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Yorodumi- PDB-5irf: Reverse transcriptase domain of group II intron maturase from Ros... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5irf | ||||||
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Title | Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P1 space group | ||||||
Components | Retron-type reverse transcriptase | ||||||
Keywords | RNA BINDING PROTEIN / Group II intron / maturase / reverse transcriptase | ||||||
Function / homology | Function and homology information RNA-directed DNA polymerase activity / defense response to virus / RNA binding Similarity search - Function | ||||||
Biological species | Roseburia intestinalis XB6B4 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å | ||||||
Authors | Zhao, C. / Pyle, A.M. | ||||||
Funding support | United States, 1items
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Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2016 Title: Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution. Authors: Zhao, C. / Pyle, A.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5irf.cif.gz | 349.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5irf.ent.gz | 289.5 KB | Display | PDB format |
PDBx/mmJSON format | 5irf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ir/5irf ftp://data.pdbj.org/pub/pdb/validation_reports/ir/5irf | HTTPS FTP |
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-Related structure data
Related structure data | 5hhjSC 5hhkC 5hhlC 5irgC C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 34918.109 Da / Num. of mol.: 4 / Fragment: UNP residues 1-305 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Roseburia intestinalis XB6B4 (bacteria) Gene: RO1_37670 / Production host: Escherichia coli (E. coli) / References: UniProt: D4L313 #2: Chemical | ChemComp-K / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.76 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 100 mM Bis-Tris propane pH 8.5, 200 mM NaOAc-3H2O, 20% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97918 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 15, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→46.47 Å / Num. obs: 152938 / % possible obs: 96.1 % / Redundancy: 3.9 % / Net I/σ(I): 19.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5HHJ Resolution: 1.6→46.47 Å / SU ML: 0.26 / Cross valid method: FREE R-VALUE / σ(F): 1.92 / Phase error: 27.94 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.8 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→46.47 Å
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Refine LS restraints |
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LS refinement shell |
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