PDB-5cwu: Crystal structure of Chaetomium thermophilum Nup188 TAIL domain

ID or keywords:

Entry

Database: PDB / ID: 5cwu

Title

Crystal structure of Chaetomium thermophilum Nup188 TAIL domain

Components

Nucleoporin NUP188

Keywords

TRANSPORT PROTEIN / nucleocytoplasmic transport

Function / homology

Function and homology information

nuclear pore inner ring / structural constituent of nuclear pore / RNA export from nucleus / mRNA transport / protein import into nucleus / nuclear membrane
Similarity search - Function

Biological species

Chaetomium thermophilum (fungus)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

SAD / Resolution: 3.35 Å

Authors

Stuwe, T. / Bley, C.J. / Thierbach, K. / Petrovic, S. / Schilbach, S. / Mayo, D.J. / Perriches, T. / Rundlet, E.J. / Jeon, Y.E. / Collins, L.N. ...

Funding support

United States, 5items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R01-GM111461	United States
V Foundation for Cancer Research	Albert Wyrick V Scholar Award	United States
Edward Mallinckrodt Jr. Foundation	54th Mallinckrodt Scholar Award	United States
Sidney Kimmel Foundation for Cancer Research	Kimmel Scholar Award	United States
The Camille & Henry Dreyfus Foundation	Camille-Dreyfus Teacher Scholar Award	United States

Citation

Journal: Science / Year: 2015
Title: Architecture of the fungal nuclear pore inner ring complex.
Authors: Stuwe, T. / Bley, C.J. / Thierbach, K. / Petrovic, S. / Schilbach, S. / Mayo, D.J. / Perriches, T. / Rundlet, E.J. / Jeon, Y.E. / Collins, L.N. / Huber, F.M. / Lin, D.H. / Paduch, M. / ...

History

Deposition	Jul 28, 2015	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Sep 16, 2015	Provider: repository / Type: Initial release
Revision 1.1	Oct 14, 2015	Group: Database references
Revision 1.2	Jul 20, 2016	Group: Data collection
Revision 1.3	Sep 27, 2017	Group: Author supporting evidence / Derived calculations / Refinement description Category: pdbx_audit_support / pdbx_struct_oper_list / software Item: _pdbx_audit_support.funding_organization / _pdbx_struct_oper_list.symmetry_operation / _software.classification
Revision 1.4	Nov 1, 2017	Group: Author supporting evidence / Category: pdbx_struct_assembly_auth_evidence
Revision 1.5	Dec 25, 2019	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	5cwu.cif.gz	936.3 KB	Display	PDBx/mmCIF format
PDB format	pdb5cwu.ent.gz	811.8 KB	Display	PDB format
PDBx/mmJSON format	5cwu.json.gz		Tree view	PDBx/mmJSON format
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Validation report

Summary document	5cwu_validation.pdf.gz	523.5 KB	Display	wwPDB validaton report
Full document	5cwu_full_validation.pdf.gz	549.8 KB	Display
Data in XML	5cwu_validation.xml.gz	78.2 KB	Display
Data in CIF	5cwu_validation.cif.gz	100.8 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/cw/5cwu ftp://data.pdbj.org/pub/pdb/validation_reports/cw/5cwu	HTTPS FTP

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Related structure data

Related structure data	4jnuC 4jnvC 4jo7C 4jo9C 4jq5C 5cwsC 5cwtC 5cwvC 5cwwC C: citing same article (ref.)
Similar structure data	3e2k-1 3e2l-1 7bov-d 6cwf-d 6cwl-d 1v6c-2 5eg9-d 1msk-d 1w9h-d 5u7p-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

Related structure data

C: citing same article (ref.)

