Mass: 18.015 Da / Num. of mol.: 84 / Source method: isolated from a natural source / Formula: H2O
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Details
Sequence details
MET 0 in chain A, C is N-terminal extension to inhibit strand-swap dimerization. About scFv TSP7 ...MET 0 in chain A, C is N-terminal extension to inhibit strand-swap dimerization. About scFv TSP7 (chain B, D), residues 120-138(SAGGGGSGGGGSGGGGSDI) belong to the linker joining the heavy to the light chain of the antibody, and Residues 246-253(SAHHHHHH) belong to the purification tag.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.47 Å3/Da / Density % sol: 50.3 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 200 mM potassium phosphate monobasic, 20% PEG 3350
Resolution: 2.55→109.26 Å / Cor.coef. Fo:Fc: 0.93 / Cor.coef. Fo:Fc free: 0.884 / SU B: 27.018 / SU ML: 0.273 / Cross valid method: THROUGHOUT / ESU R: 0.973 / ESU R Free: 0.339 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2709
1222
5.2 %
RANDOM
Rwork
0.2141
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obs
0.21693
22077
98.76 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å