PDB-5aao: Crystal structure of fluorogen-activating designed ankyrin repeat...

Basic information

• Entry: Database: PDB / ID: 5aao
• Title: Crystal structure of fluorogen-activating designed ankyrin repeat protein (DARPin) dimer in complex with malachite green
• Components: FAD3210
• Keywords: FLUORESCENT PROTEIN / HOMODIMER / DESIGNED ANKYRIN REPEAT PROTEIN (DARPIN) / FLUOROGEN-ACTIVATION / MALACHITE GREEN
• Function / homology: Ankyrin repeat-containing domain / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Mainly Alpha / Chem-6FE
• Biological species: SYNTHETIC CONSTRUCT (others)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å
• Authors: Batyuk, A. / Schuetz, M. / Kummer, L. / Wu, Y. / Mittl, P. / Plueckthun, A.
• Citation: Journal: J. Mol. Biol. / Year: 2016; Title: Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools.; Authors: Schutz, M. / Batyuk, A. / Klenk, C. / Kummer, L. / de Picciotto, S. / Gulbakan, B. / Wu, Y. / Newby, G.A. / Zosel, F. / Schoppe, J. / Sedlak, E. / Mittl, P.R. / Zenobi, R. / Wittrup, K.D. / Pluckthun, A.

History

Deposition	Jul 27, 2015	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Feb 3, 2016	Provider: repository / Type: Initial release
Revision 1.1	Mar 22, 2017	Group: Database references
Revision 1.2	Jan 10, 2024	Group: Data collection / Database references / Derived calculations / Other / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_initial_refinement_model / struct_site Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

Assembly

Deposited unit

A: FAD3210

B: FAD3210

C: FAD3210

D: FAD3210

E: FAD3210

F: FAD3210

G: FAD3210

H: FAD3210

I: FAD3210

J: FAD3210

K: FAD3210

L: FAD3210

hetero molecules

Summary:

• 225 kDa, 12 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	224,784	18
Polymers	222,621	12
Non-polymers	2,163	6
Water	6,107	339

1

A: FAD3210

I: FAD3210

hetero molecules

Summary:

• defined by author&software
• 37.5 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	37,464	3
Polymers	37,104	2
Non-polymers	360	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2210 Å2
ΔGint	-2.5 kcal/mol
Surface area	16750 Å2
Method	PQS

2

B: FAD3210

C: FAD3210

hetero molecules

Summary:

• defined by author&software
• 37.5 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	37,464	3
Polymers	37,104	2
Non-polymers	360	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2060 Å2
ΔGint	-13.5 kcal/mol
Surface area	16210 Å2
Method	PQS

3

D: FAD3210

E: FAD3210

hetero molecules

Summary:

• defined by author&software
• 37.5 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	37,464	3
Polymers	37,104	2
Non-polymers	360	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1820 Å2
ΔGint	-16.5 kcal/mol
Surface area	16610 Å2
Method	PQS

4

F: FAD3210

G: FAD3210

hetero molecules

Summary:

• defined by author&software
• 37.5 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	37,464	3
Polymers	37,104	2
Non-polymers	360	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1870 Å2
ΔGint	-6.5 kcal/mol
Surface area	16340 Å2
Method	PQS

5

H: FAD3210

J: FAD3210

hetero molecules

Summary:

• defined by author&software
• 37.5 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	37,464	3
Polymers	37,104	2
Non-polymers	360	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2050 Å2
ΔGint	-16.1 kcal/mol
Surface area	16630 Å2
Method	PQS

6

K: FAD3210

L: FAD3210

hetero molecules

Summary:

• defined by author&software
• 37.5 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	37,464	3
Polymers	37,104	2
Non-polymers	360	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2120 Å2
ΔGint	-6 kcal/mol
Surface area	16640 Å2
Method	PQS

Unit cell

Length a, b, c (Å)	93.512, 109.754, 100.301
Angle α, β, γ (deg.)	90.00, 94.35, 90.00
Int Tables number	4
Space group name H-M	P1211

Components

#1: Protein

A B C D E F G H I J K L
FAD3210

Details:

Mass: 18551.766 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) SYNTHETIC CONSTRUCT (others) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): K-12 / Variant (production host): XL1-BLUE

#2: Chemical

A-1168 C-1169 E-1169 G-1169 J-1169 K-1167
ChemComp-6FE / 4-[[4-(dimethylamino)cyclohexa-2,5-dien-1-ylidene]-(4-methoxyphenyl)methyl]-N,N-dimethyl-aniline / MALACHITE GREEN DERIVATIVE MG-2P

Details:

Mass: 360.492 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C₂₄H₂₈N₂O

#3: Water

ChemComp-HOH / water

Details:

Mass: 18.015 Da / Num. of mol.: 339 / Source method: isolated from a natural source / Formula: H₂O

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.25 ų/Da / Density % sol: 45.4 % / Description: NONE
• Crystal grow: Details: 20% (W/V) PEG3350, 0.2 M SODIUM MALONATE, PH 5.0

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1
• Detector: Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Oct 25, 2013 / Details: TOROIDAL FOCUSING
• Radiation: Monochromator: BARTELS MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 1 Å / Relative weight: 1
• Reflection: Resolution: 2.6→50 Å / Num. obs: 62041 / % possible obs: 100 % / Observed criterion σ(I): 0.86 / Redundancy: 6.9 % / B_iso Wilson estimate: 37.64 Ų / R_merge(I) obs: 0.2733 / Net I/σ(I): 6.6
• Reflection shell: Resolution: 2.6→2.693 Å / Redundancy: 2.36 % / Mean I/σ(I) obs: 0.86 / % possible all: 100

Processing

Software

Name	Version	Classification
PHENIX	(PHENIX.REFINE)	refinement
XDS		data reduction
XSCALE		data scaling
PHASER		phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1SVX

1svx

Resolution: 2.6→19.951 Å / SU ML: 0.47 / σ(F): 0.39 / Phase error: 30.83 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.2622	6090	5 %
Rwork	0.2135	-	-
obs	0.2159	61973	99.76 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso mean: 51.9 Ų

Refinement step

Cycle: LAST / Resolution: 2.6→19.951 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	14359	0	162	339	14860

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.001	14821
X-RAY DIFFRACTION	f_angle_d	0.453	20144
X-RAY DIFFRACTION	f_dihedral_angle_d	9.426	8550
X-RAY DIFFRACTION	f_chiral_restr	0.032	2253
X-RAY DIFFRACTION	f_plane_restr	0.003	2626

