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Yorodumi- PDB-6t8c: Crystal structure of formate dehydrogenase FDH2 enzyme from Granu... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6t8c | ||||||
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Title | Crystal structure of formate dehydrogenase FDH2 enzyme from Granulicella mallensis MP5ACTX8 in the apo form. | ||||||
Components | Formate dehydrogenase | ||||||
Keywords | OXIDOREDUCTASE / formate dehydrogenase / NAD / NADP | ||||||
Function / homology | Function and homology information formate catabolic process / formate dehydrogenase / formate dehydrogenase (NAD+) activity / oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor / NAD binding / cytosol Similarity search - Function | ||||||
Biological species | Granulicella mallensis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.97 Å | ||||||
Authors | Robescu, M.S. / Rubini, R. / Filippini, F. / Bergantino, B. / Cendron, L. | ||||||
Citation | Journal: Chemcatchem / Year: 2020 Title: From the Amelioration of a NADP+-dependent Formate Dehydrogenase to the Discovery of a New Enzyme: Round Trip from Theory to Practice Authors: Robescu, M.S. / Rubini, R. / Beneventi, E. / Tavanti, M. / Lonigro, C. / Zito, F. / Filippini, F. / Cendron, L. / Bergantino, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6t8c.cif.gz | 306.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6t8c.ent.gz | 248.9 KB | Display | PDB format |
PDBx/mmJSON format | 6t8c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6t8c_validation.pdf.gz | 414.2 KB | Display | wwPDB validaton report |
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Full document | 6t8c_full_validation.pdf.gz | 419.1 KB | Display | |
Data in XML | 6t8c_validation.xml.gz | 28.3 KB | Display | |
Data in CIF | 6t8c_validation.cif.gz | 48.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t8/6t8c ftp://data.pdbj.org/pub/pdb/validation_reports/t8/6t8c | HTTPS FTP |
-Related structure data
Related structure data | 6t9wC 6t9xC 6tb6C 4xygS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 42589.566 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: aa not visible in the electron density maps are missing in the coordinates file Source: (gene. exp.) Granulicella mallensis (strain ATCC BAA-1857 / DSM 23137 / MP5ACTX8) (bacteria) Strain: ATCC BAA-1857 / DSM 23137 / MP5ACTX8 / Gene: AciX8_0868 / Production host: Escherichia coli (E. coli) / References: UniProt: G8NTI5, formate dehydrogenase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.58 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.04 M Potassium phosphate monobasic, 16 % w/v PEG 8000, 20 % Glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.9677 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jun 6, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9677 Å / Relative weight: 1 |
Reflection | Resolution: 1.97→48.95 Å / Num. obs: 636455 / % possible obs: 99.79 % / Redundancy: 5.5 % / CC1/2: 0.997 / Rmerge(I) obs: 0.096 / Rpim(I) all: 0.0453 / Net I/σ(I): 13.96 |
Reflection shell | Resolution: 1.97→2.04 Å / Rmerge(I) obs: 0.6385 / Mean I/σ(I) obs: 3.23 / Num. unique obs: 61436 / CC1/2: 0.71 / Rpim(I) all: 0.3057 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4xyg Resolution: 1.97→48.95 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso max: 91.13 Å2 / Biso mean: 22.7362 Å2 / Biso min: 11 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.97→48.95 Å
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