[English] 日本語
Yorodumi
- PDB-5cd4: The Type IE CRISPR Cascade complex from E. coli, with two assembl... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 5cd4
TitleThe Type IE CRISPR Cascade complex from E. coli, with two assemblies in the asymmetric unit arranged back-to-back
Components
  • (CRISPR system Cascade subunit ...) x 5
  • crRNA
KeywordsHYDROLASE/RNA / CRISPR / Cascade / RNA Surveillance / Adaptive Immunity / HYDROLASE-RNA complex
Function / homology
Function and homology information


CRISPR-cas system / DNA/RNA hybrid binding / maintenance of CRISPR repeat elements / RNA processing / RNA endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / nucleic acid binding / protein-containing complex / DNA binding ...CRISPR-cas system / DNA/RNA hybrid binding / maintenance of CRISPR repeat elements / RNA processing / RNA endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / nucleic acid binding / protein-containing complex / DNA binding / RNA binding / zinc ion binding
Similarity search - Function
CRISPR-associated protein Cse2 / Crispr-associated protein; domain 1 / Crispr-associated protein; domain 2 / Alpha-Beta Plaits - #2660 / CRISPR-associated protein, CT1975 / CT1975-like protein / CRISPR-associated protein, CasD / CRISPR-associated protein Cse2 / Cse2 superfamily / CRISPR-associated protein Cse2 (CRISPR_cse2) ...CRISPR-associated protein Cse2 / Crispr-associated protein; domain 1 / Crispr-associated protein; domain 2 / Alpha-Beta Plaits - #2660 / CRISPR-associated protein, CT1975 / CT1975-like protein / CRISPR-associated protein, CasD / CRISPR-associated protein Cse2 / Cse2 superfamily / CRISPR-associated protein Cse2 (CRISPR_cse2) / CRISPR-associated protein Cse1 / CRISPR-associated protein Cse1 (CRISPR_cse1) / CRISPR-associated protein Cse3 / CRISPR associated protein / CRISPR_assoc / CRISPR-associated protein, Cas5 / CRISPR-associated protein (Cas_Cas5) / CRISPR-associated protein Cas5, N-terminal / Peroxidase; domain 1 / Alpha-Beta Plaits / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
RNA / RNA (> 10) / CRISPR system Cascade subunit CasB / CRISPR system Cascade subunit CasE / CRISPR system Cascade subunit CasD / CRISPR system Cascade subunit CasC / CRISPR system Cascade subunit CasA
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å
AuthorsJackson, R.N. / Golden, S.M. / Carter, J. / Wiedenheft, B.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM108888 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)F32 GM108436 United States
CitationJournal: Nucleic Acids Res. / Year: 2015
Title: Mechanism of CRISPR-RNA guided recognition of DNA targets in Escherichia coli.
Authors: van Erp, P.B. / Jackson, R.N. / Carter, J. / Golden, S.M. / Bailey, S. / Wiedenheft, B.
History
DepositionJul 3, 2015Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 26, 2015Provider: repository / Type: Initial release
Revision 1.1Oct 28, 2015Group: Database references
Revision 1.2Jun 1, 2016Group: Data collection
Revision 1.3Sep 20, 2017Group: Author supporting evidence / Derived calculations / Category: pdbx_audit_support / pdbx_struct_oper_list
Item: _pdbx_audit_support.funding_organization / _pdbx_struct_oper_list.symmetry_operation
Revision 1.4Dec 25, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.5Sep 27, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_value_order / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

-
Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
A: CRISPR system Cascade subunit CasE
B: CRISPR system Cascade subunit CasC
C: CRISPR system Cascade subunit CasC
D: CRISPR system Cascade subunit CasC
E: CRISPR system Cascade subunit CasC
F: CRISPR system Cascade subunit CasC
G: CRISPR system Cascade subunit CasC
H: CRISPR system Cascade subunit CasD
I: CRISPR system Cascade subunit CasA
J: CRISPR system Cascade subunit CasB
K: CRISPR system Cascade subunit CasB
L: crRNA
M: CRISPR system Cascade subunit CasE
N: CRISPR system Cascade subunit CasC
O: CRISPR system Cascade subunit CasC
P: CRISPR system Cascade subunit CasC
Q: CRISPR system Cascade subunit CasC
R: CRISPR system Cascade subunit CasC
S: CRISPR system Cascade subunit CasC
T: CRISPR system Cascade subunit CasD
U: CRISPR system Cascade subunit CasA
V: CRISPR system Cascade subunit CasB
W: CRISPR system Cascade subunit CasB
X: crRNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)804,32526
Polymers804,19424
Non-polymers1312
Water0
1
A: CRISPR system Cascade subunit CasE
B: CRISPR system Cascade subunit CasC
C: CRISPR system Cascade subunit CasC
D: CRISPR system Cascade subunit CasC
E: CRISPR system Cascade subunit CasC
F: CRISPR system Cascade subunit CasC
G: CRISPR system Cascade subunit CasC
H: CRISPR system Cascade subunit CasD
I: CRISPR system Cascade subunit CasA
J: CRISPR system Cascade subunit CasB
K: CRISPR system Cascade subunit CasB
L: crRNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)402,16213
Polymers402,09712
Non-polymers651
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area55210 Å2
ΔGint-327 kcal/mol
Surface area128550 Å2
MethodPISA
2
M: CRISPR system Cascade subunit CasE
N: CRISPR system Cascade subunit CasC
O: CRISPR system Cascade subunit CasC
P: CRISPR system Cascade subunit CasC
Q: CRISPR system Cascade subunit CasC
R: CRISPR system Cascade subunit CasC
S: CRISPR system Cascade subunit CasC
T: CRISPR system Cascade subunit CasD
U: CRISPR system Cascade subunit CasA
V: CRISPR system Cascade subunit CasB
W: CRISPR system Cascade subunit CasB
X: crRNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)402,16213
Polymers402,09712
Non-polymers651
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area53600 Å2
ΔGint-297 kcal/mol
Surface area127700 Å2
MethodPISA
Unit cell
Length a, b, c (Å)105.990, 244.800, 426.740
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

-
Components

-
CRISPR system Cascade subunit ... , 5 types, 22 molecules AMBCDEFGNOPQRSHTIUJKVW

#1: Protein CRISPR system Cascade subunit CasE / CasE endoRNase / crRNA endonuclease


Mass: 22324.002 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: casE, cas6e, ygcH, b2756, JW2726 / Production host: Escherichia coli (E. coli)
References: UniProt: Q46897, Hydrolases; Acting on ester bonds
#2: Protein
CRISPR system Cascade subunit CasC


Mass: 40071.402 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: casC, cas4, cse4, ygcJ, b2758, JW2728 / Production host: Escherichia coli (E. coli) / References: UniProt: Q46899
#3: Protein CRISPR system Cascade subunit CasD


Mass: 25239.803 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: casD, cas5, ygcI, b2757, JW5844 / Production host: Escherichia coli (E. coli) / References: UniProt: Q46898
#4: Protein CRISPR system Cascade subunit CasA / CRISPR type I-E/Ecoli-associated protein CasA/Cse1 / CRISPR-associated protein CasA/Cse1


Mass: 55964.957 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: casA, cse1, ygcL, b2760, JW2730 / Production host: Escherichia coli (E. coli) / References: UniProt: Q46901
#5: Protein
CRISPR system Cascade subunit CasB


