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Yorodumi- PDB-4qsz: Crystal structure of mouse JMJd7 fused with maltose-binding protein -
+Open data
-Basic information
Entry | Database: PDB / ID: 4qsz | ||||||
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Title | Crystal structure of mouse JMJd7 fused with maltose-binding protein | ||||||
Components | Maltose-binding periplasmic protein, JmjC domain-containing protein 7 chimera | ||||||
Keywords | TRANSCRIPTION / Demethylase | ||||||
Function / homology | Function and homology information peptidyl-lysine (3S)-dioxygenase / peptidyl-lysine 3-dioxygenase activity / Protein hydroxylation / 2-oxoglutarate-dependent dioxygenase activity / Hydrolases; Acting on peptide bonds (peptidases) / detection of maltose stimulus / maltose binding / maltose transport complex / maltose transport / maltodextrin transmembrane transport ...peptidyl-lysine (3S)-dioxygenase / peptidyl-lysine 3-dioxygenase activity / Protein hydroxylation / 2-oxoglutarate-dependent dioxygenase activity / Hydrolases; Acting on peptide bonds (peptidases) / detection of maltose stimulus / maltose binding / maltose transport complex / maltose transport / maltodextrin transmembrane transport / carbohydrate transmembrane transporter activity / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / carbohydrate transport / aminopeptidase activity / methylated histone binding / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / monooxygenase activity / outer membrane-bounded periplasmic space / endopeptidase activity / periplasmic space / DNA damage response / proteolysis / membrane / metal ion binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli K-12 (bacteria) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.86 Å | ||||||
Authors | Liu, H. / Wang, C. / Zhang, G.Y. | ||||||
Citation | Journal: To be Published Title: Crystal structure of mouse JMJd7 fused with maltose-binding protein Authors: Liu, H. / Zhang, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4qsz.cif.gz | 269.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4qsz.ent.gz | 222.4 KB | Display | PDB format |
PDBx/mmJSON format | 4qsz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qs/4qsz ftp://data.pdbj.org/pub/pdb/validation_reports/qs/4qsz | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 76557.555 Da / Num. of mol.: 2 / Fragment: SEE REMARK 999 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria), (gene. exp.) Mus musculus (house mouse) Gene: malE, b4034, JW3994, Jmjd7 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AEX9, UniProt: P0C872 #2: Sugar | ChemComp-GLC / #3: Chemical | Sequence details | PROTEIN IS A CHIMERA COMPRISING RESIDUES 27-387 OF MBP (UNP P0AEX9) AND RESIDUES 2-312 OF JMJD7 ...PROTEIN IS A CHIMERA COMPRISING | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.59 % |
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Crystal grow | Temperature: 277 K / Method: evaporation / pH: 8 Details: Hampton Research PEG/ION HT (89): 20 mM sodium citrate, 80 mM Bis-Tris propane, 16% PEG3350, pH 8.0, EVAPORATION, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 4.2.2 / Wavelength: 1 Å |
Detector | Type: NOIR-1 / Detector: CCD / Date: Mar 28, 2014 |
Radiation | Monochromator: Rosenbaum-Rock Si(111) sagitally focused / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.59→100 Å / Num. all: 46852 / Num. obs: 44978 / % possible obs: 96 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 |
Reflection shell | Resolution: 2.59→2.68 Å / % possible all: 91 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.86→62.153 Å / SU ML: 0.39 / σ(F): 1.04 / Phase error: 26 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.86→62.153 Å
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Refine LS restraints |
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LS refinement shell |
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