+Open data
-Basic information
Entry | Database: PDB / ID: 4oyc | ||||||
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Title | Crystal structure of the PrgK periplasmic domain 2 | ||||||
Components | Lipoprotein PrgK | ||||||
Keywords | PROTEIN TRANSPORT / T3SS / macromolecular assembly / inner-membrane | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Salmonella typhimurium (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||
Authors | Bergeron, J.R.C. / Strynadka, N.C.J. | ||||||
Citation | Journal: Structure / Year: 2015 Title: The modular structure of the inner-membrane ring component PrgK facilitates assembly of the type III secretion system basal body. Authors: Julien R C Bergeron / Liam J Worrall / Soumya De / Nikolaos G Sgourakis / Adrienne H Cheung / Emilie Lameignere / Mark Okon / Gregory A Wasney / David Baker / Lawrence P McIntosh / Natalie C J Strynadka / Abstract: The type III secretion system (T3SS) is a large macromolecular assembly found at the surface of many pathogenic Gram-negative bacteria. Its role is to inject toxic "effector" proteins into the cells ...The type III secretion system (T3SS) is a large macromolecular assembly found at the surface of many pathogenic Gram-negative bacteria. Its role is to inject toxic "effector" proteins into the cells of infected organisms. The molecular details of the assembly of this large, multimembrane-spanning complex remain poorly understood. Here, we report structural, biochemical, and functional analyses of PrgK, an inner-membrane component of the prototypical Salmonella typhimurium T3SS. We have obtained the atomic structures of the two ring building globular domains and show that the C-terminal transmembrane helix is not essential for assembly and secretion. We also demonstrate that structural rearrangement of the two PrgK globular domains, driven by an interconnecting linker region, may promote oligomerization into ring structures. Finally, we used electron microscopy-guided symmetry modeling to propose a structural model for the intimately associated PrgH-PrgK ring interaction within the assembled basal body. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4oyc.cif.gz | 47.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4oyc.ent.gz | 32.8 KB | Display | PDB format |
PDBx/mmJSON format | 4oyc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oy/4oyc ftp://data.pdbj.org/pub/pdb/validation_reports/oy/4oyc | HTTPS FTP |
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-Related structure data
Related structure data | 2mkyC 3j6dC 4w4mC 1yj7S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | Oligomeric state is 24-mer as determined by Electron Microscopy |
-Components
#1: Protein | Mass: 12007.386 Da / Num. of mol.: 2 / Fragment: 96-200 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella typhimurium (bacteria) / Strain: LT2 / SGSC1412 / ATCC 700720 / Gene: prgK, STM2871 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P41786 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.67 Å3/Da / Density % sol: 26.4 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 100 mM sodium acetate pH 5.5, 20 % PEG 6000, 50 mM NaCl, 50 mM MgCl2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: CLSI / Beamline: 08B1-1 / Wavelength: 0.98 Å |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: Jun 22, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→36.09 Å / Num. obs: 4992 / % possible obs: 99.9 % / Redundancy: 3.5 % / Biso Wilson estimate: 54.04 Å2 / Net I/σ(I): 4.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1YJ7 Resolution: 2.6→36.09 Å / Cor.coef. Fo:Fc: 0.8703 / Cor.coef. Fo:Fc free: 0.8491 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.341
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Displacement parameters | Biso mean: 48.68 Å2
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Refine analyze | Luzzati coordinate error obs: 0.381 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→36.09 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.91 Å / Total num. of bins used: 5
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