Similar structure data

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: Nucleoporin NUP188

B: Nucleoporin NUP188

C: Nucleoporin NUP188

D: Nucleoporin NUP188

E: Nucleoporin NUP188

F: Nucleoporin NUP188

G: Nucleoporin NUP188

H: Nucleoporin NUP188

hetero molecules

356 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Nucleoporin NUP188

hetero molecules

defined by author&software
Evidence: gel filtration, light scattering
44.6 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Nucleoporin NUP188

hetero molecules

defined by author&software
Evidence: gel filtration, light scattering
44.6 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

C: Nucleoporin NUP188

hetero molecules

defined by author&software
Evidence: gel filtration, light scattering
44.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

D: Nucleoporin NUP188

hetero molecules

defined by author&software
Evidence: gel filtration, light scattering
44.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

5

E: Nucleoporin NUP188

hetero molecules

defined by author&software
Evidence: gel filtration, light scattering
44.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

6

F: Nucleoporin NUP188

defined by author&software
Evidence: gel filtration, light scattering
44.4 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

7

G: Nucleoporin NUP188

hetero molecules

defined by author&software
Evidence: gel filtration, light scattering
44.5 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

8

H: Nucleoporin NUP188

defined by author&software
Evidence: gel filtration, light scattering
44.4 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	65.276, 65.278, 155.548
Angle α, β, γ (deg.)	90.00, 89.95, 90.01
Int Tables number	1
Space group name H-M	P1

Details

Monomer by SEC-MALS

#1: Protein	Nucleoporin NUP188 / Nuclear pore protein NUP188 Mass: 44428.438 Da / Num. of mol.: 8 / Fragment: UNP residues 1447-1858 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: NUP188, CTHT_0070850 / Production host: Escherichia coli (E. coli) / References: UniProt: G0SFH5
#2: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C₃H₈O₃

#1: Protein

Nucleoporin NUP188 / Nuclear pore protein NUP188

Mass: 44428.438 Da / Num. of mol.: 8 / Fragment: UNP residues 1447-1858
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: NUP188, CTHT_0070850 / Production host:

Escherichia coli (E. coli) / References: UniProt: G0SFH5

#2: Chemical

ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL

Mass: 92.094 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C₃H₈O₃

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Experiment

Experiment	Method: X-RAY DIFFRACTION

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Sample preparation

Crystal	Density Matthews: 1.87 Å³/Da / Density % sol: 34.32 %
Crystal grow	Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 6.9 Details: 100 mM HEPES, pH 6.9 16 % (w/v) PEG 3,350 200 mM ammonium citrate PH range: 6.9

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 0.97944 Å
Detector	Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Nov 2, 2013
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.97944 Å / Relative weight: 1
Reflection	Resolution: 3.35→20 Å / Num. obs: 33102 / % possible obs: 99 % / Redundancy: 4 % / R_sym value: 0.054 / Net I/σ(I): 23.4

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Processing

Software

Name	Version	Classification
PHENIX	1.8.2_1309	refinement
HKL-2000		data scaling
SCALEPACK		data scaling
PHENIX		phasing

Refinement

Method to determine structure:

SAD / Resolution: 3.35→19.853 Å / SU ML: 0.55 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 31.02 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.2792	3632	4.99 %	Random selection
R_work	0.2462	-	-	-
obs	0.2477	33102	98.82 %	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 3.35→19.853 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	18256	0	48	0	18304

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.004	18584
X-RAY DIFFRACTION	f_angle_d	0.734	25288
X-RAY DIFFRACTION	f_dihedral_angle_d	11.999	6616
X-RAY DIFFRACTION	f_chiral_restr	0.029	3152
X-RAY DIFFRACTION	f_plane_restr	0.004	3152

