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- PDB-5gxq: The crystal structure of the nucleosome containing H3.6 -

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Basic information

Entry
Database: PDB / ID: 5gxq
TitleThe crystal structure of the nucleosome containing H3.6
Components
  • DNA (146-MER)
  • Histone H2A type 1-B/E
  • Histone H2B type 1-J
  • Histone H3.6
  • Histone H4
KeywordsSTRUCTURAL PROTEIN/DNA / Chromatin / Nucleosome / Histone variant / STRUCTURAL PROTEIN-DNA complex
Function / homology
Function and homology information


negative regulation of tumor necrosis factor-mediated signaling pathway / negative regulation of megakaryocyte differentiation / Packaging Of Telomere Ends / Recognition and association of DNA glycosylase with site containing an affected purine / Cleavage of the damaged purine / Deposition of new CENPA-containing nucleosomes at the centromere / DNA replication-independent chromatin assembly / Cleavage of the damaged pyrimidine / Recognition and association of DNA glycosylase with site containing an affected pyrimidine / Inhibition of DNA recombination at telomere ...negative regulation of tumor necrosis factor-mediated signaling pathway / negative regulation of megakaryocyte differentiation / Packaging Of Telomere Ends / Recognition and association of DNA glycosylase with site containing an affected purine / Cleavage of the damaged purine / Deposition of new CENPA-containing nucleosomes at the centromere / DNA replication-independent chromatin assembly / Cleavage of the damaged pyrimidine / Recognition and association of DNA glycosylase with site containing an affected pyrimidine / Inhibition of DNA recombination at telomere / Meiotic synapsis / heterochromatin assembly => GO:0031507 / telomere organization / RNA Polymerase I Promoter Opening / SUMOylation of chromatin organization proteins / DNA replication-dependent chromatin assembly / DNA methylation / HCMV Late Events / innate immune response in mucosa / SIRT1 negatively regulates rRNA expression / ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression / Condensation of Prophase Chromosomes / PRC2 methylates histones and DNA / Defective pyroptosis / RNA Polymerase I Promoter Escape / HDACs deacetylate histones / nuclear chromosome / Transcriptional regulation by small RNAs / NoRC negatively regulates rRNA expression / Nonhomologous End-Joining (NHEJ) / Formation of the beta-catenin:TCF transactivating complex / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / B-WICH complex positively regulates rRNA expression / DNA-templated transcription, initiation / Metalloprotease DUBs / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / DNA Damage/Telomere Stress Induced Senescence / lipopolysaccharide binding / G2/M DNA damage checkpoint / nucleosome assembly / RMTs methylate histone arginines / HCMV Early Events / HDMs demethylate histones / Pre-NOTCH Transcription and Translation / Meiotic recombination / PKMTs methylate histone lysines / nucleosome / Activation of anterior HOX genes in hindbrain development during early embryogenesis / UCH proteinases / Transcriptional regulation of granulopoiesis / E3 ubiquitin ligases ubiquitinate target proteins / RUNX1 regulates transcription of genes involved in differentiation of HSCs / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / Processing of DNA double-strand break ends / HATs acetylate histones / Senescence-Associated Secretory Phenotype (SASP) / chromosome, telomeric region / killing of cells of another organism / Oxidative Stress Induced Senescence / antibacterial humoral response / Estrogen-dependent gene expression / antimicrobial humoral immune response mediated by antimicrobial peptide / Ub-specific processing proteases / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / protein heterodimerization activity / Amyloid fiber formation / negative regulation of cell population proliferation / protein domain specific binding / protein-containing complex / RNA binding / DNA binding / extracellular space / extracellular exosome / membrane / extracellular region / nucleoplasm / nucleus / cytosol
Similarity search - Function
Histone, subunit A / Histone, subunit A / Histone H2B signature. / Histone H2B / Histone H2B / Histone H2A conserved site / Histone H2A signature. / C-terminus of histone H2A / Histone H2A, C-terminal domain / Histone 2A ...Histone, subunit A / Histone, subunit A / Histone H2B signature. / Histone H2B / Histone H2B / Histone H2A conserved site / Histone H2A signature. / C-terminus of histone H2A / Histone H2A, C-terminal domain / Histone 2A / Histone H2A / Histone H4 signature. / Histone H4, conserved site / Histone H4 / Histone H4 / CENP-T/Histone H4, histone fold / Centromere kinetochore component CENP-T histone fold / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF) / Histone H3 signature 1. / Histone H3 signature 2. / Histone H3 / Histone H3/CENP-A / Histone H2A/H2B/H3 / Core histone H2A/H2B/H3/H4 / Histone-fold / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
DNA (> 100) / DNA (> 10) / DNA / Histone H3 / Histone H2A type 1-B/E / Histone H2B type 1-J / Histone H4
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.85 Å
AuthorsTaguchi, H. / Xie, Y. / Horikoshi, N. / Kurumizaka, H.
Funding support Japan, 4items
OrganizationGrant numberCountry
Japan Society for the Promotion of ScienceJP25116002 Japan
Japan Society for the Promotion of ScienceJP25250023 Japan
Japan Agency for Medical Research and Development (AMED) Japan
Japan Society for the Promotion of Science16J09361 Japan
CitationJournal: Biochemistry / Year: 2017
Title: Crystal Structure and Characterization of Novel Human Histone H3 Variants, H3.6, H3.7, and H3.8
Authors: Taguchi, H. / Xie, Y. / Horikoshi, N. / Maehara, K. / Harada, A. / Nogami, J. / Sato, K. / Arimura, Y. / Osakabe, A. / Kujirai, T. / Iwasaki, T. / Semba, Y. / Tachibana, T. / Kimura, H. / ...Authors: Taguchi, H. / Xie, Y. / Horikoshi, N. / Maehara, K. / Harada, A. / Nogami, J. / Sato, K. / Arimura, Y. / Osakabe, A. / Kujirai, T. / Iwasaki, T. / Semba, Y. / Tachibana, T. / Kimura, H. / Ohkawa, Y. / Kurumizaka, H.
History
DepositionSep 19, 2016Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Apr 19, 2017Provider: repository / Type: Initial release
Revision 1.1May 10, 2017Group: Database references
Revision 1.2Oct 18, 2017Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Histone H3.6
B: Histone H4
C: Histone H2A type 1-B/E
D: Histone H2B type 1-J
E: Histone H3.6
F: Histone H4
G: Histone H2A type 1-B/E
H: Histone H2B type 1-J
I: DNA (146-MER)
J: DNA (146-MER)


