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Open data
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Basic information
Entry | Database: PDB / ID: 1bk5 | ||||||
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Title | KARYOPHERIN ALPHA FROM SACCHAROMYCES CEREVISIAE | ||||||
![]() | KARYOPHERIN ALPHA | ||||||
![]() | PROTEIN TRANSPORT / NLS NUCLEAR IMPORT / ARMADILLO REPEAT CONTAINING PROTEIN | ||||||
Function / homology | ![]() proteasome localization / import into nucleus / NLS-dependent protein nuclear import complex / protein targeting to membrane / nuclear import signal receptor activity / nuclear localization sequence binding / NLS-bearing protein import into nucleus / protein import into nucleus / disordered domain specific binding / nuclear envelope ...proteasome localization / import into nucleus / NLS-dependent protein nuclear import complex / protein targeting to membrane / nuclear import signal receptor activity / nuclear localization sequence binding / NLS-bearing protein import into nucleus / protein import into nucleus / disordered domain specific binding / nuclear envelope / protein-containing complex binding / perinuclear region of cytoplasm / protein-containing complex / nucleoplasm / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Conti, E. / Uy, M. / Leighton, L. / Blobel, G. / Kuriyan, J. | ||||||
![]() | ![]() Title: Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha. Authors: Conti, E. / Uy, M. / Leighton, L. / Blobel, G. / Kuriyan, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 174 KB | Display | ![]() |
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PDB format | ![]() | 137.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 373.1 KB | Display | ![]() |
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Full document | ![]() | 391.1 KB | Display | |
Data in XML | ![]() | 18.6 KB | Display | |
Data in CIF | ![]() | 29.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.99774, 0.06554, -0.01504), Vector: |
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Components
#1: Protein | Mass: 46718.426 Da / Num. of mol.: 2 / Fragment: ARMADILLO DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: W303 / Cell line: BL21 / Gene: SRP1 / Plasmid: PPROEX-HTB / Species (production host): Escherichia coli / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 50 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.0 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: BRANDEIS / Detector: CCD / Date: Feb 1, 1998 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→19.5 Å / Num. obs: 48255 / % possible obs: 95 % / Rsym value: 0.065 / Net I/σ(I): 13.6 |
Reflection shell | Resolution: 2.2→2.28 Å / Mean I/σ(I) obs: 2.8 / Rsym value: 0.299 / % possible all: 95 |
Reflection | *PLUS Num. measured all: 525332 / Rmerge(I) obs: 0.065 |
Reflection shell | *PLUS % possible obs: 95 % / Rmerge(I) obs: 0.299 |
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Processing
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Refinement | Method to determine structure: MIR (MIR) / Resolution: 2.2→19.5 Å / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.2→19.5 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.22 Å / Total num. of bins used: 38
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.3 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.356 |