Mass: 19231.049 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: casB, cse2, ygcK, b2759, JW2729 / Production host: Escherichia coli (E. coli) / References: UniProt: P76632

-
RNA chain / Non-polymers , 2 types, 4 molecules LX

#6: RNA chain crRNA


Mass: 19677.646 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K-12 / Production host: Escherichia coli (E. coli)
#7: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION

-
Sample preparation

CrystalDensity Matthews: 3.44 Å3/Da / Density % sol: 64.27 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop
Details: 0.1 M HEPES pH 7.0, 0-0.1 M KCl, and 8-14 percent (w/v) PEG 8000. The crystal used in this study grew in the presence of an 11-nt ssDNA containing a 5-CTT-3 PAM and nine nucleotides ...Details: 0.1 M HEPES pH 7.0, 0-0.1 M KCl, and 8-14 percent (w/v) PEG 8000. The crystal used in this study grew in the presence of an 11-nt ssDNA containing a 5-CTT-3 PAM and nine nucleotides complementary to the crRNA-guide sequence in a 2:1 oligonucleotide:protein ratio. However, the target DNA is not observed in the electron
PH range: 6.5-7.5

-
Data collection

DiffractionMean temperature: 80 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1 Å
DetectorType: MAR scanner 300 mm plate / Detector: IMAGE PLATE / Date: Mar 22, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.2→49.74 Å / Num. obs: 183649 / % possible obs: 100 % / Observed criterion σ(I): 2 / Redundancy: 7.3 % / Net I/σ(I): 8
Reflection shellResolution: 3.2→3.25 Å / Redundancy: 6.9 % / Mean I/σ(I) obs: 2 / % possible all: 100

-
Processing

Software
NameVersionClassification
PHENIX(1.10pre_2089: ???)refinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1VY8

