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- PDB-5jqg: An apo tubulin-RB-TTL complex structure used for side-by-side com... -

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Basic information

Entry
Database: PDB / ID: 5jqg
TitleAn apo tubulin-RB-TTL complex structure used for side-by-side comparison
Components
  • Stathmin-4
  • Tubulin alpha-1B chain
  • Tubulin beta chain
  • Uncharacterized protein
KeywordsSTRUCTURAL PROTEIN / apo structure
Function / homology
Function and homology information


tubulin-tyrosine ligase activity / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Resolution of Sister Chromatid Cohesion / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Mitotic Prometaphase / Carboxyterminal post-translational modifications of tubulin / RHOH GTPase cycle ...tubulin-tyrosine ligase activity / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Resolution of Sister Chromatid Cohesion / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Mitotic Prometaphase / Carboxyterminal post-translational modifications of tubulin / RHOH GTPase cycle / EML4 and NUDC in mitotic spindle formation / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / PKR-mediated signaling / Separation of Sister Chromatids / The role of GTSE1 in G2/M progression after G2 checkpoint / Aggrephagy / microtubule depolymerization / RHO GTPases activate IQGAPs / RHO GTPases Activate Formins / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / Recruitment of NuMA to mitotic centrosomes / COPI-mediated anterograde transport / regulation of microtubule polymerization or depolymerization / tubulin binding / spindle microtubule / protein modification process / structural constituent of cytoskeleton / microtubule cytoskeleton organization / neuron migration / neuron projection development / mitotic cell cycle / microtubule cytoskeleton / growth cone / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / neuron projection / nucleotide binding / GTPase activity / GTP binding / Golgi apparatus / metal ion binding / cytoplasm
Similarity search - Function
Methane Monooxygenase Hydroxylase; Chain G, domain 1 - #30 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Rossmann fold - #11480 / Helix hairpin bin / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily ...Methane Monooxygenase Hydroxylase; Chain G, domain 1 - #30 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Rossmann fold - #11480 / Helix hairpin bin / Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Tubulin/FtsZ, C-terminal domain / Tubulin/FtsZ, GTPase domain / ATP-grasp fold, B domain / D-amino Acid Aminotransferase; Chain A, domain 1 / 60s Ribosomal Protein L30; Chain: A; / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Helix non-globular / Special / Helix Hairpins / Rossmann fold / 2-Layer Sandwich / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Tubulin tyrosine ligase / Tubulin beta chain / Stathmin-4 / Tubulin alpha-1B chain
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Gallus gallus (chicken)
Sus scrofa (pig)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.24 Å
AuthorsWang, Y.X. / Naismith, J.H. / Zhu, X.
CitationJournal: Nat Commun / Year: 2016
Title: Pironetin reacts covalently with cysteine-316 of alpha-tubulin to destabilize microtubule
Authors: Yang, J. / Wang, Y.X. / Wang, T. / Jiang, J. / Botting, C.H. / Liu, H. / Chen, Q. / Yang, J. / Naismith, J.H. / Zhu, X. / Chen, L.
History
DepositionMay 4, 2016Deposition site: RCSB / Processing site: PDBJ
Revision 1.0May 18, 2016Provider: repository / Type: Initial release
Revision 1.1Sep 27, 2017Group: Data collection / Database references / Derived calculations
Category: citation / citation_author ...citation / citation_author / diffrn_detector / pdbx_struct_oper_list
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _diffrn_detector.detector / _pdbx_struct_oper_list.symmetry_operation
Revision 1.2Nov 8, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_atom_id / _pdbx_struct_conn_angle.ptnr2_label_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tubulin alpha-1B chain
B: Tubulin beta chain
C: Tubulin alpha-1B chain
D: Tubulin beta chain
E: Stathmin-4
F: Uncharacterized protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)264,60821
Polymers261,4476
Non-polymers3,16115
Water5,999333
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area22750 Å2
ΔGint-127 kcal/mol
Surface area79520 Å2
Unit cell
Length a, b, c (Å)105.050, 158.120, 180.630
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11A
21C
12B
22D

NCS domain segments:

Component-ID: _ / Refine code: _

Dom-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11METMETGLYGLYAA1 - 4361 - 436
21METMETGLYGLYCC1 - 4361 - 436
12ARGARGALAALABB2 - 4282 - 428
22ARGARGALAALADD2 - 4282 - 428

NCS ensembles :
ID
1
2

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Components

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Protein , 4 types, 6 molecules ACBDEF

#1: Protein Tubulin alpha-1B chain / Alpha-tubulin ubiquitous / Tubulin K-alpha-1 / Tubulin alpha-ubiquitous chain


Mass: 50204.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: Q2XVP4
#2: Protein Tubulin beta chain / Beta-tubulin


Mass: 49907.770 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: P02554
#3: Protein Stathmin-4 / Stathmin-like protein B3 / RB3


Mass: 16844.162 Da / Num. of mol.: 1 / Fragment: UNP residues 49-189
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Stmn4 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P63043
#4: Protein Uncharacterized protein / tubulin tyrosine ligase


Mass: 44378.496 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: TTL / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: E1BQ43

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Non-polymers , 8 types, 348 molecules

#5: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#7: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Ca
#8: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#9: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#10: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#11: Chemical ChemComp-ACP / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / ADENOSINE-5'-[BETA, GAMMA-METHYLENE]TRIPHOSPHATE


Mass: 505.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C11H18N5O12P3 / Comment: AMP-PCP, energy-carrying molecule analogue*YM
#12: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 333 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.87 Å3/Da / Density % sol: 57.13 %
Crystal growTemperature: 293 K / Method: vapor diffusion / pH: 6.7
Details: 0.1M MES, 6% PEG4000, 5% Glycerol, 30mM CaCl2, 30mM MgCl2

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97853 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 8, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97853 Å / Relative weight: 1
ReflectionResolution: 2.24→35.22 Å / Num. obs: 144729 / % possible obs: 100 % / Redundancy: 6.7 % / Net I/σ(I): 14.9
Reflection shellResolution: 2.24→2.3 Å

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Processing

Software
NameVersionClassification
REFMAC5.8.0135refinement
xia2data reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4I55

4i55


Resolution: 2.24→35.22 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.941 / SU B: 9.577 / SU ML: 0.12 / Cross valid method: THROUGHOUT / ESU R: 0.212 / ESU R Free: 0.174 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.21247 7246 5 %RANDOM
Rwork0.18081 ---
obs0.18238 137397 99.93 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.06 Å
Displacement parametersBiso mean: 49.326 Å2
Baniso -1Baniso -2Baniso -3
1-1.76 Å20 Å20 Å2
2--0.14 Å2-0 Å2
3----1.91 Å2
Refinement stepCycle: LAST / Resolution: 2.24→35.22 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16999 0 187 333 17519
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0150.01917731
X-RAY DIFFRACTIONr_bond_other_d0.0060.0216473
X-RAY DIFFRACTIONr_angle_refined_deg1.691.96224041
X-RAY DIFFRACTIONr_angle_other_deg1.299337952
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.6452175
X-RAY DIFFRACTIONr_dihedral_angle_2_deg35.72924.327855
X-RAY DIFFRACTIONr_dihedral_angle_3_deg13.642152980
X-RAY DIFFRACTIONr_dihedral_angle_4_deg16.65615107
X-RAY DIFFRACTIONr_chiral_restr0.0970.22621
X-RAY DIFFRACTIONr_gen_planes_refined0.0090.02120114
X-RAY DIFFRACTIONr_gen_planes_other0.0050.024142
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it2.2632.148658
X-RAY DIFFRACTIONr_mcbond_other2.2612.148657
X-RAY DIFFRACTIONr_mcangle_it3.1373.19710815
X-RAY DIFFRACTIONr_mcangle_other3.1373.19810816
X-RAY DIFFRACTIONr_scbond_it3.5732.5599073
X-RAY DIFFRACTIONr_scbond_other3.5732.569074
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other5.153.68913216
X-RAY DIFFRACTIONr_long_range_B_refined6.89517.66719207
X-RAY DIFFRACTIONr_long_range_B_other6.89917.5819122
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05