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
2.6-2.6295	0.388	203	0.3703	3809	X-RAY DIFFRACTION	100
2.6295-2.6604	0.3743	204	0.3832	3849	X-RAY DIFFRACTION	99
2.6604-2.6927	0.4284	193	0.3868	3772	X-RAY DIFFRACTION	98
2.6927-2.7267	0.4027	202	0.3595	3888	X-RAY DIFFRACTION	100
2.7267-2.7625	0.4145	206	0.3467	3863	X-RAY DIFFRACTION	100
2.7625-2.8003	0.3801	201	0.332	3839	X-RAY DIFFRACTION	100
2.8003-2.8402	0.3637	205	0.327	3896	X-RAY DIFFRACTION	100
2.8402-2.8824	0.3691	201	0.3059	3818	X-RAY DIFFRACTION	100
2.8824-2.9273	0.341	209	0.3038	3928	X-RAY DIFFRACTION	100
2.9273-2.9752	0.3492	206	0.2842	3861	X-RAY DIFFRACTION	100
2.9752-3.0263	0.3423	203	0.2822	3831	X-RAY DIFFRACTION	100
3.0263-3.0811	0.316	202	0.2782	3839	X-RAY DIFFRACTION	100
3.0811-3.1402	0.3244	211	0.2661	3938	X-RAY DIFFRACTION	100
3.1402-3.204	0.347	200	0.2683	3847	X-RAY DIFFRACTION	100
3.204-3.2734	0.3089	201	0.2515	3849	X-RAY DIFFRACTION	100
3.2734-3.3492	0.2892	200	0.2318	3893	X-RAY DIFFRACTION	100
3.3492-3.4325	0.2912	203	0.2403	3834	X-RAY DIFFRACTION	100
3.4325-3.5249	0.2886	204	0.2232	3798	X-RAY DIFFRACTION	99
3.5249-3.628	0.2292	204	0.1901	3882	X-RAY DIFFRACTION	100
3.628-3.7443	0.2363	205	0.181	3837	X-RAY DIFFRACTION	99
3.7443-3.8772	0.2111	203	0.1725	3864	X-RAY DIFFRACTION	100
3.8772-4.0313	0.2259	197	0.1683	3842	X-RAY DIFFRACTION	99
4.0313-4.2131	0.2071	207	0.1526	3858	X-RAY DIFFRACTION	100
4.2131-4.4329	0.1953	199	0.1381	3867	X-RAY DIFFRACTION	100
4.4329-4.7072	0.1839	201	0.1462	3859	X-RAY DIFFRACTION	100
4.7072-5.0652	0.2312	205	0.1607	3883	X-RAY DIFFRACTION	100
5.0652-5.5648	0.2646	207	0.1731	3884	X-RAY DIFFRACTION	100
5.5648-6.3473	0.2194	205	0.1827	3840	X-RAY DIFFRACTION	100
6.3473-7.9131	0.1792	201	0.1494	3867	X-RAY DIFFRACTION	100
7.9131-19.952	0.1595	202	0.1307	3812	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	9.3396	-2.0251	4.4659	6.8838	-0.9784	4.1902	0.1605	-1.4002	-0.4357	0.0921	-0.0525	0.2403	1.7174	-0.5071	-0.0621	0.5239	0.0323	0.1406	0.4745	0.0418	0.3123	-21.6977	9.4299	-18.8491
2	6.0335	0.4101	4.1893	6.1976	2.3533	4.0696	0.1321	-0.477	0.1019	0.8552	0.4006	0.4404	-0.4155	-0.4584	-0.4229	0.4956	0.0633	0.0669	0.4508	0.037	0.1987	-24.6671	18.7385	-16.4191
3	4.9339	2.1747	1.5627	1.6058	-0.4064	2.6409	-0.1239	0.4274	-0.7781	-0.3021	0.9085	-0.558	-0.0175	0.6363	0.2352	0.3282	0.3001	-0.0333	0.301	0.1391	0.2625	-16.1145	10.2849	-24.054
4	5.7871	0.1098	0.5274	3.1565	0.5754	6.2093	0.3112	-0.2002	0.2378	-0.5973	-0.0613	-0.0948	-0.926	-0.5973	-0.0067	0.4307	0.1709	0.0315	0.3577	0.0331	0.0991	-23.9189	18.4297	-27.4418
5	3.159	-0.8151	-0.4777	2.1955	0.541	4.8647	0.1564	-0.1318	-0.0822	0.073	-0.0744	0.1626	0.6593	-0.2131	-0.063	0.3437	-0.0165	0.0111	0.2923	0.0634	0.2333	-18.7718	14.3162	-35.0665
6	6.7161	2.5616	2.3148	2.358	2.0517	4.155	-0.4136	0.0551	0.8132	-0.7413	0.4888	-0.0465	-1.4946	-0.7456	-0.0139	0.4558	0.1363	0.0598	0.3198	0.0993	0.341	-23.2941	25.2114	-35.8407
7	2.6428	0.8021	0.1881	1.7246	1.798	3.4938	0.014	0.0789	-0.0491	0.0528	0.2608	-0.068	0.0499	-0.2573	-0.2936	0.2729	0.0105	0.0043	0.2537	0.0651	0.1962	-18.6997	14.5144	-44.4579
8	5.8305	4.4927	2.547	4.3205	3.2952	3.3074	0.0618	-0.1836	1.4247	-0.1502	-0.1439	0.8025	-0.7171	-1.2026	0.2729	0.2081	-0.0321	-0.0238	0.2857	0.0019	0.2875	-24.1415	24.7745	-46.5612
9	2.6079	0.7881	0.6782	2.1584	0.6058	4.1333	0.1032	0.6809	0.134	-0.2455	-0.0511	-0.2785	-0.0768	0.1386	-0.0587	0.3225	-0.0281	0.0441	0.3335	-0.0042	0.2225	-19.1531	14.4445	-53.9981
10	6.1809	5.755	-1.764	7.9379	0.0598	8.5887	0.5025	2.3202	1.0537	-0.3369	-0.4964	0.4729	-0.7919	-0.7633	-0.0234	0.4925	0.114	0.0591	0.6055	0.0464	0.3801	-26.7791	23.5806	-57.9825
11	7.238	-2.1139	4.6845	5.5887	1.5658	5.5651	0.4415	-0.2705	-1.5604	0.5791	-0.0995	0.4343	1.9065	-0.5565	-0.1851	0.7618	0.0331	0.0932	0.5978	0.0166	0.3812	-20.4271	32.4221	-69.3302
12	6.1342	1.5984	3.2254	5.2958	3.7194	7.2255	0.274	-1.2772	0.9702	1.4528	0.1426	0.4644	-1.287	-1.0277	0.3108	0.9673	0.0945	0.1027	0.4516	-0.0876	0.3377	-22.8335	41.4607	-66.7389
13	5.0081	-0.3542	0.1523	3.3101	-0.1903	6.5455	0.6464	-0.5852	0.1338	1.0911	-1.328	-0.31	1.2444	0.1258	0.2569	0.6439	-0.0093	-0.0666	0.4672	0.0619	0.4042	-14.0211	33.048	-73.9341
14	5.7249	-1.7037	-1.2089	3.2342	-0.1614	5.4543	0.4812	-0.3847	-0.4839	-0.264	-0.6402	0.5375	-0.0841	-0.9103	0.2976	0.2757	0.0579	0.008	0.4828	-0.0415	0.3811	-22.2693	37.0919	-79.8753
15	9.0978	-3.0652	1.9334	8.6943	-6.3889	4.7058	-0.2286	-0.8474	0.977	0.8311	-0.7288	0.1518	-1.3455	-0.7996	-1.5525	0.6869	0.2015	0.1654	0.5152	-0.2204	0.3298	-20.2132	46.3175	-75.3137