1vy8
PDB Unreleased entry


Resolution: 3.2→49.735 Å / SU ML: 0.44 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 24.47 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2496 2000 1.09 %
Rwork0.2122 --
obs0.2127 183492 99.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.2→49.735 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms50706 2600 2 0 53308
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00454557
X-RAY DIFFRACTIONf_angle_d0.7474581
X-RAY DIFFRACTIONf_dihedral_angle_d11.45932412
X-RAY DIFFRACTIONf_chiral_restr0.0348588
X-RAY DIFFRACTIONf_plane_restr0.0039271
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.2-3.280.32691410.303312803X-RAY DIFFRACTION100
3.28-3.36870.33061420.28212843X-RAY DIFFRACTION100
3.3687-3.46780.29231410.270112818X-RAY DIFFRACTION100
3.4678-3.57970.33511410.255412835X-RAY DIFFRACTION100
3.5797-3.70760.28271420.236412873X-RAY DIFFRACTION100
3.7076-3.8560.28611420.230412883X-RAY DIFFRACTION100
3.856-4.03140.30161410.215212868X-RAY DIFFRACTION100
4.0314-4.24380.22991430.198412943X-RAY DIFFRACTION100
4.2438-4.50960.22131430.184912895X-RAY DIFFRACTION100
4.5096-4.85750.22741420.173412953X-RAY DIFFRACTION100
4.8575-5.34580.19721430.180213004X-RAY DIFFRACTION100
5.3458-6.11810.23231440.203613056X-RAY DIFFRACTION100
6.1181-7.70360.22591460.212813152X-RAY DIFFRACTION100
7.7036-49.7410.1941490.175213566X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.1270.51961.20130.44341.06713.64110.75070.518-0.828-0.7445-0.0396-0.08420.7437-0.2728-0.46911.0667-0.02560.01330.5619-0.03151.3689-83.4375.5641-95.3854
21.3685-0.7454-0.13893.8155-0.60652.5797-0.1790.1488-0.8363-0.02640.13080.7840.7763-0.3524-0.02150.7367-0.074-0.07310.4830.01720.9879-88.936583.7691-89.7468
32.20630.2718-0.44052.5126-0.60485.05160.28470.24510.4804-0.160.21510.0776-0.6043-0.6692-0.23170.46960.0623-0.13440.41030.09340.5037-81.6989100.5849-100.1403
48.1134-1.1527-2.46622.96950.62964.9551-0.1330.2365-0.7183-0.46220.01950.62170.6984-0.32950.1230.5763-0.0579-0.15060.35890.04650.5549-86.736391.2205-93.7158
52.38460.54450.17650.4922-0.79922.0066-0.04720.15450.22270.20510.0860.0854-0.2626-0.11070.02670.54650.17030.04990.30830.02220.4373-72.2703107.1939-71.641
65.9836-0.9372-2.48025.87770.78735.1513-0.39390.89560.2898-0.9008-0.3852-0.3073-1.56140.5310.23620.9642-0.29470.07480.7608-0.00810.6517-50.0368120.5472-79.3449
71.88510.10180.1042.31540.55923.1083-0.25990.5181-0.0294-0.46040.342-0.9765-1.16151.6916-0.15720.9451-0.35720.07971.0107-0.16731.3101-39.5313120.8016-72.4709
80.82271.1691.38413.44640.95365.0605-0.15420.20620.38920.62820.5512-0.8297-0.73030.9109-0.27530.7367-0.0673-0.08690.4683-0.07920.7287-51.6374115.7781-63.6722
93.56871.23460.83542.51171.3582.9146-0.2709-0.161-0.51580.12630.20430.38540.4065-0.9860.02180.6018-0.09610.02550.58530.21540.6806-90.03494.2018-76.2328
106.55211.54772.54461.57610.42192.7181-0.3256-0.15930.76160.3479-0.01250.1663-0.790.05780.38240.75450.17630.00080.3976-0.03360.4947-67.764117.2829-60.5549
112.03470.47271.35082.72711.70656.3289-0.52090.06010.8575-0.3561-0.23420.4857-0.321-0.78620.41870.64340.2639-0.08010.5720.09960.7223-81.0464115.1429-68.5904
121.56180.6096-0.25824.58720.24412.6054-0.049-0.64050.10850.40210.17770.5065-0.4697-0.7758-0.00630.55590.31120.09610.63580.03180.5543-83.9183105.1206-56.9805
136.08811.7328-0.24393.4982-0.00683.3056-0.2073-0.39050.58830.18360.10140.632-0.5803-0.64280.10340.97920.42090.14360.73120.09370.7304-81.6866120.1434-54.8798
141.81760.54950.4832.00720.00752.26660.1435-0.15840.25250.43170.0412-0.4102-0.35370.4182-0.18220.650.1227-0.03020.4967-0.0650.4338-49.111594.2139-45.5827
151.3575-0.23380.66661.2693-0.57612.357-0.1293-0.20640.19430.47540.0869-0.1823-0.23680.2935-0.01470.57110.1486-0.05540.4297-0.080.3488-55.950890.6102-45.8055
161.42930.2786-0.82911.187-1.66514.46830.0459-0.24860.28440.49750.33160.4962-0.20220.2879-0.15830.55120.19170.09160.3204-0.05960.3725-68.954795.4567-52.5152
170.64650.88940.24123.1298-1.41674.2262-0.313-0.35510.570.30220.18330.07510.1573-0.8528-0.15880.40220.13560.10660.4990.03710.3966-78.294878.9005-50.6679
182.01490.52751.62063.56662.2052.3080.1741-0.56070.26810.343-0.30660.63160.1207-0.80560.29730.75290.29780.31720.7081-0.00940.5251-79.118490.2616-37.7226
191.95240.310.07232.34340.22.21680.0431-0.68220.00270.79680.0209-0.3428-0.18240.2229-0.08410.45690.0832-0.04240.5632-0.0070.3551-50.235561.5324-37.6177
201.7311-0.03050.441.7143-0.3680.5084-0.2763-0.7385-0.27691.0059-0.0386-0.33910.31080.48370.15120.84570.2411-0.07080.91910.19310.496-47.673147.627-26.5452
211.61842.0613-0.28824.22310.06420.3358-0.10170.26230.3291-0.12590.26850.2236-0.14430.360.05150.27710.1318-0.0010.4595-0.00230.2434-58.078772.3026-59.8869
222.18120.44910.10163.77911.47882.9887-0.1241-0.00210.5802-0.44060.2591-0.7952-0.82070.2989-0.15210.76360.2537-0.01790.4126-0.05620.6277-48.00489.7966-56.7727
231.4578-0.4109-0.02273.7156-0.96471.4291-0.1837-0.3893-0.28560.6496-0.0834-0.1515-0.1903-0.27910.11910.35590.11250.10720.56970.06090.3264-65.439353.7538-38.2438
241.10520.1352-0.34160.9425-0.11810.84710.0441-0.30490.00630.3837-0.18490.321-0.0915-0.4325-0.01480.28480.11150.15460.3984-0.02860.3785-66.350862.8853-48.1612
250.90731.3264-1.28113.9216-0.50332.6752-0.2843-0.0565-0.06450.01370.44780.7514-0.1939-0.4937-0.29820.21750.06780.04730.43820.13810.5872-74.678458.3276-60.4005
261.5614-1.0871-1.52813.1642.04112.7921-0.0199-0.1067-0.24190.46910.23380.75970.1468-0.5334-0.03410.39470.18170.25490.62560.16120.6196-79.077255.5287-42.8125
270.50820.028-0.06082.5187-0.12570.7662-0.0456-0.2341-0.18240.6284-0.0362-0.11780.16860.05740.04780.27330.0821-0.02310.45870.13690.3824-49.2324.2299-50.2641
281.6490.8098-0.21874.6573-0.93492.3515-0.2758-0.0483-0.04410.04360.0803-0.70210.36170.28180.15390.2640.01830.06420.38460.12080.4186-51.09417.0405-55.883
