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- PDB-5jlw: AntpHD with 15bp DNA duplex R-monothioated at Cytidine-8 -

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Basic information

Entry
Database: PDB / ID: 5jlw
TitleAntpHD with 15bp DNA duplex R-monothioated at Cytidine-8
Components
  • DNA (5'-D(*AP*GP*AP*AP*AP*GP*CP*(C7R)P*AP*TP*TP*AP*GP*AP*G)-3')
  • DNA (5'-D(*TP*CP*TP*CP*TP*AP*AP*TP*GP*GP*CP*TP*TP*TP*C)-3')
  • Homeotic protein antennapedia
KeywordsTranscription Regulator/DNA / HOMEODOMAIN / DNA-BINDING PROTEIN / COMPLEX (HOMEODOMAIN-DNA) / TRANSCRIPTION-DNA COMPLEX / Transcription Regulator-DNA complex / monothiolated DNA
Function / homology
Function and homology information


specification of segmental identity, antennal segment / specification of segmental identity, thorax / neuroblast development / muscle cell fate specification / lymph gland development / ventral cord development / anterior/posterior axis specification / anterior/posterior pattern specification / midgut development / regulation of neurogenesis ...specification of segmental identity, antennal segment / specification of segmental identity, thorax / neuroblast development / muscle cell fate specification / lymph gland development / ventral cord development / anterior/posterior axis specification / anterior/posterior pattern specification / midgut development / regulation of neurogenesis / heart development / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleus / cytoplasm
Similarity search - Function
Homeobox protein, antennapedia type / Homeobox protein, antennapedia type, conserved site / 'Homeobox' antennapedia-type protein signature. / Homeobox domain, metazoa / Homeobox, conserved site / 'Homeobox' domain signature. / Homeodomain / 'Homeobox' domain profile. / Homeodomain / Homeobox domain ...Homeobox protein, antennapedia type / Homeobox protein, antennapedia type, conserved site / 'Homeobox' antennapedia-type protein signature. / Homeobox domain, metazoa / Homeobox, conserved site / 'Homeobox' domain signature. / Homeodomain / 'Homeobox' domain profile. / Homeodomain / Homeobox domain / Homeodomain-like / Homeobox-like domain superfamily / Arc Repressor Mutant, subunit A / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
NICKEL (II) ION / DNA / DNA (> 10) / Homeotic protein antennapedia
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
unidentified (others)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.088 Å
AuthorsWhite, M.A. / Zandarashvili, L. / Iwahara, J. / Nguyen, D.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM105931 United States
CitationJournal: Chembiochem / Year: 2016
Title: Stereospecific Effects of Oxygen-to-Sulfur Substitution in DNA Phosphate on Ion Pair Dynamics and Protein-DNA Affinity.
Authors: Nguyen, D. / Zandarashvili, L. / White, M.A. / Iwahara, J.
History
DepositionApr 27, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 22, 2016Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2016Group: Structure summary
Revision 1.2Sep 14, 2016Group: Database references
Revision 1.3Sep 20, 2017Group: Author supporting evidence / Derived calculations / Category: pdbx_audit_support / pdbx_struct_oper_list
Item: _pdbx_audit_support.funding_organization / _pdbx_struct_oper_list.symmetry_operation
Revision 1.4Sep 27, 2017Group: Data collection / Category: diffrn_detector / Item: _diffrn_detector.detector
Revision 1.5Mar 7, 2018Group: Data collection / Category: diffrn_source / Item: _diffrn_source.source
Revision 1.6Dec 25, 2019Group: Author supporting evidence / Database references / Category: pdbx_audit_support / struct_ref_seq
Item: _pdbx_audit_support.funding_organization / _struct_ref_seq.db_align_beg / _struct_ref_seq.db_align_end
Revision 1.7Sep 27, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / diffrn_detector / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_conn_type
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _diffrn_detector.detector / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr1_symmetry / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry / _struct_conn_type.id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Homeotic protein antennapedia
B: DNA (5'-D(*AP*GP*AP*AP*AP*GP*CP*(C7R)P*AP*TP*TP*AP*GP*AP*G)-3')
C: DNA (5'-D(*TP*CP*TP*CP*TP*AP*AP*TP*GP*GP*CP*TP*TP*TP*C)-3')
D: Homeotic protein antennapedia
E: DNA (5'-D(*AP*GP*AP*AP*AP*GP*CP*(C7R)P*AP*TP*TP*AP*GP*AP*G)-3')
F: DNA (5'-D(*TP*CP*TP*CP*TP*AP*AP*TP*GP*GP*CP*TP*TP*TP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)35,01515
Polymers34,3096
Non-polymers7079
Water3,819212
1
A: Homeotic protein antennapedia
B: DNA (5'-D(*AP*GP*AP*AP*AP*GP*CP*(C7R)P*AP*TP*TP*AP*GP*AP*G)-3')
C: DNA (5'-D(*TP*CP*TP*CP*TP*AP*AP*TP*GP*GP*CP*TP*TP*TP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)17,3897
Polymers17,1543
Non-polymers2354
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3500 Å2
ΔGint-41 kcal/mol
Surface area8720 Å2
MethodPISA
2
D: Homeotic protein antennapedia
E: DNA (5'-D(*AP*GP*AP*AP*AP*GP*CP*(C7R)P*AP*TP*TP*AP*GP*AP*G)-3')
F: DNA (5'-D(*TP*CP*TP*CP*TP*AP*AP*TP*GP*GP*CP*TP*TP*TP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)17,6268
Polymers17,1543
Non-polymers4725
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3340 Å2
ΔGint-34 kcal/mol
Surface area8760 Å2
MethodPISA
Unit cell
Length a, b, c (Å)61.266, 75.888, 93.696
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number17
Space group name H-MP2221
DetailsThere are two protein:DNA complexes in the a.u.

