PDB-4nwn: Computationally Designed Two-Component Self-Assembling Tetrahedra...

ID or keywords:

Entry

Database: PDB / ID: 4nwn

Title

Computationally Designed Two-Component Self-Assembling Tetrahedral Cage T32-28

Components

Propanediol utilization: polyhedral bodies pduT
Uncharacterized protein

Keywords

PROTEIN BINDING / two-component / self-assembling / tetrahedron / designed protein cage / computational design / protein engineering / multimerization / nanomaterial / nanostructure

Function / homology

Periplasmic metal-binding protein Tp34-type / Periplasmic metal-binding protein Tp34-type superfamily / Fe2+ transport protein / metal ion binding / Iron transporter / Uncharacterized protein / :

Function and homology information

Biological species

Campylobacter jejuni (Campylobacter)

Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT /

molecular replacement / Resolution: 4.5 Å

Authors

McNamara, D.E. / King, N.P. / Bale, J.B. / Sheffler, W. / Baker, D. / Yeates, T.O.

Citation

Journal: Nature / Year: 2014
Title: Accurate design of co-assembling multi-component protein nanomaterials.
Authors: King, N.P. / Bale, J.B. / Sheffler, W. / McNamara, D.E. / Gonen, S. / Gonen, T. / Yeates, T.O. / Baker, D.

History

Deposition	Dec 6, 2013	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	May 28, 2014	Provider: repository / Type: Initial release
Revision 1.1	Jun 11, 2014	Group: Database references
Revision 1.2	Sep 20, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ref_seq_dif Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	4nwn.cif.gz	1.1 MB	Display	PDBx/mmCIF format
PDB format	pdb4nwn.ent.gz	909.3 KB	Display	PDB format
PDBx/mmJSON format	4nwn.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	4nwn_validation.pdf.gz	570 KB	Display	wwPDB validaton report
Full document	4nwn_full_validation.pdf.gz	572.3 KB	Display
Data in XML	4nwn_validation.xml.gz	112.5 KB	Display
Data in CIF	4nwn_validation.cif.gz	157.5 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/nw/4nwn ftp://data.pdbj.org/pub/pdb/validation_reports/nw/4nwn	HTTPS FTP

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Related structure data

Related structure data	4nwoC 4nwpC 4nwqC 4nwrC 3lzlS 3n79S C: citing same article (ref.) S: Starting model for refinement
Similar structure data	23266 5jcg-1 Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#165 - Sep 2013 Designed Protein Cages similarity (1)

Deposited unit

A: Uncharacterized protein

B: Propanediol utilization: polyhedral bodies pduT

C: Uncharacterized protein

D: Propanediol utilization: polyhedral bodies pduT

E: Uncharacterized protein

F: Propanediol utilization: polyhedral bodies pduT

G: Uncharacterized protein

H: Propanediol utilization: polyhedral bodies pduT

I: Uncharacterized protein

J: Propanediol utilization: polyhedral bodies pduT

K: Uncharacterized protein

L: Propanediol utilization: polyhedral bodies pduT

M: Uncharacterized protein

N: Propanediol utilization: polyhedral bodies pduT

O: Uncharacterized protein

P: Propanediol utilization: polyhedral bodies pduT

Q: Uncharacterized protein

R: Propanediol utilization: polyhedral bodies pduT

S: Uncharacterized protein

T: Propanediol utilization: polyhedral bodies pduT

U: Uncharacterized protein

V: Propanediol utilization: polyhedral bodies pduT

W: Uncharacterized protein

X: Propanediol utilization: polyhedral bodies pduT

447 kDa, 24 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author

Unit cell

Length a, b, c (Å)	246.010, 246.010, 290.940
Angle α, β, γ (deg.)	90.000, 90.000, 120.000
Int Tables number	152
Space group name H-M	P3₁21

Details

The biological assembly is a protein cage with tetrahedral point group symmetry that comprises the contents of one asymmetric units.