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
3.35-3.3939	0.3858	152	0.3869	2796	X-RAY DIFFRACTION	97
3.3939-3.4401	0.3201	140	0.3773	2718	X-RAY DIFFRACTION	98
3.4401-3.489	0.3206	136	0.3355	2496	X-RAY DIFFRACTION	98
3.489-3.5408	0.3185	124	0.3551	2593	X-RAY DIFFRACTION	98
3.5408-3.5958	0.4015	148	0.3397	2570	X-RAY DIFFRACTION	98
3.5958-3.6544	0.3425	136	0.365	2774	X-RAY DIFFRACTION	99
3.6544-3.717	0.3135	128	0.3497	2512	X-RAY DIFFRACTION	99
3.717-3.7842	0.4307	134	0.3332	2732	X-RAY DIFFRACTION	99
3.7842-3.8564	0.3916	150	0.332	2706	X-RAY DIFFRACTION	99
3.8564-3.9346	0.4929	140	0.2819	2603	X-RAY DIFFRACTION	99
3.9346-4.0194	0.3508	168	0.3449	2714	X-RAY DIFFRACTION	99
4.0194-4.1121	0.4044	120	0.3073	2637	X-RAY DIFFRACTION	99
4.1121-4.214	0.4666	144	0.2966	2660	X-RAY DIFFRACTION	99
4.214-4.3268	0.2373	156	0.2649	2652	X-RAY DIFFRACTION	99
4.3268-4.4527	0.3235	136	0.2517	2616	X-RAY DIFFRACTION	99
4.4527-4.5947	0.3146	138	0.2675	2738	X-RAY DIFFRACTION	99
4.5947-4.7568	0.249	136	0.2774	2574	X-RAY DIFFRACTION	99
4.7568-4.9444	0.3089	160	0.2763	2755	X-RAY DIFFRACTION	99
4.9444-5.1657	0.3333	144	0.2725	2618	X-RAY DIFFRACTION	99
5.1657-5.4327	0.2716	160	0.212	2747	X-RAY DIFFRACTION	100
5.4327-5.7653	0.1921	140	0.2448	2610	X-RAY DIFFRACTION	100
5.7653-6.1979	0.3138	126	0.2697	2642	X-RAY DIFFRACTION	99
6.1979-6.7987	0.3228	136	0.2925	2658	X-RAY DIFFRACTION	100
6.7987-7.7312	0.264	136	0.2498	2732	X-RAY DIFFRACTION	100
7.7312-9.5562	0.2196	122	0.1653	2685	X-RAY DIFFRACTION	100
9.5562-19.8536	0.1382	122	0.1441	2582	X-RAY DIFFRACTION	96