Ens-IDDom-IDAuth asym-IDNumberRms dev position (Å)
11A495600.13
12C495600.13
21B501000.08
22D501000.08
LS refinement shellResolution: 2.24→2.298 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.284 504 -
Rwork0.24 10048 -
obs--99.96 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.69340.1496-0.33713.57490.94991.41540.0040.13340.2437-0.36170.11350.0213-0.74140.2199-0.11750.5481-0.14390.07640.1669-0.06970.197328.51397.86754.659
21.2698-1.10840.50249.0241-0.10241.9294-0.09170.30050.2434-0.71450.2785-0.0242-0.66920.1427-0.18680.3364-0.11660.09280.254-0.06920.247429.32983.68843.713
31.03270.115-0.37772.13151.16031.92080.01710.0581-0.01360.0540.1697-0.1418-0.17730.2365-0.18680.1672-0.03010.0350.1039-0.10530.191527.69880.99559.848
41.4971-0.4704-0.64363.51930.89172.05860.0695-0.02070.02510.2554-0.05610.2847-0.0961-0.229-0.01340.2489-0.00960.08820.1078-0.0730.213512.67286.45875.458
50.5964-0.2675-0.26172.83181.78652.5026-0.0497-0.0992-0.020.59760.0920.05450.29880.14-0.04230.1922-0.02480.02930.1081-0.07630.204823.06274.09968.672
62.276-1.2009-0.44963.29680.76171.61120.13750.29040.3503-0.4507-0.09950.0693-0.6268-0.0622-0.0380.27950.00940.01890.1883-0.03490.249720.34365.11517.947
71.14380.00670.05512.47581.72932.49190.002-0.08930.12570.0399-0.12630.2342-0.1175-0.1650.12430.047900.0240.1217-0.09280.187517.34955.06428.189
82.0756-0.49220.27741.76170.90182.5377-0.1297-0.0669-0.00210.1762-0.15940.35730.0286-0.65690.28910.1372-0.02350.06620.2849-0.16340.30897.28358.72243.985
90.8267-0.35450.0331.07811.44143.2827-0.1735-0.0249-0.01210.2162-0.08460.26560.1134-0.31730.25810.0816-0.03760.07470.2599-0.08080.355510.06154.05535.284
100.3351-0.03630.7392.58232.00683.4064-0.00110.0158-0.01210.57570.0313-0.02930.48090.2123-0.03020.185-0.0067-0.00750.2885-0.03820.243624.49236.28930.206
112.0761-0.6498-0.19913.3881-0.38251.3128-0.09270.13980.1866-0.26190.04760.1273-0.24080.04190.04510.1351-0.0545-0.02180.10080.03130.151514.7440.746-14.691
121.16230.0965-0.10551.77570.43151.3426-0.05670.16810.0456-0.15790.1202-0.0058-0.05760.1008-0.06350.0662-0.02280.01320.0671-0.01120.115118.27227.262-9.522
134.0801-1.50692.30260.9428-0.47722.64410.03540.04550.2201-0.0551-0.07230.0529-0.108-0.16780.03690.0388-0.01070.02780.073-0.0120.14456.88832.9734.139
147.2866-4.1434-0.38844.53771.2670.71540.04260.1895-0.2778-0.0126-0.20230.45620.0806-0.17890.15960.0943-0.11020.03440.2435-0.00990.2375-1.77325.7646.676
151.0264-0.50680.03451.65580.90931.5103-0.0682-0.07190.02010.173-0.00610.12550.1709-0.12140.07430.0708-0.04420.0310.0537-0.02350.162210.49123.6635.34
163.5021-1.1510.29423.12770.17772.6169-0.06370.6590.105-0.7670.05370.0179-0.2183-0.07930.01010.4737-0.08710.03780.4295-0.06470.174618.3559.3-44.787
171.94080.0025-0.31412.03070.52561.4511-0.11010.3385-0.1789-0.4250.1524-0.15520.26250.074-0.04230.4316-0.04520.07720.3084-0.17530.192322.686-3.225-35.75