#1: Protein	Uncharacterized protein Mass: 19928.221 Da / Num. of mol.: 12 Mutation: R114L, S145L, N148A, N149A, T152A, V153T, E156L, S157A, E160A, R167A, V169I Source method: isolated from a genetically manipulated source Source: (gene. exp.) Campylobacter jejuni (Campylobacter) / Strain: 81-176 / Gene: CJE1831, CJJ81176_1650, PduT, stm2054 / Plasmid: pET29b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: K8VQB8
#2: Protein	Propanediol utilization: polyhedral bodies pduT Mass: 17315.658 Da / Num. of mol.: 12 Mutation: E71A, K73A, T75A, R82T, N114T, E116A, Y120L, K147V, D149T, K151F Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) Strain: STm5 / Gene: B581_12628, cjj81176_1650 / Plasmid: pET29b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A1W1R1, UniProt: A0A0H3PA01*PLUS

#1: Protein

Uncharacterized protein

Mass: 19928.221 Da / Num. of mol.: 12
Mutation: R114L, S145L, N148A, N149A, T152A, V153T, E156L, S157A, E160A, R167A, V169I
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Campylobacter jejuni (Campylobacter) / Strain: 81-176 / Gene: CJE1831, CJJ81176_1650, PduT, stm2054 / Plasmid: pET29b / Production host:

Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: K8VQB8

#2: Protein

Propanediol utilization: polyhedral bodies pduT

Mass: 17315.658 Da / Num. of mol.: 12
Mutation: E71A, K73A, T75A, R82T, N114T, E116A, Y120L, K147V, D149T, K151F
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Strain: STm5 / Gene: B581_12628, cjj81176_1650 / Plasmid: pET29b / Production host:

Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A1W1R1, UniProt: A0A0H3PA01*PLUS

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 5.69 Å³/Da / Density % sol: 78.37 %
Crystal grow	Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 100 mM sodium citrate tribasic dihydrate pH 5.6, 200 mM ammonium acetate, 24% (v/v) (+/-)-2-methyl-2,4-pentanediol, vapor diffusion, hanging drop, temperature 298K

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

SYNCHROTRON / Site:

APS

/ Beamline: 24-ID-C / Wavelength: 0.9793 Å

Detector

Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 11, 2013

Radiation

Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.9793 Å / Relative weight: 1

Reflection

Resolution: 4.5→93.927 Å / Num. all: 60851 / Num. obs: 59814 / % possible obs: 98.3 % / Observed criterion σ(I): -3 / Redundancy: 7.3 % / B_iso Wilson estimate: 183.99 Å² / R_merge(I) obs: 0.136 / Net I/σ(I): 13.08

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Mean I/σ(I) obs	Num. measured obs	Num. unique all	Num. unique obs	% possible all
4.5-4.62	7.45	0.974	2.11	32171	32171	4321	97.3
4.62-4.74	7.51	0.82	2.51	32439	32439	4318	99.4
4.74-4.88	7.51	0.701	2.97	31465	31465	4189	99.1
4.88-5.03	7.5	0.695	3.04	30410	30410	4056	99
5.03-5.2	7.39	0.546	3.75	29129	29189	3942	99
5.2-5.38	7.32	0.465	4.36	27849	27849	3803	98.9
5.38-5.58	7.18	0.447	4.48	26476	26476	3687	98.7
5.58-5.81	6.8	0.404	4.81	23763	23763	3495	97.9
5.81-6.07	6.98	0.357	5.47	23663	23663	3390	97.9
6.07-6.36	7.64	0.319	6.39	24767	24767	3242	98.8
6.36-6.71	7.54	0.238	8.43	23390	23390	3104	98.7
6.71-7.11	7.48	0.173	11.23	21769	21769	2912	98.4
7.11-7.6	7.35	0.112	16.6	20448	20448	2783	98.6
7.6-8.21	7.12	0.072	24.36	18243	18243	2561	97.8
8.21-9	6.49	0.049	32.8	15114	15114	2329	96.5
9-10.06	7.56	0.041	41.38	16272	16272	2152	97.9
10.06-11.62	7.4	0.034	48.52	14165	14165	1913	97.9
11.62-14.23	7.18	0.029	54.68	11711	11711	1631	97.8
14.23-20.12	6.45	0.027	54.98	8079	8079	1252	95.1
20.12-93.927	7.07	0.023	68.27	5187	5187	734	95.1

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Phasing

Phasing	Method: molecular replacement
Phasing MR	Model details: Phaser MODE: MR_AUTO

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Processing

Software

Name	Version	Classification
XSCALE		data scaling
PHASER	2.5.5	phasing
PHENIX	1.8.4_1496	refinement
PDB_EXTRACT	3.11	data extraction
XDS		data reduction

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: Model of 24-mer based on 3LZL and 3N79