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Components

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Protein , 1 types, 2 molecules AD

#1: Protein Homeotic protein antennapedia


Mass: 7961.297 Da / Num. of mol.: 2 / Mutation: C335S
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: Antp, CG1028 / Production host: Escherichia coli (E. coli) / References: UniProt: P02833

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DNA chain , 2 types, 4 molecules BECF

#2: DNA chain DNA (5'-D(*AP*GP*AP*AP*AP*GP*CP*(C7R)P*AP*TP*TP*AP*GP*AP*G)-3')


Mass: 4667.128 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: R-Monothiolated phosphate at Cytidine 8 / Source: (synth.) unidentified (others)
#3: DNA chain DNA (5'-D(*TP*CP*TP*CP*TP*AP*AP*TP*GP*GP*CP*TP*TP*TP*C)-3')


Mass: 4525.944 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) unidentified (others)

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Non-polymers , 3 types, 221 molecules

#4: Chemical
ChemComp-NI / NICKEL (II) ION


Mass: 58.693 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Ni
#5: Chemical ChemComp-MPD / (4S)-2-METHYL-2,4-PENTANEDIOL


Mass: 118.174 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C6H14O2 / Comment: precipitant*YM
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 212 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.17 Å3/Da / Density % sol: 61.25 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 20mM bis-Tris propane, 10mM NiCl2, 4-6% 2-methyl-2,4-pentanediol
PH range: 6.0 - 7.5

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Data collection

DiffractionMean temperature: 100 K / Ambient temp details: Cryostream
Diffraction sourceSource: SEALED TUBE / Type: BRUKER D8 QUEST / Wavelength: 1.5418 Å
DetectorType: BRUKER PHOTON 100 / Detector: CMOS / Date: Dec 9, 2015 / Details: Helios
RadiationMonochromator: Multilayer / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 2.088→19.3 Å / Num. obs: 48110 / % possible obs: 97.5 % / Redundancy: 5.72 % / Rmerge(I) obs: 0.0791 / Rsym value: 0.0499 / Net I/σ(I): 11.99
Reflection shellResolution: 2.09→2.19 Å / Redundancy: 4.28 % / Rmerge(I) obs: 0.5032 / Mean I/σ(I) obs: 1.84 / % possible all: 92.1