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.1334	3.5071	1.5224	6.5662	0.9024	4.3366	0.5355	0.7325	-1.186	0.5516	0.7208	-3.1904	1.3506	1.4873	-1.1051	1.2048	0.3828	-0.3984	0.9851	-0.3571	1.708	18.4314	-9.3005	5.2783
2	2.7939	-1.7341	-0.0855	3.8596	-0.2066	0.7724	0.2113	-0.1176	-0.4436	0.8359	0.1145	-0.4471	0.0511	0.0849	-0.0119	0.8159	0.2114	-0.4116	0.2886	-0.0769	0.7687	12.0428	-7.078	8.7978
3	4.4945	-0.2556	3.3901	4.9945	0.1435	6.2582	0.1623	1.7235	-0.1906	-0.467	0.0839	-0.4927	-0.4827	1.8283	0.2006	0.5591	-0.0232	-0.1251	0.7883	-0.073	0.2085	17.8124	7.3171	9.2044
4	3.0509	0.8209	3.3763	2.4741	0.7237	7.6256	0.2395	-0.1719	-0.3171	1.139	0.1193	-0.2624	0.1012	-0.315	0.0757	0.9477	0.0541	-0.4231	0.3472	-0.0724	0.423	10.546	3.7762	17.5028
5	4.9012	2.1135	0.2959	4.603	2.1137	7.1308	-0.1119	-0.8935	1.0115	0.9415	0.0089	0.6665	-1.3271	-0.8483	0.449	1.0125	0.1099	-0.0816	0.7421	-0.2958	0.497	1.7695	14.4336	18.5868
6	2.3748	0.998	2.8637	1.4493	-0.8243	7.4289	0.0479	-1.2848	0.7647	0.5423	0.703	-0.3856	0.189	-0.0373	-0.5498	2.0567	0.5446	0.2253	1.6739	-0.5261	1.2454	-9.2126	16.9179	25.5868
7	3.6527	1.0418	0.2042	5.535	-6.6681	8.6484	0.7707	-1.3432	1.6228	0.3971	1.0819	1.5172	0.9514	-1.9363	-1.7387	1.2382	-0.3522	0.2539	2.5253	-0.0614	1.3439	-22.6639	9.8911	14.2863
8	1.9652	-3.1161	-1.4962	6.0166	1.2274	2.3782	0.2183	-0.4704	0.7622	-0.5084	0.9703	-2.4491	-0.3325	0.9452	-1.0871	1.0159	-0.2423	0.2003	0.6733	-0.3687	1.4109	51.0195	42.4292	-44.445
9	4.0552	1.3783	-3.4473	4.1984	-0.9851	2.9311	0.1592	-0.522	0.568	-0.7966	0.2049	-0.9344	-0.3272	0.6128	-0.0987	0.6633	-0.1524	0.2227	0.3312	-0.2116	0.4801	46.6256	35.0827	-48.141
10	0.5746	-0.4252	-0.4667	0.6122	0.9288	1.6591	0.1368	0.0814	0.1069	-0.6141	-0.1096	-0.0575	0.1318	-0.0856	0.3847	1.1578	-0.0564	0.4842	0.3311	-0.2618	0.5927	43.0434	29.3316	-56.7159
11	4.1946	-2.0296	-1.1378	1.3674	1.1992	7.8044	-0.2088	0.831	-1.1123	-0.8654	0.235	0.3715	1.1154	-0.6505	0.3318	1.0401	-0.0011	-0.0246	0.6363	-0.3365	0.5527	34.3404	18.6568	-57.7216
12	4.3458	0.585	-2.2358	2.5668	-1.3676	2.283	-0.6043	1.2795	-0.058	-1.4874	0.8289	0.1446	0.581	-0.53	-0.01	1.8768	-0.291	-0.2907	1.8539	-0.5977	1.161	23.3144	16.1748	-64.7543