16	5.0988	0.8342	-0.4179	2.6575	-0.0338	6.9919	-0.1276	-0.2374	-0.0487	0.1334	-0.0574	0.3141	0.0128	-0.0281	0.1793	0.3083	0.0295	0.007	0.2316	0.0361	0.3533	-16.053	37.0729	-85.111
17	8.3277	2.9562	2.5668	2.0958	1.1029	6.6321	-0.1682	-0.4417	1.1915	0.331	-0.0574	0.3041	-1.2533	-0.4709	0.0485	0.503	0.101	0.0286	0.3493	-0.0248	0.3388	-20.1463	48.0845	-86.164
18	1.8706	0.257	-0.9501	2.4988	1.5079	5.438	-0.2614	-0.2514	0.0251	-0.3553	0.1778	-0.6377	-0.169	0.4958	-0.2707	0.2044	0.0052	-0.0245	0.2927	0.0377	0.3863	-11.6547	36.9706	-92.3217
19	2.1614	1.045	-0.3606	4.1926	0.9651	5.2904	-0.077	0.4266	0.3438	-0.0981	0.0358	-0.0164	-0.38	0.0949	0.0509	0.2002	-0.0004	0.008	0.2974	0.0436	0.3092	-17.3346	40.3358	-99.0658
20	4.3356	0.9292	0.7242	2.4285	0.7401	5.2718	-0.1103	0.638	0.1675	-1.0416	-0.2477	-0.0746	-0.911	-0.3195	0.2529	0.5084	0.1412	-0.0208	0.505	0.054	0.3481	-23.0592	40.1122	-107.6209
21	1.9978	-4.6058	-6.5761	3.4875	1.5451	5.7982	-0.2252	-1.4903	1.2842	0.7328	0.5303	-0.3894	-0.0974	0.6897	0.0036	0.5298	-0.0528	0.0265	0.7687	-0.0434	0.2281	-29.7625	24.4226	-70.1595
22	6.304	2.0212	-2.9471	5.0217	4.3022	7.6871	-0.2344	-1.4711	-0.9147	0.5589	-0.2879	-0.4041	2.3876	1.1768	0.4247	0.6508	0.2546	0.1049	0.9529	0.1908	0.3752	-28.1782	15.3356	-67.3301
23	2.3588	-2.1513	2.6197	3.8762	0.6002	7.6021	0.161	-0.3759	0.5119	-0.7632	0.3105	0.5709	-0.1454	-1.2688	-0.2915	0.3883	-0.0309	0.0383	0.4941	-0.0574	0.2449	-35.1209	24.2268	-75.9253
24	9.1037	-3.2258	-1.5072	3.2049	0.5686	6.9877	0.8954	-0.6429	0.166	-0.5922	-0.4204	-0.5713	0.1273	1.5163	-0.4176	0.4605	0.0438	0.0311	0.5955	0.0153	0.2087	-25.9588	19.9708	-80.2101
25	5.261	-1.8285	-0.2006	4.7469	-1.8275	9.2618	-0.2207	-0.5479	-0.4494	-0.1253	0.1932	0.6273	0.6991	0.203	0.0464	0.4092	-0.0507	0.0138	0.3473	0.0564	0.2989	-33.0236	17.1005	-81.5034
26	4.0413	-2.5236	-1.7676	3.5144	0.7912	6.1991	0.0017	-0.7838	0.335	-0.1387	0.2687	-0.6027	0.9135	1.0807	-0.1833	0.467	-0.0379	-0.0032	0.4593	-0.0025	0.2101	-24.9798	19.3256	-88.8413
27	8.0751	1.8135	-8.6878	3.1874	-0.6783	9.9674	-0.6304	-1.1423	-1.0336	-0.9525	-0.0468	-0.2127	2.3283	1.1866	0.1578	0.7007	0.1106	0.0477	0.2579	0.0148	0.2638	-26.9149	9.3188	-87.2727
28	2.6779	-0.3037	-0.5809	0.1939	-0.8522	5.1842	0.0125	0.1871	-0.1701	-0.1023	-0.2704	0.1714	0.6456	-0.4509	0.099	0.4014	-0.1639	-0.0159	0.3795	-0.0353	0.3546	-34.0675	20.9186	-94.5402
29	3.9724	1.7781	-1.9926	6.88	0.3313	5.2229	-0.2329	-0.1008	-0.4857	0.3692	-0.0016	-0.6861	0.5016	0.0994	0.3476	0.4908	0.0562	0.0222	0.3006	-0.0348	0.2148	-23.6976	15.4457	-97.5907
30	3.9074	-0.4829	-1.1338	4.6812	-2.8955	3.1642	0.2005	0.7355	-0.2799	-0.3331	-0.3158	0.2023	0.515	-0.8075	-0.0431	0.4159	-0.0752	0.0175	0.4846	-0.0883	0.1842	-31.9689	20.2984	-103.7914
31	6.9279	-1.3707	-0.3688	4.533	-2.2193	4.8431	0.2795	0.8067	-0.7834	-0.2567	-0.5363	0.3103	0.9271	0.7498	0.2378	0.4312	0.0708	0.0677	0.3905	-0.0439	0.2697	-20.5359	16.3672	-107.5725
32	1.9922	3.3942	0.8862	6.5567	0.0686	6.2247	-0.006	0.9263	-2.5173	0.3596	-0.7863	0.1519	1.0872	-0.3956	0.3548	0.8078	0.3081	0.1108	0.72	-0.1421	0.5827	-60.2401	-6.9351	-71.5075
33	5.4816	3.4409	4.007	7.0885	3.0757	2.9996	0.2237	1.5081	0.0396	-1.0758	0.1765	-0.8208	1.1139	0.5705	-0.6114	1.0554	0.0104	0.3367	1.0066	-0.2389	0.6153	-56.8922	-2.5073	-79.596
34	5.4367	-1.3606	-4.0257	8.4277	3.9989	5.8367	0.1606	0.6826	-0.8576	-1.1938	-0.2338	-0.1953	-0.1305	-0.2001	0.2843	0.4301	0.0643	0.0393	0.5011	-0.0894	0.6388	-65.9393	-2.4231	-68.6558
35	7.5798	-0.9598	2.4131	6.1018	3.4107	5.6948	0.5899	0.5047	0.9744	-0.4827	-0.2296	-0.9735	0.1745	0.2512	-0.3836	0.5777	0.072	0.093	0.4468	0.0489	0.3043	-56.6001	3.1371	-66.9338
36	1.9946	4.1111	3.3058	9.6633	4.0568	4.0032	-0.2322	1.366	0.9681	-1.3635	0.0572	-0.264	-0.3023	0.2228	-0.2761	0.4699	0.0321	0.2988	0.7262	0.0093	0.4202	-57.8586	7.233	-76.4875
37	4.1901	0.92	-3.2733	6.2652	1.9409	5.8787	0.2507	0.4974	-0.6297	0.0074	-0.1803	-0.4402	-0.7891	-0.7449	-0.0381	0.2702	0.0076	0.0887	0.6244	0.0878	0.4742	-66.7967	6.0941	-64.6713
38	1.6033	1.8902	1.2723	5.1444	0.6001	8.7376	0.5909	1.0914	0.6317	-0.1786	-0.1029	-1.238	-1.5713	1.1525	-0.0556	0.3837	0.0744	0.1941	0.6054	0.1418	0.5066	-56.3842	12.58	-65.2405
39	3.0005	-0.248	1.8436	2.3281	1.3909	5.8573	0.0085	0.405	0.6665	-0.3052	-0.0377	-0.1139	-0.8454	-0.2368	0.088	0.3875	0.0592	0.0302	0.2787	0.0989	0.4636	-61.142	17.5064	-55.9203
40	2.813	-1.4739	-0.0972	3.6899	-1.5846	9.1779	-0.3026	-0.5055	0.2497	0.3361	-0.4761	-0.5668	-0.8269	1.0506	0.5755	0.4345	-0.0046	-0.072	0.3682	0.0908	0.731	-52.6643	23.8085	-48.5443
