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- PDB-4v6e: Crystal structure of the E. coli 70S ribosome in an intermediate ... -

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Basic information

Entry
Database: PDB / ID: 4v6e
TitleCrystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting
Components
  • (16S rRNA) x 2
  • (30S ribosomal protein ...) x 20
  • (50S ribosomal protein ...) x 29
  • (5S rRNA) x 2
  • 23S rRNA
  • P-site tRNA ASL fragment
  • messenger RNA
KeywordsRIBOSOME / ribosome structure / protein-RNA complex / Ribonucleoprotein / Ribosomal protein / RNA-binding / rRNA-binding / Antibiotic resistance / Repressor / Transcription / Transcription regulation / Transcription termination / Translation regulation / Acetylation / tRNA-binding / Methylation / Endonuclease / Hydrolase / Nuclease
Function / homology
Function and homology information


stringent response / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / RNA-binding transcription regulator activity ...stringent response / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / positive regulation of ribosome biogenesis / RNA-binding transcription regulator activity / translational termination / negative regulation of cytoplasmic translation / four-way junction DNA binding / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / regulation of mRNA stability / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / assembly of large subunit precursor of preribosome / positive regulation of RNA splicing / ribosome assembly / regulation of DNA-templated transcription elongation / transcription elongation factor complex / cytosolic ribosome assembly / response to reactive oxygen species / DNA endonuclease activity / transcription antitermination / regulation of cell growth / DNA-templated transcription termination / response to radiation / maintenance of translational fidelity / mRNA 5'-UTR binding / ribosome biogenesis / large ribosomal subunit / regulation of translation / ribosome binding / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytoplasm / cytosol
Similarity search - Function
Ribosomal protein L11, bacterial-type / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 ...Ribosomal protein L11, bacterial-type / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / : / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L5, bacterial-type / Ribosomal protein L36 / Ribosomal protein S4, bacterial-type / Ribosomal protein L36 superfamily / Ribosomal protein L36 / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L16 / Ribosomal protein S18, conserved site / Ribosomal protein L28/L24 / Ribosomal protein S18 signature. / Ribosomal protein L30, bacterial-type
Similarity search - Domain/homology
RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 ...RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL36A / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL25
Similarity search - Component
Biological speciesEscherichia coli K-12 (bacteria)
Escherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 3.712 Å
AuthorsZhang, W. / Dunkle, J.A. / Cate, J.H.D.
CitationJournal: Science / Year: 2009
Title: Structures of the ribosome in intermediate States of ratcheting.
Authors: Zhang, W. / Dunkle, J.A. / Cate, J.H.
History
DepositionJun 28, 2009Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 9, 2014Provider: repository / Type: Initial release
SupersessionDec 10, 2014ID: 3I1Z, 3I20, 3I21, 3I22
Revision 1.1Dec 10, 2014Group: Other
Revision 1.2Oct 16, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature / pdbx_struct_conn_angle / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_comp_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_atom_id / _pdbx_struct_conn_angle.ptnr2_label_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
AB: 30S ribosomal protein S2
AC: 30S ribosomal protein S3
AD: 30S ribosomal protein S4
AE: 30S ribosomal protein S5
AF: 30S ribosomal protein S6
AG: 30S ribosomal protein S7
AH: 30S ribosomal protein S8
AI: 30S ribosomal protein S9
AJ: 30S ribosomal protein S10
AK: 30S ribosomal protein S11
AL: 30S ribosomal protein S12
AM: 30S ribosomal protein S13
AN: 30S ribosomal protein S14
AO: 30S ribosomal protein S15
AP: 30S ribosomal protein S16
AQ: 30S ribosomal protein S17
AR: 30S ribosomal protein S18
AS: 30S ribosomal protein S19
AT: 30S ribosomal protein S20
AU: 30S ribosomal protein S21
AA: 16S rRNA
AV: P-site tRNA ASL fragment
AW: messenger RNA
AX: P-site tRNA ASL fragment
BA: 23S rRNA
BB: 5S rRNA
BC: 50S ribosomal protein L2
BD: 50S ribosomal protein L3
BE: 50S ribosomal protein L4
BF: 50S ribosomal protein L5
BG: 50S ribosomal protein L6
BH: 50S ribosomal protein L9
BI: 50S ribosomal protein L11
BJ: 50S ribosomal protein L13
BK: 50S ribosomal protein L14
BL: 50S ribosomal protein L15
BM: 50S ribosomal protein L16
BN: 50S ribosomal protein L17
BO: 50S ribosomal protein L18
BP: 50S ribosomal protein L19
BQ: 50S ribosomal protein L20
BR: 50S ribosomal protein L21
BS: 50S ribosomal protein L22
BT: 50S ribosomal protein L23
BU: 50S ribosomal protein L24
BV: 50S ribosomal protein L25
BW: 50S ribosomal protein L27
BX: 50S ribosomal protein L28
BY: 50S ribosomal protein L29
BZ: 50S ribosomal protein L30
B0: 50S ribosomal protein L32
B1: 50S ribosomal protein L33
B2: 50S ribosomal protein L34
B3: 50S ribosomal protein L35
B4: 50S ribosomal protein L36
CB: 30S ribosomal protein S2
CC: 30S ribosomal protein S3
CD: 30S ribosomal protein S4
CE: 30S ribosomal protein S5
CF: 30S ribosomal protein S6
CG: 30S ribosomal protein S7
CH: 30S ribosomal protein S8
CI: 30S ribosomal protein S9
CJ: 30S ribosomal protein S10
CK: 30S ribosomal protein S11
CL: 30S ribosomal protein S12
CM: 30S ribosomal protein S13
CN: 30S ribosomal protein S14
CO: 30S ribosomal protein S15
CP: 30S ribosomal protein S16
CQ: 30S ribosomal protein S17
CR: 30S ribosomal protein S18
CS: 30S ribosomal protein S19
CT: 30S ribosomal protein S20
CU: 30S ribosomal protein S21
CA: 16S rRNA
CV: P-site tRNA ASL fragment
CW: messenger RNA
CX: P-site tRNA ASL fragment
DA: 23S rRNA
DB: 5S rRNA
DC: 50S ribosomal protein L2
DD: 50S ribosomal protein L3
DE: 50S ribosomal protein L4
DF: 50S ribosomal protein L5
DG: 50S ribosomal protein L6
DH: 50S ribosomal protein L9
DI: 50S ribosomal protein L11
DJ: 50S ribosomal protein L13
DK: 50S ribosomal protein L14
DL: 50S ribosomal protein L15
DM: 50S ribosomal protein L16
DN: 50S ribosomal protein L17
DO: 50S ribosomal protein L18
DP: 50S ribosomal protein L19
DQ: 50S ribosomal protein L20
DR: 50S ribosomal protein L21
DS: 50S ribosomal protein L22
DT: 50S ribosomal protein L23
DU: 50S ribosomal protein L24
DV: 50S ribosomal protein L25
DW: 50S ribosomal protein L27
DX: 50S ribosomal protein L28
DY: 50S ribosomal protein L29
DZ: 50S ribosomal protein L30
D0: 50S ribosomal protein L32
D1: 50S ribosomal protein L33
D2: 50S ribosomal protein L34
D3: 50S ribosomal protein L35
D4: 50S ribosomal protein L36
hetero molecules