13	0.5917	0.2399	0.2715	6.1565	-7.4661	9.5921	0.9283	1.7349	-2.5122	0.1454	0.92	0.8056	-0.7615	-1.1947	-1.7748	1.2506	0.4	-0.4495	2.9692	-0.1062	1.7372	9.9019	23.2162	-53.4325
14	4.8023	-3.1196	1.1483	2.6011	0.2286	6.3713	0.5907	-0.3993	-2.2258	-0.6805	0.9552	0.7503	1.7684	-1.0907	-1.3185	0.9722	-0.2548	-0.2828	1.0199	0.4232	1.5573	23.4094	14.6965	44.1347
15	2.5866	-0.4601	1.2076	4.7666	-3.823	3.3355	0.3736	-0.5275	-0.7446	-0.8828	0.2583	1.0581	0.7504	-0.1906	-0.4492	0.4057	-0.1449	-0.2026	0.6119	0.1778	0.5205	30.7372	19.1113	47.843
16	2.7198	-1.0549	1.8313	2.2512	-2.3424	4.852	0.0121	-0.9723	-0.2357	0.1857	0.3274	0.317	-0.0145	-0.1874	0.1137	0.3131	-0.0206	-0.1574	0.9625	0.4607	0.5074	36.4866	22.6305	56.3829
17	3.3176	-1.075	-0.1149	3.6803	-1.3171	9.2533	0.2121	-1.1772	0.5175	0.9325	-0.0956	-0.9176	-1.003	1.1607	0.1918	0.7649	-0.018	-0.3491	0.9078	0.0011	0.5738	47.1462	31.3879	57.452
18	2.7561	-0.7578	-0.6703	3.6671	-4.5704	6.6966	0.8683	-0.366	-0.846	1.6133	0.0252	-0.8598	-0.5507	0.167	-0.7387	1.8047	-0.4324	-0.5308	2.0407	-0.266	1.1971	49.621	42.3904	64.4651
19	8.0457	0.0574	-7.7912	2.8987	-0.5772	7.6363	1.475	0.1388	0.8118	0.9691	0.8095	-1.9534	-1.2539	-0.3795	-2.1069	2.7081	0.5188	-0.1813	1.2385	-0.3738	1.2767	42.5512	55.8278	53.1714
20	7.1458	-3.7992	-0.7601	2.699	2.2421	5.0917	0.7622	-0.572	3.0155	-0.9128	0.5168	-1.1097	-1.5552	1.03	-1.1029	0.9147	-0.2608	0.3673	0.9756	-0.3329	1.8055	42.4318	51.0327	-33.64
21	3.3779	1.003	1.0017	2.2781	-0.0475	1.5385	0.2084	-0.7351	0.2438	0.1079	0.2154	-0.3033	0.0599	-0.0196	0.0173	0.236	-0.1799	0.0207	0.7224	-0.4103	0.712	40.1795	44.6056	-30.0672
22	6.876	1.1985	-3.0871	5.3631	3.1743	6.6126	0.1635	0.9511	0.783	-1.9009	0.2349	-0.0785	-1.64	-0.3803	-0.0937	0.711	0.0999	0.0372	0.4074	-0.0555	0.2512	25.7934	50.3727	-29.6808
23	2.5896	-0.3789	0.0123	2.7641	3.1928	5.4399	0.0717	-1.1315	0.2657	0.1208	0.2729	-0.3583	0.2562	0.04	0.1835	0.345	-0.0324	0.0599	0.9733	-0.4343	0.4385	29.3274	43.0848	-21.3866
24	3.9939	-1.5901	-0.0006	5.934	1.5465	8.7176	0.178	-1.2508	-0.5918	1.0307	-0.17	1.1503	0.9194	-1.2214	0.2193	0.735	-0.0312	0.3088	0.9114	-0.067	0.5346	18.7086	34.329	-20.3106
25	3.1064	-0.1175	1.6968	4.6048	2.0591	1.9204	0.5411	-1.0386	-0.1753	1.672	-0.1998	0.6398	-0.1162	0.2611	-0.1468	1.7471	-0.3059	0.4841	1.8498	0.3104	1.1624	16.2087	23.3364	-13.2806
