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- PDB-1l2j: Human Estrogen Receptor beta Ligand-binding Domain in Complex wit... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1l2j | ||||||
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Title | Human Estrogen Receptor beta Ligand-binding Domain in Complex with (R,R)-5,11-cis-diethyl-5,6,11,12-tetrahydrochrysene-2,8-diol | ||||||
![]() | ESTROGEN RECEPTOR BETA | ||||||
![]() | transcription receptor / nuclear receptor / transcription factor / estrogen / antagonist | ||||||
Function / homology | ![]() receptor antagonist activity / nuclear steroid receptor activity / nuclear estrogen receptor activity / estrogen receptor signaling pathway / cellular response to estrogen stimulus / estrogen response element binding / steroid binding / ESR-mediated signaling / cellular response to estradiol stimulus / negative regulation of cell growth ...receptor antagonist activity / nuclear steroid receptor activity / nuclear estrogen receptor activity / estrogen receptor signaling pathway / cellular response to estrogen stimulus / estrogen response element binding / steroid binding / ESR-mediated signaling / cellular response to estradiol stimulus / negative regulation of cell growth / positive regulation of DNA-binding transcription factor activity / Nuclear Receptor transcription pathway / Constitutive Signaling by Aberrant PI3K in Cancer / nuclear receptor activity / PIP3 activates AKT signaling / cell-cell signaling / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / Extra-nuclear estrogen signaling / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / intracellular membrane-bounded organelle / regulation of DNA-templated transcription / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / enzyme binding / negative regulation of transcription by RNA polymerase II / signal transduction / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / zinc ion binding / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shiau, A.K. / Barstad, D. / Radek, J.T. / Meyers, M.J. / Nettles, K.W. / Katzenellenbogen, B.S. / Katzenellenbogen, J.A. / Agard, D.A. / Greene, G.L. | ||||||
![]() | ![]() Title: Structural characterization of a subtype-selective ligand reveals a novel mode of estrogen receptor antagonism. Authors: Shiau, A.K. / Barstad, D. / Radek, J.T. / Meyers, M.J. / Nettles, K.W. / Katzenellenbogen, B.S. / Katzenellenbogen, J.A. / Agard, D.A. / Greene, G.L. #1: ![]() Title: Structure of the Ligand-binding Domain of Oestrogen Receptor Beta in the Presence of a Partial Agonist and a Full Antagonist Authors: Pike, A.C.W. / Brzozowski, A.M. / Hubbard, R.E. / Bonn, T. / Thorsell, A.G. / Engstrom, O. / Ljunggren, J. / Gustafsson, J.A. / Carlquist, M. #2: ![]() Title: Estrogen Receptor Subtype-selective Ligands: Asymmetric Synthesis and Biological Evaluation of cis- and trans-5,11-dialkyl-5,6,11,12-tetrahydrochrysenes Authors: Meyers, M.J. / Sun, J. / Carlson, K.E. / Katzenellenbogen, B.S. / Katzenellenbogen, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 101.9 KB | Display | ![]() |
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PDB format | ![]() | 76.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1001.8 KB | Display | ![]() |
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Full document | ![]() | 1008.6 KB | Display | |
Data in XML | ![]() | 18.2 KB | Display | |
Data in CIF | ![]() | 23.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1l2iC ![]() 1a52S ![]() 1ereS ![]() 1errS ![]() 3erdS ![]() 3ertS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Details | The biological assembly is a homodimer which is not observed in the crystal. |
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Components
#1: Protein | Mass: 30571.119 Da / Num. of mol.: 2 / Fragment: ligand-binding domain (residues 256-505) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 67.41 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: 1.5-1.75 M Ammonium sulfate, 100 mM Sodium Acetate pH 4.8-5.2 294-296 K, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 21-23 ℃ / PH range low: 5.2 / PH range high: 4.8 | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 14, 1999 |
Radiation | Monochromator: Double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.07 Å / Relative weight: 1 |
Reflection | Resolution: 2.95→49.6 Å / Num. all: 14895 / Num. obs: 14895 / % possible obs: 99.7 % / Observed criterion σ(I): -3 / Redundancy: 4.3 % / Biso Wilson estimate: 46.7 Å2 / Rsym value: 0.045 / Net I/σ(I): 23.3 |
Reflection shell | Resolution: 2.95→3.06 Å / Mean I/σ(I) obs: 3.4 / Num. unique all: 1507 / Rsym value: 0.302 / % possible all: 100 |
Reflection | *PLUS Num. measured all: 64540 / Rmerge(I) obs: 0.045 |
Reflection shell | *PLUS % possible obs: 100 % / Rmerge(I) obs: 0.302 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRIES 1A52, 1ERE, 1ERR, 3ERD, 3ERT Resolution: 2.95→49.6 Å / Rfactor Rfree error: 0.011 / Data cutoff high absF: 10000 / Isotropic thermal model: Restrained / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber Details: The structure was refined against data which had been sharpened with a -55 A**2 correction factor.
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Solvent computation | Solvent model: flat model / Bsol: 33.127 Å2 / ksol: 0.333695 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 40.08 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.95→49.6 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: constrained | ||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.95→3.06 Å / Rfactor Rfree error: 0.044 / Total num. of bins used: 10
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Xplor file |
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Refinement | *PLUS Rfactor obs: 0.259 / Rfactor Rfree: 0.299 / Rfactor Rwork: 0.259 | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.351 / Rfactor Rwork: 0.34 / Rfactor obs: 0.34 |