Theoretical massNumber of molelcules
Total (without water)4,319,807475
Polymers4,310,853110
Non-polymers8,954365
Water30,9501718
1
AB: 30S ribosomal protein S2
AC: 30S ribosomal protein S3
AD: 30S ribosomal protein S4
AE: 30S ribosomal protein S5
AF: 30S ribosomal protein S6
AG: 30S ribosomal protein S7
AH: 30S ribosomal protein S8
AI: 30S ribosomal protein S9
AJ: 30S ribosomal protein S10
AK: 30S ribosomal protein S11
AL: 30S ribosomal protein S12
AM: 30S ribosomal protein S13
AN: 30S ribosomal protein S14
AO: 30S ribosomal protein S15
AP: 30S ribosomal protein S16
AQ: 30S ribosomal protein S17
AR: 30S ribosomal protein S18
AS: 30S ribosomal protein S19
AT: 30S ribosomal protein S20
AU: 30S ribosomal protein S21
AA: 16S rRNA
AV: P-site tRNA ASL fragment
AW: messenger RNA
AX: P-site tRNA ASL fragment
BA: 23S rRNA
BB: 5S rRNA
BC: 50S ribosomal protein L2
BD: 50S ribosomal protein L3
BE: 50S ribosomal protein L4
BF: 50S ribosomal protein L5
BG: 50S ribosomal protein L6
BH: 50S ribosomal protein L9
BI: 50S ribosomal protein L11
BJ: 50S ribosomal protein L13
BK: 50S ribosomal protein L14
BL: 50S ribosomal protein L15
BM: 50S ribosomal protein L16
BN: 50S ribosomal protein L17
BO: 50S ribosomal protein L18
BP: 50S ribosomal protein L19
BQ: 50S ribosomal protein L20
BR: 50S ribosomal protein L21
BS: 50S ribosomal protein L22
BT: 50S ribosomal protein L23
BU: 50S ribosomal protein L24
BV: 50S ribosomal protein L25
BW: 50S ribosomal protein L27
BX: 50S ribosomal protein L28
BY: 50S ribosomal protein L29
BZ: 50S ribosomal protein L30
B0: 50S ribosomal protein L32
B1: 50S ribosomal protein L33
B2: 50S ribosomal protein L34
B3: 50S ribosomal protein L35
B4: 50S ribosomal protein L36
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,160,611240
Polymers2,156,07455
Non-polymers4,538185
Water30617
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
CB: 30S ribosomal protein S2
CC: 30S ribosomal protein S3
CD: 30S ribosomal protein S4
CE: 30S ribosomal protein S5
CF: 30S ribosomal protein S6
CG: 30S ribosomal protein S7
CH: 30S ribosomal protein S8
CI: 30S ribosomal protein S9
CJ: 30S ribosomal protein S10
CK: 30S ribosomal protein S11
CL: 30S ribosomal protein S12
CM: 30S ribosomal protein S13
CN: 30S ribosomal protein S14
CO: 30S ribosomal protein S15
CP: 30S ribosomal protein S16
CQ: 30S ribosomal protein S17
CR: 30S ribosomal protein S18
CS: 30S ribosomal protein S19
CT: 30S ribosomal protein S20
CU: 30S ribosomal protein S21
CA: 16S rRNA
CV: P-site tRNA ASL fragment
CW: messenger RNA
CX: P-site tRNA ASL fragment
DA: 23S rRNA
DB: 5S rRNA
DC: 50S ribosomal protein L2
DD: 50S ribosomal protein L3
DE: 50S ribosomal protein L4
DF: 50S ribosomal protein L5
DG: 50S ribosomal protein L6
DH: 50S ribosomal protein L9
DI: 50S ribosomal protein L11
DJ: 50S ribosomal protein L13
DK: 50S ribosomal protein L14
DL: 50S ribosomal protein L15
DM: 50S ribosomal protein L16
DN: 50S ribosomal protein L17
DO: 50S ribosomal protein L18
DP: 50S ribosomal protein L19
DQ: 50S ribosomal protein L20
DR: 50S ribosomal protein L21
DS: 50S ribosomal protein L22
DT: 50S ribosomal protein L23
DU: 50S ribosomal protein L24
DV: 50S ribosomal protein L25
DW: 50S ribosomal protein L27
DX: 50S ribosomal protein L28
DY: 50S ribosomal protein L29
DZ: 50S ribosomal protein L30
D0: 50S ribosomal protein L32
D1: 50S ribosomal protein L33
D2: 50S ribosomal protein L34
D3: 50S ribosomal protein L35
D4: 50S ribosomal protein L36
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,159,196235
Polymers2,154,78055
Non-polymers4,416180
Water37821
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)210.952, 433.082, 624.495
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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30S ribosomal protein ... , 20 types, 40 molecules ABCBACCCADCDAECEAFCFAGCGAHCHAICIAJCJAKCKALCLAMCMANCNAOCOAPCP...

