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Structure paper

TitlePironetin Reacts Covalently with Cysteine-316 of Alpha-Tubulin to Destabilize Microtubule.
Journal, issue, pagesNat. Commun., Vol. 7, Page 12103-, Year 2016
Publish dateNov 16, 2015 (structure data deposition date)
AuthorsYang, J. / Wang, Y. / Wang, T. / Jiang, J. / Botting, C.H. / Liu, H. / Chen, Q. / Naismith, J.H. / Zhu, X. / Chen, L.
External linksNat. Commun. / PubMed:27357539
MethodsX-ray diffraction
Resolution2.24 - 2.61 Å
Structure data

PDB-5fnv:
a new complex structure of tubulin with an alpha-beta unsaturated lactone
Method: X-RAY DIFFRACTION / Resolution: 2.61 Å

PDB-5jqg:
An apo tubulin-RB-TTL complex structure used for side-by-side comparison
Method: X-RAY DIFFRACTION / Resolution: 2.24 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-X3H:
PIRONETIN

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

Source
  • gallus gallus (chicken)
  • rattus norvegicus (Norway rat)
  • sus scrofa (pig)
KeywordsSTRUCTURAL PROTEIN / TUBULIN COMPLEX ALPHA-BETA UNSATURATED LACTONE / apo structure

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