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- PDB-5a5d: A complex of the synthetic siderophore analogue Fe(III)-5-LICAM w... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5a5d | ||||||
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Title | A complex of the synthetic siderophore analogue Fe(III)-5-LICAM with the CeuE periplasmic protein from Campylobacter jejuni | ||||||
![]() | ENTEROCHELIN UPTAKE PERIPLASMIC BINDING PROTEIN | ||||||
![]() | TRANSPORT PROTEIN / SYNTHETIC SIDEROPHORE / LIGAND COMPLEX / PERIPLASMIC BINDING PROTEIN / TETRADENTATE SIDEROPHORES | ||||||
Function / homology | ![]() outer membrane-bounded periplasmic space / iron ion transport / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Blagova, E. / Hughes, A. / Moroz, O.V. / Raines, D.J. / Wilde, E.J. / Turkenburg, J.P. / Duhme-Klair, A.-K. / Wilson, K.S. | ||||||
![]() | ![]() Title: Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM(4-) siderophore analogues of varied linker length. Authors: Wilde, E.J. / Hughes, A. / Blagova, E.V. / Moroz, O.V. / Thomas, R.P. / Turkenburg, J.P. / Raines, D.J. / Duhme-Klair, A.K. / Wilson, K.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.1 KB | Display | ![]() |
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PDB format | ![]() | 56.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5a5vC ![]() 5ad1C ![]() 5lwhC ![]() 5lwqC ![]() 5mbqC ![]() 5mbtC ![]() 5mbuC ![]() 5tcyC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 31927.822 Da / Num. of mol.: 1 / Fragment: RESIDUES 24-310 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-FE / |
#3: Chemical | ChemComp-5LC / |
#4: Water | ChemComp-HOH / |
Sequence details | N-TERMINAL TRUNCATION, FIRST TWO RESIDUES ALA22 AND MET23 ARE REMAINING FROM THE PLASMID AFTER ...N-TERMINAL TRUNCATION |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47 % / Description: NONE |
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Crystal grow | Details: PACT H12, 0.2M SODIUM MALONATE, 0.1M BIS-TRIS-PROPANE PH 8.5, 20% PEG3350 |
-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.74→46.07 Å / Num. obs: 30349 / % possible obs: 98 % / Observed criterion σ(I): 2 / Redundancy: 6.5 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 19.7 |
Reflection shell | Resolution: 1.74→1.78 Å / Redundancy: 6.5 % / Rmerge(I) obs: 0.7 / Mean I/σ(I) obs: 2.7 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 1.74→46.07 Å / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Refinement step | Cycle: LAST / Resolution: 1.74→46.07 Å
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