+Open data
-Basic information
Entry | Database: PDB / ID: 5cwt | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Crystal structure of Chaetomium thermophilum Nup57 | ||||||||||||||||||
Components | Nucleoporin NUP57 | ||||||||||||||||||
Keywords | TRANSPORT PROTEIN / nucleocytoplasmic transport | ||||||||||||||||||
Function / homology | Function and homology information | ||||||||||||||||||
Biological species | Chaetomium thermophilum (fungus) | ||||||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / AB INITIO PHASING / Resolution: 2.5 Å | ||||||||||||||||||
Authors | Bley, C.J. / Hoelz, A. | ||||||||||||||||||
Funding support | United States, United Kingdom, 5items
| ||||||||||||||||||
Citation | Journal: Science / Year: 2015 Title: Architecture of the fungal nuclear pore inner ring complex. Authors: Stuwe, T. / Bley, C.J. / Thierbach, K. / Petrovic, S. / Schilbach, S. / Mayo, D.J. / Perriches, T. / Rundlet, E.J. / Jeon, Y.E. / Collins, L.N. / Huber, F.M. / Lin, D.H. / Paduch, M. / ...Authors: Stuwe, T. / Bley, C.J. / Thierbach, K. / Petrovic, S. / Schilbach, S. / Mayo, D.J. / Perriches, T. / Rundlet, E.J. / Jeon, Y.E. / Collins, L.N. / Huber, F.M. / Lin, D.H. / Paduch, M. / Koide, A. / Lu, V. / Fischer, J. / Hurt, E. / Koide, S. / Kossiakoff, A.A. / Hoelz, A. | ||||||||||||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 5cwt.cif.gz | 72.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb5cwt.ent.gz | 55.1 KB | Display | PDB format |
PDBx/mmJSON format | 5cwt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5cwt_validation.pdf.gz | 445.5 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 5cwt_full_validation.pdf.gz | 447.5 KB | Display | |
Data in XML | 5cwt_validation.xml.gz | 7.3 KB | Display | |
Data in CIF | 5cwt_validation.cif.gz | 9.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cw/5cwt ftp://data.pdbj.org/pub/pdb/validation_reports/cw/5cwt | HTTPS FTP |
-Related structure data
Related structure data | 4jnuC 4jnvC 4jo7C 4jo9C 4jq5C 5cwsC 5cwuC 5cwvC 5cwwC C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
| ||||||||
Details | Nup57 is a homo-dimer in solution by SEC-MALS |
-Components
#1: Protein | Mass: 6299.908 Da / Num. of mol.: 4 / Fragment: UNP residues 265-317 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: NUP57, CTHT_0010940 / Production host: Escherichia coli (E. coli) / References: UniProt: G0S0R2 #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 50.06 % |
---|---|
Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 5 % (w/v) PEG 1,000 12 % (w/v) PEG 8,000 |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 17, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→20 Å / Num. all: 8970 / Num. obs: 8970 / % possible obs: 98 % / Redundancy: 15.2 % / Rsym value: 0.043 / Net I/σ(I): 58.3 |
-Processing
Software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 2.5→19.256 Å / Cross valid method: FREE R-VALUE / Stereochemistry target values: ML
| ||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→19.256 Å
| ||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||
LS refinement shell | Resolution: 2.5→2.6549 Å /
|