41	8.0177	2.5128	-1.3624	4.6761	0.7693	0.6082	-0.7039	2.1152	-0.2496	-1.1555	0.261	0.6449	0.5948	-1.2938	0.2067	0.5658	-0.1865	0.088	0.5303	-0.4369	0.5402	-54.0983	-18.1751	-61.9563
42	3.4168	1.0018	0.3431	3.2592	2.8919	4.8084	-0.0699	0.4798	-0.319	0.0642	-0.2376	0.2717	0.1265	-0.474	0.1844	0.2951	-0.0655	0.0365	0.3337	-0.0785	0.418	-51.25	-10.0588	-52.6792
43	3.6045	-0.5897	-0.3413	5.0485	2.135	4.0609	-0.0261	-0.4418	0.1204	0.424	-0.1684	0.2144	0.1196	-0.0831	0.1425	0.2368	-0.0078	0.0425	0.31	-0.017	0.2597	-53.8823	1.4557	-37.8421
44	8.0863	3.319	3.5718	4.1161	-2.6802	8.0298	-0.0383	0.018	-1.3619	-1.062	-0.0766	-0.967	1.1575	0.2055	0.7278	0.3072	0.0694	0.1328	0.6127	-0.2784	0.635	-57.7145	14.614	-120.5244
45	5.4338	5.2337	6.4662	9.3076	5.489	7.828	-1.1529	1.7864	-0.5572	-1.6481	1.1879	-2.1685	0.1236	1.4773	-1.7799	0.6861	-0.2727	0.1201	0.9114	-0.3891	0.6074	-54.4195	18.2359	-128.9227
46	3.3299	1.4997	-0.8352	4.3903	2.5774	6.9098	0.0637	0.3859	-0.1958	0.0163	0.2962	-0.382	-0.5864	-0.2747	-0.1592	0.2367	-0.0108	0.0282	0.3759	-0.1149	0.4713	-59.3461	21.9608	-117.5351
47	6.3067	2.5984	6.1653	7.7851	4.8109	6.79	-0.4541	1.888	1.0172	-1.1411	0.3606	-0.5622	-2.2963	0.6277	-0.2093	0.5487	-0.0884	0.1185	0.7706	-0.1551	0.4647	-55.2627	28.3394	-126.6693
48	6.0387	2.2032	0.5358	5.431	1.3472	4.2485	0.1282	0.4418	-0.2213	0.2756	-0.0407	-0.3305	-0.4625	-0.2683	0.0027	0.4001	0.0861	-0.0342	0.3647	-0.094	0.4414	-59.6731	29.9195	-113.459
49	5.4661	0.7022	-0.766	4.3134	2.5171	8.0484	0.5033	0.2987	1.2995	-0.3796	-0.1193	0.2687	-1.3894	-0.1784	-0.1017	0.477	0.0553	-0.1295	0.3337	-0.011	0.5237	-59.419	36.4718	-113.3663
50	4.992	0.0662	1.7769	4.8033	0.6459	7.8038	-0.0058	-0.0716	0.6889	0.1985	-0.1103	-0.837	-0.9793	0.406	0.0488	0.4731	-0.0763	-0.0582	0.2454	-0.1231	0.5012	-52.3913	41.1479	-108.7248
51	2.2147	-0.3389	-0.0021	0.0836	0.1983	3.2574	-0.1205	-0.1578	1.518	-0.0576	-0.4194	0.1739	-1.569	-0.5386	-0.3977	0.6573	0.2086	-0.0962	0.2946	-0.3011	0.8281	-61.8243	43.1799	-102.6138
52	2.6126	-0.0343	-3.1329	5.2855	-4.2705	7.2332	-0.0961	-0.5061	1.1873	0.0697	-0.6118	-0.4842	-1.7379	0.9263	-0.0174	0.6462	-0.0658	-0.2452	0.2804	-0.1043	0.8749	-49.7718	46.6706	-99.9753
53	6.2685	-2.3448	-7.0657	3.7671	2.2107	8.0731	0.4784	1.888	-0.6811	-1.303	-0.1461	0.6466	-0.9382	-1.2531	0.4085	0.6191	-0.0574	-0.125	0.5358	-0.2103	0.5527	-49.5923	8.2641	-113.2265
54	1.7706	-0.5067	-0.669	5.3413	1.4162	1.3591	-0.0857	0.4122	-0.4523	0.2269	-0.5824	0.3819	0.3677	-0.3762	0.2038	0.4814	-0.0906	0.0763	0.2503	-0.4718	0.9292	-52.7504	0.501	-108.5345
55	3.3177	0.2778	-0.9569	6.9062	2.7533	4.5986	0.853	0.1138	-0.2163	0.3229	-0.6852	-1.0133	0.2756	-0.4719	-0.3592	0.382	-0.05	-0.0424	0.3844	-0.2084	0.6416	-43.8914	11.2922	-108.7053
56	3.701	0.0672	-0.9	3.6304	1.5261	3.7263	0.5926	-0.492	-0.7587	-0.0027	-0.6818	0.7817	0.396	-0.8299	-0.0663	0.4551	-0.1521	-0.027	0.3286	-0.1262	0.6001	-52.7544	8.9036	-101.2385
57	5.9153	0.3366	0.0198	6.8271	0.4299	6.745	0.2162	0.5246	-0.2663	0.6676	-0.2025	0.2621	-0.0131	-0.081	-0.1364	0.3431	-0.0576	-0.0198	0.3425	0.0079	0.3062	-43.0321	15.4733	-100.3168
58	3.2958	1.5005	-1.4054	3.5023	3.3708	7.76	-0.5343	-0.3447	0.0934	0.4428	-0.0711	0.8869	-0.1946	-1.1325	0.6766	0.4551	-0.082	-0.0153	0.3751	0.0216	0.4103	-53.5096	19.2679	-96.9927
59	9.1707	-3.2936	0.506	3.3113	0.5015	5.0199	0.4865	-1.6383	-0.9255	1.5202	-0.0989	1.5788	0.6338	-1.0271	-0.5824	0.586	-0.3081	0.0837	0.6452	0.0247	0.5726	-53.6621	11.1244	-90.6078
60	3.2301	-0.3921	-0.2	6.7335	1.9797	3.6689	0.3866	-0.6	0.1212	0.1117	-0.461	0.0273	0.2333	0.0225	0.06	0.3507	-0.0486	-0.0648	0.3537	-0.0586	0.3041	-43.8167	22.6483	-93.5349
61	2.6192	-3.766	2.3975	9.1538	0.1049	5.7044	0.3894	-0.8475	-0.0688	-0.4422	-0.602	1.2232	-0.5897	-1.1858	0.3118	0.3713	-0.0689	0.0793	0.6	-0.2281	0.4102	-54.0475	26.5546	-91.858
62	2.9535	0.802	-0.1302	4.7921	-3.8998	3.2827	0.0347	-1.2201	0.2479	1.578	0.4086	1.3718	0.6581	-0.3777	-0.4206	0.6175	-0.099	-0.0307	0.7575	-0.0348	0.3119	-55.406	19.639	-84.548
63	3.1107	1.3452	-0.261	2.2365	-1.5325	4.2305	0.1974	-0.4787	0.2007	-0.3354	-0.2669	-0.4002	0.2737	0.6109	-0.1793	0.3986	0.0177	-0.0478	0.398	-0.1464	0.4254	-45.3024	29.3638	-86.9643
64	8.4428	-2.9035	1.32	7.7516	-0.9396	7.4885	0.2393	-1.6239	0.8189	0.5768	-0.7351	0.2833	-0.1443	-0.967	0.6572	0.4776	-0.0364	0.019	0.5996	-0.2712	0.4838	-55.5586	34.8902	-87.0172
65	0.3531	0.4055	-0.5106	1.424	-0.5088	3.9079	-0.3345	-1.0492	-0.2124	0.6832	0.3966	0.8599	-0.3545	-0.3334	0.2484	-0.2354	0.2948	0.89	1.1844	-0.761	0.1896	-58.7657	29.2777	-78.6259