3lzl

3n79

Resolution: 4.5→93.927 Å / Occupancy max: 1 / Occupancy min: 1 / FOM work R set: 0.6573 / SU ML: 0.95 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 39.47 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.3429	5981	10 %	RANDOM
R_work	0.2971	-	-	-
obs	0.3017	59810	98.31 %	-
all	-	60851	-	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso max: 577.77 Å² / B_iso mean: 216.1558 Å² / B_iso min: 52.54 Å²

Refinement step

Cycle: LAST / Resolution: 4.5→93.927 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	20307	0	0	0	20307

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.003	20475
X-RAY DIFFRACTION	f_angle_d	0.546	28509
X-RAY DIFFRACTION	f_chiral_restr	0.016	3623
X-RAY DIFFRACTION	f_plane_restr	0.004	4243
X-RAY DIFFRACTION	f_dihedral_angle_d	7.605	4627

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all	Num. reflection obs	% reflection obs (%)
4.4992-4.5503	0.3785	192	0.3758	1725	1917	1725	95
4.5503-4.6038	0.4184	198	0.3465	1778	1976	1778	100
4.6038-4.66	0.3802	201	0.3512	1809	2010	1809	99
4.66-4.719	0.4043	198	0.3467	1782	1980	1782	99
4.719-4.7811	0.3793	198	0.3441	1790	1988	1790	99
4.7811-4.8465	0.3852	199	0.3316	1783	1982	1783	99
4.8465-4.9158	0.3849	198	0.3409	1789	1987	1789	99
4.9158-4.9892	0.4051	196	0.3514	1757	1953	1757	99
4.9892-5.0671	0.4165	199	0.3614	1799	1998	1799	99
5.0671-5.1502	0.4148	205	0.3496	1841	2046	1841	99
5.1502-5.239	0.4124	196	0.3476	1760	1956	1760	99
5.239-5.3342	0.3701	201	0.3627	1810	2011	1810	99
5.3342-5.4368	0.406	196	0.3508	1767	1963	1767	99
5.4368-5.5478	0.4232	199	0.3593	1792	1991	1792	99
5.5478-5.6684	0.4085	199	0.3494	1789	1988	1789	99
5.6684-5.8002	0.4249	196	0.3732	1763	1959	1763	97
5.8002-5.9453	0.4236	195	0.3736	1762	1957	1762	97
5.9453-6.106	0.4254	200	0.3545	1797	1997	1797	99
6.106-6.2856	0.4167	200	0.3627	1797	1997	1797	99
6.2856-6.4885	0.4181	199	0.3318	1789	1988	1789	98
6.4885-6.7203	0.4082	201	0.3377	1809	2010	1809	99
6.7203-6.9893	0.3266	201	0.3163	1810	2011	1810	99
6.9893-7.3073	0.3193	198	0.2721	1782	1980	1782	98
7.3073-7.6924	0.2858	202	0.2578	1816	2018	1816	98
7.6924-8.1741	0.2898	200	0.2274	1805	2005	1805	98
8.1741-8.8047	0.2467	197	0.2028	1773	1970	1773	96
8.8047-9.69	0.2378	200	0.2028	1802	2002	1802	98
9.69-11.0902	0.2485	203	0.214	1825	2028	1825	98
11.0902-13.9652	0.304	205	0.2372	1840	2045	1840	98
13.9652-93.9478	0.3909	209	0.3719	1888	2097	1888	96

