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Open data
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Basic information
Entry | Database: PDB / ID: 2c1t | ||||||
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Title | Structure of the Kap60p:Nup2 complex | ||||||
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![]() | PROTEIN TRANSPORT/MEMBRANE PROTEIN / ARMADILLO REPEAT / KARYOPHERIN RECYCLING / NLS RELEASE / NUCEAR IMPORT / NUCLEAR PROTEIN / NUCLEOPORIN / PROTEIN TRANSPORT / NUCLEAR TRANSPORT-COMPLEX / NUCLEAR PORE COMPLEX / PHOSPHORYLATION / TRANSLOCATION / PROTEIN TRANSPORT-MEMBRANE PROTEIN complex | ||||||
Function / homology | ![]() proteasome localization / mRNA export from nucleus in response to heat stress / protein localization to nuclear inner membrane / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore cytoplasmic filaments / import into nucleus / Transport of Mature mRNA derived from an Intron-Containing Transcript / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / Regulation of HSF1-mediated heat shock response / nuclear pore nuclear basket ...proteasome localization / mRNA export from nucleus in response to heat stress / protein localization to nuclear inner membrane / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore cytoplasmic filaments / import into nucleus / Transport of Mature mRNA derived from an Intron-Containing Transcript / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / Regulation of HSF1-mediated heat shock response / nuclear pore nuclear basket / SUMOylation of SUMOylation proteins / importin-alpha family protein binding / NLS-dependent protein nuclear import complex / SUMOylation of RNA binding proteins / structural constituent of nuclear pore / protein targeting to membrane / SUMOylation of chromatin organization proteins / nucleocytoplasmic transport / silent mating-type cassette heterochromatin formation / nuclear import signal receptor activity / poly(A)+ mRNA export from nucleus / nuclear localization sequence binding / NLS-bearing protein import into nucleus / subtelomeric heterochromatin formation / nuclear pore / protein export from nucleus / GTPase activator activity / small GTPase binding / protein import into nucleus / disordered domain specific binding / nuclear envelope / nuclear membrane / chromosome, telomeric region / protein-containing complex binding / perinuclear region of cytoplasm / protein-containing complex / mitochondrion / nucleoplasm / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Matsuura, Y. / Stewart, M. | ||||||
![]() | ![]() Title: Nup50/Npap60 Function in Nuclear Import Complex Disassembly and Importin Recycling Authors: Matsuura, Y. / Stewart, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 190.6 KB | Display | ![]() |
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PDB format | ![]() | 151.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 387.3 KB | Display | ![]() |
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Full document | ![]() | 402.5 KB | Display | |
Data in XML | ![]() | 18.7 KB | Display | |
Data in CIF | ![]() | 30 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2c1mC ![]() 1ee4S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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Components
#1: Protein | Mass: 50189.902 Da / Num. of mol.: 2 / Fragment: ARM DOMAIN, RESIDUES 88-541 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #2: Protein | Mass: 5813.649 Da / Num. of mol.: 2 / Fragment: RESIDUES 1-51 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #3: Water | ChemComp-HOH / | Compound details | IMPORTIN ALPHA SUBUNIT: SPECIFICALLY BINDS TO SUBSTRATES CONTAINING NLS MOTIF AND PROMOTES DOCKING ...IMPORTIN ALPHA SUBUNIT: SPECIFICAL | Sequence details | RESIDUES 1 TO 87 REMOVED. Y397D INTRODUCED | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 49 % |
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Crystal grow | pH: 6.8 Details: EXPERIMENTAL DETALS GIVEN IN ENTRY 1UN0 AND IN MATSUURA ET AL, EMBO J, 22, 5358, 2003., pH 6.80 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Apr 21, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→20 Å / Num. obs: 29670 / % possible obs: 97.5 % / Redundancy: 2.3 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 10 |
Reflection shell | Resolution: 2.6→2.74 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.53 / Mean I/σ(I) obs: 1.8 / % possible all: 98.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1EE4 Resolution: 2.6→20 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.914 / SU B: 10.848 / SU ML: 0.229 / Cross valid method: THROUGHOUT / ESU R: 0.673 / ESU R Free: 0.31 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.39 Å2
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Refinement step | Cycle: LAST / Resolution: 2.6→20 Å
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Refine LS restraints |
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