D' AND (RESID 134 THROUGH 155 )
7X-RAY DIFFRACTION7CHAIN 'D' AND (RESID 156 THROUGH 174 )
8X-RAY DIFFRACTION8CHAIN 'D' AND (RESID 175 THROUGH 195 )
9X-RAY DIFFRACTION9CHAIN 'A' AND (RESID 1 THROUGH 56 )
10X-RAY DIFFRACTION10CHAIN 'A' AND (RESID 57 THROUGH 85 )
11X-RAY DIFFRACTION11CHAIN 'A' AND (RESID 86 THROUGH 126 )
12X-RAY DIFFRACTION12CHAIN 'A' AND (RESID 127 THROUGH 151 )
13X-RAY DIFFRACTION13CHAIN 'A' AND (RESID 152 THROUGH 197 )
14X-RAY DIFFRACTION14CHAIN 'A' AND (RESID 198 THROUGH 227 )
15X-RAY DIFFRACTION15CHAIN 'A' AND (RESID 228 THROUGH 262 )
16X-RAY DIFFRACTION16CHAIN 'A' AND (RESID 263 THROUGH 276 )
17X-RAY DIFFRACTION17CHAIN 'B' AND (RESID -1 THROUGH 8 )
18X-RAY DIFFRACTION18CHAIN 'B' AND (RESID 9 THROUGH 27 )
19X-RAY DIFFRACTION19CHAIN 'B' AND (RESID 28 THROUGH 38 )
20X-RAY DIFFRACTION20CHAIN 'B' AND (RESID 39 THROUGH 46 )
21X-RAY DIFFRACTION21CHAIN 'B' AND (RESID 47 THROUGH 56 )
22X-RAY DIFFRACTION22CHAIN 'B' AND (RESID 57 THROUGH 63 )
23X-RAY DIFFRACTION23CHAIN 'B' AND (RESID 64 THROUGH 77 )
24X-RAY DIFFRACTION24CHAIN 'B' AND (RESID 78 THROUGH 90 )
25X-RAY DIFFRACTION25CHAIN 'B' AND (RESID 91 THROUGH 99 )
26X-RAY DIFFRACTION26CHAIN 'N' AND (RESID 2 THROUGH 7 )

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