66	2.005	4.6065	1.2124	2.7769	0.3994	6.3787	0.0844	-0.3014	1.9496	0.2543	-0.3948	1.0517	-1.0212	-0.4072	0.1074	0.8803	0.2919	0.0428	0.6523	0.2074	0.8037	12.6413	36.2541	-57.93
67	0.0943	0.5624	0.3852	3.4859	2.3911	1.6299	-0.9809	0.479	0.8077	-1.4471	-0.2616	1.0638	-1.076	0.324	-1.3249	1.2406	0.1882	-0.0736	0.7897	0.7906	1.5027	10.925	38.0071	-67.309
68	5.0819	-1.9895	1.4487	2.2422	-1.6749	5.2795	-0.163	0.8073	0.7226	-0.1999	-0.7851	-0.8361	-0.1633	0.6714	0.699	0.4109	0.0559	0.029	0.6035	0.1577	0.7239	18.1718	30.6665	-57.6919
69	4.2268	1.5626	-0.6105	7.016	0.0789	4.6882	-0.0381	0.7016	1.0989	-0.3404	-0.6293	0.1389	-0.2734	-0.343	0.3943	0.4923	0.1397	-0.0267	0.6595	0.1665	0.5445	13.9128	24.6581	-63.2549
70	3.1814	1.2354	-2.8395	2.8874	1.5266	8.2637	0.11	0.8959	0.6023	0.1149	-0.6469	0.4546	0.1904	-0.8842	0.0706	0.4137	0.1599	-0.0281	0.6949	0.1069	0.4861	8.3932	16.9946	-61.0052
71	4.4319	2.6454	-1.6121	8.5915	0.2455	0.7872	0.7142	1.2049	0.3104	-2.0466	0.2273	0.564	0.3919	-0.1384	-0.2895	0.6774	0.324	-0.0608	0.8292	0.1039	0.4433	9.9775	17.5002	-71.1563
72	5.0193	0.7386	-0.2785	4.774	-1.1151	2.0325	0.2219	0.6638	0.0925	0.1839	-0.2416	0.149	-0.2195	-0.04	0.0188	0.4023	0.0394	0.031	0.5379	-0.0403	0.3986	13.1495	10.272	-59.0905
73	2.6112	2.0607	-0.5579	9.4963	-5.3469	3.17	-0.4817	1.6684	0.0158	-1.6799	0.0461	0.6292	1.0307	-0.705	-0.1819	0.2437	-0.0889	-0.0913	0.8949	-0.2799	0.4599	7.4812	7.4094	-69.2765
74	3.344	1.3661	-0.9087	5.3812	-2.1001	4.4957	-0.2526	0.5782	-0.7245	-0.8246	0.2207	-0.0086	0.5382	-0.3275	0.0544	0.4556	0.0397	0.0575	0.5694	-0.1385	0.5361	8.7391	0.0552	-59.7499
75	7.9395	-4.9482	-5.3988	5.3814	1.5958	5.0473	0.3058	-1.5866	1.0572	0.754	0.6329	-0.8854	0.0238	-0.1839	0.0793	1.0261	-0.0796	-0.227	0.7627	0.1017	0.4678	-32.684	0.128	-20.3108
76	0.4028	0.3367	-0.8979	0.3768	-0.4879	2.6598	0.0262	-0.5816	-0.5137	0.6215	0.0827	-0.2505	0.6429	-0.0814	0.7734	1.3067	-0.1579	-0.474	0.9514	0.9165	0.2351	-30.4443	-9.5059	-18.418
77	6.0806	-3.3428	1.676	3.4888	2.3723	7.0649	0.5324	-1.0386	0.2355	1.0445	0.1261	1.0839	-0.2259	-0.6224	-0.3628	0.5686	-0.0282	0.0397	0.4708	-0.0046	0.3617	-37.5327	-0.0515	-26.3336
78	2.4712	-1.0661	-2.2563	3.5846	-0.3544	2.6637	0.0262	-1.0457	-0.5708	0.5793	0.1363	-0.6086	0.7864	0.0433	0.3056	0.6227	-0.0073	-0.1647	0.4626	0.2986	0.5165	-29.5425	-8.329	-29.4673
79	7.3407	-2.5847	0.9024	3.6234	0.2213	2.2148	0.4738	-0.7978	-0.3591	0.4626	-0.6075	0.1404	0.2418	0.9384	-0.125	0.4208	-0.0842	0.0072	0.4296	0.0366	0.4152	-38.0112	-2.6335	-35.3836
80	6.9421	-1.2674	-0.6459	2.8489	-1.1644	6.8921	0.1986	0.2633	-0.5884	0.6281	-0.0144	-1.0809	-0.9843	0.6801	0.1421	0.3232	-0.0876	-0.0144	0.2165	-0.061	0.54	-27.328	-3.9832	-39.5557
81	2.6437	0.5654	-5.1313	2.4827	-0.2804	2.0051	-0.3674	-0.3145	-1.2619	-0.5801	0.0614	-0.6796	1.4236	0.8297	0.3889	0.5764	0.0834	-0.1594	0.2148	-0.0201	0.7583	-28.975	-14.0946	-38.5601
82	3.9472	1.0857	-0.4178	2.1394	-1.0048	5.3701	0.1944	0.2708	-0.2614	-0.3744	0.0174	0.109	0.4554	-0.007	-0.1241	0.3211	0.0054	0.004	0.28	-0.043	0.3726	-32.0248	-2.4451	-46.5092
83	5.8843	-1.7904	-1.7483	1.5632	-0.7831	5.3876	-0.1846	-0.0646	-0.9576	-1.0478	0.575	-1.1032	0.4325	0.7631	-0.0127	0.4289	0.0677	0.2859	0.1305	-0.1995	0.7179	-26.1381	-12.7051	-48.7788
84	1.7526	2.2826	-0.8976	2.8253	-1.4468	2.9849	-0.0708	0.4159	-0.3786	-0.2597	0.0086	-0.2175	0.5091	-0.265	0.0924	0.3997	-0.0173	0.0027	0.3851	-0.1116	0.3767	-29.7919	-1.6811	-55.8062
85	2.7198	1.4211	2.8135	6.9347	2.0769	7.2367	0.7236	0.9946	-2.3607	-0.2611	-0.1041	-1.4333	0.614	0.8394	-0.0798	0.6431	0.0387	0.18	0.4916	-0.1689	0.7612	-21.8898	-10.6648	-59.3607
86	4.0997	0.9786	-0.8952	6.8232	2.9796	4.9389	-0.5918	0.9312	0.1556	-0.1152	-0.3173	0.3197	-2.2794	0.5117	0.5322	1.0305	-0.2983	-0.1474	0.6593	0.1932	0.6429	2.398	38.0665	-49.1985
87	4.0478	3.8779	0.1073	4.6013	0.1647	0.2788	-0.2028	-0.2954	1.028	0.6448	-0.4411	-0.1999	-0.7892	0.2214	-1.741	1.4242	-0.8657	-0.0565	0.4748	0.1011	0.8262	5.2277	41.2545	-39.8987
88	4.2762	1.9725	0.9403	6.759	-1.6446	0.91	0.0638	0.1988	0.5184	0.3625	-0.6441	0.541	-1.3252	0.637	0.3417	0.7348	-0.1258	-0.0662	0.4406	0.1182	0.565	-3.1847	32.9803	-47.982
89	6.9221	-0.5966	2.0768	8.621	1.7822	7.9545	-0.2055	0.1992	0.5871	0.8416	0.1612	-1.2883	-0.9851	1.8461	-0.2441	0.5971	-0.2228	-0.043	0.4406	0.0204	0.608	4.4765	30.1351	-39.32
90	3.8409	-0.2169	1.3645	5.8688	-1.2157	4.4903	0.0383	0.6362	1.0765	-0.4941	0.2215	-0.9159	-0.3203	0.8732	-0.2653	0.2341	-0.0792	0.0442	0.4141	0.0603	0.3991	-0.5411	22.3011	-43.1464