291.1858-0.2865-0.15982.20070.17931.02350.0183-0.12070.11950.0605-0.0621-0.2355-0.2033-0.0412-0.0320.0810.05940.02990.32350.11630.2754-51.758447.6709-58.3229
300.77940.44630.46034.4114-0.0150.53140.2456-0.5964-0.5730.5926-0.13140.49050.0307-0.29140.19940.2617-0.04660.17120.38620.15360.7282-66.469824.9504-58.4901
311.94550.38510.44741.3630.74471.6423-0.10010.1879-0.0084-0.13840.05230.4359-0.0787-0.17630.11960.13340.07880.010.31650.05740.4291-62.605542.3498-70.1127
326.1808-2.16850.11883.3699-0.06344.63060.21020.4483-0.1472-0.22-0.11560.69140.1509-0.80870.22440.13350.04410.06740.44930.04840.6957-74.786534.2609-68.9349
330.3975-0.1151-0.27241.01660.16421.20570.03190.0193-0.2094-0.15870.01420.05710.0440.0063-0.01850.0214-0.0355-0.01330.30580.10830.2943-43.518928.2271-82.5185
341.1941-0.37980.35993.04321.14582.58580.1185-0.5605-0.57850.97470.03180.49781.3427-0.7127-0.33420.6669-0.0673-0.01140.42750.05050.5372-43.04871.7705-75.9123
352.252-0.47051.19412.9029-0.06754.7888-0.0022-0.2433-0.41530.5030.0458-0.33741.2465-0.03190.0260.5228-0.0193-0.0590.34920.02130.6215-39.4607-4.1283-86.092
361.3946-3.0667-0.43598.32960.7521.561-0.2880.31030.1012-0.1220.0813-0.8350.17280.4250.11760.3094-0.02260.01710.4372-0.01690.5025-35.387.9463-90.5654
370.91880.1595-0.45151.5421-0.62063.114-0.0508-0.20830.10580.3333-0.0783-0.1176-0.07070.53570.03180.14070.0648-0.06270.35330.02260.309-41.077537.3085-64.2643
380.9695-0.09080.23520.72950.41531.2290.03250.1752-0.1162-0.054-0.11540.222-0.1484-0.30210.04990.0618-0.0008-0.03180.3752-0.00810.4333-48.754727.7107-89.8726
392.00810.2843-2.09441.39661.06933.54280.21920.196-0.1422-0.3539-0.15450.7471-0.3584-0.31810.40470.2020.0774-0.22510.33180.03060.392-47.867241.6366-98.3152
407.04650.6804-4.96350.433-0.83538.76830.0980.75730.3074-0.15110.35120.3493-0.689-0.8421-0.18870.19340.015-0.11530.52420.01320.5159-58.874131.8314-98.5986
411.84190.1963-0.65411.37810.07110.4681-0.0051-0.0479-0.3703-0.04950.1041-0.03870.1651-0.0271-0.06730.1035-0.0615-0.01670.25670.02490.2025-24.583530.2714-101.3029
422.64550.0543-1.25522.37730.61132.7483-0.33780.3351-0.2067-0.66360.3095-0.4270.13150.0449-0.08410.5417-0.13370.05550.6306-0.23870.4827-16.77818.282-131.3163
430.8556-0.10820.10291.18460.78991.4526-0.06960.405-0.2023-0.19170.4291-0.1072-0.11860.0057-0.12450.0561-0.27740.04930.4444-0.1130.3274-19.325224.5656-110.3857
443.79113.3234-1.07886.8537-0.95692.21760.1612-0.68330.00220.7304-0.2358-0.06260.09450.00420.010.14780.0335-0.0180.35980.03320.3867-32.708720.7746-76.7333
451.33740.0002-0.22730.82040.09413.03810.01740.26790.2051-0.17510.13920.2137-0.2965-0.1133-0.10680.15650.0023-0.02330.36580.05970.2174-26.632438.2919-108.5078
466.68782.56981.43793.82921.61615.08510.02620.45350.8803-0.36880.44020.3403-0.7932-0.2443-0.08850.34560.0548-0.12880.4867-0.00260.5137-32.713145.5608-115.9636
470.8143-0.53610.24650.9254-0.15930.8869-0.08260.0279-0.0146-0.14610.276-0.4623-0.1820.19070.20990.1541-0.45570.04670.59610.07360.3905-1.227440.1893-109.8991
480.79710.1234-0.38350.635-0.19470.35180.1312-0.14010.02040.00120.0325-0.1297-0.08920.00850.04980.1536-0.1824-0.06090.5956-0.1820.5596-1.718125.9622-101.4086
493.46881.14260.87021.5717-0.00981.22440.20490.3212-0.7964-0.16340.1282-0.5951-0.34060.617-0.16360.2577-0.21250.02630.6966-0.1250.4550.303232.6235-118.0399
503.63963.148-0.18167.36420.29932.34870.1812-0.24740.45360.5460.0495-0.1222-0.53840.1924-0.19310.2185-0.0163-0.05940.4706-0.07870.4055-10.522436.6805-95.2519
515.3241.77191.07575.13331.60364.2722-0.36890.5888-1.3284-0.13110.0369-0.58030.60840.0914-0.06050.2727-0.03770.02970.4941-0.21420.76-15.02146.6523-108.8358
521.93852.346-0.48763.7459-1.41681.2228-0.1390.2439-0.101-0.0477-0.0782-0.1509-0.04750.2617-0.00890.16250.00650.05630.4393-0.05290.3722-5.825432.0945-103.5743
538.6824-0.5821-0.52351.3756-0.18570.0691-0.14121.30870.1108-0.72040.3945-0.14220.01870.60490.05790.4182-0.16190.0810.9246-0.01320.36911.147140.7706-125.0179
542.33990.36250.80114.192-0.80510.96010.05480.75240.267-0.12310.16440.4079-0.31160.41440.06520.2112-0.12290.07020.4144-0.00930.4251-11.762235.9287-113.5697
550.9837-0.14021.17211.107-0.36071.41380.1268-0.2810.70910.0187-0.2476-0.5732-0.5120.5830.22760.371-0.30370.19260.6627-0.0270.72114.75249.4376-110.208
563.83641.9443-0.34566.47940.75181.46690.267-0.18160.05220.05920.1934-0.7676-0.6080.7293-0.01730.3457-0.22140.02780.9113-0.0660.473712.496535.0992-106.7595
572.81851.61860.70022.51810.75490.96930.059-0.152-0.12140.0639-0.1744-0.2055-0.2350.00680.14710.026-0.17780.04320.8439-0.26330.6177.882630.0701-108.8138
580.6746-0.54080.01870.5702-0.37071.1716-0.18580.7727-0.22840.14360.1053-0.3719-0.15120.50940.05640.19820.03440.00410.7435-0.29110.762227.313513.8329-104.1845
590.00710.0353-0.03960.2374-0.39550.652-0.21990.3465-1.1112-0.12090.283-0.0590.59050.13630.06820.4984-0.01620.03640.6848-0.40351.327613.8338-3.048-105.2612
600.6659-0.15690.29310.2053-0.09430.07960.03060.2854-0.80270.00220.0342-0.17780.2451-0.08950.01050.0423-0.1171-0.06130.6836-0.36940.98836.24447.3314-100.1137
612.03680.83770.07711.03560.95681.2525-0.094-0.018-1.10.3211-0.1711-0.22420.50380.05830.15120.256-0.05640.04640.4944-0.02441.087825.08034.2949-91.1495
621.69470.36980.57420.46620.37842.06180.07040.2482-0.88860.1264-0.293-0.01130.6494-0.19180.0510.1131-0.00020.03440.5367-0.28821.356113.2217-0.2151-99.667
631.995-0.59830.48231.41130.95121.1540.0799-0.5453-0.04260.5966-0.27340.24670.3454-0.1945-0.04150.0117-0.19440.07810.4958-0.05250.881315.231417.5785-79.8106
643.80392.15821.42391.72151.02850.8306-0.2755-0.0152-0.1058-0.19130.1432-0.1306-0.134-0.32760.00050.1995-0.0452-0.10770.4947-0.11170.628611.34421.0122-90.1576