#1: Protein 30S ribosomal protein S2


Mass: 26781.670 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7V0
#2: Protein 30S ribosomal protein S3


Mass: 26031.316 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7V3
#3: Protein 30S ribosomal protein S4


Mass: 23514.199 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7V8
#4: Protein 30S ribosomal protein S5


Mass: 17629.398 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7W1
#5: Protein 30S ribosomal protein S6 / 30S ribosomal protein S6 / fully modified isoform / 30S ribosomal protein S6 / non-modified isoform


Mass: 15727.512 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P02358
#6: Protein 30S ribosomal protein S7


Mass: 20055.156 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P02359
#7: Protein 30S ribosomal protein S8


Mass: 14146.557 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7W7
#8: Protein 30S ribosomal protein S9


Mass: 14886.270 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7X3
#9: Protein 30S ribosomal protein S10


Mass: 11755.597 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7R5
#10: Protein 30S ribosomal protein S11


Mass: 13870.975 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7R9
#11: Protein 30S ribosomal protein S12


Mass: 13768.157 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7S3
#12: Protein 30S ribosomal protein S13


Mass: 13128.467 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7S9
#13: Protein 30S ribosomal protein S14


Mass: 11606.560 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0AG59
#14: Protein 30S ribosomal protein S15


Mass: 10290.816 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0ADZ4
#15: Protein 30S ribosomal protein S16


Mass: 9207.572 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7T3
#16: Protein 30S ribosomal protein S17


Mass: 9724.491 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0AG63
#17: Protein 30S ribosomal protein S18


Mass: 9005.472 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7T7
#18: Protein 30S ribosomal protein S19


Mass: 10455.355 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7U3
#19: Protein 30S ribosomal protein S20


Mass: 9708.464 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7U7
#20: Protein 30S ribosomal protein S21


Mass: 8524.039 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P68679

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RNA chain , 7 types, 12 molecules AAAVAXCVCXAWCWBADABBCADB

