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- PDB-5cws: Crystal structure of the intact Chaetomium thermophilum Nsp1-Nup4... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5cws | ||||||||||||||||||
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Title | Crystal structure of the intact Chaetomium thermophilum Nsp1-Nup49-Nup57 channel nucleoporin heterotrimer bound to its Nic96 nuclear pore complex attachment site | ||||||||||||||||||
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![]() | PROTEIN TRANSPORT / nucleocytoplasmic transport | ||||||||||||||||||
Function / homology | ![]() structural constituent of nuclear pore / poly(A)+ mRNA export from nucleus / nuclear localization sequence binding / mRNA transport / nuclear pore / protein import into nucleus / protein transport / nuclear membrane Similarity search - Function | ||||||||||||||||||
Biological species | ![]() ![]() | ||||||||||||||||||
Method | ![]() ![]() | ||||||||||||||||||
![]() | Bley, C.J. / Petrovic, S. / Paduch, M. / Lu, V. / Kossiakoff, A.A. / Hoelz, A. | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Architecture of the fungal nuclear pore inner ring complex. Authors: Stuwe, T. / Bley, C.J. / Thierbach, K. / Petrovic, S. / Schilbach, S. / Mayo, D.J. / Perriches, T. / Rundlet, E.J. / Jeon, Y.E. / Collins, L.N. / Huber, F.M. / Lin, D.H. / Paduch, M. / ...Authors: Stuwe, T. / Bley, C.J. / Thierbach, K. / Petrovic, S. / Schilbach, S. / Mayo, D.J. / Perriches, T. / Rundlet, E.J. / Jeon, Y.E. / Collins, L.N. / Huber, F.M. / Lin, D.H. / Paduch, M. / Koide, A. / Lu, V. / Fischer, J. / Hurt, E. / Koide, S. / Kossiakoff, A.A. / Hoelz, A. | ||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 781.6 KB | Display | ![]() |
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PDB format | ![]() | 667.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 518.1 KB | Display | ![]() |
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Full document | ![]() | 530.3 KB | Display | |
Data in XML | ![]() | 68.7 KB | Display | |
Data in CIF | ![]() | 93.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4jnuC ![]() 4jnvC ![]() 4jo7C ![]() 4jo9C ![]() 4jq5C ![]() 5cwtC ![]() 5cwuC ![]() 5cwvC ![]() 5cwwC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 4 types, 8 molecules CIDJEKFL
#3: Protein | Mass: 23558.211 Da / Num. of mol.: 2 / Fragment: UNP residues 467-674 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: NSP1, CTHT_0054390 / Production host: ![]() ![]() #4: Protein | Mass: 24536.504 Da / Num. of mol.: 2 / Fragment: UNP residues 246-470 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #5: Protein | Mass: 28792.684 Da / Num. of mol.: 2 / Fragment: UNP residues 74-319 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: NUP57, CTHT_0010940 / Production host: ![]() ![]() #6: Protein | Mass: 7793.831 Da / Num. of mol.: 2 / Fragment: UNP residues 139-211 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: NIC96, CTHT_0008480 / Production host: ![]() ![]() |
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-Antibody , 2 types, 4 molecules AGBH
#1: Antibody | Mass: 25394.451 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Antibody | Mass: 28606.086 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 1 types, 2 molecules ![](data/chem/img/OS.gif)
#7: Chemical |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.85 Å3/Da / Density % sol: 68.07 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 8.7 Details: 0.1M TRIS, pH 8.7 5.7 % (w/v) PEG 20,000 1 % (v/v) 1-propanol PH range: 8.7 - 8.9 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 25, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.14 Å / Relative weight: 1 |
Reflection | Resolution: 3.77→50 Å / Num. obs: 43896 / % possible obs: 99.9 % / Redundancy: 40.2 % / Rmerge(I) obs: 0.204 / Net I/σ(I): 17 |
Reflection shell | Highest resolution: 3.77 Å |
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Processing
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Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 3.77→49.666 Å / SU ML: 0.64 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 30.79 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.77→49.666 Å
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Refine LS restraints |
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LS refinement shell |
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