The biological unit is a dimer with two biological units in the asymmetric unit (biological unit 1, chains A & B) and (biological unit 2, chains C & D)
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Components
#1: Protein
Glyoxylatereductase/hydroxypyruvatereductase
Mass: 35901.562 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GRHPR, GLXR / Plasmid: PVP 16 / Production host: Escherichia coli (E. coli) / Strain (production host): BL834 P(RARE2) / References: UniProt: Q9UBQ7, glyoxylate reductase (NADP+)
Mass: 18.015 Da / Num. of mol.: 450 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.58 Å3/Da / Density % sol: 52.41 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.050 M SODIUM CHLORIDE, 0.0003 M TCEP, 0.005 MES PH 6.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (12% PEG 3350, 0.10 M MOPS PH 7). Crystal was cryo- ...Details: PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.050 M SODIUM CHLORIDE, 0.0003 M TCEP, 0.005 MES PH 6.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (12% PEG 3350, 0.10 M MOPS PH 7). Crystal was cryo-protected with 15% PEG 3350, 0.10 M PIPES PH 6.5 and a final concentration of 25% Ethylene glycol, vapor diffusion, hanging drop, temperature 293K
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