modulation by symbiont of host process / positive regulation of growth / negative regulation of growth / rRNA catabolic process / mRNA catabolic process / RNA endonuclease activity / endonuclease activity / Hydrolases; Acting on ester bonds / negative regulation of translation / negative regulation of DNA-templated transcription / DNA binding Similarity search - Function
SH3 type barrels. - #110 / mRNA interferase PemK-like / PemK-like, MazF-like toxin of type II toxin-antitoxin system / Plasmid maintenance toxin/Cell growth inhibitor / SH3 type barrels. / Roll / Mainly Beta Similarity search - Domain/homology
Mass: 13516.365 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Gene: mazF3, MT1134 / Production host: Escherichia coli (E. coli) References: UniProt: P9WIH8, UniProt: P9WIH9*PLUS, Hydrolases; Acting on ester bonds
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 4.24 Å3/Da / Density % sol: 76 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.1 M Bis Tris propane pH 7.0, 1.0 M ammonium, 5mM spermidine and 20% glycerol