652.1272-0.8529-1.09030.72080.43870.7143-0.1328-0.0333-0.69010.27280.0224-0.18160.2307-0.0002-0.11720.0832-0.0011-0.05980.44890.04990.4447-6.702929.3921-76.1305
665.57730.278-1.63660.5675-0.29990.50070.0783-0.4370.424-0.060.0805-0.0471-0.0060.2408-0.02720.1994-0.0483-0.01830.4233-0.05050.4861-2.513737.7523-76.6416
676.49442.05430.56045.8373-0.33173.26640.27090.6557-0.2873-1.4969-0.28670.3038-0.273-0.32250.10260.79070.3074-0.10240.6813-0.03510.5034-55.09383.7615-92.9207
682.12953.2618-0.44615.6270.52798.7195-0.27770.48380.5031-1.0086-0.5474-1.4792-0.55991.40330.10670.76080.07390.2680.79910.15910.7488-37.427691.1632-86.975
695.46121.14593.2077.4224-1.08332.3843-0.6379-0.4213-0.7945-0.00370.36710.3402-0.0793-0.3020.32890.54280.17090.20550.45940.05760.6254-47.479182.4776-74.537
708.33752.51213.30777.7586-4.75118.23720.0861.4818-0.2695-1.2209-0.2013-0.86230.56640.7265-0.00220.74680.23610.14650.58820.04150.5801-42.376981.8149-86.6183
715.55531.5451-1.38838.195-5.63784.59810.34880.1243-0.1246-0.0942-0.3677-1.46250.0491.21270.03030.41710.1590.11030.49960.10390.7677-36.23478.6092-75.0645
729.47890.0872-0.79356.98810.49595.50850.9186-0.48910.16330.5457-0.0745-0.7465-0.77221.5577-0.27550.5063-0.0964-0.23750.5942-0.14340.6409-20.168769.5792-63.6795
733.62690.953-0.25165.49762.33761.209-0.2333-1.28570.56631.11620.32060.239-0.3268-0.09550.00270.82720.26130.37790.5521-0.22830.6781-34.705171.657-57.7455
743.2018-0.94733.52513.6956-2.92984.93450.54540.0448-0.46890.10060.3330.85220.11540.01580.17980.42450.14760.18960.5425-0.03430.5567-36.013669.5191-68.3122
756.79751.41595.21965.02191.97764.40830.09320.5281.2296-0.6838-0.1462-0.0642-0.32740.8019-0.33060.5216-0.05650.34370.52520.03730.8829-24.336573.0932-74.8642
766.0227-2.92320.68215.6347-1.79561.85210.2821-0.56950.37490.69010.0009-0.5532-0.40180.4345-0.3220.3677-0.0024-0.1040.5071-0.05350.5677-23.451661.2889-62.3918
776.4493-0.1806-1.11527.15381.0863.5747-0.2207-0.05580.22310.32010.16790.18970.1463-0.13970.09310.1928-0.01030.0280.3873-0.03320.2134-29.994149.2182-63.8521
785.373-0.222-0.74222.4797-0.77363.6833-0.0777-0.10020.4664-0.05680.0974-0.9099-0.10520.3875-0.02310.2809-0.0352-0.07160.35570.03040.4124-20.138550.8054-66.0678
790.46850.1552-0.24280.327-0.3090.30090.00460.346-0.0145-0.3669-0.0773-0.0529-0.07930.20670.0433-0.2401-0.2888-0.13370.4183-0.20220.4325-13.85722.1314-106.4454
800.6756-0.9527-1.47361.41561.38874.1516-0.2213-0.1634-0.15630.30570.11370.00230.4310.39750.06750.20930.03110.06280.35240.01620.4932-38.962822.7442-71.3792
810.3385-0.13340.02452.1045-0.45020.0822-0.2189-0.04770.05130.3585-0.1203-0.7695-0.15570.14810.11790.45530.1144-0.06180.45270.03110.4405-48.803771.9798-50.36
824.5606-0.4195-2.98146.25541.70132.56530.4280.55550.3848-0.5325-0.51020.2802-0.44320.15280.03290.47760.0636-0.13510.3834-0.00880.5423-61.5381105.7164-77.7615
834.4981-2.09191.28847.0910.24775.03030.19290.2422-0.4546-0.43240.4118-0.17010.5530.4144-0.37870.67120.1013-0.11230.40670.00020.4516-73.874596.9732-104.8965
841.85511.0856-1.4164.6235-1.0842.6523-0.48750.68750.8895-1.88520.21720.2223-1.11190.84840.26471.1189-0.1142-0.19021.17010.33111.02-83.6403104.9175-116.7082
853.8026-1.5607-0.3815.9176-2.16352.6962-0.31330.95240.0816-0.02270.124-0.18520.28510.06080.29650.6649-0.107-0.21480.47750.00590.7312-85.074794.9609-107.8851
863.74390.196-0.51112.0529-1.35044.0896-0.0161.28250.8405-1.0341-0.2994-0.6070.02040.0250.33271.66660.28610.08151.320.31370.856628.5429-44.12885.1163
872.29930.2701-0.15374.58010.33521.8003-0.00050.0553-0.25320.2405-0.1568-0.3810.37720.76580.10211.480.4314-0.07940.89430.17470.642224.1983-55.985219.9556
882.0295-0.9473-1.03171.6140.0733.2551-0.6456-0.0556-0.35090.5544-0.09130.33590.9609-0.06960.59081.7570.20850.09190.49540.04740.64450.5923-41.924237.9595
893.3252-2.5239-1.98342.93053.62175.564-0.0048-0.3539-0.21590.42470.1931.224-0.0173-0.53390.26571.4972-0.09750.29870.83450.14830.8881-23.6445-47.148941.5214
900.52210.22620.10680.10190.1623.0366-0.4326-0.4725-0.43030.1698-0.16920.09090.0975-0.83660.33731.89440.09430.26990.5566-0.04770.9446-20.1082-35.985543.1376
910.8560.2903-0.42621.79172.55784.4546-0.0937-0.5505-0.2481-0.02020.6977-0.5475-0.19851.34970.79621.42240.0843-0.11910.55560.26360.847127.1565-35.813426.2837
921.14790.2805-0.59441.0783-0.52951.9051-0.3176-0.4692-0.13950.9446-0.1864-0.1189-0.17450.63770.30461.71290.3406-0.24480.61070.12340.6587.6138-31.017244.5718
930.9435-0.3291-0.05250.912-0.66991.7542-0.2632-0.51580.28030.70090.1644-0.3978-0.37350.88410.45951.72050.2375-0.46380.90460.04140.809714.7429-22.628348.8878
941.5529-0.3305-0.24641.44870.06812.2502-0.2562-0.2832-0.0390.4056-0.00350.31790.0218-0.0830.2191.18220.1551-0.10940.41130.02860.4901-8.7219-14.891526.6646
952.37130.27280.53682.9243-0.25190.89810.0869-0.17590.35790.3834-0.29630.4413-0.5914-0.79360.25151.27350.3001-0.09270.8092-0.24520.8165-28.0879-1.242728.8639
960.6077-1.14990.12312.3635-0.75951.1462-0.4668-0.3233-0.34310.1778-0.22990.12990.37480.2167-1.82971.36020.3497-0.08690.43460.16840.64580.3232-35.412423.6983
972.0594-0.60370.34234.158-1.46480.5245-0.4891-0.25060.5970.48750.28210.1958-0.4478-0.03180.20281.3120.124-0.31550.4697-0.06840.6205-3.7357-0.776529.7328
980.0848-0.04930.01720.1494-0.29060.9112-0.262-0.0360.33440.7058-0.0175-0.4473-0.41650.69660.56981.63620.1144-0.73780.42520.34310.5869.5357-6.572626.0475
990.8335-0.5362-0.4130.7624-0.37321.7819-0.3342-0.0030.16680.57390.0866-0.0501-0.19850.19740.08340.9650.0172-0.2710.42910.16390.5208-3.02-1.64110.8914
1001.01430.21980.35411.8051-0.23842.0435-0.0526-0.04360.6160.5436-0.24890.3721-0.8838-0.17090.08721.2180.1482-0.40930.38940.1080.91-11.660911.29750.5734
1011.14911.1748-0.36841.87660.97732.8653-0.3994-0.00210.0030.41270.1203-0.9281-0.4640.71560.310.8069-0.0816-0.37310.63820.33880.866914.8340.4538-4.1283
