ソフトウェア | 名称 | バージョン | 分類 |
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PHENIX | (1.10.1_2155: ???)精密化 | XDS | | データ削減 | XDS | | データスケーリング | PHENIX | | 位相決定 | |
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精密化 | 構造決定の手法: 分子置換 開始モデル: 2j9x 解像度: 1.972→47.255 Å / SU ML: 0.25 / 交差検証法: FREE R-VALUE / σ(F): 1.35 / 位相誤差: 25.1 / 立体化学のターゲット値: ML
| Rfactor | 反射数 | %反射 |
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Rfree | 0.232 | 4057 | 5 % |
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Rwork | 0.1784 | - | - |
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obs | 0.1811 | 81129 | 96.57 % |
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL |
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精密化ステップ | サイクル: LAST / 解像度: 1.972→47.255 Å
| タンパク質 | 核酸 | リガンド | 溶媒 | 全体 |
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原子数 | 9516 | 0 | 53 | 398 | 9967 |
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拘束条件 | Refine-ID | タイプ | Dev ideal | 数 |
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X-RAY DIFFRACTION | f_bond_d0.007 | 9715 | X-RAY DIFFRACTION | f_angle_d0.851 | 13182 | X-RAY DIFFRACTION | f_dihedral_angle_d14.636 | 5845 | X-RAY DIFFRACTION | f_chiral_restr0.051 | 1517 | X-RAY DIFFRACTION | f_plane_restr0.006 | 1726 | | | | | |
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LS精密化 シェル | 解像度 (Å) | Rfactor Rfree | Num. reflection Rfree | Rfactor Rwork | Num. reflection Rwork | Refine-ID | % reflection obs (%) |
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1.9715-1.9947 | 0.3852 | 84 | 0.341 | 1601 | X-RAY DIFFRACTION | 58 | 1.9947-2.0191 | 0.3545 | 128 | 0.2894 | 2422 | X-RAY DIFFRACTION | 89 | 2.0191-2.0446 | 0.3189 | 140 | 0.264 | 2660 | X-RAY DIFFRACTION | 95 | 2.0446-2.0715 | 0.2878 | 141 | 0.2311 | 2696 | X-RAY DIFFRACTION | 99 | 2.0715-2.0999 | 0.2646 | 144 | 0.216 | 2726 | X-RAY DIFFRACTION | 99 | 2.0999-2.1299 | 0.3028 | 143 | 0.2082 | 2727 | X-RAY DIFFRACTION | 99 | 2.1299-2.1617 | 0.2846 | 143 | 0.2143 | 2715 | X-RAY DIFFRACTION | 99 | 2.1617-2.1955 | 0.2617 | 144 | 0.2097 | 2735 | X-RAY DIFFRACTION | 99 | 2.1955-2.2315 | 0.2697 | 145 | 0.2051 | 2757 | X-RAY DIFFRACTION | 99 | 2.2315-2.2699 | 0.2691 | 141 | 0.218 | 2679 | X-RAY DIFFRACTION | 99 | 2.2699-2.3112 | 0.2754 | 145 | 0.2038 | 2739 | X-RAY DIFFRACTION | 99 | 2.3112-2.3557 | 0.2774 | 143 | 0.2069 | 2725 | X-RAY DIFFRACTION | 99 | 2.3557-2.4037 | 0.2497 | 144 | 0.1998 | 2726 | X-RAY DIFFRACTION | 99 | 2.4037-2.456 | 0.2524 | 142 | 0.2049 | 2707 | X-RAY DIFFRACTION | 99 | 2.456-2.5131 | 0.2541 | 145 | 0.196 | 2746 | X-RAY DIFFRACTION | 99 | 2.5131-2.576 | 0.2891 | 141 | 0.1993 | 2693 | X-RAY DIFFRACTION | 99 | 2.576-2.6456 | 0.2383 | 143 | 0.2004 | 2719 | X-RAY DIFFRACTION | 99 | 2.6456-2.7235 | 0.2639 | 143 | 0.1983 | 2705 | X-RAY DIFFRACTION | 98 | 2.7235-2.8114 | 0.2516 | 138 | 0.2019 | 2619 | X-RAY DIFFRACTION | 96 | 2.8114-2.9118 | 0.2554 | 135 | 0.2023 | 2566 | X-RAY DIFFRACTION | 93 | 2.9118-3.0284 | 0.27 | 142 | 0.1941 | 2696 | X-RAY DIFFRACTION | 98 | 3.0284-3.1662 | 0.2381 | 144 | 0.1887 | 2738 | X-RAY DIFFRACTION | 99 | 3.1662-3.3331 | 0.2748 | 142 | 0.178 | 2699 | X-RAY DIFFRACTION | 98 | 3.3331-3.5418 | 0.1843 | 144 | 0.1681 | 2742 | X-RAY DIFFRACTION | 99 | 3.5418-3.8152 | 0.181 | 142 | 0.1538 | 2688 | X-RAY DIFFRACTION | 98 | 3.8152-4.1989 | 0.1959 | 142 | 0.1438 | 2710 | X-RAY DIFFRACTION | 98 | 4.1989-4.806 | 0.1748 | 142 | 0.1308 | 2701 | X-RAY DIFFRACTION | 97 | 4.806-6.0531 | 0.2313 | 140 | 0.1647 | 2655 | X-RAY DIFFRACTION | 96 | 6.0531-47.2684 | 0.2121 | 147 | 0.1642 | 2780 | X-RAY DIFFRACTION | 99 |
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION ID | L11 (°2) | L12 (°2) | L13 (°2) | L22 (°2) | L23 (°2) | L33 (°2) | S11 (Å °) | S12 (Å °) | S13 (Å °) | S21 (Å °) | S22 (Å °) | S23 (Å °) | S31 (Å °) | S32 (Å °) | S33 (Å °) | T11 (Å2) | T12 (Å2) | T13 (Å2) | T22 (Å2) | T23 (Å2) | T33 (Å2) | Origin x (Å) | Origin y (Å) | Origin z (Å) |
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1 | 3.9935 | -1.9349 | 0.9301 | 8.6584 | -3.0223 | 3.2431 | -0.0989 | -0.4838 | -0.3922 | 0.4395 | 0.1255 | 0.4384 | 0.1072 | -0.1597 | -0.0553 | 0.2491 | -0.019 | 0.0767 | 0.2598 | 0.0144 | 0.2593 | -3.6851 | 47.3009 | 101.2201 | 2 | 3.8949 | 1.0824 | 0.5843 | 4.4373 | -1.3553 | 3.1242 | 0.0865 | -0.2942 | -0.0805 | 0.4383 | -0.0947 | -0.0818 | -0.3105 | 0.0334 | 0.0014 | 0.2928 | 0.0227 | 0.0014 | 0.2067 | -0.0143 | 0.2285 | 1.293 | 60.1854 | 99.0374 | 3 | 2.9663 | 1.6975 | -0.8672 | 3.0439 | 0.7386 | 7.4758 | 0.0495 | -0.4143 | -0.0981 | 0.5161 | -0.2885 | -0.3608 | 0.0881 | 0.5213 | 0.2496 | 0.4178 | 0.0682 | -0.1676 | 0.3931 | 0.0619 | 0.3581 | 14.1809 | 55.1899 | 101.4119 | 4 | 5.3331 | -0.2766 | -1.7493 | 4.2657 | -0.2373 | 3.0936 | 0.1162 | 0.3795 | -0.1828 | -0.224 | -0.3935 | -0.4833 | 0.5429 | 0.686 | 0.2331 | 0.411 | 0.1632 | 0.0324 | 0.3799 | 0.0596 | 0.4632 | 12.9824 | 45.9854 | 92.5575 | 5 | 6.3549 | -2.0464 | 0.0966 | 2.2875 | -1.242 | 4.3237 | 0.1395 | 0.3249 | -0.2659 | -0.5226 | -0.0964 | 0.4959 | 0.4745 | -0.0553 | -0.0634 | 0.3891 | 0.0067 | -0.0658 | 0.2606 | 0.0036 | 0.4024 | -5.3969 | 42.2981 | 89.697 | 6 | 0.8633 | 0.5827 | -0.5341 | 1.4529 | -0.9443 | 1.5685 | -0.0612 | -0.1661 | -0.2122 | 0.1186 | -0.1337 | -0.4758 | -0.0153 | 0.2704 | 0.22 | 0.1694 | -0.0058 | -0.0488 | 0.2983 | 0.0062 | 0.3274 | 27.4605 | 75.8249 | 74.6345 | 7 | 7.3475 | -0.2304 | -3.019 | 3.4474 | 0.2029 | 5.6857 | 0.0257 | 0.2917 | 0.0678 | -0.2135 | 0.0637 | 0.1702 | -0.2575 | -0.3301 | -0.1005 | 0.1779 | -0.0147 | -0.0385 | 0.1835 | 0.0027 | 0.2047 | 11.1034 | 69.281 | 67.6948 | 8 | 3.8352 | 3.4489 | -0.0116 | 5.5134 | -0.7561 | 1.6136 | 0.0013 | -0.1019 | -0.2815 | 0.023 | -0.0511 | -0.4215 | -0.0124 | 0.2154 | 0.043 | 0.1518 | 0.0282 | -0.0311 | 0.248 | 0.0297 | 0.236 | 23.0477 | 71.9144 | 77.6797 | 9 | 0.9255 | 0.7352 | -1.1679 | 2.6726 | -2.0013 | 2.8541 | 0.2138 | -0.1111 | 0.0836 | 0.4667 | -0.0519 | 0.0564 | -0.4142 | 0.0505 | -0.1707 | 0.2962 | -0.0278 | -0.0165 | 0.2812 | -0.0527 | 0.2111 | 16.8624 | 87.9861 | 82.1255 | 10 | 2.6263 | 0.9085 | -1.1545 | 6.2628 | -2.366 | 3.2783 | -0.0972 | 0.6241 | 0.3995 | -0.1722 | 0.2008 | 0.3226 | -0.1802 | -0.1188 | -0.0753 | 0.3073 | -0.0994 | -0.061 | 0.4765 | 0.1041 | 0.4031 | 2.9907 | 115.47 | 18.7591 | 11 | 3.1016 | -3.0968 | -1.0302 | 7.269 | -2.9181 | 4.5401 | 0.302 | 0.5027 | 0.2523 | -0.5237 | -0.491 | -0.1171 | 0.6476 | -0.0091 | 0.1662 | 0.3832 | -0.1182 | 0.0122 | 0.589 | 0.0512 | 0.2506 | 15.18 | 106.3794 | 17.3012 | 12 | 3.7418 | 1.2903 | 2.8245 | 7.2994 | 5.6767 | 7.1344 | -0.0751 | 0.7649 | 0.3727 | -0.5271 | 0.276 | -0.3761 | -0.4868 | 0.7377 | -0.2824 | 0.3248 | -0.1073 | 0.044 | 0.5549 | 0.0912 | 0.364 | 20.6723 | 114.3156 | 18.9292 | 13 | 6.4388 | -3.3501 | 1.275 | 3.0581 | -2.1647 | 8.3339 | -0.0554 | 0.2172 | -0.0118 | 0.0763 | 0.1019 | -0.4925 | -0.2873 | 0.6101 | 0.0022 | 0.4263 | -0.2222 | 0.004 | 0.5887 | 0.1145 | 0.6619 | 22.7651 | 121.0017 | 24.4814 | 14 | 3.9528 | -2.3969 | -0.0328 | 3.1737 | -0.311 | 4.9246 | 0.0651 | -0.2023 | 0.5068 | 0.4203 | -0.0915 | 0.0128 | -0.5257 | -0.1998 | -0.0852 | 0.4514 | -0.0609 | -0.0295 | 0.357 | 0.1241 | 0.6004 | 3.362 | 125.146 | 28.061 | 15 | 0.8524 | 0.0927 | -0.4985 | 2.2746 | -1.2748 | 1.833 | -0.1734 | -0.0028 | -0.0484 | -0.0875 | 0.0236 | -0.2698 | 0.0745 | 0.0315 | 0.1497 | 0.1631 | -0.0454 | 0.0083 | 0.2507 | -0.0599 | 0.2718 | 29.5946 | 94.0576 | 48.2869 | 16 | 7.5485 | 2.2402 | 1.8188 | 4.4407 | -0.0356 | 4.6625 | -0.0239 | -0.0978 | -0.076 | -0.1829 | 0.0715 | 0.1267 | 0.1869 | -0.2827 | -0.042 | 0.1717 | 0.0428 | 0.0205 | 0.2184 | -0.0049 | 0.1799 | 11.9512 | 100.4579 | 51.8314 | 17 | 7.2057 | -1.8751 | -2.6006 | 3.5276 | 0.0434 | 5.3153 | 0.0235 | 0.1511 | 0.4469 | -0.0815 | 0.1469 | 0.0072 | -0.2631 | -0.2917 | -0.1725 | 0.2054 | -0.0248 | -0.0001 | 0.2302 | 0.0132 | 0.231 | 17.7828 | 106.1828 | 42.2133 | 18 | 5.921 | -5.4092 | 0.754 | 8.0436 | -1.6325 | 3.1536 | -0.4159 | -0.2505 | 0.0079 | -0.1295 | -0.0063 | -0.9905 | 0.2455 | 0.3811 | 0.3858 | 0.2232 | -0.0198 | 0.1136 | 0.2608 | 0.0053 | 0.4359 | 35.0813 | 88.5765 | 47.0257 | 19 | 1.0663 | -0.7377 | -1.1884 | 4.0549 | -0.9174 | 2.6257 | -0.5973 | 0.6005 | -0.1723 | -1.2793 | 0.3674 | -0.4231 | 0.6588 | -0.2573 | -0.0409 | 0.7041 | -0.139 | 0.1849 | 0.3388 | -0.068 | 0.2678 | 26.9065 | 81.8914 | 37.857 | 20 | 0.4168 | -1.2921 | 0.9314 | 4.656 | -2.4203 | 3.8636 | -0.025 | 0.199 | -0.2646 | -0.6913 | 0.2773 | 0.3995 | 0.5201 | -0.8471 | -0.2311 | 0.5277 | -0.2135 | -0.0003 | 0.4909 | -0.0642 | 0.3123 | 17.8569 | 91.2843 | 31.0031 | 21 | 0.5579 | 0.184 | 0.5164 | 2.6325 | -0.46 | 4.4194 | -0.2725 | 0.2163 | -0.3205 | -0.8056 | 0.165 | 0.0166 | 0.6547 | -0.443 | 0.1496 | 0.5987 | -0.1965 | 0.0744 | 0.357 | -0.0919 | 0.3252 | 20.1029 | 74.7284 | 45.6046 |
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精密化 TLSグループ | ID | Refine-ID | Refine TLS-ID | Selection details |
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1 | X-RAY DIFFRACTION | 1 | chain 'A' and (resid 1 through 42 )2 | X-RAY DIFFRACTION | 2 | chain 'A' and (resid 43 through 136 )3 | X-RAY DIFFRACTION | 3 | chain 'A' and (resid 137 through 159 )4 | X-RAY DIFFRACTION | 4 | chain 'A' and (resid 160 through 216 )5 | X-RAY DIFFRACTION | 5 | chain 'A' and (resid 217 through 269 )6 | X-RAY DIFFRACTION | 6 | chain 'B' and (resid 1 through 96 )7 | X-RAY DIFFRACTION | 7 | chain 'B' and (resid 97 through 160 )8 | X-RAY DIFFRACTION | 8 | chain 'B' and (resid 161 through 215 )9 | X-RAY DIFFRACTION | 9 | chain 'B' and (resid 216 through 383 )10 | X-RAY DIFFRACTION | 10 | chain 'C' and (resid 2 through 102 )11 | X-RAY DIFFRACTION | 11 | chain 'C' and (resid 103 through 136 )12 | X-RAY DIFFRACTION | 12 | chain 'C' and (resid 137 through 159 )13 | X-RAY DIFFRACTION | 13 | chain 'C' and (resid 160 through 177 )14 | X-RAY DIFFRACTION | 14 | chain 'C' and (resid 178 through 261 )15 | X-RAY DIFFRACTION | 15 | chain 'D' and (resid 1 through 95 )16 | X-RAY DIFFRACTION | 16 | chain 'D' | | | | | | | | | | | | | | | |
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