ジャーナル: Cell Rep / 年: 2017 タイトル: Cbln1 and Cbln4 Are Structurally Similar but Differ in GluD2 Binding Interactions. 著者: Chen Zhong / Jinlong Shen / Huibing Zhang / Guangyi Li / Senlin Shen / Fang Wang / Kuan Hu / Longxing Cao / Yongning He / Jianping Ding / 要旨: Unlike cerebellin 1 (Cbln1), which bridges neurexin (Nrxn) receptors and δ-type glutamate receptors in a trans-synaptic triad, Cbln4 was reported to have no or weak binding for the receptors ...Unlike cerebellin 1 (Cbln1), which bridges neurexin (Nrxn) receptors and δ-type glutamate receptors in a trans-synaptic triad, Cbln4 was reported to have no or weak binding for the receptors despite sharing ∼70% sequence identity with Cbln1. Here, we report crystal structures of the homotrimers of the C1q domain of Cbln1 and Cbln4 at 2.2 and 2.3 Å resolution, respectively. Comparison of the structures suggests that the difference between Cbln1 and Cbln4 in GluD2 binding might be because of their sequence and structural divergence in loop CD. Surprisingly, we show that Cbln4 binds to Nrxn1β and forms a stable complex with the laminin, nectin, sex-hormone binding globulin (LNS) domain of Nrxn1β. Furthermore, the negative-stain electron microscopy reconstruction of hexameric full-length Cbln1 at 13 Å resolution and that of the Cbln4/Nrxn1β complex at 19 Å resolution suggest that Nrxn1β binds to the N-terminal region of Cbln4, probably through strand β10 of the S4 insert.
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9199 Å / 相対比: 1
反射
解像度: 2.3→50 Å / Num. obs: 28464 / % possible obs: 99.8 % / 冗長度: 9.1 % / Net I/σ(I): 15.8
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.6.0117
精密化
HKL-2000
dataprocessing
HKL-2000
データスケーリング
PHENIX
位相決定
精密化
解像度: 2.3→50 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.931 / SU B: 4.699 / SU ML: 0.115 / 交差検証法: FREE R-VALUE / ESU R: 0.209 / ESU R Free: 0.178 / 詳細: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT