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Open data
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Basic information
Entry | Database: PDB / ID: 4xes | ||||||
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Title | Structure of active-like neurotensin receptor | ||||||
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![]() | SIGNALING PROTEIN / HYDROLASE / membrane protein / G protein-coupled receptor / GPCR / neurotensin receptor / NTSR1 | ||||||
Function / homology | ![]() response to antipsychotic drug / Peptide ligand-binding receptors / neuropeptide receptor binding / G protein-coupled neurotensin receptor activity / inositol phosphate catabolic process / symmetric synapse / response to mineralocorticoid / D-aspartate import across plasma membrane / positive regulation of gamma-aminobutyric acid secretion / regulation of membrane depolarization ...response to antipsychotic drug / Peptide ligand-binding receptors / neuropeptide receptor binding / G protein-coupled neurotensin receptor activity / inositol phosphate catabolic process / symmetric synapse / response to mineralocorticoid / D-aspartate import across plasma membrane / positive regulation of gamma-aminobutyric acid secretion / regulation of membrane depolarization / L-glutamate import across plasma membrane / positive regulation of arachidonic acid secretion / regulation of respiratory gaseous exchange / neuron spine / positive regulation of inhibitory postsynaptic potential / neuropeptide hormone activity / hyperosmotic response / digestive tract development / negative regulation of systemic arterial blood pressure / negative regulation of release of sequestered calcium ion into cytosol / positive regulation of glutamate secretion / G alpha (q) signalling events / positive regulation of inositol phosphate biosynthetic process / temperature homeostasis / response to corticosterone / response to lipid / cellular response to lithium ion / detection of temperature stimulus involved in sensory perception of pain / response to axon injury / neuropeptide signaling pathway / axon terminus / viral release from host cell by cytolysis / transport vesicle / response to amphetamine / blood vessel diameter maintenance / peptidoglycan catabolic process / adult locomotory behavior / cellular response to dexamethasone stimulus / positive regulation of release of sequestered calcium ion into cytosol / cellular response to nerve growth factor stimulus / liver development / response to cocaine / dendritic shaft / learning / visual learning / terminal bouton / cytoplasmic side of plasma membrane / cell wall macromolecule catabolic process / lysozyme / response to estradiol / lysozyme activity / perikaryon / host cell cytoplasm / dendritic spine / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / receptor ligand activity / defense response to bacterium / positive regulation of apoptotic process / membrane raft / axon / negative regulation of gene expression / neuronal cell body / dendrite / protein-containing complex binding / positive regulation of gene expression / negative regulation of apoptotic process / cell surface / extracellular region / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Krumm, B.E. / White, J.F. / Shah, P. / Grisshammer, R. | ||||||
![]() | ![]() Title: Structural prerequisites for G-protein activation by the neurotensin receptor. Authors: Krumm, B.E. / White, J.F. / Shah, P. / Grisshammer, R. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 205.9 KB | Display | ![]() |
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PDB format | ![]() | 161.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 489.3 KB | Display | ![]() |
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Full document | ![]() | 494.8 KB | Display | |
Data in XML | ![]() | 20.2 KB | Display | |
Data in CIF | ![]() | 27.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4xeeC ![]() 4grvS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / Protein/peptide , 2 types, 2 molecules AB
#1: Protein | Mass: 60685.809 Da / Num. of mol.: 1 Fragment: UNP residues 43-396 (P20789), residues 2-161 (P00720) Mutation: A86L, E166A, G215A, V360A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: Ntsr1, Ntsr / Plasmid: pFASTBAC1 / Production host: ![]() |
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#2: Protein/peptide | Mass: 819.007 Da / Num. of mol.: 1 / Fragment: UNP residues 157-162 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
-Non-polymers , 5 types, 66 molecules ![](data/chem/img/CIT.gif)
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#3: Chemical | ChemComp-CIT / | ||||||
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#4: Chemical | ChemComp-PEG / #5: Chemical | #6: Chemical | #7: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.04 Å3/Da / Density % sol: 59.56 % |
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Crystal grow | Temperature: 293 K / Method: lipidic cubic phase Details: 19.8-23.4% PEG400, 80 mM HEPES, 50 mM lithium citrate, 2 mM TCEP PH range: 7.0-7.4 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 1, 2014 |
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→34.2 Å / Num. obs: 22591 / % possible obs: 99.5 % / Redundancy: 7.2 % / Biso Wilson estimate: 42.4 Å2 / Rmerge(I) obs: 0.15 / Net I/σ(I): 10.8 |
Reflection shell | Resolution: 2.6→2.72 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.75 / Mean I/σ(I) obs: 1.5 / % possible all: 98.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 4GRV Resolution: 2.6→34 Å / Cor.coef. Fo:Fc: 0.935 / Cor.coef. Fo:Fc free: 0.883 / SU B: 28.246 / SU ML: 0.287 / Cross valid method: THROUGHOUT / ESU R: 0.474 / ESU R Free: 0.312 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 57.625 Å2
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Refinement step | Cycle: 1 / Resolution: 2.6→34 Å
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