1020.9055-0.61180.00451.8874-0.07981.8121-0.12030.14730.17310.42160.0347-0.1286-0.07750.14690.01530.3935-0.0338-0.08220.50130.2460.4355-2.9827-3.9618-25.959
1031.8752-0.7369-0.77322.1269-1.16342.6609-0.1664-0.15350.51380.78870.2068-0.0717-0.7237-0.1897-0.11590.6316-0.0098-0.03490.4990.21740.554-13.475621.7808-39.6149
1040.26070.5175-0.88365.50790.02173.8269-0.06580.48270.2356-0.7319-0.2941-0.6248-0.68460.59560.37450.6508-0.0804-0.16340.69560.44730.6886-8.470722.3106-54.5335
1050.6364-0.651-0.23545.5184-3.00975.4537-0.32670.57570.43430.0192-0.0951-0.0969-0.29060.43850.38860.4943-0.045-0.06090.62710.27760.618-4.841711.1915-46.0418
1061.0360.5029-0.5541.2079-0.57123.3785-0.07210.11750.07730.1609-0.1349-0.3731-0.02510.34060.20650.40160.0485-0.15540.50770.23310.53131.227-6.4836-25.3764
1075.30782.37163.32923.35193.9625.8845-0.13310.46610.2720.28220.2745-1.05270.10371.0483-0.08730.40680.0889-0.09070.82090.28180.914418.3658-6.7599-31.2998
1080.58680.6176-0.37522.024-0.04252.5045-0.1690.28830.0618-0.23520.17170.0276-0.28710.0987-0.03180.3754-0.0083-0.01710.51830.1390.449-9.8278-12.4799-58.9499
1091.5979-0.2412-1.7424.1133-2.3817.6697-0.32750.4683-0.4786-0.5972-0.08970.0872-0.05770.20920.08780.5003-0.2133-0.01130.84840.08790.521-7.6787-16.3595-77.9895
1100.73410.22420.22081.4265-1.10651.5817-0.03960.176-0.01130.0605-0.08030.02970.5275-0.45750.32750.32950.0239-0.00880.46380.20460.4656-13.2515-20.1007-43.3275
1111.0958-0.11550.11342.28560.9362.7368-0.20680.23770.14940.2004-0.12240.26550.0957-0.26460.30530.26590.0271-0.02720.41290.2170.372-10.5927-13.6072-42.8765
1121.7961-0.46510.08452.7382.68364.071-0.16370.40510.0875-0.08610.0777-0.34060.04030.5973-0.02690.33440.0950.0360.65110.26790.45690.788-21.8547-50.2276
1131.86730.40850.03476.48433.99985.2444-0.2957-0.0825-0.60580.8090.5188-0.50891.33371.1287-0.24620.60680.17940.04870.50850.07740.6159-6.4618-36.7444-38.8948
1146.6154.36512.66727.98892.89712.3589-0.02490.0069-0.80570.3604-0.0678-1.1870.760.9829-0.03140.52520.2840.03940.80760.19120.76887.721-34.6686-47.5863
1151.07030.5280.34331.25230.3082.29160.07090.28340.0906-0.0619-0.16410.19530.19640.09720.06170.58480.06230.07960.29490.13920.4305-25.2151-40.07-54.7198
1161.22740.04230.9621.22830.25862.2670.28410.5699-0.7685-0.65810.27550.07990.99280.619-0.26451.56640.20840.03110.7547-0.11630.775-24.2222-63.8532-84.212
1171.80050.50440.86851.92730.02983.4988-0.10590.79460.2575-0.85040.0490.01720.16330.5278-0.0021.38640.09970.01250.63630.03260.5722-27.9861-53.4018-86.4221
1181.55720.79120.61162.81391.06363.6887-0.00820.3613-0.1772-0.50680.43670.47290.61910.3890.01380.86210.0340.21290.48690.05030.4377-30.6582-50.1556-72.5055
1194.26752.5908-2.06593.5095-1.31273.5910.2306-0.13350.56130.8092-0.11820.8738-0.41320.2673-0.41580.36440.0861-0.11430.37950.14610.3482-22.4736-20.5603-50.1977
1200.5051-0.1351-0.28183.54070.29241.5146-0.06870.1698-0.0054-0.04050.19140.44020.38930.22810.35860.56770.01580.22950.27580.40780.3548-25.222-42.2809-55.1913
1210.71810.09050.29362.82030.08810.9234-0.3329-0.0112-0.07340.07440.22760.13250.50870.36210.010.67610.13870.13840.46880.1310.4555-17.4105-48.2334-55.9038
1222.49812.2009-0.10485.31082.64133.10060.3178-0.00210.12631.0495-0.70880.21240.73920.08990.14790.55240.08470.08790.36630.20790.4375-29.6292-46.3984-40.5739
1232.8799-0.1067-1.09883.9417-1.09942.1622-0.1417-0.3422-0.63840.5384-0.0515-0.05880.6026-0.0102-0.07131.00890.17120.0080.32290.18020.6968-23.9718-53.6397-39.0372
1242.82280.7211.71265.74050.79783.51150.0858-0.3519-0.2260.36580.1009-0.48380.9643-0.077-0.16771.02770.36140.11340.57650.14170.772-14.2413-59.6578-46.6476
1251.5616-0.02740.37625.8916-3.24754.1112-0.1837-0.06560.07920.0371-0.11760.46010.7224-0.29190.23210.9114-0.04920.1960.38320.14160.6056-47.9055-54.2351-52.5038
1262.7957-0.36260.3864.5599-2.30664.5237-0.17780.3049-0.1673-0.5322-0.2070.28750.7329-0.08570.41090.7757-0.03150.02440.34690.12210.5896-45.0147-50.7271-63.7979
1274.2608-1.5869-5.03330.741.44927.2027-0.17720.5995-0.4890.04720.40380.28451.0883-0.4815-0.11380.913-0.10730.01290.4314-0.07470.936-51.3561-59.0536-60.6932
1287.462.7809-4.48153.7432-3.96964.71540.1963-1.0095-0.1950.7073-0.43690.3995-0.72170.2226-0.29340.8549-0.0310.15650.2680.06680.6941-41.4272-38.6065-47.7673
1292.48421.0755-1.46445.74680.74381.2488-0.33151.01430.4733-1.48690.3366-0.1878-0.4933-0.09990.05250.81450.00590.03030.68740.1350.641-30.566-37.9695-79.4498
1302.83443.2937-2.05324.4043-2.59112.2218-0.08650.1301-0.1292-0.31390.06720.46380.3866-0.12140.1590.65380.0410.07180.29270.05210.5323-44.2009-45.3588-56.1902
1316.9813.9736-0.90674.39030.00280.24730.232-0.2108-0.9395-0.4105-0.05150.32171.0388-0.1596-0.10031.232-0.06560.17680.38490.08150.843-48.7193-68.2185-58.4433
1324.70530.30642.84286.0295-3.85154.4267-0.04590.401-0.32670.0902-0.4611-0.00130.46930.26440.3040.64890.05680.22270.25420.09080.7491-36.5076-54.275-56.325
1333.54860.3118-0.01594.30490.03882.83310.0847-0.4485-0.22250.30220.17580.99030.5495-0.4825-0.32581.0307-0.18850.17370.48330.04761.0267-57.6516-57.2914-46.7265
1341.88810.33130.07213.61640.05682.2501-0.02380.1119-0.059-0.17670.00280.54990.01450.0405-0.13160.6789-0.0847-0.03610.40950.02481.1294-53.9355-49.4587-68.3511
1353.02860.53820.91475.09791.95876.98150.1544-0.2241-0.16880.4109-0.31071.12770.6474-0.42280.17540.7947-0.19030.11780.55040.08321.314-60.9501-49.4004-53.5186
1361.9244-0.344-0.29351.450.58352.91850.05190.0887-0.147-0.5081-0.08780.6954-0.026-0.54370.16640.7755-0.1403-0.23780.55130.07440.8875-64.4619-31.8377-79.04
1372.41790.40691.59191.0260.68212.2778-0.1125-0.05650.0397-0.0753-0.10730.44470.2388-0.23220.14820.4161-0.02390.02950.35940.12940.6123-49.2528-20.8652-45.279
1381.4148-0.03930.21720.3334-0.95962.76480.0674-0.1884-0.69830.69450.1382-0.17551.16740.0787-0.43031.81510.3608-0.1430.64610.13930.8633-5.9542-53.87538.5441