#21: RNA chain 16S rRNA


Mass: 496892.375 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli)
#22: RNA chain
P-site tRNA ASL fragment


Mass: 5466.324 Da / Num. of mol.: 4 / Fragment: RNA hairpin / Source method: obtained synthetically
#23: RNA chain messenger RNA


Mass: 1792.037 Da / Num. of mol.: 2 / Source method: obtained synthetically
#24: RNA chain 23S rRNA


Mass: 941306.188 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli)
#25: RNA chain 5S rRNA


Mass: 38177.762 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli)
#55: RNA chain 16S rRNA


Mass: 495927.719 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli)
#56: RNA chain 5S rRNA


Mass: 37848.555 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli)

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50S ribosomal protein ... , 29 types, 58 molecules BCDCBDDDBEDEBFDFBGDGBHDHBIDIBJDJBKDKBLDLBMDMBNDNBODOBPDPBQDQ...

#26: Protein 50S ribosomal protein L2


Mass: 29923.619 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P60422
#27: Protein 50S ribosomal protein L3


Mass: 22277.535 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P60438
#28: Protein 50S ribosomal protein L4


Mass: 22121.566 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P60723
#29: Protein 50S ribosomal protein L5


Mass: 20333.611 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P62399
#30: Protein 50S ribosomal protein L6


Mass: 18932.791 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0AG55
#31: Protein 50S ribosomal protein L9


Mass: 15789.020 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7R1
#32: Protein 50S ribosomal protein L11


Mass: 14894.362 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7J7
#33: Protein 50S ribosomal protein L13


Mass: 16050.606 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0AA10
#34: Protein 50S ribosomal protein L14


Mass: 13565.067 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0ADY3
#35: Protein 50S ribosomal protein L15


Mass: 15008.471 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P02413
#36: Protein 50S ribosomal protein L16


Mass: 15312.269 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0ADY7
#37: Protein 50S ribosomal protein L17


Mass: 14393.657 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0AG44
#38: Protein 50S ribosomal protein L18


Mass: 12794.668 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0C018
#39: Protein 50S ribosomal protein L19


Mass: 13159.278 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7K6
#40: Protein 50S ribosomal protein L20


Mass: 13528.024 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7L3
#41: Protein 50S ribosomal protein L21


Mass: 11586.374 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0AG48
#42: Protein 50S ribosomal protein L22


Mass: 12253.359 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P61175
#43: Protein 50S ribosomal protein L23


Mass: 11222.160 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0ADZ0
#44: Protein 50S ribosomal protein L24


Mass: 11339.250 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P60624
#45: Protein 50S ribosomal protein L25


Mass: 10713.465 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P68919
#46: Protein 50S ribosomal protein L27


Mass: 9146.540 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7L8
#47: Protein 50S ribosomal protein L28


Mass: 9027.551 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7M2
#48: Protein 50S ribosomal protein L29


Mass: 7286.464 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7M6
#49: Protein 50S ribosomal protein L30


Mass: 6554.820 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0AG51
#50: Protein 50S ribosomal protein L32


Mass: 6463.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7N4
#51: Protein 50S ribosomal protein L33


Mass: 6388.631 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7N9
#52: Protein/peptide 50S ribosomal protein L34


Mass: 5397.463 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7P5
#53: Protein 50S ribosomal protein L35 / Ribosomal protein A


Mass: 7313.032 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7Q1
#54: Protein/peptide 50S ribosomal protein L36 / Ribosomal protein B


Mass: 4377.390 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli K-12 (bacteria) / References: UniProt: P0A7Q6

-
Non-polymers , 3 types, 2083 molecules

#57: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 363 / Source method: obtained synthetically / Formula: Mg
#58: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#59: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1718 / Source method: isolated from a natural source / Formula: H2O

-
Details

Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

-
Sample preparation

CrystalDensity Matthews: 3.31 Å3/Da / Density % sol: 62.83 %
Crystal growTemperature: 291 K / Method: microbatch / pH: 7
Details: 4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, pH 7.0, microbatch, temperature 291K
Components of the solutions
IDNameCrystal-IDSol-ID
1PEG 800011
2MPD11
3MgCl211
4NH4Cl11
5putrescine11
6spermidine11
7PEG 800012
8MPD12
9MgCl212
10NH4Cl12
11putrescine12
12spermidine12

-
Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.97918 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 15, 2008
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 3.7→100 Å / Num. obs: 452913 / % possible obs: 75.9 % / Redundancy: 2.2 % / Rmerge(I) obs: 0.078 / Χ2: 1.378 / Net I/σ(I): 11.4
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allΧ2Diffraction-ID% possible all
3.7-3.761.20.64121501.29141
3.76-3.831.30.528203371.022168.6
3.83-3.911.20.574128921.298143.5
3.91-3.991.40.41217141.122173.3
3.99-4.071.30.361184520.986162.2
4.07-4.171.40.346194501.01165.8
4.17-4.271.50.305206781.042169.5
4.27-4.391.60.274216181.132172.8
4.39-4.521.80.261223641.122175.5
4.52-4.661.90.232231521.189177.7
4.66-4.8320.219235891.294179.5
4.83-5.022.10.197240061.31180.5
5.02-5.252.20.176241951.408181.3
5.25-5.532.30.155246781.397182.7
5.53-5.872.40.139251541.372184.3
5.87-6.332.50.125257641.296186.1
6.33-6.962.80.105261601.521187.2
6.96-7.973.30.075281401.364193.6
7.97-10.043.40.054292061.396196.3
10.04-1003.40.039292141.736194.2

-
Processing

Software
NameVersionClassificationNB
SCALEPACKdata scaling
PHENIXrefinement
PDB_EXTRACT3.006data extraction
PHASERphasing
DENZOdata reduction
RefinementResolution: 3.712→73.436 Å / Occupancy max: 1 / Occupancy min: 1 / SU ML: -0 / σ(F): 1.34 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.268 9161 2.02 %
Rwork0.227 --
obs0.228 452728 75.69 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 69.301 Å2 / ksol: 0.245 e/Å3
Displacement parametersBiso max: 298.69 Å2 / Biso mean: 163.57 Å2 / Biso min: 73.73 Å2
Baniso -1Baniso -2Baniso -3
1--11.963 Å20 Å20 Å2
2---6.686 Å20 Å2
3---18.649 Å2
Refinement stepCycle: LAST / Resolution: 3.712→73.436 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms18543 33745 43 208 52539
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.006310396
X-RAY DIFFRACTIONf_angle_d1.516465118
X-RAY DIFFRACTIONf_chiral_restr0.07958998
X-RAY DIFFRACTIONf_plane_restr0.00524798
X-RAY DIFFRACTIONf_dihedral_angle_d28.055161894
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3.712-3.7540.3231670.318454862144
3.754-3.7980.3321630.317302746538
3.798-3.8440.3463050.305151461545178
3.844-3.8930.3461890.3089718990750
3.893-3.9440.3691690.38457862644
3.944-3.9980.3263290.282157351606481
3.998-4.0550.3242300.28104421067254
4.055-4.1160.3162880.272143001458874
4.116-4.180.3132620.267124601272264
4.18-4.2490.2622480.264118171206561
4.249-4.3220.3043210.256154021572379
4.322-4.40.3153020.249142111451373
4.4-4.4850.282680.241132441351268
4.485-4.5770.2893270.244160451637282
4.577-4.6760.2663290.235154081573779
4.676-4.7850.2873310.234142261455773
4.785-4.9050.2613340.232164061674084
4.905-5.0370.2923270.224160721639983
5.037-5.1850.253210.216158791620081
5.185-5.3530.2593000.212154691576979
5.353-5.5440.2523480.203167161706486
5.544-5.7660.2583210.204163361665784
5.766-6.0280.2463480.199163501669883
6.028-6.3460.2643770.207173101768789
6.346-6.7430.2563560.209167641712086
6.743-7.2630.2523520.2177381809090
7.263-7.9930.2394030.196188091921296
7.993-9.1480.2483760.201191141949096
9.148-11.5190.2333730.195190831945696
11.519-73.450.2343970.218191541955194
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.5164-0.332-0.18651.1322-0.08120.71860.21540.65080.2677-0.6138-0.2353-0.4004-0.2621-0.173900.89350.09470.10810.55540.21960.5767-64.388213.8224-75.0261
20.0110.01150.03040.0553-0.00150.0343-0.04190.04850.02180.1812-0.0059-0.2824-0.18640.150600.88710.2668-0.23920.5168-0.03450.6996-87.551837.1141-29.4335
30.0457-0.05370.01930.04150.01980.0545-0.04870.11810.1981-0.11930.0118-0.0988-0.20730.165201.66220.16780.05721.74640.50511.8282-99.31461.8112-36.0925
40.0493-0.00720.00440.03260.00850.031-0.0072-0.0140.037-0.00650.02940.0111-0.18720.01901.93350.06580.10632.1970.32071.9101-80.801861.1208-43.1166
50.08480.0312-0.03010.14860.02160.10680.1398-0.2530.01360.19070.09860.0263-0.04650.111302.98030.0447-0.29462.34410.40293.0668-87.90186.2786-11.1782
60.01620.00490.00620.02940.0060.0184-0.0257-0.01570.00620.0333-0.02180.03320.0341-0.009401.54750.0251-0.04991.33230.04261.6133-119.782385.1955-10.0379
71.4038-0.6299-0.34580.92360.09070.892-0.1140.01320.03820.56950.13550.4971-0.1514-0.3541-01.26890.73460.25121.20540.13521.1185-148.50843.3414-4.1626
80.14990.0196-0.0170.12590.10630.13320.01260.27530.2646-0.34150.10730.3749-0.4205-0.3456-02.20450.5559-0.34892.5159-0.06562.3698-169.00727.4973-50.5435
90.9776-0.3936-0.04561.0467-0.10970.3465-0.3777-0.53510.77960.73370.4036-0.582-0.38970.1651-01.24780.3571-0.59050.5203-0.44721.1474-65.819739.0622-1.2688
100.17860.37730.30520.5758-0.34560.4562-0.00840.19660.0388-0.206-0.0927-0.3588-0.23420.0686-00.4681-0.0203-0.0480.27510.04830.4382-57.5662-5.7242-40.9487
111.2770.21710.24510.36080.24871.289-0.0065-0.3948-0.7150.48490.1017-0.48080.3030.573701.54690.049-0.60940.84140.20381.3724-29.627933.9126105.319
120.02270.0296-0.00310.05910.06490.08020.1151-0.2165-0.25350.04060.1336-0.24810.17370.2612-01.551-0.4873-0.65060.47120.1630.5224-41.999580.771577.0964
130.02120.01210.03140.025-0.02180.04090.05750.1278-0.0751-0.0720.0295-0.08560.00480.089501.25480.0409-0.46371.0855-0.20121.0829-51.679473.109153.5046
140.0214-0.00570.00730.0211-0.00640.022-0.09810.0098-0.0692-0.1091-0.0459-0.11410.16380.0778-01.5965-0.2498-0.45331.0068-0.08781.3138-34.778562.991358.0381
150.0712-0.0133-0.03110.075-0.03210.10670.05010.19980.1048-0.1904-0.059-0.1615-0.21690.0984-02.5245-0.2440.18082.3305-0.33541.5649-32.525691.990927.8488
160.01760.0061-0.00290.014-0.00250.0170.03060.02930.01420.01180.0070.0586-0.0563-0.058301.4781-0.0310.13531.3942-0.04041.2202-63.397398.255623.863
171.5521-0.12730.50160.9841-0.13360.89050.1607-0.34930.3379-0.0085-0.09980.8515-0.1976-0.724401.6971-0.5224-0.49831.1560.06331.3878-97.1822112.488559.222
180.15080.0152-0.0520.1642-0.14980.14780.14380.2155-0.2126-0.1196-0.17780.27480.0376-0.2828-02.7348-0.6902-0.50152.49280.23592.7109-125.976670.444374.3521
190.98880.10950.22790.7697-0.05320.39860.13380.2995-0.0819-0.2388-0.0408-0.5486-0.17820.4263-01.5324-0.5736-0.25330.917-0.10660.5874-16.3197105.669577.6494
200.1050.2521-0.07270.30960.29980.3449-0.1982-0.0862-0.35930.15890.3117-0.33710.28570.40802.0523-0.545-0.5361.38760.17690.9305-23.063668.1515125.2603
210.57860.0044-0.0710.48480.0210.4616-0.07-0.08560.0190.18330.0152-0.1035-0.02250.06030-0.3807-0.0183-0.2248-0.2858-0.2083-0.1692-66.4279-59.598915.6877
22-0.0737-0.01210.08490.119-0.2575-0.12120.22290.43512.0608-0.88090.0284-0.3239-2.32090.1549-02.00630.1023-0.30542.07280.2583.1352-140.1974-49.6891-62.5401
230.0032-0.0093-0.0024-0.00580.01530.00780.08930.10790.0264-0.13740.0077-0.0071-0.0819-0.0641-00.63990.273-0.03280.67190.030.4825-104.63-3.7098-1.7491
240.17890.0316-0.0580.1222-0.010.2052-0.0404-0.37620.50260.44290.1920.8978-0.938-1.0924-03.11710.069-0.04942.90780.05522.8701-95.678618.292380.2239
250.03990.00220.00360.02460.00030.0337-0.06550.18130.1877-0.1133-0.07630.0332-0.1559-0.019201.99220.0583-0.00871.7119-0.06141.9572-149.3888-27.1512-11.3879
260.0532-0.0470.00980.1123-0.00580.0598-0.05630.13620.75280.04750.1175-0.2454-1.3070.41800.6824-0.1694-0.06021.5306-0.1231.7299-166.4909-23.670816.7571
270.175-0.01820.01450.142-0.06820.08110.0361-0.0954-0.02410.0954-0.18640.24840.0286-0.097-00.262-0.00090.18870.5995-0.20090.8832-175.1818-53.927830.8709
280.0287-0.02730.01230.0163-0.03290.0524-0.15790.36650.0356-0.9594-0.08320.25490.263-0.22250-0.3821-0.0262-0.0450.4207-0.16230.375-144.3324-79.8819-7.5189
290.101-0.0103-0.01110.064-0.06660.12940.0815-0.19280.34050.1221-0.03030.0468-0.2677-0.016402.675-0.0301-0.04432.2002-0.17682.2977-65.133120.1773122.5208
300.61930.0478-0.35660.7148-0.23920.89390.0531-0.53620.10130.43880.12990.0093-0.32580.445901.6693-0.433-0.1331.5556-0.28860.5073-29.4519129.9226172.8961
310.40480.05890.06020.3469-0.06250.23280.260.55270.0128-0.75450.1187-0.13330.33590.258103.859-0.00850.17084.1526-0.11294.0259-116.669771.564152.5797
320.022-0.0031-0.00240.013-0.02570.06620.05050.0197-0.1579-0.03190.01250.08790.0862-0.008501.6893-0.684-0.00941.346-0.04610.8219-60.8578114.1315112.6478
330.1731-0.0162-0.03790.1650.04630.1148-0.02510.27570.4033-0.24530.00450.4423-0.287-0.458302.2413-0.08380.13522.59190.05832.2588-30.1343190.800991.2606
340.0105-0.0026-0.00360.0111-0.00340.0091-0.00630.02180.00110.0028-0.04420.01910.0172-0.017901.41110.0329-0.09571.241-0.19871.2357-110.3059123.9796135.5501
350.15570.04370.00110.21850.02980.1827-0.10330.194-0.0684-0.1699-0.1359-0.01580.052-0.025103.25340.04420.21963.2023-0.19223.3616-121.3668151.9799118.0966
360.2725-0.1044-0.01310.18610.03010.2042-0.0080.0063-0.0546-0.11180.0440.1901-0.0558-0.0536-02.91660.60870.43173.060.00453.106-129.0378169.7888146.4391
370.0910.0086-0.06930.0830.02990.1875-0.12710.1538-0.0792-0.0091-0.11330.40290.1796-0.2395-01.9553-0.02260.38552.73260.09742.1074-113.428127.7878179.2565
380.1165-0.01520.04980.13360.05890.05950.14110.20820.1358-0.26370.0157-0.0485-0.11960.002702.62090.07380.36662.32770.05512.201611.191227.253389.0105
390.0296-0.0310.01510.0176-0.00070.02270.0459-0.1131-0.12970.1607-0.01540.07970.1031-0.1825-01.01280.26950.22881.21920.14691.2224-119.871417.7406-1.5121
400.04860.03220.02150.0207-0.00970.04460.0137-0.09980.01260.05510.00910.1256-0.0426-0.0164-01.5195-0.2501-0.13851.2723-0.17971.2764-72.7467113.616289.4624
410.02-0.0034-0.00380.0112-0.00140.014-0.00790.0434-0.1559-0.0331-0.02430.04830.1808-0.1221-00.96540.07070.01271.23020.06271.2237-124.949611.2564-25.4226
420.02880.00340.00910.0268-0.0020.02070.0191-0.15290.03270.09520.08930.0717-0.005-0.055701.8401-0.13090.07911.7691-0.24111.5876-82.178490.943996.2175
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1((chain AA and (resid 2:8 or resid 27:557)) or (chain AD and resid 1:205) or (chain AP and resid 1:82) or (chain AT and resid 2:86) or (chain AL and resid 22:123) or (chain ZQ and (resid 299 or resid 315 or resid 362 or resid 417 or resid 509 o1
2X-RAY DIFFRACTION2((chain AA and (resid 9:26 or resid 558:562 or resid 564:566 or resid 914:925 or resid 1391:1396)) or (chain AL and resid 1:21) or (chain ZQ and (resid 21 or resid 560)))
3X-RAY DIFFRACTION3(chain AE and resid 9:73)
4X-RAY DIFFRACTION4(chain AE and resid 74:149)
5X-RAY DIFFRACTION5(chain AB and (resid 8:107 or resid 147:225))
6X-RAY DIFFRACTION6(chain AB and resid 108:146)
7X-RAY DIFFRACTION7((chain AA and (resid 926:986 or resid 1048:1390)) or (chain AC and resid 1:206) or (chain AG and resid 1:151) or (chain AI and resid 3:129) or (chain AJ and resid 5:102) or (chain AM and resid 1:114) or (chain AN and resid 1:100) or (chain A7
8X-RAY DIFFRACTION8(chain AA and resid 987:1047)
9X-RAY DIFFRACTION9((chain AA and (resid 563 or resid 567:913 or resid 1496:1530)) or (chain AF and resid 1:100) or (chain AH and resid 1:129) or (chain AK and resid 12:128) or (chain AO and resid 1:88) or (chain AR and resid 19:73) or (chain AU and resid 3:53)9
10X-RAY DIFFRACTION10((chain AA and resid 1397:1495) or (chain ZQ and (resid 1417 or resid 8201)))
11X-RAY DIFFRACTION11((chain CA and (resid 2:8 or resid 27:557)) or (chain CD and resid 1:205) or (chain CP and resid 1:82) or (chain CT and resid 2:86) or (chain CL and resid 22:123) or (chain ZU and (resid 299 or resid 315 or resid 362 or resid 417 or resid 509 o11
12X-RAY DIFFRACTION12((chain CA and (resid 9:26 or resid 558:562 or resid 564:566 or resid 914:925 or resid 1391:1396)) or (chain CL and resid 1:21) or (chain ZU and (resid 21 or resid 560)))
13X-RAY DIFFRACTION13(chain CE and resid 9:73)
14X-RAY DIFFRACTION14(chain CE and resid 74:149)
15X-RAY DIFFRACTION15(chain CB and (resid 8:107 or resid 147:225))
16X-RAY DIFFRACTION16(chain CB and resid 108:146)
17X-RAY DIFFRACTION17((chain CA and (resid 926:986 or resid 1048:1390)) or (chain CC and resid 1:206) or (chain CG and resid 1:151) or (chain CI and resid 3:129) or (chain CJ and resid 5:102) or (chain CM and resid 1:114) or (chain CN and resid 1:100) or (chain C17
18X-RAY DIFFRACTION18(chain CA and resid 987:1047)
19X-RAY DIFFRACTION19((chain CA and (resid 563 or resid 567:913 or resid 1496:1530)) or (chain CF and resid 1:100) or (chain CH and resid 1:129) or (chain CK and resid 12:128) or (chain CO and resid 1:88) or (chain CR and resid 19:73) or (chain CU and resid 3:53)19
20X-RAY DIFFRACTION20((chain CA and resid 1397:1495) or (chain ZU and (resid 1417 or resid 8201)))
21X-RAY DIFFRACTION21((chain BA and (resid 1:876 or resid 902:1038 or resid 1116:1905 or resid 1930:2092 or resid 2198:2296 or resid 2322:2903)) or (chain BC and resid 1:271) or (chain BS and resid 1:110) or (chain BT and resid 1:93) or (chain BU and resid 1:102) o21
22X-RAY DIFFRACTION22((chain BA and resid 1039:1115) or (chain BI and resid 1:141))
23X-RAY DIFFRACTION23(chain BA and resid 1906:1929)
24X-RAY DIFFRACTION24(chain BA and resid 2093:2197)
25X-RAY DIFFRACTION25(chain BA and resid 877:901)
26X-RAY DIFFRACTION26((chain BA and resid 2297:2321) or (chain BF and resid 1:177) or (chain ZR and resid 2304))
27X-RAY DIFFRACTION27((chain BB and (resid 2:69 or resid 108:119)) or (chain BO and resid 2:117))
28X-RAY DIFFRACTION28((chain BB and resid 70:107) or (chain BV and resid 1:94) or (chain ZR and resid 6095:6100))
29X-RAY DIFFRACTION29(chain BH and resid 49:149)
30X-RAY DIFFRACTION30((chain DA and (resid 1:876 or resid 902:1038 or resid 1116:1905 or resid 1930:2092 or resid 2198:2296 or resid 2322:2903)) or (chain DC and resid 1:271) or (chain DS and resid 1:110) or (chain DT and resid 1:93) or (chain DU and resid 1:102) o30
31X-RAY DIFFRACTION31((chain DA and resid 1039:1115) or (chain DI and resid 1:141))
32X-RAY DIFFRACTION32(chain DA and resid 1906:1929)
33X-RAY DIFFRACTION33(chain DA and resid 2093:2197)
34X-RAY DIFFRACTION34(chain DA and resid 877:901)
35X-RAY DIFFRACTION35((chain DA and resid 2297:2321) or (chain DF and resid 1:178) or (chain ZT and resid 2304))
36X-RAY DIFFRACTION36((chain DB and (resid 2:69 or resid 108:119)) or (chain DO and resid 2:117))
37X-RAY DIFFRACTION37((chain DB and resid 70:107) or (chain DV and resid 1:94) or (chain ZT and resid 6095:6100))
38X-RAY DIFFRACTION38(chain DH and resid 49:149)
39X-RAY DIFFRACTION39(chain I or chain J)
40X-RAY DIFFRACTION40(chain K or chain L)
41X-RAY DIFFRACTION41(chain M)
42X-RAY DIFFRACTION42(chain N)

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