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Processing

Software
NameVersionClassification
PHENIX1.10_2155refinement
SAINTdata reduction
PROTEUM PLUSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4XID

4xid


Resolution: 2.088→19.298 Å / SU ML: 0.29 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.32
RfactorNum. reflection% reflection
Rfree0.2424 2390 4.97 %
Rwork0.2181 --
obs0.2193 48110 96.05 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.088→19.298 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1037 1218 30 212 2497
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0092479
X-RAY DIFFRACTIONf_angle_d1.1173595
X-RAY DIFFRACTIONf_dihedral_angle_d27.4541265
X-RAY DIFFRACTIONf_chiral_restr0.049383
X-RAY DIFFRACTIONf_plane_restr0.007248
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.0881-2.13070.39141110.40132085X-RAY DIFFRACTION75
2.1307-2.1770.35111420.32722691X-RAY DIFFRACTION96
2.177-2.22760.34011440.31122728X-RAY DIFFRACTION97
2.2276-2.28320.28751480.31052758X-RAY DIFFRACTION98
2.2832-2.34480.34551430.29042743X-RAY DIFFRACTION98
2.3448-2.41370.28491450.27762765X-RAY DIFFRACTION99
2.4137-2.49140.37121400.28732729X-RAY DIFFRACTION98
2.4914-2.58030.29161460.27112723X-RAY DIFFRACTION98
2.5803-2.68330.28741430.27542772X-RAY DIFFRACTION98
2.6833-2.80510.34591420.25562764X-RAY DIFFRACTION98
2.8051-2.95250.25471410.25012759X-RAY DIFFRACTION99
2.9525-3.13680.21961440.23092762X-RAY DIFFRACTION98
3.1368-3.37780.2541400.18932670X-RAY DIFFRACTION95
3.3778-3.71550.18491420.17292716X-RAY DIFFRACTION98
3.7155-4.24810.19561500.16612750X-RAY DIFFRACTION99
4.2481-5.33340.20121460.16722775X-RAY DIFFRACTION99
5.3334-19.29840.20631230.19742530X-RAY DIFFRACTION89
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.0496-0.03970.00750.0448-0.02220.01840.15820.26230.13540.19840.1272-0.16240.07910.14570.00110.45730.07990.00370.4652-0.00860.33624.512714.20772.2277
20.01230.0145-0.00530.0157-0.01070.01820.2069-0.081-0.02670.0920.08510.08570.03180.04690.00060.33480.07520.02210.34170.00260.330217.259414.53627.9079
30.00560.0127-0.00650.0293-0.01550.01410.0803-0.00710.215-0.0494-0.0060.13440.26-0.0501-0.00010.39670.1307-0.00030.41960.02490.34879.524814.92188.4647
40.0177-0.0012-0.01740.01480.00150.01470.0079-0.14230.05480.06840.19460.16790.0724-0.13230.00050.38650.1128-0.09960.4398-0.05250.43245.727121.10372.8856
50.0360.0080.01840.01680.01190.01640.04310.05760.3126-0.09030.11550.2068-0.0268-0.0923-0.00070.58080.0606-0.07520.3685-0.0030.47312.429729.39374.837
60.03250.0043-0.00190.0025-0.0030.0130.0978-0.15610.08180.0434-0.01780.0050.04650.0008-0.00050.28530.0485-0.00020.387-0.00580.401418.811826.2088.1274
70.0178-0.0056-0.01260.0250.01330.0142-0.08250.06990.115-0.2164-0.1068-0.1041-0.10180.1311-0.00090.43680.1320.02650.48180.08720.350620.662224.41670.5328
