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Open data
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Basic information
Entry | Database: PDB / ID: 4s2s | ||||||
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Title | Crystal Structure of Fab fragment of monoclonal antibody RoAb13 | ||||||
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![]() | IMMUNE SYSTEM / Immunoglobulin | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Chain, B. / Arnold, J. / Akthar, S. / Noursadeghi, M. / Lapp, T. / Ji, C. / Naider, D. / Zhang, Y. / Govada, L. / Saridakis, E. / Chayen, N.E. | ||||||
![]() | ![]() Title: A Linear Epitope in the N-Terminal Domain of CCR5 and Its Interaction with Antibody. Authors: Chain, B. / Arnold, J. / Akthar, S. / Brandt, M. / Davis, D. / Noursadeghi, M. / Lapp, T. / Ji, C. / Sankuratri, S. / Zhang, Y. / Govada, L. / Saridakis, E. / Chayen, N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 197 KB | Display | ![]() |
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PDB format | ![]() | 156.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.6 KB | Display | ![]() |
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Full document | ![]() | 468.4 KB | Display | |
Data in XML | ![]() | 41.9 KB | Display | |
Data in CIF | ![]() | 62.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1hilS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 24063.961 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Antibody | Mass: 24169.846 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.39 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 20% PEG 2000 monomethyl ether, 0.01M nickel chloride, 0.1M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 944+ / Detector: CCD / Date: May 15, 2012 |
Radiation | Monochromator: Cu / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.07→15.45 Å / Num. all: 58588 / Num. obs: 55876 / % possible obs: 95.4 % / Observed criterion σ(I): -3.7 |
Reflection shell | Resolution: 2.07→2.21 Å / % possible all: 89.12 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1HIL Resolution: 2.1→15.402 Å / SU ML: 0.29 / σ(F): 0 / Phase error: 24.66 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→15.402 Å
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Refine LS restraints |
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LS refinement shell |
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