91	6.812	-1.9941	-1.3065	6.3434	1.254	0.3988	0.3468	-1.5029	1.0224	1.783	0.3063	-1.0741	0.3812	1.5084	-0.1249	0.5006	-0.1026	-0.1497	0.8535	0.053	0.4817	3.9049	22.1031	-32.1308
92	2.1441	-0.4144	0.3216	3.6125	-0.4842	2.0643	0.0266	-0.0685	-0.1539	-0.1172	0.0651	-0.3039	0.082	0.5796	-0.0374	0.3655	0.0211	0.0385	0.3821	0.0088	0.2755	-0.4693	12.9214	-42.3887
93	7.0507	-0.108	-1.7625	1.9322	1.1952	2.0202	-0.4335	-1.2676	0.0701	1.6547	0.5383	-1.5213	0.215	1.1536	-0.2222	0.4453	0.0306	-0.1276	0.5988	0.0686	0.4824	4.9774	11.5608	-31.7855
94	1.9368	-1.0485	0.0914	4.6874	2.6922	9.6398	-0.2817	-0.083	-0.303	0.5547	-0.3518	0.4492	1.1338	-0.1545	0.715	0.2607	0.0314	0.0379	0.3442	-0.0053	0.344	-4.169	5.6315	-41.3692
95	0.249	-1.0742	0.029	4.5794	-0.9942	6.0963	-1.092	-0.9727	0.2016	0.2728	0.0816	-0.3393	0.4041	0.513	0.8082	0.2318	0.1435	-0.0287	0.3751	-0.0185	0.5306	6.1027	0.4404	-42.5714
96	1.9673	0.4744	-0.0549	2.4176	-0.4561	9.6618	-0.2738	-1.2485	-0.6592	0.937	0.08	-1.1477	0.8292	1.6556	0.0278	0.6943	0.1632	-0.0558	0.7501	0.0273	0.5171	7.4839	0.1816	-32.2516
97	4.5863	0.2717	-0.2385	8.6513	0.4748	5.5971	-0.3453	1.072	0.7386	-1.0943	0.3795	-0.8379	-1.5109	0.6937	-0.1949	1.2111	-0.0983	-0.0558	0.7843	-0.3253	1.154	0.2073	3.9616	-91.5524
98	4.2617	-2.0679	-0.0544	4.6445	0.4454	2.4977	-0.5239	-0.632	-0.1067	0.8207	-0.1174	0.279	-2.0254	0.0381	0.3461	1.176	-0.0657	-0.1269	0.8029	-0.3969	1.5556	2.0426	5.5687	-82.0532
99	6.36	2.464	2.09	1.7125	0.8994	4.7343	-0.013	-0.275	1.5609	0.2192	-0.6543	0.4475	-0.2855	-1.3405	0.7362	0.7916	0.0627	0.0275	0.6963	-0.0868	0.9902	-5.4847	-1.5283	-91.7276
100	6.0028	-2.7729	-2.252	6.3017	1.498	5.7948	-0.148	-0.8822	1.0781	1.5097	0.2754	-0.7244	0.6194	0.3875	-0.0215	0.7294	-0.2445	-0.0784	0.7892	-0.2045	0.7674	2.4057	-5.4407	-83.8271
101	7.7923	-2.0817	0.712	6.7782	0.0653	3.3304	0.1127	-0.6699	1.7873	-0.554	-0.0612	-0.1346	-0.3865	0.276	-0.2139	0.4456	-0.1584	0.0023	0.6549	0.0577	0.5252	-1.7732	-12.7512	-89.3409
102	4.1112	-1.4783	-1.9826	5.3466	0.8234	0.9921	-0.1113	-1.0888	0.1879	0.7722	-0.0071	-0.0671	0.0472	0.4484	-0.0399	0.413	-0.0742	-0.0087	0.7496	0.1447	0.3588	0.3449	-20.0599	-87.329
103	3.7515	0.0101	-0.2701	5.2967	2.5363	6.0624	-0.3314	-0.9374	-0.8811	0.3932	0.374	0.2665	0.3349	0.4138	-0.0346	0.4215	-0.0398	0.0284	0.7445	0.2278	0.5079	0.1565	-27.9741	-87.0101
104	4.1998	-1.8152	-0.7297	5.0466	3.6059	8.2066	-0.3649	-0.8242	-0.6555	1.0134	0.6148	-0.6292	1.6689	0.2969	0.239	0.5335	-0.0711	0.0132	0.6131	0.2184	0.7574	8.7619	-34.148	-89.5047
105	1.7575	-0.5914	0.4401	2.4367	-0.6861	0.6762	-0.0942	-0.5247	-0.1882	0.5092	0.1569	0.8637	-0.6574	-0.4981	0.1432	0.8685	0.5324	0.0603	0.664	-0.0663	0.7143	9.2061	5.5147	-101.0099
106	1.3125	2.5356	1.4698	8.7801	0.6489	5.6542	0.1195	0.2347	0.8327	-1.162	0.0722	0.7104	-2.0783	-1.1022	0.2983	1.0829	0.477	-0.1437	0.7672	0.1398	1.134	7.031	8.8885	-109.966
107	3.1965	0.0776	1.4281	1.7722	-1.4016	4.6871	0.2711	-0.4202	0.6615	0.7004	-0.7938	-0.2373	-1.2041	0.0844	0.5001	0.7555	0.081	0.0538	0.4905	-0.0547	0.7854	15.5397	0.6875	-101.9252
108	6.4339	-0.0765	1.2952	7.0685	0.5253	4.465	-0.0724	0.1145	0.3945	-1.0501	-0.3612	0.7846	-0.5016	-1.6523	0.1153	0.4933	0.1963	-0.043	0.6125	-0.0523	0.5163	7.7768	-2.3089	-110.5613
109	2.6358	0.0286	1.7722	7.3355	0.4402	4.1153	0.241	-0.9893	1.0955	0.1816	-0.1618	0.65	-0.4151	-1.0492	-0.2855	0.4304	0.0563	0.0483	0.3972	-0.0114	0.5113	12.8368	-10.0553	-106.8335
110	3.6321	2.0286	-1.3591	3.9494	-0.8148	1.4363	0.6058	0.7361	-0.1569	-1.2876	-0.5647	1.5186	-0.1655	-1.1082	-0.3055	0.4311	0.1447	-0.1549	0.7132	0.0057	0.5047	8.3815	-10.2252	-117.8589
111	2.0197	0.3665	0.1419	2.9417	0.0544	2.534	-0.1171	0.0127	-0.0306	-0.2358	0.0202	0.4502	0.2943	-0.8099	0.1168	0.3279	-0.092	-0.0245	0.4544	0.0185	0.4357	12.8265	-19.4616	-107.6165
112	4.9052	-0.8026	2.0666	7.6249	-2.4689	1.4726	-0.1882	2.1126	0.0821	-0.9769	-0.1755	0.3987	0.9676	-0.5552	-0.0017	-0.0374	-0.4512	0.0645	0.7303	-0.0848	0.5827	7.3934	-20.7375	-118.2476
113	3.5342	0.3968	0.527	4.8453	0.2066	3.9527	0.3886	0.5807	-0.4591	-0.2022	0.0421	0.4238	0.704	-0.6939	-0.229	0.3266	-0.037	-0.0607	0.3394	-0.0034	0.3868	16.572	-26.7347	-108.7113
114	6.1214	-2.6431	-5.07	5.2287	-0.1212	5.5315	-0.114	0.5768	-1.4874	-1.3206	-0.0131	0.5629	0.4791	-1.0369	0.683	0.2774	-0.2613	0.0086	0.6888	0.1344	0.7541	6.295	-31.9239	-107.5131
115	2.4017	0.1087	-2.0012	7.8305	-2.5479	5.2395	0.2551	0.809	-1.319	-0.9692	-0.0736	-0.0194	1.7571	-0.3664	-0.0421	0.8375	-0.2802	-0.3155	0.8651	-0.1496	0.8344	4.651	-32.1055	-117.3792