80.0141-0.0059-0.00640.00950.01320.030.09990.0915-0.0167-0.2433-0.06250.1602-0.1857-0.02170.00010.36010.0779-0.05080.33330.04250.281614.365119.0645-2.4554
90.0037-0.0098-0.00630.02060.0110.01130.20790.1872-0.10250.1173-0.00620.10160.0706-0.08120.00010.46770.0096-0.17060.3049-0.0070.43128.816712.6528-3.0156
100.00360.00350.00290.0122-0.00370.0052-0.1025-0.0175-0.08830.02390.0138-0.02940.0604-0.144700.7798-0.1291-0.0380.68430.03410.97374.1267.6904-2.5273
110.03950.0490.02510.05880.01540.01990.05360.4066-0.04160.223-0.31260.045-0.1630.35690.00020.5190.1482-0.08030.41690.1150.49316.51834.7578-11.2167
120.87140.6051-0.85211.2149-0.57830.83780.44520.1762-0.3019-0.113-0.00930.32550.258-0.05130.44340.6510.229-0.22670.4965-0.13530.407510.366315.6961-13.4554
130.00750.01480.00320.0273-0.00280.02390.34580.3006-0.02840.278-0.29660.1384-0.40570.2197-0.00040.67630.20850.03460.49170.060.399327.35158.7437-9.7201
140.0781-0.01790.00710.0172-0.00890.04610.17060.35360.02260.061-0.04010.04640.1723-0.2098-00.50340.1552-0.05970.4363-0.06990.370521.59245.8472-8.4403
150.0545-0.12970.01780.4064-0.17240.1311-0.2310.36290.4136-0.234-0.13230.0138-0.1920.1081-0.41020.91250.9671-0.19610.92840.46650.520513.846821.6942-17.2384
160.02780.0252-0.01630.0307-0.02460.01990.50240.128-0.07620.1135-0.42940.00420.1348-0.1076-00.69970.1393-0.17080.4326-0.03570.46760.879827.9153-11.3182
170.0098-0.00980.00860.0160.00370.0110.1096-0.00140.20760.049-0.0382-0.0425-0.2602-0.1766-00.75440.0573-0.00640.4583-0.0890.478915.467130.529428.1017
180.0069-0.00460.00340.0052-0.00070.00520.05550.00380.0025-0.1912-0.1320.0888-0.16670.00660.00020.40940.105-0.0350.3222-0.01590.277812.831226.883717.8332
190.0162-0.0055-0.00480.0069-0.00580.01420.04080.0075-0.0395-0.25120.0047-0.00620.062-0.20510.00030.31380.11070.01120.3624-0.02340.298211.992718.032218.9632
200.00590.0096-0.0020.0138-0.00640.0140.18770.0292-0.12160.05720.00410.08090.0974-0.05740.00040.37850.0555-0.01290.3901-0.09830.421810.182311.143619.2918
210.0831-0.0015-0.02080.01420.0180.02120.1499-0.12310.0896-0.16140.04020.09360.1230.09130.0010.43790.1070.02460.3937-0.00220.34920.218210.389924.9197
220.0035-0.00220.00140.010.00320.0025-0.02770.0137-0.0558-0.10410.02040.01840.01130.05150.00010.29220.14470.05540.53420.08520.303124.270513.234320.0024
230.0217-0.00490.0010.00410.00450.0061-0.09940.0388-0.0827-0.0711-0.030.017-0.05210.0854-00.32490.0321-0.01640.40150.04270.3423.852821.939118.9721
240.02160.0205-0.00060.02830.00480.0080.0148-0.1563-0.1903-0.186-0.0249-0.1465-0.18740.16680.00060.4244-0.0940.0450.5524-0.00760.348523.77622.746625.0139
250.02040.0082-0.03220.0344-0.00780.047-0.08040.07010.0715-0.01220.07820.09610.04360.048-0.00010.18870.0764-0.00790.3241-0.00040.243415.866918.288329.6494