Theoretical massNumber of molelcules
Total (without water)201,87210
Polymers201,87210
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area56100 Å2
ΔGint-401 kcal/mol
Surface area73210 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)106.026, 109.764, 181.400
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein Histone H3.6


Mass: 15540.074 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: H3F3AP6 / Plasmid: pH3.6 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: A0A1X8XL64*PLUS
#2: Protein Histone H4 /


Mass: 11676.703 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HIST1H4A / Plasmid: pH4 / Production host: Escherichia coli (E. coli) / Strain (production host): JM109(DE3) / References: UniProt: P62805
#3: Protein Histone H2A type 1-B/E / Histone H2A


Mass: 14447.825 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HIST1H2AB / Plasmid: pH2A / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P04908
#4: Protein Histone H2B type 1-J / Histone H2B


Mass: 14217.516 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HIST1H2BJ / Plasmid: pH2B / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P06899
#5: DNA chain DNA (146-MER)


Mass: 45053.855 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pGEM-T(Easy) / Production host: Escherichia coli (E. coli) / Strain (production host): DH5-alpha
Sequence detailsUniProt database of chain A, E does not currently exist, but Nucleotide database code is NG_022939. ...UniProt database of chain A, E does not currently exist, but Nucleotide database code is NG_022939.1 in GenBank. Three N-terminal residues, GSH are expression tags.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.61 Å3/Da / Density % sol: 52.95 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6
Details: potassium cacodylate, potassium chloride, manganese chloride

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: BL-17A / Wavelength: 0.98 Å
DetectorType: ADSC QUANTUM 270 / Detector: CCD / Date: Feb 11, 2013
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.98 Å / Relative weight: 1
ReflectionResolution: 2.85→50 Å / Num. obs: 50454 / % possible obs: 99.6 % / Redundancy: 5.9 % / Biso Wilson estimate: 59.82 Å2 / Rmerge(I) obs: 0.084 / Χ2: 2.601 / Net I/av σ(I): 34.717 / Net I/σ(I): 13.5 / Num. measured all: 297098
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsDiffraction-ID% possible all
2.85-2.955.90.4641100
2.95-3.075.90.3631100
3.07-3.215.90.2621100
3.21-3.3860.1731100
3.38-3.5960.1281100
3.59-3.8760.0941100
3.87-4.2660.071100
4.26-4.8760.0561100
4.87-6.145.90.0541100
6.14-505.40.048196.5

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Phasing

PhasingMethod: molecular replacement
Phasing MRRfactor: 31.57 / Model details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation2.84 Å40.64 Å
Translation2.84 Å40.64 Å

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Processing

Software
NameVersionClassification
HKL-20000.98.704kdata scaling
PHASER2.1.4phasing
PDB_EXTRACT3.2data extraction
PHENIX1.10.1_2155refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3AFA
Resolution: 2.85→38.129 Å / SU ML: 0.35 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.11 / Stereochemistry target values: MLHL
RfactorNum. reflection% reflection
Rfree0.2618 2534 5.07 %
Rwork0.2191 47450 -
obs0.2213 49984 99.63 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 162.05 Å2 / Biso mean: 70.4353 Å2 / Biso min: 19.5 Å2
Refinement stepCycle: final / Resolution: 2.85→38.129 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6001 5980 0 0 11981
Num. residues----1050
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0112791
X-RAY DIFFRACTIONf_angle_d1.14718527
X-RAY DIFFRACTIONf_chiral_restr0.0542104
X-RAY DIFFRACTIONf_plane_restr0.0061336
X-RAY DIFFRACTIONf_dihedral_angle_d26.1116678
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 18

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.85-2.90480.35261520.285626072759100
2.9048-2.96410.38081320.282726352767100
2.9641-3.02850.32451580.258125952753100
3.0285-3.09890.27511320.247725932725100
3.0989-3.17640.29341610.240426002761100
3.1764-3.26220.2361340.252126162750100
3.2622-3.35810.30191170.249626272744100
3.3581-3.46650.28661300.228126272757100
3.4665-3.59030.26461590.218226002759100
3.5903-3.73390.27371300.214626372767100
3.7339-3.90370.31420.215126552797100
3.9037-4.10930.25261480.207226292777100
4.1093-4.36640.22831310.199126382769100
4.3664-4.7030.24121360.201826422778100
4.703-5.17520.25881620.201826662828100
5.1752-5.92170.24551240.21726982822100
5.9217-7.45150.28051300.226627192849100
7.4515-38.13290.21471560.19792666282294

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