|Entry||Database: EMDB / ID: 7037|
|Title||Cryo-EM structure of the dimeric FO region of yeast mitochondrial ATP synthase with C1 symmetry|
|Map data||Sharpened map of dimeric FO region of yeast mitochondrial ATP synthase|
|Sample||Yeast mitochondrial ATP synthase FO complex:|
|Source||Saccharomyces cerevisiae (baker's yeast)|
|Method||single particle reconstruction / cryo EM / 3.7 Å resolution|
|Authors||Guo H / Rubinstein JL|
|Citation||Journal: Science / Year: 2017|
Title: Atomic model for the dimeric F region of mitochondrial ATP synthase.
Authors: Hui Guo / Stephanie A Bueler / John L Rubinstein
Abstract: Mitochondrial adenosine triphosphate (ATP) synthase produces the majority of ATP in eukaryotic cells, and its dimerization is necessary to create the inner membrane folds, or cristae, characteristic ...Mitochondrial adenosine triphosphate (ATP) synthase produces the majority of ATP in eukaryotic cells, and its dimerization is necessary to create the inner membrane folds, or cristae, characteristic of mitochondria. Proton translocation through the membrane-embedded F region turns the rotor that drives ATP synthesis in the soluble F region. Although crystal structures of the F region have illustrated how this rotation leads to ATP synthesis, understanding how proton translocation produces the rotation has been impeded by the lack of an experimental atomic model for the F region. Using cryo-electron microscopy, we determined the structure of the dimeric F complex from at a resolution of 3.6 angstroms. The structure clarifies how the protons travel through the complex, how the complex dimerizes, and how the dimers bend the membrane to produce cristae.
|Date||Deposition: Sep 21, 2017 / Header (metadata) release: Nov 8, 2017 / Map release: Nov 8, 2017 / Last update: Dec 13, 2017|
|Structure viewer||EM map: |
Downloads & links
|File||emd_7037.map.gz (map file in CCP4 format, 131073 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.06 Å|
CCP4 map header:
-Entire Yeast mitochondrial ATP synthase FO complex
|Entire||Name: Yeast mitochondrial ATP synthase FO complex / Number of components: 1|
-Component #1: protein, Yeast mitochondrial ATP synthase FO complex
|Protein||Name: Yeast mitochondrial ATP synthase FO complex / Recombinant expression: No|
|Mass||Theoretical: 400 kDa|
|Source||Species: Saccharomyces cerevisiae (baker's yeast) / Strain: W303-1A|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||Specimen conc.: 5 mg/ml / pH: 7.4|
|Vitrification||Cryogen name: OTHER / Temperature: 277 K / Humidity: 100 % / Details: blot for 26 seconds before plunging.|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 71 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 130000.0 X (nominal), 47170.0 X (calibrated) / Cs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: 1000.0 - 2000.0 nm / Energy window: 0-20 eV|
|Specimen Holder||Model: FEI TITAN KRIOS AUTOGRID HOLDER|
|Camera||Detector: GATAN K2 QUANTUM (4k x 4k)|
|Image acquisition||Number of digital images: 3023|
|Processing||Method: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 238848|
|3D reconstruction||Software: cryoSPARC / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF|
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