26	5.9804	-0.4094	6.5963	3.6106	-0.0276	7.3694	1.3443	-0.0131	-1.4376	1.034	0.8062	1.8877	1.3274	0.5959	-2.0633	2.732	0.5243	0.0182	1.3108	0.3489	1.4731	23.2694	9.8889	-24.5865
27	5.4395	0.3903	3.2445	0.804	-0.3415	5.8334	1.1824	0.5139	-2.2725	0.2484	0.5671	-0.2458	1.2887	0.2623	-1.5698	0.8112	0.2959	-0.4806	0.9129	-0.3256	1.5429	75.1128	-17.8996	-5.5624
28	2.5131	-1.6416	-1.3324	3.2059	2.9515	2.7263	0.0432	0.7985	-0.8335	0.4545	0.2834	-0.5534	0.4946	0.1976	0.0251	0.3458	0.1787	-0.2256	0.7253	-0.2997	0.5667	67.7681	-13.5354	-9.2379
29	0.489	0.6016	0.9508	1.3004	1.3864	3.0204	-0.0866	0.6437	-0.1652	-0.1153	0.2835	-0.2577	-0.1251	0.0308	0.425	0.4299	0.2012	-0.2549	1.0879	-0.5282	0.5588	61.9761	-9.9541	-17.8082
30	2.3537	2.659	0.6432	3.8727	0.533	6.7092	0.0618	0.9566	0.4295	-0.7873	-0.2051	0.9986	-0.6503	-1.2446	0.6351	0.7771	0.1591	-0.3666	1.0394	-0.1199	0.5252	51.3385	-1.2426	-18.8272
31	1.9063	1.3791	0.1836	2.7532	3.7683	7.5895	0.4529	0.9662	-0.1107	-1.4566	-0.0024	0.5541	-0.2702	0.0636	-0.2528	1.7618	0.3837	-0.6131	1.9517	0.3092	1.1688	48.8425	9.7527	-25.8789
32	7.6893	-0.1498	-6.9135	2.2924	-2.1053	8.4048	0.9435	0.26	1.2775	-1.4102	0.6206	2.0974	-1.4127	0.6182	-1.4201	2.7311	-0.374	-0.1089	1.1424	0.4565	1.6429	55.9003	23.2051	-14.5792
33	4.0243	2.8593	-1.1473	3.6353	-1.1011	7.441	0.4805	0.6384	0.7082	0.2645	0.9178	2.0165	-1.4665	-1.7169	-1.2727	1.1162	0.2578	0.2323	0.8692	0.2902	1.2879	-17.9204	9.7579	-72.4845
34	1.9992	-1.0757	-0.0769	4.742	1.3354	0.792	0.1309	-0.1518	0.4596	1.018	0.1212	0.4266	0.0616	-0.113	-0.0176	0.8807	0.1958	0.3437	0.2839	0.1179	0.7394	-11.5017	7.5302	-68.9512
35	6.2703	0.601	-6.2445	4.0994	-2.7008	7.368	0.0562	2.1693	0.3077	-0.9973	0.2687	1.0158	0.3341	-1.5841	-0.2231	0.4756	-0.02	-0.0066	0.6894	0.1215	0.3494	-17.2809	-6.8336	-68.5791
36	3.3064	0.8886	-3.0401	6.7156	-2.0055	7.8749	0.4725	-0.1756	0.446	1.2972	0.2753	-0.054	-0.0666	-0.0007	-0.3149	0.7912	0.0675	0.2999	0.2783	0.0889	0.4765	-9.9804	-3.3101	-60.2955
37	5.1611	2.0255	0.225	4.1843	-2.1411	6.7918	-0.0297	-0.9743	-1.1337	0.9958	-0.0094	-0.6252	1.1513	0.6908	0.3781	0.9381	0.1269	0.0426	0.675	0.3642	0.5194	-1.2437	-13.9687	-59.1986