182.1694-0.4289-0.151.36970.66461.8552-0.07850.2844-0.3917-0.2105-0.07050.2920.2933-0.36390.14890.2801-0.06240.00450.2346-0.13820.270610.9920.148-22.994
192.2158-0.3086-0.59871.64560.34692.0415-0.18210.3173-0.2518-0.22060.06020.16610.3103-0.15220.12190.3005-0.08150.0190.1728-0.10920.22459.876-4.044-22.134
204.3944-1.0697-4.12531.38111.87677.3681-0.08530.0433-0.3686-0.010.0864-0.23560.53830.3798-0.0010.41550.08680.06770.1927-0.16710.39329.679-15.419-23.695
212.76180.1270.336510.688-6.93319.90740.1226-0.32940.11460.6017-0.00740.1265-0.17630.3653-0.11520.3403-0.0599-0.0020.2187-0.12730.220124.2399.36382.955
221.7502-1.4641-1.84653.81993.75673.8613-0.3597-0.3568-0.03310.95010.9118-0.61990.82780.9237-0.55210.64770.2556-0.02590.6633-0.25240.488338.35878.36868.711
230.67471.54552.1664.69416.874110.1410.0240.00610.04240.17830.1349-0.11410.28240.2458-0.15890.18220.0148-0.00990.2981-0.10450.320841.54951.5230.97
240.1895-1.0857-1.008614.68712.641511.40360.0404-0.0221-0.09640.15650.1081-0.3020.31940.2596-0.14850.19520.01810.06130.3221-0.13350.336540.68812.958-13.343
2511.23280.1131-0.33540.5209-2.501718.7243-0.1346-0.1278-0.2208-0.06210.057-0.0110.03360.28640.07770.31770.06330.08630.3173-0.12140.374848.121-16.265-41.726
261.79910.4898-1.39922.4744-0.41634.6153-0.30910.2442-0.5604-0.23120.0133-0.02221.0389-0.26260.29580.5115-0.07190.1120.1384-0.11590.36896.43954.56270.737
272.04431.5789-0.59833.5368-1.28985.0698-0.0343-0.5774-0.330.3574-0.317-0.6779-0.03731.18320.35130.4350.0364-0.0640.56050.16220.512614.49360.845102.242
282.10331.1647-2.29292.0887-1.42244.2685-0.3441-0.3594-0.76150.1931-0.0498-0.33050.83020.34360.39390.60650.0650.08460.16090.13440.5419-0.46948.567102.112
291.72170.4598-2.79341.3486-0.67715.228-0.19940.1313-0.27560.10050.14460.03450.379-0.24620.05480.31760.00480.03680.10660.02630.3375-1.6259.33289.162
301.7323-2.01980.112112.9950.98130.12360.11680.61340.1292-0.0857-0.1288-0.14780.02020.09950.01210.4017-0.00850.03510.32820.00510.3538-8.11676.38794.281
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A1 - 65
2X-RAY DIFFRACTION2A66 - 107
3X-RAY DIFFRACTION3A108 - 279
4X-RAY DIFFRACTION4A280 - 331
5X-RAY DIFFRACTION5A332 - 437
6X-RAY DIFFRACTION6B2 - 125
7X-RAY DIFFRACTION7B126 - 254
8X-RAY DIFFRACTION8B255 - 351
9X-RAY DIFFRACTION9B352 - 387
10X-RAY DIFFRACTION10B388 - 428
11X-RAY DIFFRACTION11C1 - 81
12X-RAY DIFFRACTION12C82 - 244
13X-RAY DIFFRACTION13C245 - 283
14X-RAY DIFFRACTION14C284 - 311
15X-RAY DIFFRACTION15C312 - 440
16X-RAY DIFFRACTION16D2 - 93
17X-RAY DIFFRACTION17D94 - 248
18X-RAY DIFFRACTION18D249 - 297
19X-RAY DIFFRACTION19D298 - 394
20X-RAY DIFFRACTION20D395 - 431
21X-RAY DIFFRACTION21E6 - 18
22X-RAY DIFFRACTION22E19 - 55
23X-RAY DIFFRACTION23E56 - 85
24X-RAY DIFFRACTION24E86 - 134
25X-RAY DIFFRACTION25E135 - 141
26X-RAY DIFFRACTION26F1 - 69
27X-RAY DIFFRACTION27F70 - 180
28X-RAY DIFFRACTION28F181 - 277
29X-RAY DIFFRACTION29F278 - 378
30X-RAY DIFFRACTION30F379 - 384

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