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	CHAIN 'A' AND (RESID 12 THROUGH 24 )
2	X-RAY DIFFRACTION	2	CHAIN 'A' AND (RESID 25 THROUGH 35 )
3	X-RAY DIFFRACTION	3	CHAIN 'A' AND (RESID 36 THROUGH 49 )
4	X-RAY DIFFRACTION	4	CHAIN 'A' AND (RESID 50 THROUGH 68 )
5	X-RAY DIFFRACTION	5	CHAIN 'A' AND (RESID 69 THROUGH 92 )
6	X-RAY DIFFRACTION	6	CHAIN 'A' AND (RESID 93 THROUGH 101 )
7	X-RAY DIFFRACTION	7	CHAIN 'A' AND (RESID 102 THROUGH 125 )
8	X-RAY DIFFRACTION	8	CHAIN 'A' AND (RESID 126 THROUGH 134 )
9	X-RAY DIFFRACTION	9	CHAIN 'A' AND (RESID 135 THROUGH 158 )
10	X-RAY DIFFRACTION	10	CHAIN 'A' AND (RESID 159 THROUGH 167 )
11	X-RAY DIFFRACTION	11	CHAIN 'B' AND (RESID 13 THROUGH 24 )
12	X-RAY DIFFRACTION	12	CHAIN 'B' AND (RESID 25 THROUGH 35 )
13	X-RAY DIFFRACTION	13	CHAIN 'B' AND (RESID 36 THROUGH 49 )
14	X-RAY DIFFRACTION	14	CHAIN 'B' AND (RESID 50 THROUGH 59 )
15	X-RAY DIFFRACTION	15	CHAIN 'B' AND (RESID 60 THROUGH 68 )
16	X-RAY DIFFRACTION	16	CHAIN 'B' AND (RESID 69 THROUGH 92 )
17	X-RAY DIFFRACTION	17	CHAIN 'B' AND (RESID 93 THROUGH 101 )
18	X-RAY DIFFRACTION	18	CHAIN 'B' AND (RESID 102 THROUGH 115 )
19	X-RAY DIFFRACTION	19	CHAIN 'B' AND (RESID 116 THROUGH 148 )
20	X-RAY DIFFRACTION	20	CHAIN 'B' AND (RESID 149 THROUGH 166 )
21	X-RAY DIFFRACTION	21	CHAIN 'C' AND (RESID 13 THROUGH 24 )
22	X-RAY DIFFRACTION	22	CHAIN 'C' AND (RESID 25 THROUGH 35 )
23	X-RAY DIFFRACTION	23	CHAIN 'C' AND (RESID 36 THROUGH 49 )
24	X-RAY DIFFRACTION	24	CHAIN 'C' AND (RESID 50 THROUGH 59 )
25	X-RAY DIFFRACTION	25	CHAIN 'C' AND (RESID 60 THROUGH 82 )
26	X-RAY DIFFRACTION	26	CHAIN 'C' AND (RESID 83 THROUGH 92 )
27	X-RAY DIFFRACTION	27	CHAIN 'C' AND (RESID 93 THROUGH 101 )
28	X-RAY DIFFRACTION	28	CHAIN 'C' AND (RESID 102 THROUGH 115 )
29	X-RAY DIFFRACTION	29	CHAIN 'C' AND (RESID 116 THROUGH 134 )
30	X-RAY DIFFRACTION	30	CHAIN 'C' AND (RESID 135 THROUGH 148 )
31	X-RAY DIFFRACTION	31	CHAIN 'C' AND (RESID 149 THROUGH 168 )
32	X-RAY DIFFRACTION	32	CHAIN 'D' AND (RESID 13 THROUGH 24 )
33	X-RAY DIFFRACTION	33	CHAIN 'D' AND (RESID 25 THROUGH 35 )
34	X-RAY DIFFRACTION	34	CHAIN 'D' AND (RESID 36 THROUGH 49 )
35	X-RAY DIFFRACTION	35	CHAIN 'D' AND (RESID 50 THROUGH 59 )
36	X-RAY DIFFRACTION	36	CHAIN 'D' AND (RESID 60 THROUGH 68 )
37	X-RAY DIFFRACTION	37	CHAIN 'D' AND (RESID 69 THROUGH 82 )
38	X-RAY DIFFRACTION	38	CHAIN 'D' AND (RESID 83 THROUGH 101 )
39	X-RAY DIFFRACTION	39	CHAIN 'D' AND (RESID 102 THROUGH 148 )
40	X-RAY DIFFRACTION	40	CHAIN 'D' AND (RESID 149 THROUGH 166 )
41	X-RAY DIFFRACTION	41	CHAIN 'E' AND (RESID 12 THROUGH 35 )
42	X-RAY DIFFRACTION	42	CHAIN 'E' AND (RESID 36 THROUGH 92 )
43	X-RAY DIFFRACTION	43	CHAIN 'E' AND (RESID 93 THROUGH 168 )
44	X-RAY DIFFRACTION	44	CHAIN 'F' AND (RESID 13 THROUGH 24 )
45	X-RAY DIFFRACTION	45	CHAIN 'F' AND (RESID 25 THROUGH 35 )
46	X-RAY DIFFRACTION	46	CHAIN 'F' AND (RESID 36 THROUGH 59 )
47	X-RAY DIFFRACTION	47	CHAIN 'F' AND (RESID 60 THROUGH 68 )
48	X-RAY DIFFRACTION	48	CHAIN 'F' AND (RESID 69 THROUGH 92 )
49	X-RAY DIFFRACTION	49	CHAIN 'F' AND (RESID 93 THROUGH 115 )
50	X-RAY DIFFRACTION	50	CHAIN 'F' AND (RESID 116 THROUGH 134 )
51	X-RAY DIFFRACTION	51	CHAIN 'F' AND (RESID 135 THROUGH 148 )
52	X-RAY DIFFRACTION	52	CHAIN 'F' AND (RESID 149 THROUGH 166 )
53	X-RAY DIFFRACTION	53	CHAIN 'G' AND (RESID 13 THROUGH 24 )
54	X-RAY DIFFRACTION	54	CHAIN 'G' AND (RESID 25 THROUGH 35 )
55	X-RAY DIFFRACTION	55	CHAIN 'G' AND (RESID 36 THROUGH 49 )
56	X-RAY DIFFRACTION	56	CHAIN 'G' AND (RESID 50 THROUGH 68 )
57	X-RAY DIFFRACTION	57	CHAIN 'G' AND (RESID 69 THROUGH 82 )
58	X-RAY DIFFRACTION	58	CHAIN 'G' AND (RESID 83 THROUGH 92 )