38	1.9453	0.7659	-1.996	1.4706	1.143	5.2195	-0.5226	-1.3222	-0.5007	1.391	0.6845	-0.1775	-0.3503	0.5038	0.0449	1.9867	0.4449	-0.2964	1.7765	0.4659	1.285	9.7665	-16.4566	-52.1962
39	0.0207	0.0993	0.1224	7.3288	7.2669	7.2293	0.6639	-1.3356	-2.259	-0.1307	1.3944	-0.8842	-0.2967	0.8172	-1.8983	1.0765	-0.3084	-0.4168	2.7576	0.0517	1.5694	23.2093	-9.3724	-63.4385
40	3.2982	1.2766	-3.266	1.7991	-1.9726	4.5021	1.123	0.0785	2.1458	0.76	0.4633	0.3551	-1.5799	-0.9783	-1.5363	0.904	0.3348	0.3488	1.0406	0.1267	1.4079	-9.2219	18.3629	72.1947
41	5.5302	-0.1264	0.76	2.9955	-2.7525	2.5968	-0.0083	0.6535	0.7615	0.7674	0.5407	0.8293	-0.6265	-0.3067	-0.1539	0.4124	0.2186	0.1004	0.584	0.2431	0.5158	-1.8906	14.0066	68.5448
42	1.8418	0.838	-2.8605	2.5287	-2.3331	6.5654	-0.0416	0.8782	0.046	-0.1307	0.3329	0.3536	0.1768	-0.3913	-0.0672	0.2962	0.0926	0.0918	1.0585	0.417	0.467	3.8607	10.4311	59.9456
43	4.0469	1.6385	-1.2488	6.3521	-1.3465	5.2975	0.1246	1.1112	-0.5838	-0.8651	-0.2869	-1.0073	0.9536	1.1767	0.3398	0.7726	0.1328	0.229	0.9565	0.0191	0.414	14.5051	1.6876	58.9265
44	1.9168	1.2058	0.6726	2.679	-3.3377	7.5937	0.521	1.1587	0.1804	-1.4939	0.0391	-0.7775	0.2967	-0.6233	-0.2815	1.8856	0.4744	0.5936	1.8779	-0.2375	1.192	16.9984	-9.2991	51.8964
45	7.1135	-0.8385	6.0688	2.846	2.1431	8.1484	0.626	0.1041	-1.5203	-1.3935	0.427	-2.2387	1.3435	-1.0605	-0.9907	2.6648	-0.4423	0.119	1.0926	-0.3946	1.7915	9.983	-22.755	63.213
46	1.1887	-1.9004	0.5709	8.4962	-3.4642	7.3338	0.7238	-0.4611	-0.7698	-0.7689	1.3002	2.1326	0.4013	-1.5005	-1.9024	0.9659	-0.1977	-0.2809	0.7993	0.4051	1.4085	14.7575	23.2842	33.3717
47	5.1784	1.2649	4.4163	3.955	1.6018	4.6549	0.4383	-0.7194	-0.9823	-0.7258	0.0771	0.6548	0.1681	-0.4812	-0.3318	0.6443	-0.1316	-0.2457	0.3375	0.1453	0.4867	19.165	30.6521	29.6497
48	4.4776	-2.7221	1.7662	4.0642	0.0369	6.2492	0.0497	0.5973	0.4319	-1.2063	0.0892	-0.306	-0.4985	0.6897	0.7946	0.9692	-0.0294	-0.2859	0.5048	0.2143	0.2907	28.0168	42.8652	20.4652
49	5.0984	-0.1996	5.8203	4.1316	0.7923	6.8997	-0.472	2.2061	0.6071	-0.9408	1.1104	0.3718	-1.1466	0.7939	-0.4466	1.7842	-0.3279	0.2504	1.7654	0.4956	1.1324	42.4629	49.568	13.0403
50	1.889	-0.0097	0.5252	5.5403	6.0391	7.5432	0.3736	1.4853	1.9389	-0.1301	0.9881	-1.5301	1.263	1.8873	-1.3218	1.329	0.3659	0.4414	2.5623	0.0185	1.6905	55.9176	42.4993	24.2966

