#1: Journal: Acta Crystallogr.,Sect.F / Year: 2015 Title: Crystallization and Crystallographic Analysis of an Arabidopsis Nuclear Proteinaceous Rnase P. Authors: Pinker, F. / Giege, P. / Sauter, C.
History
Deposition
Jan 12, 2016
Deposition site: PDBE / Processing site: PDBE
Revision 1.0
Jan 27, 2016
Provider: repository / Type: Initial release
Revision 1.1
Mar 6, 2019
Group: Data collection / Experimental preparation / Other Category: exptl_crystal_grow / pdbx_database_proc / pdbx_database_status Item: _exptl_crystal_grow.temp / _pdbx_database_status.recvd_author_approval
Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
Nonpolymer details
ZINC ION (ZN): STRUCTURAL ZINC DOMAIN
Sequence details
ADDITION OF A GLY IN SECOND POSITION AND LEHHHHHH C- TERMINAL TAG FOR AFFINITY PURIFICATION
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.9 Å3/Da / Density % sol: 57.4 % / Description: NONE
Crystal grow
Temperature: 277 K / Method: microbatch / pH: 6 Details: PRORP2 STOCK SOLUTION WAS PREPARED AT 2.5 MG/ML IN 50 MM HEPES-NA PH 7.5, 250 MM NACL, 5%(W/V) GLYCEROL, 1 MM TCEP. PRORP2 WAS CRYSTALLIZED AT 277 K IN 2 MICROLITER MICROBATCH DROPS SET UP ...Details: PRORP2 STOCK SOLUTION WAS PREPARED AT 2.5 MG/ML IN 50 MM HEPES-NA PH 7.5, 250 MM NACL, 5%(W/V) GLYCEROL, 1 MM TCEP. PRORP2 WAS CRYSTALLIZED AT 277 K IN 2 MICROLITER MICROBATCH DROPS SET UP UNDER PARAFIN OIL BY MIXING 1 VOLUME OF PRORP2 SOLUTION WITH 1 VOLUME OF CRYSTALLANT SOLUTION CONTAINING 200 MM SODIUM MALONATE PH 6, 20% (W/V) PEG 3350.
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