1391.93951.20791.91160.79671.09362.0871-0.35290.1658-0.19380.78170.3976-0.39780.58460.8715-0.06871.21710.7265-0.19131.4115-0.04250.89953.5664-43.48046.2156
1401.12311.89521.56446.47647.2828.7564-0.8231-0.4652-0.9170.43370.6809-0.45910.14150.51490.071.72060.6692-0.07971.3396-0.18490.69720.5537-54.8462-0.161
1413.98261.1770.08743.3634-0.06870.0050.00170.3769-0.4906-0.53210.0046-0.47930.2655-0.03870.01481.38310.2401-0.31310.80650.01330.7005-5.1969-46.53370.1793
1425.85361.0868-0.87962.2509-1.40973.19130.1097-0.2398-0.39250.18720.26770.52730.5068-0.5298-0.62221.7147-0.07430.02180.5730.25360.722-18.228-49.002310.5595
1437.2999-1.35280.01874.3665-1.25785.6085-0.36410.1150.11621.0090.4460.09520.02190.2283-0.21941.3199-0.0667-0.21890.42040.08620.5521-13.9824-35.45097.8716
1449.0302-2.44061.35558.118-0.79518.52670.36150.0467-0.4860.01990.30410.2450.282-0.8657-0.50191.0524-0.0035-0.14310.58380.11430.3761-19.5516-38.22580.6486
1457.966-1.45971.10474.6227-1.58657.57230.11610.10520.45060.8426-0.2161-0.1006-0.4536-0.4175-0.05881.0238-0.09180.02590.47090.07190.3389-29.8055-22.6951-3.3378
1462.267-1.94561.68041.6949-1.74024.75750.2473-0.1113-0.32390.172-0.10340.52160.356-0.7682-0.10910.65220.06370.05270.38460.02420.4902-35.6023-17.4181-13.8481
1471.47571.15480.28311.5571-0.58563.5502-0.10750.0423-0.11640.03770.0383-0.2375-0.1174-0.07170.06250.49740.1410.01770.33960.11570.3969-32.0369-15.3439-23.0509
1480.8578-0.55710.70560.3625-0.47890.8782-0.3129-0.0012-0.0172-0.49890.15630.53430.57820.4-0.02960.58940.01910.11710.33770.0530.461-34.0724-42.6558-65.8062
1490.52210.5820.72983.19141.87241.834-0.13480.0520.04-0.3485-0.14070.2278-0.234-0.20460.20820.36990.0236-0.06850.47770.23560.4153-14.7422-8.4403-48.0132
1500.0004-0.19020.00960.1878-0.0309-0.0026-0.2795-0.04950.45180.18240.37960.3541-0.097-0.7743-0.12211.05360.0864-0.25710.40360.14150.6534-14.4119-7.236.4592
1511.77430.9963-0.06270.65970.21652.9602-0.31740.3346-0.2732-0.2159-0.20960.3586-0.10080.4940.17441.28720.3265-0.11630.583-0.0690.6727-2.1933-43.588830.7166
1522.4234-0.80280.57911.8261-0.16282.21120.51970.6219-0.28770.0477-0.4013-0.04720.51170.4295-0.15971.69270.570.03350.78580.13390.635414.7043-65.154316.3229
1531.2613-0.14-0.3220.2280.49551.14110.12550.5977-0.3779-0.017-0.11950.0999-0.0087-0.17350.06821.62620.61370.06561.1780.23871.154526.3272-72.032318.4194
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 36 )
2X-RAY DIFFRACTION2chain 'A' and (resid 37 through 97 )
3X-RAY DIFFRACTION3chain 'A' and (resid 98 through 143 )
4X-RAY DIFFRACTION4chain 'A' and (resid 144 through 199 )
5X-RAY DIFFRACTION5chain 'B' and (resid 1 through 57 )
6X-RAY DIFFRACTION6chain 'B' and (resid 58 through 79 )
7X-RAY DIFFRACTION7chain 'B' and (resid 80 through 144 )
8X-RAY DIFFRACTION8chain 'B' and (resid 145 through 184 )
9X-RAY DIFFRACTION9chain 'B' and (resid 185 through 221 )
10X-RAY DIFFRACTION10chain 'B' and (resid 222 through 260 )
11X-RAY DIFFRACTION11chain 'B' and (resid 261 through 284 )
12X-RAY DIFFRACTION12chain 'B' and (resid 285 through 337 )
13X-RAY DIFFRACTION13chain 'B' and (resid 338 through 362 )
14X-RAY DIFFRACTION14chain 'C' and (resid 1 through 129 )
15X-RAY DIFFRACTION15chain 'C' and (resid 130 through 260 )
16X-RAY DIFFRACTION16chain 'C' and (resid 261 through 284 )
17X-RAY DIFFRACTION17chain 'C' and (resid 285 through 323 )
18X-RAY DIFFRACTION18chain 'C' and (resid 324 through 363 )
19X-RAY DIFFRACTION19chain 'D' and (resid 1 through 115 )
20X-RAY DIFFRACTION20chain 'D' and (resid 116 through 184 )
21X-RAY DIFFRACTION21chain 'D' and (resid 185 through 201 )
22X-RAY DIFFRACTION22chain 'D' and (resid 202 through 221 )
23X-RAY DIFFRACTION23chain 'D' and (resid 222 through 260 )
24X-RAY DIFFRACTION24chain 'D' and (resid 261 through 293 )
25X-RAY DIFFRACTION25chain 'D' and (resid 294 through 322 )
26X-RAY DIFFRACTION26chain 'D' and (resid 323 through 362 )
27X-RAY DIFFRACTION27chain 'E' and (resid 1 through 145 )
28X-RAY DIFFRACTION28chain 'E' and (resid 146 through 184 )
29X-RAY DIFFRACTION29chain 'E' and (resid 185 through 238 )
30X-RAY DIFFRACTION30chain 'E' and (resid 239 through 260 )
31X-RAY DIFFRACTION31chain 'E' and (resid 261 through 323 )
32X-RAY DIFFRACTION32chain 'E' and (resid 324 through 363 )
33X-RAY DIFFRACTION33chain 'F' and (resid 1 through 57 )
34X-RAY DIFFRACTION34chain 'F' and (resid 58 through 79 )
35X-RAY DIFFRACTION35chain 'F' and (resid 80 through 163 )
36X-RAY DIFFRACTION36chain 'F' and (resid 164 through 184 )
37X-RAY DIFFRACTION37chain 'F' and (resid 185 through 221 )
38X-RAY DIFFRACTION38chain 'F' and (resid 222 through 304 )
39X-RAY DIFFRACTION39chain 'F' and (resid 305 through 323 )
40X-RAY DIFFRACTION40chain 'F' and (resid 324 through 363 )
41X-RAY DIFFRACTION41chain 'G' and (resid 2 through 57 )
42X-RAY DIFFRACTION42chain 'G' and (resid 58 through 155 )
43X-RAY DIFFRACTION43chain 'G' and (resid 156 through 195 )
44X-RAY DIFFRACTION44chain 'G' and (resid 196 through 221 )
45X-RAY DIFFRACTION45chain 'G' and (resid 222 through 322 )
46X-RAY DIFFRACTION46chain 'G' and (resid 323 through 363 )
47X-RAY DIFFRACTION47chain 'H' and (resid 1 through 19 )
48X-RAY DIFFRACTION48chain 'H' and (resid 20 through 32 )
49X-RAY DIFFRACTION49chain 'H' and (resid 33 through 68 )
50X-RAY DIFFRACTION50chain 'H' and (resid 69 through 89 )
51X-RAY DIFFRACTION51chain 'H' and (resid 90 through 102 )
52X-RAY DIFFRACTION52chain 'H' and (resid 103 through 122 )
53X-RAY DIFFRACTION53chain 'H' and (resid 123 through 138 )
54X-RAY DIFFRACTION54chain 'H' and (resid 139 through 157 )
55X-RAY DIFFRACTION55chain 'H' and (resid 158 through 183 )
56X-RAY DIFFRACTION56chain 'H' and (resid 184 through 201 )
57X-RAY DIFFRACTION57chain 'H' and (resid 202 through 219 )
58X-RAY DIFFRACTION58chain 'I' and (resid 1 through 84 )