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 1450:1473 )	A	1450 - 1473
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 1474:1520 )	A	1474 - 1520
3	X-RAY DIFFRACTION	3	( CHAIN A AND RESID 1521:1543 )	A	1521 - 1543
4	X-RAY DIFFRACTION	4	( CHAIN A AND RESID 1544:1592 )	A	1544 - 1592
5	X-RAY DIFFRACTION	5	( CHAIN A AND RESID 1615:1674 )	A	1615 - 1674
6	X-RAY DIFFRACTION	6	( CHAIN A AND RESID 1675:1760 )	A	1675 - 1760
7	X-RAY DIFFRACTION	7	( CHAIN A AND RESID 1761:1799 )	A	1761 - 1799
8	X-RAY DIFFRACTION	8	( CHAIN B AND RESID 1450:1473 )	B	1450 - 1473
9	X-RAY DIFFRACTION	9	( CHAIN B AND RESID 1474:1543 )	B	1474 - 1543
10	X-RAY DIFFRACTION	10	( CHAIN B AND RESID 1544:1592 )	B	1544 - 1592
11	X-RAY DIFFRACTION	11	( CHAIN B AND RESID 1615:1674 )	B	1615 - 1674
12	X-RAY DIFFRACTION	12	( CHAIN B AND RESID 1675:1760 )	B	1675 - 1760
13	X-RAY DIFFRACTION	13	( CHAIN B AND RESID 1761:1799 )	B	1761 - 1799
14	X-RAY DIFFRACTION	14	( CHAIN C AND RESID 1450:1473 )	C	1450 - 1473
15	X-RAY DIFFRACTION	15	( CHAIN C AND RESID 1474:1543 )	C	1474 - 1543
16	X-RAY DIFFRACTION	16	( CHAIN C AND RESID 1544:1592 )	C	1544 - 1592
17	X-RAY DIFFRACTION	17	( CHAIN C AND RESID 1615:1674 )	C	1615 - 1674
18	X-RAY DIFFRACTION	18	( CHAIN C AND RESID 1675:1760 )	C	1675 - 1760
19	X-RAY DIFFRACTION	19	( CHAIN C AND RESID 1761:1799 )	C	1761 - 1799
20	X-RAY DIFFRACTION	20	( CHAIN D AND RESID 1450:1473 )	D	1450 - 1473
21	X-RAY DIFFRACTION	21	( CHAIN D AND RESID 1474:1520 )	D	1474 - 1520
22	X-RAY DIFFRACTION	22	( CHAIN D AND RESID 1521:1543 )	D	1521 - 1543
23	X-RAY DIFFRACTION	23	( CHAIN D AND RESID 1544:1592 )	D	1544 - 1592
24	X-RAY DIFFRACTION	24	( CHAIN D AND RESID 1615:1674 )	D	1615 - 1674
25	X-RAY DIFFRACTION	25	( CHAIN D AND RESID 1675:1760 )	D	1675 - 1760
26	X-RAY DIFFRACTION	26	( CHAIN D AND RESID 1761:1799 )	D	1761 - 1799
27	X-RAY DIFFRACTION	27	( CHAIN E AND RESID 1450:1473 )	E	1450 - 1473
28	X-RAY DIFFRACTION	28	( CHAIN E AND RESID 1474:1543 )	E	1474 - 1543
29	X-RAY DIFFRACTION	29	( CHAIN E AND RESID 1544:1592 )	E	1544 - 1592
30	X-RAY DIFFRACTION	30	( CHAIN E AND RESID 1615:1674 )	E	1615 - 1674
31	X-RAY DIFFRACTION	31	( CHAIN E AND RESID 1675:1760 )	E	1675 - 1760
32	X-RAY DIFFRACTION	32	( CHAIN E AND RESID 1761:1799 )	E	1761 - 1799
33	X-RAY DIFFRACTION	33	( CHAIN F AND RESID 1450:1473 )	F	1450 - 1473
34	X-RAY DIFFRACTION	34	( CHAIN F AND RESID 1474:1520 )	F	1474 - 1520
35	X-RAY DIFFRACTION	35	( CHAIN F AND RESID 1521:1543 )	F	1521 - 1543
36	X-RAY DIFFRACTION	36	( CHAIN F AND RESID 1544:1592 )	F	1544 - 1592
37	X-RAY DIFFRACTION	37	( CHAIN F AND RESID 1615:1674 )	F	1615 - 1674
38	X-RAY DIFFRACTION	38	( CHAIN F AND RESID 1675:1760 )	F	1675 - 1760
39	X-RAY DIFFRACTION	39	( CHAIN F AND RESID 1761:1799 )	F	1761 - 1799
40	X-RAY DIFFRACTION	40	( CHAIN G AND RESID 1450:1473 )	G	1450 - 1473
41	X-RAY DIFFRACTION	41	( CHAIN G AND RESID 1474:1543 )	G	1474 - 1543
42	X-RAY DIFFRACTION	42	( CHAIN G AND RESID 1544:1592 )	G	1544 - 1592
43	X-RAY DIFFRACTION	43	( CHAIN G AND RESID 1615:1674 )	G	1615 - 1674
44	X-RAY DIFFRACTION	44	( CHAIN G AND RESID 1675:1760 )	G	1675 - 1760
45	X-RAY DIFFRACTION	45	( CHAIN G AND RESID 1761:1799 )	G	1761 - 1799
46	X-RAY DIFFRACTION	46	( CHAIN H AND RESID 1450:1473 )	H	1450 - 1473
47	X-RAY DIFFRACTION	47	( CHAIN H AND RESID 1474:1543 )	H	1474 - 1543
48	X-RAY DIFFRACTION	48	( CHAIN H AND RESID 1544:1674 )	H	1544 - 1674
49	X-RAY DIFFRACTION	49	( CHAIN H AND RESID 1675:1760 )	H	1675 - 1760
50	X-RAY DIFFRACTION	50	( CHAIN H AND RESID 1761:1799 )	H	1761 - 1799

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