+Open data
-Basic information
Entry | Database: PDB / ID: 4zm8 | |||||||||
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Title | Crystal Structure of Sialostatin L | |||||||||
Components | Putative secreted cystatin | |||||||||
Keywords | Hydrolase inhibitor / cystatin / tick / protease inhibitor | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Ixodes scapularis (black-legged tick) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.6762 Å | |||||||||
Authors | Andersen, J.F. / Kosyfakis, M. | |||||||||
Citation | Journal: Mol. Microbiol. / Year: 2010 Title: The crystal structures of two salivary cystatins from the tick Ixodes scapularis and the effect of these inhibitors on the establishment of Borrelia burgdorferi infection in a murine model. Authors: Kotsyfakis, M. / Horka, H. / Salat, J. / Andersen, J.F. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4zm8.cif.gz | 92.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4zm8.ent.gz | 75.2 KB | Display | PDB format |
PDBx/mmJSON format | 4zm8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zm/4zm8 ftp://data.pdbj.org/pub/pdb/validation_reports/zm/4zm8 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 12415.671 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ixodes scapularis (black-legged tick) / Plasmid: pET17b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pLysS / References: UniProt: Q8MVB6 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.82 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: 19% PEG 6000, 0.1 M sodium citrate, pH 5.3 / PH range: 5.3 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-BM / Wavelength: 0.97931 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Aug 2, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97931 Å / Relative weight: 1 |
Reflection | Resolution: 2.67→40.53 Å / Num. obs: 15048 / % possible obs: 99.6 % / Redundancy: 6 % / Rmerge(I) obs: 0.058 / Net I/σ(I): 18.8 |
Reflection shell | Resolution: 2.67→2.79 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.131 / Mean I/σ(I) obs: 14.8 / % possible all: 97.3 |
-Processing
Software |
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Refinement | Resolution: 2.6762→40.53 Å / SU ML: 0.35 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 27.99 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6762→40.53 Å
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Refine LS restraints |
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LS refinement shell |
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