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	6.3415	0.1569	-1.2096	3.6634	-0.8514	2.8809	-0.0646	0.426	-0.9967	0.3144	0.0469	1.8851	-0.4704	-0.2688	0.0198	1.1567	0.2012	0.2024	0.3181	-0.6084	0.5144	139.2699	-34.0755	67.9931
2	5.5887	2.2454	1.5011	4.2649	1.134	2.2968	-0.2413	2.1017	0.5608	0.5278	0.6584	0.4757	-0.0508	0.4494	-0.0553	0.863	-0.0687	0.2056	1.3977	0.2792	1.353	157.0791	-39.7787	44.558
3	3.9598	0.6106	1.1804	4.031	1.3676	0.7284	-0.6126	0.8859	0.1911	0.2259	1.0314	-0.4671	-1.6145	0.0498	-0.0226	3.0658	0.3566	-0.0856	0.8682	0.427	0.5048	138.8186	-0.7198	72.2998
4	2.4684	-1.0181	-1.2397	4.3282	-0.8045	5.7631	-0.8234	-0.2514	-0.6105	0.7803	0.1199	0.7651	-1.0288	0.0874	1.5059	2.3114	-0.2453	-0.3545	0.7456	0.2501	2.437	128.9439	27.1131	70.5792
5	1.7358	2.7337	-0.7352	8.9482	0.6996	1.1556	-0.1951	0.3425	0.1951	-2.0553	-0.4092	-0.7709	0.1109	-0.3939	0.1992	1.8026	0.1317	-0.1362	0.7532	0.1746	0.8162	114.5921	-20.0032	85.6558
6	0.9881	0.4631	-0.1118	2.8338	0.3582	2.7197	-0.1224	0.0767	-0.6887	-0.3107	0.745	-0.5255	1.4544	-0.0279	0.9565	2.4764	-0.3842	1.4473	0.408	0.7254	0.3717	91.479	-39.3342	85.7493
7	3.3935	-2.2173	-0.011	7.4296	-4.8939	4.2236	-0.3794	0.6233	0.6207	-0.3013	0.0859	-0.8178	1.4489	-0.43	0.1569	1.6191	0.0399	-0.0039	2.4056	-0.6411	1.2878	139.5506	-46.7336	-1.1239
8	1.4118	-1.2353	-0.0489	3.8154	0.9055	4.8014	-0.4781	0.6053	-0.2991	0.727	0.534	0.7113	0.6601	0.0741	0.5644	0.7945	0.1206	-0.1174	2.0324	-0.774	1.521	146.4331	-58.1399	26.349
9	1.6888	0.8946	0.5409	0.4684	0.5811	0.9031	-1.0645	0.4858	-0.4675	-0.4373	-0.5355	1.1275	-0.2904	-1.3182	-2.4333	1.0436	-0.6575	-0.6673	1.691	-2.6794	2.0562	121.1924	-31.5467	-24.8015
10	1.42	0.5346	0.2178	3.5791	0.3195	1.0766	0.8659	-0.4271	-0.036	-1.6574	-0.5305	-1.0499	-0.9952	1.1732	0.6384	2.0034	-0.3405	1.0524	2.7701	-0.1011	2.1515	133.4044	-3.6882	-27.92
11	3.7545	-0.4676	2.309	2.3387	0.3659	2.9301	-0.2042	-0.8253	0.4848	1.0114	0.7528	-1.513	0.1675	0.9826	-0.0075	0.6599	0.6783	-0.2881	2.1104	-1.5547	0.9173	108.9303	-58.2355	-8.4963
12	3.578	1.2501	-2.4527	2.1144	0.5798	2.9392	0.0006	1.0472	0.9003	-1.2771	0.7171	0.6006	-0.4476	-0.8191	0.7244	1.914	0.0599	-1.3054	1.6292	-1.2922	3.1335	78.9894	-57.7238	-12.3681
13	4.1399	-1.029	1.1534	3.6013	-0.6294	1.4246	-0.4265	0.2904	0.7394	0.1919	0.4862	0.9549	-0.4193	-0.5065	0.4623	1.3794	0.7754	0.3038	0.8713	1.2238	2.5571	111.4196	43.959	29.3521
14	3.9569	0.4397	0.6565	2.7196	-1.1841	2.6015	0.0931	0.7341	0.0163	1.3527	-0.6931	-1.8198	0.017	0.8279	0.8778	1.6177	-0.1997	0.4938	0.5795	0.6772	2.1205	135.4081	38.826	45.7915
15	1.6285	0.8096	0.7337	1.9016	-0.2554	3.7677	0.3627	-0.1112	0.0372	-1.0811	-1.0339	-0.0009	0.3371	-0.4178	-0.2968	2.8941	0.935	0.0884	2.2584	0.061	0.2915	117.3111	33.9699	-2.0889
16	1.3432	0.0964	0.8677	2.7076	1.99	1.9873	0.6343	0.3822	1.862	-2.7747	0.3256	1.2486	0.1423	-1.0767	-0.2351	2.7236	0.5713	0.9518	3.1596	1.3984	3.0163	115.1215	17.5292	-27.2079
17	2.3409	-0.4811	0.7374	1.4735	0.6211	2.9275	0.3919	1.6762	-0.8792	-0.8472	-0.6022	-0.545	-0.6107	1.4143	-0.5107	1.8596	-0.1175	-0.137	3.0335	0.0445	0.35	85.3496	38.8819	8.4549
18	0.8144	1.4035	-0.4755	3.7137	-1.305	0.4543	-0.637	0.9204	-0.9333	-0.387	0.457	0.8955	0.167	1.8654	0.4578	1.6957	0.3844	-0.1841	2.9709	-0.7061	1.549	60.3906	35.5451	24.9979
19	1.1698	-0.6655	0.0331	1.9383	1.2532	5.269	-0.3814	0.1549	-0.4961	-0.02	1.5502	0.8003	0.3506	-1.2723	1.1881	2.4225	-0.1695	1.5563	1.1794	0.1067	2.7904	54.495	-35.7683	54.0393
20	2.8842	-1.4664	0.4065	2.3097	1.255	3.964	0.7354	-1.0074	-0.0803	0.8084	-0.0644	1.3026	0.0818	0.178	-0.1055	1.5834	-0.3654	1.227	1.7284	-0.3869	1.7259	66.6108	-27.3816	80.1575
21	0.1209	-0.0909	-0.0519	0.9119	0.8299	0.754	-0.436	0.2467	-0.1381	-0.0278	0.1425	0.4333	0.9085	-0.5692	0.0134	3.5694	0.4448	-1.201	1.738	-2.0121	3.5091	48.8854	-36.915	21.0089
22	3.3886	-0.3927	-0.1946	3.3954	-1.1692	8.2351	-0.9681	0.7176	-2.2841	0.1101	-0.3268	0.5863	-0.6867	0.1498	0.2998	1.2773	-0.2343	0.2011	1.7766	-0.7665	3.0052	63.922	-61.7068	11.0418
23	0.6916	-0.7853	0.6722	3.7961	-0.9954	5.4743	-0.6075	-3.0967	1.7211	0.5072	1.2541	-0.3379	0.6457	1.59	0.1631	1.5481	-0.2196	0.1214	3.1684	-1.087	2.3119	43.9618	-8.4557	37.7709
24	4.4139	0.4368	-2.8331	4.1118	-2.8208	3.9153	-0.0009	1.7139	0.4916	-0.7196	1.5603	0.797	0.6832	0.5326	-1.2874	1.7137	0.5286	-0.6769	2.2453	-0.814	2.608	44.5016	13.7351	16.9009

