+Open data
-Basic information
Entry | Database: PDB / ID: 4bb7 | ||||||
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Title | Crystal structure of the yeast Rsc2 BAH domain | ||||||
Components | CHROMATIN STRUCTURE-REMODELING COMPLEX SUBUNIT RSC2 | ||||||
Keywords | TRANSCRIPTION / DNA BINDING | ||||||
Function / homology | Function and homology information regulation of sporulation resulting in formation of a cellular spore / plasmid maintenance / nucleosome disassembly / RSC-type complex / UV-damage excision repair / sister chromatid cohesion / sporulation resulting in formation of a cellular spore / chromosome segregation / transcription elongation by RNA polymerase II / double-strand break repair via homologous recombination ...regulation of sporulation resulting in formation of a cellular spore / plasmid maintenance / nucleosome disassembly / RSC-type complex / UV-damage excision repair / sister chromatid cohesion / sporulation resulting in formation of a cellular spore / chromosome segregation / transcription elongation by RNA polymerase II / double-strand break repair via homologous recombination / double-strand break repair via nonhomologous end joining / chromatin remodeling / chromatin binding Similarity search - Function | ||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Chambers, A.L. / Pearl, L.H. / Oliver, A.W. / Downs, J.A. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2013 Title: The Bah Domain of Rsc2 is a Histone H3 Binding Domain. Authors: Chambers, A.L. / Pearl, L.H. / Oliver, A.W. / Downs, J.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4bb7.cif.gz | 212.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4bb7.ent.gz | 168.3 KB | Display | PDB format |
PDBx/mmJSON format | 4bb7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bb/4bb7 ftp://data.pdbj.org/pub/pdb/validation_reports/bb/4bb7 | HTTPS FTP |
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-Related structure data
Related structure data | 1w4sS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 30105.117 Da / Num. of mol.: 4 / Fragment: DOMAIN, RESIDUES 401-641 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PTWO-E / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA2 / References: UniProt: Q06488 #2: Chemical | ChemComp-SO4 / #3: Chemical | ChemComp-CL / #4: Chemical | ChemComp-GOL / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.22 % / Description: NONE |
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Crystal grow | Details: 100 MM HEPES.NAOH PH 7.5, 0.2 M (NH4)2SO4, 20-30% W/V PEG 3350 |
-Data collection
Diffraction | Mean temperature: 199 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.9796 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Apr 10, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9796 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→45 Å / Num. obs: 42935 / % possible obs: 98.7 % / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Biso Wilson estimate: 25.45 Å2 / Rmerge(I) obs: 0.14 / Net I/σ(I): 5.8 |
Reflection shell | Resolution: 2.4→2.53 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.42 / Mean I/σ(I) obs: 2.7 / % possible all: 99 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1W4S Resolution: 2.4→44.493 Å / SU ML: 0.4 / σ(F): 2.01 / Phase error: 30.04 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 61.745 Å2 / ksol: 0.401 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.4→44.493 Å
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Refine LS restraints |
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LS refinement shell |
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