59X-RAY DIFFRACTION59chain 'I' and (resid 85 through 124 )
60X-RAY DIFFRACTION60chain 'I' and (resid 125 through 176 )
61X-RAY DIFFRACTION61chain 'I' and (resid 177 through 219 )
62X-RAY DIFFRACTION62chain 'I' and (resid 220 through 283 )
63X-RAY DIFFRACTION63chain 'I' and (resid 284 through 342 )
64X-RAY DIFFRACTION64chain 'I' and (resid 343 through 374 )
65X-RAY DIFFRACTION65chain 'I' and (resid 375 through 427 )
66X-RAY DIFFRACTION66chain 'I' and (resid 428 through 495 )
67X-RAY DIFFRACTION67chain 'J' and (resid 4 through 67 )
68X-RAY DIFFRACTION68chain 'J' and (resid 68 through 86 )
69X-RAY DIFFRACTION69chain 'J' and (resid 87 through 124 )
70X-RAY DIFFRACTION70chain 'J' and (resid 125 through 141 )
71X-RAY DIFFRACTION71chain 'J' and (resid 142 through 159 )
72X-RAY DIFFRACTION72chain 'K' and (resid 3 through 16 )
73X-RAY DIFFRACTION73chain 'K' and (resid 17 through 26 )
74X-RAY DIFFRACTION74chain 'K' and (resid 27 through 44 )
75X-RAY DIFFRACTION75chain 'K' and (resid 45 through 54 )
76X-RAY DIFFRACTION76chain 'K' and (resid 55 through 86 )
77X-RAY DIFFRACTION77chain 'K' and (resid 87 through 124 )
78X-RAY DIFFRACTION78chain 'K' and (resid 125 through 160 )
79X-RAY DIFFRACTION79chain 'L' and (resid 1 through 10 )
80X-RAY DIFFRACTION80chain 'L' and (resid 11 through 20 )
81X-RAY DIFFRACTION81chain 'L' and (resid 21 through 35 )
82X-RAY DIFFRACTION82chain 'L' and (resid 36 through 40 )
83X-RAY DIFFRACTION83chain 'L' and (resid 41 through 50 )
84X-RAY DIFFRACTION84chain 'L' and (resid 51 through 55 )
85X-RAY DIFFRACTION85chain 'L' and (resid 56 through 60 )
86X-RAY DIFFRACTION86chain 'M' and (resid 1 through 82 )
87X-RAY DIFFRACTION87chain 'M' and (resid 83 through 199 )
88X-RAY DIFFRACTION88chain 'N' and (resid 2 through 84 )
89X-RAY DIFFRACTION89chain 'N' and (resid 85 through 129 )
90X-RAY DIFFRACTION90chain 'N' and (resid 130 through 184 )
91X-RAY DIFFRACTION91chain 'N' and (resid 185 through 221 )
92X-RAY DIFFRACTION92chain 'N' and (resid 222 through 304 )
93X-RAY DIFFRACTION93chain 'N' and (resid 305 through 362 )
94X-RAY DIFFRACTION94chain 'O' and (resid 2 through 78 )
95X-RAY DIFFRACTION95chain 'O' and (resid 79 through 184 )
96X-RAY DIFFRACTION96chain 'O' and (resid 185 through 221 )
97X-RAY DIFFRACTION97chain 'O' and (resid 222 through 260 )
98X-RAY DIFFRACTION98chain 'O' and (resid 261 through 362 )
99X-RAY DIFFRACTION99chain 'P' and (resid 1 through 57 )
100X-RAY DIFFRACTION100chain 'P' and (resid 58 through 284 )
101X-RAY DIFFRACTION101chain 'P' and (resid 285 through 362 )
102X-RAY DIFFRACTION102chain 'Q' and (resid 1 through 57 )
103X-RAY DIFFRACTION103chain 'Q' and (resid 58 through 115 )
104X-RAY DIFFRACTION104chain 'Q' and (resid 116 through 145 )
105X-RAY DIFFRACTION105chain 'Q' and (resid 146 through 175 )
106X-RAY DIFFRACTION106chain 'Q' and (resid 176 through 322 )
107X-RAY DIFFRACTION107chain 'Q' and (resid 323 through 363 )
108X-RAY DIFFRACTION108chain 'R' and (resid 1 through 115 )
109X-RAY DIFFRACTION109chain 'R' and (resid 116 through 175 )
110X-RAY DIFFRACTION110chain 'R' and (resid 176 through 200 )
111X-RAY DIFFRACTION111chain 'R' and (resid 201 through 238 )
112X-RAY DIFFRACTION112chain 'R' and (resid 239 through 284 )
113X-RAY DIFFRACTION113chain 'R' and (resid 285 through 322 )
114X-RAY DIFFRACTION114chain 'R' and (resid 323 through 363 )
115X-RAY DIFFRACTION115chain 'S' and (resid 2 through 57 )
116X-RAY DIFFRACTION116chain 'S' and (resid 58 through 112 )
117X-RAY DIFFRACTION117chain 'S' and (resid 113 through 163 )
118X-RAY DIFFRACTION118chain 'S' and (resid 164 through 184 )
119X-RAY DIFFRACTION119chain 'S' and (resid 185 through 212 )
120X-RAY DIFFRACTION120chain 'S' and (resid 213 through 238 )
121X-RAY DIFFRACTION121chain 'S' and (resid 239 through 284 )
122X-RAY DIFFRACTION122chain 'S' and (resid 285 through 304 )
123X-RAY DIFFRACTION123chain 'S' and (resid 305 through 338 )
124X-RAY DIFFRACTION124chain 'S' and (resid 339 through 362 )
125X-RAY DIFFRACTION125chain 'T' and (resid 1 through 19 )
126X-RAY DIFFRACTION126chain 'T' and (resid 20 through 50 )
127X-RAY DIFFRACTION127chain 'T' and (resid 51 through 68 )
128X-RAY DIFFRACTION128chain 'T' and (resid 69 through 88 )
129X-RAY DIFFRACTION129chain 'T' and (resid 89 through 103 )
130X-RAY DIFFRACTION130chain 'T' and (resid 104 through 122 )
131X-RAY DIFFRACTION131chain 'T' and (resid 123 through 138 )
132X-RAY DIFFRACTION132chain 'T' and (resid 139 through 157 )
133X-RAY DIFFRACTION133chain 'T' and (resid 158 through 192 )
134X-RAY DIFFRACTION134chain 'T' and (resid 193 through 210 )
135X-RAY DIFFRACTION135chain 'T' and (resid 211 through 219 )
136X-RAY DIFFRACTION136chain 'U' and (resid 1 through 374 )
137X-RAY DIFFRACTION137chain 'U' and (resid 375 through 496 )
138X-RAY DIFFRACTION138chain 'V' and (resid 4 through 26 )
139X-RAY DIFFRACTION139chain 'V' and (resid 27 through 37 )
140X-RAY DIFFRACTION140chain 'V' and (resid 38 through 51 )
141X-RAY DIFFRACTION141chain 'V' and (resid 52 through 67 )
142X-RAY DIFFRACTION142chain 'V' and (resid 68 through 86 )
143X-RAY DIFFRACTION143chain 'V' and (resid 87 through 129 )
144X-RAY DIFFRACTION144chain 'V' and (resid 130 through 160 )
145X-RAY DIFFRACTION145chain 'W' and (resid 5 through 51 )
146X-RAY DIFFRACTION146chain 'W' and (resid 52 through 94 )
147X-RAY DIFFRACTION147chain 'W' and (resid 95 through 160 )
148X-RAY DIFFRACTION148chain 'X' and (resid 1 through 10 )
149X-RAY DIFFRACTION149chain 'X' and (resid 11 through 20 )
150X-RAY DIFFRACTION150chain 'X' and (resid 21 through 35 )
151X-RAY DIFFRACTION151chain 'X' and (resid 36 through 40 )
152X-RAY DIFFRACTION152chain 'X' and (resid 41 through 50 )
153X-RAY DIFFRACTION153chain 'X' and (resid 51 through 60 )

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more