59	X-RAY DIFFRACTION	59	CHAIN 'G' AND (RESID 93 THROUGH 101 )
60	X-RAY DIFFRACTION	60	CHAIN 'G' AND (RESID 102 THROUGH 115 )
61	X-RAY DIFFRACTION	61	CHAIN 'G' AND (RESID 116 THROUGH 125 )
62	X-RAY DIFFRACTION	62	CHAIN 'G' AND (RESID 126 THROUGH 134 )
63	X-RAY DIFFRACTION	63	CHAIN 'G' AND (RESID 135 THROUGH 148 )
64	X-RAY DIFFRACTION	64	CHAIN 'G' AND (RESID 149 THROUGH 158 )
65	X-RAY DIFFRACTION	65	CHAIN 'G' AND (RESID 159 THROUGH 168 )
66	X-RAY DIFFRACTION	66	CHAIN 'H' AND (RESID 13 THROUGH 24 )
67	X-RAY DIFFRACTION	67	CHAIN 'H' AND (RESID 25 THROUGH 35 )
68	X-RAY DIFFRACTION	68	CHAIN 'H' AND (RESID 36 THROUGH 49 )
69	X-RAY DIFFRACTION	69	CHAIN 'H' AND (RESID 50 THROUGH 82 )
70	X-RAY DIFFRACTION	70	CHAIN 'H' AND (RESID 83 THROUGH 92 )
71	X-RAY DIFFRACTION	71	CHAIN 'H' AND (RESID 93 THROUGH 101 )
72	X-RAY DIFFRACTION	72	CHAIN 'H' AND (RESID 102 THROUGH 125 )
73	X-RAY DIFFRACTION	73	CHAIN 'H' AND (RESID 126 THROUGH 134 )
74	X-RAY DIFFRACTION	74	CHAIN 'H' AND (RESID 135 THROUGH 166 )
75	X-RAY DIFFRACTION	75	CHAIN 'I' AND (RESID 12 THROUGH 24 )
76	X-RAY DIFFRACTION	76	CHAIN 'I' AND (RESID 25 THROUGH 35 )
77	X-RAY DIFFRACTION	77	CHAIN 'I' AND (RESID 36 THROUGH 49 )
78	X-RAY DIFFRACTION	78	CHAIN 'I' AND (RESID 50 THROUGH 68 )
79	X-RAY DIFFRACTION	79	CHAIN 'I' AND (RESID 69 THROUGH 82 )
80	X-RAY DIFFRACTION	80	CHAIN 'I' AND (RESID 83 THROUGH 92 )
81	X-RAY DIFFRACTION	81	CHAIN 'I' AND (RESID 93 THROUGH 101 )
82	X-RAY DIFFRACTION	82	CHAIN 'I' AND (RESID 102 THROUGH 125 )
83	X-RAY DIFFRACTION	83	CHAIN 'I' AND (RESID 126 THROUGH 134 )
84	X-RAY DIFFRACTION	84	CHAIN 'I' AND (RESID 135 THROUGH 158 )
85	X-RAY DIFFRACTION	85	CHAIN 'I' AND (RESID 159 THROUGH 168 )
86	X-RAY DIFFRACTION	86	CHAIN 'J' AND (RESID 12 THROUGH 24 )
87	X-RAY DIFFRACTION	87	CHAIN 'J' AND (RESID 25 THROUGH 35 )
88	X-RAY DIFFRACTION	88	CHAIN 'J' AND (RESID 36 THROUGH 49 )
89	X-RAY DIFFRACTION	89	CHAIN 'J' AND (RESID 50 THROUGH 68 )
90	X-RAY DIFFRACTION	90	CHAIN 'J' AND (RESID 69 THROUGH 92 )
91	X-RAY DIFFRACTION	91	CHAIN 'J' AND (RESID 93 THROUGH 101 )
92	X-RAY DIFFRACTION	92	CHAIN 'J' AND (RESID 102 THROUGH 125 )
93	X-RAY DIFFRACTION	93	CHAIN 'J' AND (RESID 126 THROUGH 134 )
94	X-RAY DIFFRACTION	94	CHAIN 'J' AND (RESID 135 THROUGH 148 )
95	X-RAY DIFFRACTION	95	CHAIN 'J' AND (RESID 149 THROUGH 158 )
96	X-RAY DIFFRACTION	96	CHAIN 'J' AND (RESID 159 THROUGH 168 )
97	X-RAY DIFFRACTION	97	CHAIN 'K' AND (RESID 13 THROUGH 24 )
98	X-RAY DIFFRACTION	98	CHAIN 'K' AND (RESID 25 THROUGH 35 )
99	X-RAY DIFFRACTION	99	CHAIN 'K' AND (RESID 36 THROUGH 49 )
100	X-RAY DIFFRACTION	100	CHAIN 'K' AND (RESID 50 THROUGH 68 )
101	X-RAY DIFFRACTION	101	CHAIN 'K' AND (RESID 69 THROUGH 92 )
102	X-RAY DIFFRACTION	102	CHAIN 'K' AND (RESID 93 THROUGH 125 )
103	X-RAY DIFFRACTION	103	CHAIN 'K' AND (RESID 126 THROUGH 148 )
104	X-RAY DIFFRACTION	104	CHAIN 'K' AND (RESID 149 THROUGH 166 )
105	X-RAY DIFFRACTION	105	CHAIN 'L' AND (RESID 13 THROUGH 24 )
106	X-RAY DIFFRACTION	106	CHAIN 'L' AND (RESID 25 THROUGH 35 )
107	X-RAY DIFFRACTION	107	CHAIN 'L' AND (RESID 36 THROUGH 49 )
108	X-RAY DIFFRACTION	108	CHAIN 'L' AND (RESID 50 THROUGH 68 )
109	X-RAY DIFFRACTION	109	CHAIN 'L' AND (RESID 69 THROUGH 92 )
110	X-RAY DIFFRACTION	110	CHAIN 'L' AND (RESID 93 THROUGH 101 )
111	X-RAY DIFFRACTION	111	CHAIN 'L' AND (RESID 102 THROUGH 125 )
112	X-RAY DIFFRACTION	112	CHAIN 'L' AND (RESID 126 THROUGH 134 )
113	X-RAY DIFFRACTION	113	CHAIN 'L' AND (RESID 135 THROUGH 148 )
114	X-RAY DIFFRACTION	114	CHAIN 'L' AND (RESID 149 THROUGH 158 )
115	X-RAY DIFFRACTION	115	CHAIN 'L' AND (RESID 159 THROUGH 167 )