+Open data
-Basic information
Entry | Database: PDB / ID: 4zxq | ||||||
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Title | P22 Tail Needle Gp26 1-140 crystallized at pH 3.9 | ||||||
Components | Tail needle protein gp26 | ||||||
Keywords | VIRAL PROTEIN / P22 / Tail Needle / Membrane penetration | ||||||
Function / homology | symbiont genome ejection through host cell envelope, short tail mechanism / virus tail / symbiont genome entry into host cell via pore formation in plasma membrane / Tail needle protein gp26 Function and homology information | ||||||
Biological species | Enterobacteria phage P22 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.75 Å | ||||||
Authors | Sankhala, R.S. / Cingolani, G. | ||||||
Funding support | United States, 1items
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Citation | Journal: J.Biol.Chem. / Year: 2016 Title: Structural Plasticity of the Protein Plug That Traps Newly Packaged Genomes in Podoviridae Virions. Authors: Bhardwaj, A. / Sankhala, R.S. / Olia, A.S. / Brooke, D. / Casjens, S.R. / Taylor, D.J. / Prevelige, P.E. / Cingolani, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4zxq.cif.gz | 177.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4zxq.ent.gz | 141.7 KB | Display | PDB format |
PDBx/mmJSON format | 4zxq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zx/4zxq ftp://data.pdbj.org/pub/pdb/validation_reports/zx/4zxq | HTTPS FTP |
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-Related structure data
Related structure data | 4zkpC 4zkuC 5bu5C 5bu8C 5bvzC 2pohS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 15306.830 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Details: Bacteriophage P22 / Source: (gene. exp.) Enterobacteria phage P22 (virus) / Gene: 26 / Cell line (production host): BL21(DE3) / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P35837 #2: Chemical | #3: Chemical | ChemComp-CL / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.95 Å3/Da / Density % sol: 58.28 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 3.9 Details: 20% PEG 8000, 0.05 M Potassium phosphate monobasic pH 3.9 |
-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: A1 / Wavelength: 0.91 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: May 17, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91 Å / Relative weight: 1 |
Reflection | Resolution: 2.75→15 Å / Num. obs: 29142 / % possible obs: 98.1 % / Redundancy: 2.9 % / Biso Wilson estimate: 37.2 Å2 / Rsym value: 0.11 / Net I/σ(I): 20.4 |
Reflection shell | Resolution: 2.75→2.85 Å / Redundancy: 2.7 % / Mean I/σ(I) obs: 4.3 / % possible all: 97.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2POH Resolution: 2.75→15.862 Å / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 28.38 / Stereochemistry target values: TWIN_LSQ_F
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.75→15.862 Å
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Refine LS restraints |
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LS refinement shell |
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