negative regulation of L-glutamate import across plasma membrane / negative regulation of bile acid secretion / response to Gram-negative bacterium / positive regulation of neutrophil activation / negative regulation of branching involved in lung morphogenesis / positive regulation of interleukin-33 production / positive regulation of blood microparticle formation / positive regulation of chronic inflammatory response to antigenic stimulus / positive regulation of fractalkine production / positive regulation of vitamin D biosynthetic process ...negative regulation of L-glutamate import across plasma membrane / negative regulation of bile acid secretion / response to Gram-negative bacterium / positive regulation of neutrophil activation / negative regulation of branching involved in lung morphogenesis / positive regulation of interleukin-33 production / positive regulation of blood microparticle formation / positive regulation of chronic inflammatory response to antigenic stimulus / positive regulation of fractalkine production / positive regulation of vitamin D biosynthetic process / response to macrophage colony-stimulating factor / positive regulation of leukocyte adhesion to arterial endothelial cell / positive regulation of translational initiation by iron / response to 3,3',5-triiodo-L-thyronine / regulation of membrane lipid metabolic process / regulation of endothelial cell apoptotic process / regulation of branching involved in salivary gland morphogenesis / chronic inflammatory response to antigenic stimulus / negative regulation of protein-containing complex disassembly / response to gold nanoparticle / positive regulation of humoral immune response mediated by circulating immunoglobulin / : / positive regulation of hair follicle development / negative regulation of myelination / death receptor agonist activity / negative regulation of vascular wound healing / negative regulation of bicellular tight junction assembly / negative regulation of amyloid-beta clearance / negative regulation of cytokine production involved in immune response / response to isolation stress / inflammatory response to wounding / cellular response to toxic substance / positive regulation of calcidiol 1-monooxygenase activity / positive regulation of I-kappaB phosphorylation / sequestering of triglyceride / positive regulation of interleukin-18 production / positive regulation of action potential / TNF signaling / positive regulation of protein transport / epithelial cell proliferation involved in salivary gland morphogenesis / toll-like receptor 3 signaling pathway / embryonic digestive tract development / leukocyte migration involved in inflammatory response / necroptotic signaling pathway / vascular endothelial growth factor production / positive regulation of calcineurin-NFAT signaling cascade / positive regulation of neuroinflammatory response / positive regulation of mononuclear cell migration / leukocyte tethering or rolling / response to fructose / positive regulation of synoviocyte proliferation / positive regulation of fever generation / negative regulation of myoblast differentiation / regulation of establishment of endothelial barrier / negative regulation of glucose import / endothelial cell apoptotic process / macrophage activation involved in immune response / positive regulation of protein localization to cell surface / negative regulation of oxidative phosphorylation / TNFR1-mediated ceramide production / positive regulation of cytokine production involved in inflammatory response / positive regulation of osteoclast differentiation / regulation of metabolic process / tumor necrosis factor receptor binding / negative regulation of systemic arterial blood pressure / positive regulation of extrinsic apoptotic signaling pathway / positive regulation of chemokine (C-X-C motif) ligand 2 production / positive regulation of programmed cell death / regulation of immunoglobulin production / positive regulation of hepatocyte proliferation / positive regulation of protein-containing complex disassembly / positive regulation of heterotypic cell-cell adhesion / positive regulation of podosome assembly / positive regulation of membrane protein ectodomain proteolysis / negative regulation of blood vessel endothelial cell migration / negative regulation of lipid storage / regulation of canonical NF-kappaB signal transduction / negative regulation of mitotic cell cycle / regulation of reactive oxygen species metabolic process / regulation of fat cell differentiation / TNFR1-induced proapoptotic signaling / positive regulation of leukocyte adhesion to vascular endothelial cell / cortical actin cytoskeleton organization / response to L-glutamate / negative regulation of heart rate / positive regulation of amyloid-beta formation / positive regulation of DNA biosynthetic process / negative regulation of viral genome replication / negative regulation of osteoblast differentiation / regulation of synapse organization / negative regulation of fat cell differentiation / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / negative regulation of endothelial cell proliferation / Interleukin-10 signaling / negative regulation of interleukin-6 production / regulation of insulin secretion / positive regulation of glial cell proliferation / phagocytic cup / humoral immune response / negative regulation of signaling receptor activity Similarity search - Function
Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Aug 17, 2015 Details: Si (111) monochromator followed by a toroidal focusing mirror
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1 Å / Relative weight: 1
Reflection
Resolution: 3→184.24 Å / Num. obs: 158847 / % possible obs: 90.6 % / Observed criterion σ(I): 0 / Redundancy: 1.9 % / Rmerge(I) obs: 0.068 / Net I/σ(I): 7.58
Reflection shell
Resolution: 3→3.25 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.677 / % possible all: 90.4
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Processing
Software
Name
Version
Classification
XDS
datareduction
XSCALE
datascaling
REFMAC
5.8.0049
refinement
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3→184.24 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.906 / SU B: 69.685 / SU ML: 0.573 / Cross valid method: THROUGHOUT / ESU R Free: 0.555 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.3
445
0.3 %
RANDOM
Rwork
0.21107
-
-
-
obs
0.21131
158401
90.6 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å