260.0039-0.00470.00130.01770.0030.0162-0.06810.03260.0720.1725-0.060.09350.0444-0.0426-0.00030.2670.02830.03790.44280.04170.40966.650813.880829.6776
270.0079-0.01360.02250.0255-0.04290.07170.0095-0.01670.01710.0032-0.08750.00650.0197-0.0688-0.00450.2811-0.07870.09780.8853-0.11320.55440.30913.532230.4444
280.03480.0196-0.00010.0035-0.00910.0548-0.18440.2695-0.17180.1518-0.03280.0639-0.2720.09380.00040.29890.0655-0.04160.43810.05690.300124.66657.899937.3251
290.1267-0.01580.13350.3246-0.11590.1734-0.0810.0225-0.02950.2234-0.04010.1128-0.068-0.5102-0.18730.5880.25160.34910.62930.05310.55259.211718.781142.4996
300.03130.017-0.00830.03910.00370.00280.09490.34660.1460.34830.00920.0268-0.33920.29310.00040.63690.24620.00710.65250.08430.57588.785133.880836.2469
310.0357-0.01140.00960.0077-0.00630.01060.07260.0227-0.22420.1985-0.19010.00660.1907-0.2274-0.00040.4750.06820.09180.4832-0.13140.9172-1.124630.966636.5066
320.02780.0187-0.02920.0468-0.02940.0305-0.23760.14160.10120.3150.12770.0693-0.25330.0328-0.00010.42350.1583-0.02210.4369-0.08550.364213.477324.86534.5979
331.5212-1.1208-1.04211.44420.90910.7864-0.39360.2932-0.40050.4314-0.15240.1507-0.307-0.3038-0.09060.58640.1234-0.04360.59250.02440.541419.47528.474641.414
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 1:13)
2X-RAY DIFFRACTION2(chain A and resid 14:17)
3X-RAY DIFFRACTION3(chain A and resid 18:23)
4X-RAY DIFFRACTION4(chain A and resid 24:28)
5X-RAY DIFFRACTION5(chain A and resid 29:33)
6X-RAY DIFFRACTION6(chain A and resid 34:38)
7X-RAY DIFFRACTION7(chain A and resid 39:44)
8X-RAY DIFFRACTION8(chain A and resid 45:52)
9X-RAY DIFFRACTION9(chain A and resid 53:56)
10X-RAY DIFFRACTION10(chain A and resid 57:61)
11X-RAY DIFFRACTION11(chain B and resid 1:5)
12X-RAY DIFFRACTION12(chain B and resid 6:11)
13X-RAY DIFFRACTION13(chain B and resid 12:15)
14X-RAY DIFFRACTION14(chain C and resid 1:7)
15X-RAY DIFFRACTION15(chain C and resid 8:11)
16X-RAY DIFFRACTION16(chain C and resid 12:15)
17X-RAY DIFFRACTION17(chain D and resid 1:10)
18X-RAY DIFFRACTION18(chain D and resid 11:15)
19X-RAY DIFFRACTION19(chain D and resid 16:21)
20X-RAY DIFFRACTION20(chain D and resid 22:25)
21X-RAY DIFFRACTION21(chain D and resid 26:29)
22X-RAY DIFFRACTION22(chain D and resid 30:34)
23X-RAY DIFFRACTION23(chain D and resid 35:39)
24X-RAY DIFFRACTION24(chain D and resid 40:43)
25X-RAY DIFFRACTION25(chain D and resid 44:53)
26X-RAY DIFFRACTION26(chain D and resid 54:57)
27X-RAY DIFFRACTION27(chain D and resid 58:61)
28X-RAY DIFFRACTION28(chain E and resid 1:7)
29X-RAY DIFFRACTION29(chain E and resid 8:11)
30X-RAY DIFFRACTION30(chain E and resid 12:15)
31X-RAY DIFFRACTION31(chain F and resid 1:4)
32X-RAY DIFFRACTION32(chain F and resid 5:8)
33X-RAY DIFFRACTION33(chain F and resid 9:15)

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