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 2 through 183 )	A
2	X-RAY DIFFRACTION	2	chain 'B' and (resid 2 through 158 )	B
3	X-RAY DIFFRACTION	3	chain 'C' and (resid 2 through 184 )	C
4	X-RAY DIFFRACTION	4	chain 'D' and (resid 2 through 158 )	D
5	X-RAY DIFFRACTION	5	chain 'E' and (resid 2 through 184 )	E
6	X-RAY DIFFRACTION	6	chain 'F' and (resid 2 through 158 )	F
7	X-RAY DIFFRACTION	7	chain 'G' and (resid 2 through 184 )	G
8	X-RAY DIFFRACTION	8	chain 'H' and (resid 2 through 158 )	H
9	X-RAY DIFFRACTION	9	chain 'I' and (resid 2 through 183 )	I
10	X-RAY DIFFRACTION	10	chain 'J' and (resid 2 through 158 )	J
11	X-RAY DIFFRACTION	11	chain 'K' and (resid 2 through 184 )	K
12	X-RAY DIFFRACTION	12	chain 'L' and (resid 2 through 158 )	L
13	X-RAY DIFFRACTION	13	chain 'M' and (resid 2 through 182 )	M
14	X-RAY DIFFRACTION	14	chain 'N' and (resid 2 through 158 )	N
15	X-RAY DIFFRACTION	15	chain 'O' and (resid 2 through 183 )	O
16	X-RAY DIFFRACTION	16	chain 'P' and (resid 2 through 158 )	P
17	X-RAY DIFFRACTION	17	chain 'Q' and (resid 2 through 184 )	Q
18	X-RAY DIFFRACTION	18	chain 'R' and (resid 2 through 158 )	R
19	X-RAY DIFFRACTION	19	chain 'S' and (resid 2 through 184 )	S
20	X-RAY DIFFRACTION	20	chain 'T' and (resid 2 through 158 )	T
21	X-RAY DIFFRACTION	21	chain 'U' and (resid 2 through 184 )	U
22	X-RAY DIFFRACTION	22	chain 'V' and (resid 2 through 158 )	V
23	X-RAY DIFFRACTION	23	chain 'W' and (resid 2 through 184 )	W
24	X-RAY DIFFRACTION	24	chain 'X' and (resid 2 through 158 )	X

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